{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:11:49Z","timestamp":1791090709522,"version":"4.1.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T00:00:00Z","timestamp":1372204800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0068097","type":"journal-article","created":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T23:24:46Z","timestamp":1372289086000},"page":"e68097","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Detection of Streptococcus pneumoniae from Different Types of Nasopharyngeal Swabs in Children"],"prefix":"10.1371","volume":"8","author":[{"given":"Felix S.","family":"Dube","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou","family":"Kaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Whittaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather J.","family":"Zar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Nicol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"408","DOI":"10.2471\/BLT.07.048769","article-title":"Epidemiology and etiology of childhood pneumonia","volume":"86","author":"I Rudan","year":"2008","journal-title":"Bull World Health Organ"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1016\/S0140-6736(10)60549-1","article-title":"Global, regional, and national causes of child mortality in 2008: a systematic analysis","volume":"375","author":"RE Black","year":"2010","journal-title":"Lancet"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S1473-3099(04)00938-7","article-title":"Streptococcus pneumoniae colonisation: the key to pneumococcal disease","volume":"4","author":"D Bogaert","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1111\/j.1365-3156.2009.02331.x","article-title":"Nasopharyngeal carriage of S. pneumoniae among young children in rural Nepal","volume":"14","author":"CL Coles","year":"2009","journal-title":"Trop Med Int Health"},{"key":"ref5","first-page":"1","article-title":"Molecular epidemiology of pneumococci obtained from Gambian children aged 2 \u2013 29 months with invasive pneumococcal disease during a trial of a 9-valent pneumococcal conjugate vaccine","volume":"10","author":"M Antonio","year":"2008","journal-title":"BMC Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1086\/506941","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae in Gambian villagers","volume":"43","author":"PC Hill","year":"2006","journal-title":"Clin Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1086\/313608","article-title":"Which pneumococcal serogroups cause the most invasive disease: implications for conjugate vaccine formulation and use, part I","volume":"30","author":"WP Hausdorff","year":"2000","journal-title":"Clin Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1378\/chest.09-0258","article-title":"Severity of pneumococcal pneumonia associated with genomic bacterial load","volume":"136","author":"J Rello","year":"2009","journal-title":"Chest"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1086\/322048","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae in Finnish children younger than 2 years old","volume":"184","author":"RK Syrj\u00e4nen","year":"2001","journal-title":"J Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1086\/516477","article-title":"Relationship between nasopharyngeal colonization and the development of otitis media in children","volume":"175","author":"H Faden","year":"1997","journal-title":"J Infect Dis"},{"key":"ref11","first-page":"191","article-title":"Qualification of high-recovery, flocked swabs as compared to traditional rayon swabs for microbiological environmental monitoring of surfaces","volume":"62","author":"G Dalmaso","year":"2008","journal-title":"PDA J Pharm Sci Technol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1128\/JCM.02055-05","article-title":"Comparison of flocked and rayon swabs for collection of respiratory epithelial cells from uninfected volunteers and symptomatic patients","volume":"44","author":"P Daley","year":"2006","journal-title":"J Clin Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s10096-010-1064-2","article-title":"Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs","volume":"30","author":"SS Hernes","year":"2011","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1186\/1471-2334-10-340","article-title":"Flocked nasal swab versus nasopharyngeal aspirate for detection of respiratory tract viruses in immunocompromised adults: a matched comparative study","volume":"10","author":"L Ohrmalm","year":"2010","journal-title":"BMC Infect Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jviromet.2012.06.009","article-title":"Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using real-time polymerase chain reaction","volume":"185","author":"C Debyle","year":"2012","journal-title":"J Virol Methods"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2414","DOI":"10.1128\/JCM.00369-08","article-title":"Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children","volume":"46","author":"A Abu-Diab","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1097\/INF.0b013e3182788fdd","article-title":"Pneumococcal carriage and invasive disease in children before introduction of the 13-valent conjugate vaccine: comparison with the era before 7-valent conjugate vaccine","volume":"32","author":"D Sharma","year":"2013","journal-title":"Pediatr Infect Dis J"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"4134","DOI":"10.1128\/JCM.02376-12","article-title":"Alternative sampling methods for detecting bacterial pathogens in children with upper respiratory tract infections","volume":"50","author":"MR van den Bergh","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3209","DOI":"10.1128\/JCM.00610-11","article-title":"Serotyping of Streptococcus pneumoniae isolates from nasopharyngeal samples: use of an algorithm combining microbiologic, serologic, and sequential multiplex PCR techniques","volume":"49","author":"K Miernyk","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"4242","DOI":"10.1128\/JCM.01425-10","article-title":"Better detection of Staphylococcus aureus nasal carriage by use of nylon flocked swabs","volume":"48","author":"P Verhoeven","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.diagmicrobio.2008.08.004","article-title":"Comparison of 3 swab transport systems for direct release and recovery of aerobic and anaerobic bacteria","volume":"62","author":"KG Van Horn","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1128\/JCM.02047-07","article-title":"Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability","volume":"46","author":"KG Van Horn","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1128\/JCM.01903-07","article-title":"Effect of swab composition and use of swabs versus swab-containing skim milk-tryptone-glucose-glycerol (STGG) on culture- or PCR-based detection of Streptococcus pneumoniae in simulated and clinical respiratory specimens in STGG transport medium","volume":"46","author":"LG Rubin","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1128\/JCM.39.3.1021-1024.2001","article-title":"Evaluation of a medium (STGG) for transport and optimal recovery of Streptococcus pneumoniae from nasopharyngeal secretions collected during field studies","volume":"39","author":"KL O\u2032Brien","year":"2001","journal-title":"J Clin Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1128\/JCM.01617-09","article-title":"Comparison of flocked and rayon swabs for detection of nasal carriage of Staphylococcus aureus among pathology staff members","volume":"48","author":"S De Silva","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Drake C, Barenfanger J, Lawhorn J, Verhulst S (2005) Comparison of Easy-Flow Copan Liquid Stuart&apos;s and Starplex swab transport systems for recovery of fastidious aerobic bacteria. J Clin Microbiol: 3\u20136.","DOI":"10.1128\/JCM.43.3.1301-1303.2005"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1128\/JCM.00224-11","article-title":"Detection of respiratory viruses by PCR assay of nasopharyngeal swabs stored in skim milk-tryptone-glucose-glycerol transport medium","volume":"49","author":"P Turner","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1097\/01.inf.0000049347.42983.77","article-title":"Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae","volume":"22","author":"KL O\u2032Brien","year":"2003","journal-title":"Pediatr Infect Dis J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.02498-06","article-title":"Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA","volume":"45","author":"G Carvalho Mda","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1099\/jmm.0.036574-0","article-title":"Accuracy of using the lytA gene to distinguish Streptococcus pneumoniae from related species","volume":"61","author":"T Greve","year":"2012","journal-title":"J Med Microbiol"},{"key":"ref31","unstructured":"CDC (<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.cdc.gov\/ncidod\/biotech\/files\/pcr-pneumo-carriage-March2010\" xlink:type=\"simple\">http:\/\/www.cdc.gov\/ncidod\/biotech\/files\/pcr-pneumo-carriage-March2010<\/ext-link>. Accessed 15 February 2013) Streptococcus pneumoniae carriage study protocol."},{"key":"ref32","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.jhin.2010.02.027","article-title":"New technique to take samples from environmental surfaces using flocked nylon swabs","volume":"75","author":"G Hedin","year":"2010","journal-title":"J Hosp Infect"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/cid\/cir859","article-title":"Use of a rapid test of pneumococcal colonization density to diagnose pneumococcal pneumonia","volume":"54","author":"WC Albrich","year":"2012","journal-title":"Clin Infect Dis"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1099\/jmm.0.044107-0","article-title":"Association between pneumococcal load and disease severity in adults with pneumonia","volume":"61","author":"AM Werno","year":"2012","journal-title":"J Med Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"5148","DOI":"10.1128\/AEM.00399-10","article-title":"Validation of a nylon-flocked-swab protocol for efficient recovery of bacterial spores from smooth and rough surfaces","volume":"76","author":"A Probst","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1128\/AEM.02037-10","article-title":"Recovery of bacillus spore contaminants from rough surfaces: a challenge to space mission cleanliness control","volume":"77","author":"A Probst","year":"2011","journal-title":"Appl Environ Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1159\/000329488","article-title":"A comparison of dacron versus Flocked nylon swabs for anal cytology specimen collection","volume":"55","author":"JC Gage","year":"2011","journal-title":"Acta Cytol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1111\/j.1556-4029.2011.02022.x","article-title":"A comparison of DNA collection and retrieval from two swab types (cotton and nylon flocked swab) when processed using three QIAGEN extraction methods","volume":"57","author":"RJ Brownlow","year":"2012","journal-title":"J Forensic Sci"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Esposito S, Molteni CG, Daleno C, Valzano A, Cesati L, <etal>et al<\/etal>.. (2010) Comparison of nasopharyngeal nylon flocked swabs with universal transport medium and rayon-bud swabs with a sponge reservoir of viral transport medium in the diagnosis of paediatric influenza. J Med Microbiol: 96\u201399.","DOI":"10.1099\/jmm.0.015305-0"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3340","DOI":"10.1128\/JCM.02235-09","article-title":"Development and evaluation of a flocked nasal midturbinate swab for self-collection in respiratory virus infection diagnostic testing","volume":"48","author":"M Smieja","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1128\/JCM.06384-11","article-title":"Sequetyping: Serotyping Streptococcus pneumoniae by a Single PCR Sequencing Strategy","volume":"50","author":"MH Leung","year":"2012","journal-title":"J Clin Microbiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0068097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:19:30Z","timestamp":1588969170000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0068097"}},"subtitle":[],"editor":[{"given":"Bernard","family":"Beall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,6,26]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,6,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0068097","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,26]]}}}