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maximum parsimony methods","volume":"28","author":"K Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"Jukes TH, Cantor CR (1969) Evolution of protein molecules. 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In: Fayer R, Xiao L, editors. <italic>Cryptosporidium<\/italic> and Cryptosporidiosis. 2nd ed. Boca Raton, FL: CRC Press and IWA Publishing. 119\u2013171."},{"key":"ref52","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2353\/jmoldx.2010.090035","article-title":"The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5\u2032 nuclease assay","volume":"12","author":"R Stadhouders","year":"2010","journal-title":"J Mol Diagn"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"536","DOI":"10.3201\/eid1703.100410","article-title":"Sporadic human cryptosporidiosis caused by <italic>Cryptosporidium cuniculus<\/italic>, United Kingdom, 2007\u20132008","volume":"17","author":"RM Chalmers","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.3201\/eid1411.080239","article-title":"Unusual <italic>Cryptosporidium<\/italic> genotypes in human cases of diarrhea","volume":"14","author":"G Robinson","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"e82","DOI":"10.1093\/nar\/gks203","article-title":"Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation","volume":"40","author":"AS Whale","year":"2012","journal-title":"Nucleic Acids Res"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0066562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T01:54:23Z","timestamp":1645840463000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0066562"}},"subtitle":[],"editor":[{"given":"Henk D. 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