{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:39:52Z","timestamp":1791193192019,"version":"4.1.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2013,6,12]],"date-time":"2013-06-12T00:00:00Z","timestamp":1370995200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0066091","type":"journal-article","created":{"date-parts":[[2013,6,12]],"date-time":"2013-06-12T21:02:41Z","timestamp":1371070961000},"page":"e66091","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Slimmer or Fertile? Pharmacological Mechanisms Involved in Reduced Sperm Quality and Fertility in Rats Exposed to the Anorexigen Sibutramine"],"prefix":"10.1371","volume":"8","author":[{"given":"Cibele S.","family":"Borges","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Missassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enio S. A.","family":"Pacini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Ricardo A.","family":"Kiguti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marciana","family":"Sanabria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel F.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais P.","family":"Banzato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana E.","family":"Perobelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 S.","family":"Pupo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilma G.","family":"Kempinas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Robaire B HB, Orgebin-Crist MC (2006) The epididymis. In: Elsevier, editor. Knobil and Neill&apos;s Physiology of Reproduction: Neill JD. 1071\u20131148.","DOI":"10.1016\/B978-012515400-0\/50027-0"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1530\/jrf.0.0740497","article-title":"Enhancement of sperm transport through the rat epididymis after castration","volume":"74","author":"S Sujarit","year":"1985","journal-title":"J Reprod Fertil"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/j.1365-2605.2007.00788.x","article-title":"Effects of altered epididymal sperm transit time on sperm quality","volume":"31","author":"CD Fernandez","year":"2008","journal-title":"Int J Androl"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1016\/j.fertnstert.2012.03.025","article-title":"Morphometric-stereological and functional epididymal alterations and a decrease in fertility in rats treated with finasteride and after a 30-day post-treatment recovery period","volume":"97","author":"PV Garcia","year":"2012","journal-title":"Fertil Steril"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1566-0702(01)00292-2","article-title":"Identification of CNS neurons involved in the innervation of the epididymis: a viral transneuronal tracing study","volume":"92","author":"I Gerendai","year":"2001","journal-title":"Auton Neurosci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.1998.tb02463.x","article-title":"The autonomic innervation of the epididymis: its effects on epididymal function and fertility","volume":"19","author":"DD Ricker","year":"1998","journal-title":"J Androl"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1476-5381.1991.tb12207.x","article-title":"Sympathetic co-transmission to the cauda epididymis of the rat: characterization of postjunctional adrenoceptors and purinoceptors","volume":"102","author":"S Ventura","year":"1991","journal-title":"Br J Pharmacol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1046\/j.1365-2680.2000.00195.x","article-title":"The autonomic and sensory innervation of the smooth muscle of the prostate gland: a review of pharmacological and histological studies","volume":"20","author":"JN Pennefather","year":"2000","journal-title":"J Auton Pharmacol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1095\/biolreprod44.6.1171","article-title":"Requirement of innervation for maintenance of structural and functional integrity in the rat prostate","volume":"44","author":"JM Wang","year":"1991","journal-title":"Biol Reprod"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/j.1439-0272.1995.tb01081.x","article-title":"The hypophyseal-testicular axis and sex accessory glands following chemical sympathectomy with guanethidine of pre-pubertal to mature rats","volume":"27","author":"WG Kempinas","year":"1995","journal-title":"Andrologia"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1095\/biolreprod59.4.897","article-title":"Fertility of rat epididymal sperm after chemically and surgically induced sympathectomy","volume":"59","author":"WD Kempinas","year":"1998","journal-title":"Biol Reprod"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.taap.2009.05.021","article-title":"Multiple effects of sibutramine on ejaculation and on vas deferens and seminal vesicle contractility","volume":"239","author":"FD Nojimoto","year":"2009","journal-title":"Toxicol Appl Pharmacol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e39062","DOI":"10.1371\/journal.pone.0039062","article-title":"Effect of anti-obesity drug on cardiovascular risk factors: a systematic review and meta-analysis of randomized controlled trials","volume":"7","author":"YH Zhou","year":"2012","journal-title":"PLoS One"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1002\/pmic.201000664","article-title":"Proteome changes in rat plasma in response to sibutramine","volume":"11","author":"JW Choi","year":"2011","journal-title":"Proteomics"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1467-789x.2000.00020.x","article-title":"An assessment of the safety and efficacy of sibutramine, an anti-obesity drug with a novel mechanism of action","volume":"1","author":"E Nisoli","year":"2000","journal-title":"Obes Rev"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1038\/sj.bjp.0701311","article-title":"Investigation of the mechanisms underlying the hypophagic effects of the 5-HT and noradrenaline reuptake inhibitor, sibutramine, in the rat","volume":"121","author":"HC Jackson","year":"1997","journal-title":"Br J Pharmacol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1038\/oby.2011.51","article-title":"Inverse agonism at alpha2A adrenoceptors augments the hypophagic effect of sibutramine in rats","volume":"19","author":"SK Janhunen","year":"2011","journal-title":"Obesity (Silver Spring)"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.2174\/157015912799362788","article-title":"Sibutramine effects on central mechanisms regulating energy homeostasis","volume":"10","author":"JR Araujo","year":"2012","journal-title":"Curr Neuropharmacol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"S114","DOI":"10.2337\/dc11-s205","article-title":"Sibutramine on cardiovascular outcome","volume":"34","author":"AJ Scheen","year":"2011","journal-title":"Diabetes Care"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"718","DOI":"10.2164\/jandrol.111.013466","article-title":"Acceleration of sperm transit time and reduction of sperm reserves in the epididymis of rats exposed to sibutramine","volume":"32","author":"FF Bellentani","year":"2011","journal-title":"J Androl"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1530\/jrf.0.0540103","article-title":"Daily sperm production and epididymal sperm reserves of pubertal and adult rats","volume":"54","author":"GW Robb","year":"1978","journal-title":"J Reprod Fertil"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/j.1939-4640.1994.tb00458.x","article-title":"The ethane dimethanesulfonate-induced decrease in the fertilizing ability of cauda epididymal sperm is independent of the testis","volume":"15","author":"GR Klinefelter","year":"1994","journal-title":"J Androl"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1095\/biolreprod59.4.890","article-title":"Rat epididymal sperm quantity, quality, and transit time after guanethidine-induced sympathectomy","volume":"59","author":"WD Kempinas","year":"1998","journal-title":"Biol Reprod"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Klinefelter GR (2002) Actions of toxicants on the structure and function of the epididymis. In: Robaire B HB, editor. The Epididymis \u2013 From Molecules to Clinical Practice. New York: Kluwer Academic\/Plenum Publisher:. 353\u2013369.","DOI":"10.1007\/978-1-4615-0679-9_20"},{"key":"ref25","unstructured":"Kempinas WDG, Klinefelter GR. (2010) The Epididymis as a Target for Toxicants. Comprehensive Toxicology. 149\u2013166."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0272-0590(82)80059-6","article-title":"Use of animal models for detecting specific alterations in reproduction","volume":"2","author":"RP Amann","year":"1982","journal-title":"Fundam Appl Toxicol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0890-6238(96)00028-7","article-title":"Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. ILSI Risk Science Institute Expert Working Group on Sperm Evaluation","volume":"10","author":"J Seed","year":"1996","journal-title":"Reprod Toxicol"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Filler R (1993) Methods for evaluation of rats epididymal sperm morphology. In: R.E. Chapin JHH, editor. Male reproductive toxicology. California: Academic Press. 334\u2013343.","DOI":"10.1016\/B978-0-12-461207-5.50025-0"},{"key":"ref29","first-page":"91","article-title":"Methoxamine as a tool to assess the importance of intraneuronal uptake of l-norepinephrine in the cat&apos;s nictitating membrane","volume":"172","author":"U Trendelenburg","year":"1970","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0014-2999(68)90019-8","article-title":"The mechanism of supersensitivity to norepinephrine induced by cocaine in rat isolated vas deferens","volume":"4","author":"Y Kasuya","year":"1968","journal-title":"Eur J Pharmacol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1007\/BF00165750","article-title":"The mechanism of the 3H-noradrenaline releasing effect of various substrates of uptake1: multifactorial induction of outward transport","volume":"336","author":"A Langeloh","year":"1987","journal-title":"Naunyn-Schmiedeberg's archives of pharmacology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/BF00165751","article-title":"The mechanism of the 3H-noradrenaline releasing effect of various substrates of uptake1: role of monoamine oxidase and of vesicularly stored 3H-noradrenaline","volume":"336","author":"A Langeloh","year":"1987","journal-title":"Naunyn-Schmiedeberg's archives of pharmacology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1254\/jjp.90.281","article-title":"Method for simultaneous recording of the prostatic contractile and urethral pressure responses in anesthetized rats and the effects of tamsulosin","volume":"90","author":"H Kontani","year":"2002","journal-title":"Jpn J Pharmacol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1530\/jrf.0.0560643","article-title":"Antifertility effect of sympathomimetic drugs on male rats when applied locally to the vas deferens","volume":"56","author":"WD Ratnasooriya","year":"1979","journal-title":"J Reprod Fertil"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1530\/jrf.0.0580019","article-title":"Effect of local application of sympathomimetic drugs to the epididymis on fertility in rats","volume":"58","author":"WD Ratnasooriya","year":"1980","journal-title":"J Reprod Fertil"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1530\/jrf.0.0560633","article-title":"The effect of sympathomimetic drugs on contractility of the vas deferens in vitro and in vivo","volume":"56","author":"WD Ratnasooriya","year":"1979","journal-title":"J Reprod Fertil"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ejphar.2012.07.027","article-title":"Changes of cytosolic calcium and contractility of young rat vas deferens by acute treatment with amphetamine, fluoxetine or sibutramine","volume":"691","author":"NH Jurkiewicz","year":"2012","journal-title":"Eur J Pharmacol"},{"key":"ref38","unstructured":"Clegg ED, Perreault D, Klinefelter GR (2001) Assessment of male reproductive toxicity. In: Hayes AW, editor. Principles and Methods of Toxicology. Philadelphia: Taylor &amp; Francis. 1263\u20131300."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1590\/S0041-87812002000600008","article-title":"Prostate innervation and local anesthesia in prostate procedures","volume":"57","author":"AO Rodrigues","year":"2002","journal-title":"Rev Hosp Clin Fac Med Sao Paulo"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1002\/aja.1001540208","article-title":"Innervation of the ventral prostate of the rat","volume":"154","author":"A Vaalasti","year":"1979","journal-title":"Am J Anat"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"636181","DOI":"10.1155\/2011\/636181","article-title":"Present and future: pharmacologic treatment of obesity","volume":"2011","author":"M Glandt","year":"2011","journal-title":"J Obes"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s10286-005-0287-2","article-title":"Sibutramine and the sympathetic nervous system in obese humans","volume":"15","author":"WG Haynes","year":"2005","journal-title":"Clin Auton Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/S0022-5347(17)40043-7","article-title":"Ablation of the inferior mesenteric plexus in the rat: alteration of sperm storage in the epididymis and vas deferens","volume":"143","author":"KL Billups","year":"1990","journal-title":"J Urol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1530\/jrf.0.0710181","article-title":"Effects of cholinergic and adrenergic drugs on intraluminal pressures and contractility of the rat testis and epididymis in vivo","volume":"71","author":"C Pholpramool","year":"1984","journal-title":"J Reprod Fertil"},{"key":"ref45","first-page":"363","article-title":"Classification of calcium channels and the sites of action of drugs modifying channel function","volume":"44","author":"M Spedding","year":"1992","journal-title":"Pharmacol Rev"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1124\/jpet.106.117747","article-title":"Open channel block of A-type, kv4.3, and delayed rectifier K+ channels, Kv1.3 and Kv3.1, by sibutramine","volume":"321","author":"SE Kim","year":"2007","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1161\/01.ATV.21.1.28","article-title":"Potassium channel function in vascular disease","volume":"21","author":"CG Sobey","year":"2001","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1111\/j.1476-5381.1992.tb12791.x","article-title":"In vitro denervation of the rat vas deferens through hypothermic storage","volume":"107","author":"NH Jurkiewicz","year":"1992","journal-title":"British journal of pharmacology"},{"key":"ref49","unstructured":"Kenakin TP (1997) Competitive Antagonism. In: Kenakin TP, editor. Pharmacologic Analisys of Drug-Receptor Interaction. 3 ed: Lippincott-Raven. 331\u2013373."},{"key":"ref50","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/S0273-2300(03)00026-6","article-title":"Some aspects relating to the evaluation of the effects of chemicals on male fertility","volume":"37","author":"I Mangelsdorf","year":"2003","journal-title":"Regul Toxicol Pharmacol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0066091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T09:03:19Z","timestamp":1715504599000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0066091"}},"subtitle":[],"editor":[{"given":"Stefan","family":"Strack","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,6,12]]},"references-count":50,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,6,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0066091","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,12]]}}}