{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:36:53Z","timestamp":1773286613987,"version":"3.50.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2013,6,12]],"date-time":"2013-06-12T00:00:00Z","timestamp":1370995200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0066274","type":"journal-article","created":{"date-parts":[[2013,6,12]],"date-time":"2013-06-12T16:58:28Z","timestamp":1371056308000},"page":"e66274","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tightly Regulated and Homogeneous Transgene Expression in Human Adipose-Derived Mesenchymal Stem Cells by Lentivirus with Tet-Off System"],"prefix":"10.1371","volume":"8","author":[{"given":"Hiroyuki","family":"Moriyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Moriyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei","family":"Sawaragi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanayuki","family":"Okura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Ichinose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akifumi","family":"Matsuyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Hayakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1089\/ten.tec.2009.0208","article-title":"Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells","volume":"16","author":"H Okura","year":"2010","journal-title":"Tissue engineering Part C, Methods"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1089\/ten.tec.2009.0362","article-title":"Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model","volume":"16","author":"H Okura","year":"2010","journal-title":"Tissue engineering Part C, Methods"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s10047-009-0455-6","article-title":"Transdifferentiation of human adipose tissue-derived stromal cells into insulin-producing clusters","volume":"12","author":"H Okura","year":"2009","journal-title":"Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.expneurol.2004.01.027","article-title":"Characterization of neuronal\/glial differentiation of murine adipose-derived adult stromal cells","volume":"187","author":"KM Safford","year":"2004","journal-title":"Experimental neurology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1479-5876-8-63","article-title":"Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats","volume":"8","author":"S Leu","year":"2010","journal-title":"Journal of translational medicine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"675","DOI":"10.3109\/14653249.2010.549122","article-title":"Comparison of mesenchymal stem cells from adipose tissue and bone marrow for ischemic stroke therapy","volume":"13","author":"Y Ikegame","year":"2011","journal-title":"Cytotherapy"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.neuroscience.2011.02.038","article-title":"AMP-activated kinase mediates adipose stem cell-stimulated neuritogenesis of PC12 cells","volume":"181","author":"B Tan","year":"2011","journal-title":"Neuroscience"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Reid AJ, Sun M, Wiberg M, Downes S, Terenghi G, et al.. (2011) Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons from apoptosis. Neuroscience.","DOI":"10.1016\/j.neuroscience.2011.09.064"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1161\/01.CIR.0000121425.42966.F1","article-title":"Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells","volume":"109","author":"J Rehman","year":"2004","journal-title":"Circulation"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1524-475X.2009.00499.x","article-title":"Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF","volume":"17","author":"EY Lee","year":"2009","journal-title":"Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1471-2121-13-21","article-title":"Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling","volume":"13","author":"M Moriyama","year":"2012","journal-title":"BMC Cell Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1021\/mp200135e","article-title":"Genetically modified mesenchymal stem cells for improved islet transplantation","volume":"8","author":"H Wu","year":"2011","journal-title":"Mol Pharm"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1073\/pnas.251682798","article-title":"Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos","volume":"99","author":"A Pfeifer","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/S1525-0016(03)00186-2","article-title":"Treatment of myocardial ischemia with bone marrow-derived mesenchymal stem cells overexpressing hepatocyte growth factor","volume":"8","author":"HF Duan","year":"2003","journal-title":"Mol Ther"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/jgm.516","article-title":"Transcriptional silencing is associated with extensive methylation of the CMV promoter following adenoviral gene delivery to muscle","volume":"6","author":"AR Brooks","year":"2004","journal-title":"J Gene Med"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1089\/scd.2006.0088","article-title":"Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems","volume":"16","author":"S Kim","year":"2007","journal-title":"Stem Cells Dev"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1038\/embor.2009.201","article-title":"Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells","volume":"10","author":"D Meilinger","year":"2009","journal-title":"EMBO Rep"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e10611","DOI":"10.1371\/journal.pone.0010611","article-title":"Systematic comparison of constitutive promoters and the doxycycline-inducible promoter","volume":"5","author":"JY Qin","year":"2010","journal-title":"PLoS One"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/scrt53","article-title":"Lentiviral vector mediated modification of mesenchymal stem cells &amp; enhanced survival in an in vitro model of ischaemia","volume":"2","author":"L McGinley","year":"2011","journal-title":"Stem Cell Res Ther"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1002\/jgm.903","article-title":"Pharmacologic transgene control systems for gene therapy","volume":"8","author":"W Weber","year":"2006","journal-title":"J Gene Med"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Shi Q, Tian X, Zhao Y, Luo H, Tian Y, et al.. (2011) Anti-arthritic effects of FasL gene transferred intra-articularly by an inducible lentiviral vector containing improved tet-on system. Rheumatol Int.","DOI":"10.1007\/s00296-011-2002-z"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"498","DOI":"10.4161\/cc.8.3.7701","article-title":"Single-vector inducible lentiviral RNAi system for oncology target validation","volume":"8","author":"D Wiederschain","year":"2009","journal-title":"Cell Cycle"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.neures.2008.10.010","article-title":"High-level transgene expression in neurons by lentivirus with Tet-Off system","volume":"63","author":"H Hioki","year":"2009","journal-title":"Neurosci Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e23734","DOI":"10.1371\/journal.pone.0023734","article-title":"Development of an all-in-one lentiviral vector system based on the original TetR for the easy generation of Tet-ON cell lines","volume":"6","author":"K Benabdellah","year":"2011","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1089\/ten.tec.2010.0139","article-title":"Transplantation of human adipose tissue-derived multilineage progenitor cells reduces serum cholesterol in hyperlipidemic Watanabe rabbits","volume":"17","author":"H Okura","year":"2011","journal-title":"Tissue engineering Part C, Methods"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.bbrc.2011.07.035","article-title":"HMG-CoA reductase inhibitor augments the serum total cholesterol-lowering effect of human adipose tissue-derived multilineage progenitor cells in hyperlipidemic homozygous Watanabe rabbits","volume":"412","author":"A Saga","year":"2011","journal-title":"Biochemical and biophysical research communications"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1089\/ten.tec.2010.0139","article-title":"Transplantation of human adipose tissue-derived multilineage progenitor cells reduces serum cholesterol in hyperlipidemic Watanabe rabbits","volume":"17","author":"H Okura","year":"2011","journal-title":"Tissue Eng Part C Methods"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1006\/jmbi.2001.5015","article-title":"Promoter suppression in cultured mammalian cells can be blocked by the chicken beta-globin chromatin insulator 5\u2032HS4 and matrix\/scaffold attachment regions","volume":"312","author":"JF Villemure","year":"2001","journal-title":"J Mol Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"9381","DOI":"10.1093\/nar\/14.23.9381","article-title":"Comparison of promoter suppression in avian and murine retrovirus vectors","volume":"14","author":"M Emerman","year":"1986","journal-title":"Nucleic Acids Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1089\/ten.tea.2007.0241","article-title":"Nanobiomechanics of repair bone regenerated by genetically modified mesenchymal stem cells","volume":"14","author":"K Tai","year":"2008","journal-title":"Tissue Eng Part A"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1038\/mt.2009.67","article-title":"Enhancement of myogenic and muscle repair capacities of human adipose-derived stem cells with forced expression of MyoD","volume":"17","author":"S Goudenege","year":"2009","journal-title":"Mol Ther"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/jcp.20897","article-title":"Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells","volume":"211","author":"Y Li","year":"2007","journal-title":"J Cell Physiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1634\/stemcells.2007-0164","article-title":"Generation of insulin-producing cells from human bone marrow mesenchymal stem cells by genetic manipulation","volume":"25","author":"O Karnieli","year":"2007","journal-title":"Stem Cells"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1172\/JCI200420935","article-title":"Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation","volume":"113","author":"M Dezawa","year":"2004","journal-title":"J Clin Invest"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1093\/eurjhf\/hfp135","article-title":"Transplantation with survivin-engineered mesenchymal stem cells results in better prognosis in a rat model of myocardial infarction","volume":"11","author":"L Fan","year":"2009","journal-title":"Eur J Heart Fail"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1385\/ABAB:133:1:9","article-title":"Adenoviral vectors: a promising tool for gene therapy","volume":"133","author":"SS Ghosh","year":"2006","journal-title":"Appl Biochem Biotechnol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.ymthe.2005.04.015","article-title":"Efficient gene transfer into mouse embryonic stem cells with adenovirus vectors","volume":"12","author":"K Kawabata","year":"2005","journal-title":"Mol Ther"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1038\/gt.2009.46","article-title":"Independent and high-level dual-gene expression in adult stem-progenitor cells from a single lentiviral vector","volume":"16","author":"J Tian","year":"2009","journal-title":"Gene Ther"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.cell.2006.03.011","article-title":"Stem cells and cancer: two faces of eve","volume":"124","author":"MF Clarke","year":"2006","journal-title":"Cell"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1743-422X-3-14","article-title":"Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI","volume":"3","author":"D Chinnasamy","year":"2006","journal-title":"Virol J"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1089\/hum.2008.188","article-title":"Highly efficient multicistronic lentiviral vectors with peptide 2A sequences","volume":"20","author":"A Ibrahimi","year":"2009","journal-title":"Hum Gene Ther"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1101\/gr.095976.109","article-title":"The completion of the Mammalian Gene Collection (MGC)","volume":"19","author":"G Temple","year":"2009","journal-title":"Genome Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.bbrc.2007.09.007","article-title":"Blockade of invasion and metastasis of breast cancer cells via targeting CXCR4 with an artificial microRNA","volume":"363","author":"Z Liang","year":"2007","journal-title":"Biochem Biophys Res Commun"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0066274","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T03:58:47Z","timestamp":1563163127000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0066274"}},"subtitle":[],"editor":[{"given":"Niels Olsen Saraiva","family":"C\u00e2mara","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,6,12]]},"references-count":43,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,6,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0066274","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,12]]}}}