{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:02:35Z","timestamp":1760781755845},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T00:00:00Z","timestamp":1380758400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0074596","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T17:04:40Z","timestamp":1380819880000},"page":"e74596","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of the Mechanism of Inhibin \u03b1-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference"],"prefix":"10.1371","volume":"8","author":[{"given":"Li","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Canjie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasan","family":"Riaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liya","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aizhen","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liguo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,10,3]]},"reference":[{"key":"ref1","first-page":"724","article-title":"Activins, inhibins, and follistatins: from endocrinology to signaling. A paradigm for the new millennium","volume":"227","author":"C Welt","year":"2002","journal-title":"ExpBiol Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1073\/pnas.83.10.3091","article-title":"Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid","volume":"83","author":"RG Forage","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1210\/mend-1-8-561","article-title":"Rat inhibin: molecular cloning of alpha- and beta-subunit complementary deoxyribonucleic acids and expression in the ovary","volume":"1","author":"TK Woodruff","year":"1987","journal-title":"Mol Endocrinol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.anireprosci.2007.12.012","article-title":"Development and evaluation of a novel DNA vaccine expressing inhibin alpha (1-32) fragment for improving the fertility in rats and sheep","volume":"109","author":"L Han","year":"2008","journal-title":"Anim Reprod Sci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.theriogenology.2012.02.019","article-title":"Effect of a DNA vaccine harboring two copies of inhibin \u03b1 (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats","volume":"78","author":"SL Wang","year":"2012","journal-title":"Theriogenology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1507\/endocrj.43.403","article-title":"Inhibin alpha, beta A and beta B subunit messenger ribonucleic acid levels in cultured rat pituitary: studies by a quantitative RT-PCR","volume":"43","author":"R Demura","year":"1996","journal-title":"Endocr J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/humupd\/8.6.529","article-title":"Inhibins, activins and follistatin in reproduction","volume":"8","author":"DM De Kretser","year":"2002","journal-title":"Hum Reprod Update"},{"key":"ref8","first-page":"1075","article-title":"Regulable expression of inhibin A in wild-type and inhibin alpha null mice\u201d","volume":"14","author":"TM Pierson","year":"2000","journal-title":"Mol Endocrinol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1210\/endo-124-1-363","article-title":"Inhibitory effect of pure 31-kilodalton bovine inhibin on gonadotropin-releasing hormone (GnRH)-induced up-regulation of GnRH binding sites in cultured rat anterior pituitary cells","volume":"124","author":"QF Wang","year":"1989","journal-title":"Endocrinology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"5640","DOI":"10.1210\/en.2003-0670","article-title":"Betaglycan localization in the female rat pituitary: implications for the regulation of follicle-stimulating hormone by inhibin","volume":"144","author":"SC Chapman","year":"2003","journal-title":"Endocrinology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.mce.2003.09.019","article-title":"Regulation of FSHbeta and GnRH receptor gene expression in activin receptor II knockout male mice","volume":"212","author":"TR Kumar","year":"2003","journal-title":"Mol Cell Endocrinol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"16743","DOI":"10.1074\/jbc.M801045200","article-title":"Suppression of inhibin A biological activity by alterations in the binding site for betaglycan","volume":"283","author":"Y Makanji","year":"2008","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1126\/science.1064023","article-title":"Argonaute2, a link between genetic and biochemical analyses of RNAi","volume":"293","author":"SM Hammond","year":"2001","journal-title":"Science"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e25585","DOI":"10.1371\/journal.pone.0025585","article-title":"Action mechanism of inhibin \u03b1-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice","volume":"6","author":"K Cai","year":"2011","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1073\/pnas.82.4.1286","article-title":"Lithium induces corticotropin secretion and desensitization in cultured anterior pituitary cells","volume":"82","author":"M Zatz","year":"1985","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"W135","DOI":"10.1093\/nar\/gkh449","article-title":"Sfold web server for statistical folding and rational design of nucleic acids","volume":"32","author":"Y Ding","year":"2004","journal-title":"Nucleic Acids Research"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1101\/gad.862301","article-title":"RNA interference is mediated by 21- and 22-nucleotide RNAs","volume":"15","author":"SM Elbashir","year":"2001","journal-title":"Genes Dev"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1038\/13657","article-title":"Fluorescent proteins from nonbioluminescent Anthozoa species","volume":"17","author":"MV Matz","year":"1999","journal-title":"Nat Biotechnol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1095\/biolreprod.102.003673","article-title":"Inhibin a increases apoptosis in early ovarian antral follicles of diethylstilbestrol-treated rats","volume":"67","author":"AM Vitale","year":"2002","journal-title":"Biol Reprod"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S1673-8527(07)60126-X","article-title":"Effects of downregulation of inhibin alpha gene expression on apoptosis and proliferation of goose granulosa cells","volume":"34","author":"F Chen","year":"2007","journal-title":"J Genet Genomics"},{"key":"ref21","first-page":"51","article-title":"In vitro effects of inhibin on apoptosis and apoptosis related proteins in human ovarian granulosa cells","volume":"38","author":"R Denkova","year":"2004","journal-title":"EndocrRegul"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1038\/sj.cdd.4401067","article-title":"p53 deficiency fails to prevent increased programmed cell death in the Bcl-X(L)-deficient nervous system","volume":"9","author":"BJ Klocke","year":"2002","journal-title":"Cell Death Differ"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s00280-005-0050-3","article-title":"p53-independent G1 cell cycle arrest of human colon carcinoma cells HT-29 by sulforaphane is associated with induction of p21CIP1 and inhibition of expression of cyclin D1","volume":"57","author":"G Shen","year":"2006","journal-title":"Cancer Chemother Pharmacol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1210\/endo-127-5-2387","article-title":"Regulation of the synthetic rate of gonadotropin-releasing hormone receptors in rat pituitary cell cultures by inhibin","volume":"127","author":"TD Braden","year":"1990","journal-title":"Endocrinology"},{"key":"ref25","unstructured":"Gray P, Bilezikjian LM, Harrison C, Wiater E, Vale W (2004) Activins and inhibins: physiologic roles, signaling mechanisms and regulation. In Hormones and the Brain, pp 1\u201328."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"17011","DOI":"10.1074\/jbc.M601459200","article-title":"Identification of distinct inhibin and TGFb binding sites on betaglycan: functional separation of betaglycan co-receptor actions","volume":"281","author":"E Wiater","year":"2006","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1210\/endo.134.2.8299592","article-title":"Cells that express luteinizing hormone (LH) and follicle stimulating hormone (FSH) beta(\u03b2) subunit mRNAs during the estrous cycle: the major contributors contain LH, FSH and\/or growth hormone","volume":"134","author":"GV Childs","year":"1994","journal-title":"Endocrinology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"5811","DOI":"10.1210\/en.2008-0575","article-title":"Potent action of RFamide-related peptide-3 on pituitary gonadotropes indicative of a hypophysiotropic role in the negative regulation of gonadotropin secretion","volume":"149","author":"IJ Clarke","year":"2008","journal-title":"Endocrinology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"5549","DOI":"10.1210\/en.2009-0775","article-title":"Effect of RF-amide-related peptide-3 on luteinizing hormone and follicle-stimulating hormone synthesis and secretion in ovine pituitary gonadotropes","volume":"150","author":"IP Sari","year":"2009","journal-title":"Endocrinology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1111\/j.1365-2826.2005.01260.x","article-title":"Gonadotrophin inhibitory hormone depresses gonadotrophin alpha and follicle-stimulating hormone beta subunit expression in the pituitary of the domestic chicken","volume":"16","author":"NA Ciccone","year":"2004","journal-title":"J Neuroendocrinol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0303-7207(03)00235-1","article-title":"The gonadotropin releasing hormone (GnRH) receptor activating sequence (GRAS) is a composite regulatory element that interacts with multiple classes of transcription factors including Smads, AP-1 and a forkhead DNA binding protein","volume":"206","author":"BS Ellsworth","year":"2003","journal-title":"Mol Cell Endocrinol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1210\/en.2003-1047","article-title":"Regulation of luteinizing hormone-beta and follicle-stimulating hormone (FSH)-beta gene transcription by androgens: testosterone directly stimulates FSH-beta transcription independent from its role on follistatin gene expression","volume":"145","author":"LL Burger","year":"2004","journal-title":"Endocrinology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.mce.2009.09.003","article-title":"Hormones in synergy: regulation of the pituitary gonadotropin genes","volume":"314","author":"VG Thackray","year":"2010","journal-title":"Mol Cell Endocrinol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1146\/annurev.ph.57.030195.001251","article-title":"Inhibin, activin and the female reproductive axis","volume":"57","author":"TK Woodruff","year":"1995","journal-title":"Annu Rev Physiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2683","DOI":"10.1210\/endo-123-6-2683","article-title":"Effects of gonadal steroids on gonadotropin secretion in males: studies with perifused rat pituitary cells","volume":"123","author":"F Kotsuji","year":"1988","journal-title":"Endocrinology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1210\/endo.138.4.5090","article-title":"Differential effects of inhibin on gonadotropin stores and gonadotropin-releasing hormone binding to pituitary cells from cycling female rats","volume":"138","author":"GV Childs","year":"1997","journal-title":"Endocrinology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0303-7207(96)03802-6","article-title":"The mouse inhibin alpha-subunit promoter directs SV40 T-antigen to Leydig cells in transgenic mice","volume":"119","author":"K Kananen","year":"1996","journal-title":"Mol Cell Endocrinol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2332","DOI":"10.1210\/endo.132.6.8504739","article-title":"Recombinant human inhibin A and recombinant human activin A regulate pituitary and ovarian function in the adult female rat","volume":"132","author":"TK Woodruff","year":"1993","journal-title":"Endocrinology"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0074596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T22:46:15Z","timestamp":1540248375000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0074596"}},"subtitle":[],"editor":[{"given":"Rakesh N.","family":"Veedu","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,3]]},"references-count":38,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0074596","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,3]]}}}