{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T13:56:51Z","timestamp":1776952611409,"version":"3.51.4"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T00:00:00Z","timestamp":1380758400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0074919","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T17:03:41Z","timestamp":1380819821000},"page":"e74919","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Mitochondrial Ca2+-Handling in Fast Skeletal Muscle Fibers from Wild Type and Calsequestrin-Null Mice"],"prefix":"10.1371","volume":"8","author":[{"given":"Michele","family":"Scorzeto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Giacomello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana","family":"Toniolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Canato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bert","family":"Blaauw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Paolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feliciano","family":"Protasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Reggiani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ger J. M.","family":"Stienen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,10,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.1021\/bi2018909","article-title":"Role of mitochondrial Ca<sup>2+<\/sup> in the regulation of cellular energetics","volume":"51","author":"B Glancy","year":"2012","journal-title":"Biochemistry"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1126\/science.1081208","article-title":"BAX and BAK Regulation of Endoplasmic Reticulum Ca<sup>2+<\/sup>: A Control Point for Apoptosis","volume":"300","author":"L Scorrano","year":"2003","journal-title":"Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1038\/sj.cdd.4402147","article-title":"Mitochondrial Ca<sup>2+<\/sup> as a key regulator of cell life and death","volume":"14","author":"M Giacomello","year":"2007","journal-title":"Cell Death Differ"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.bbabio.2009.01.005","article-title":"Regulation of mitochondrial dehydrogenases by calcium ions","volume":"1787","author":"RM Denton","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1113\/jphysiol.1993.sp019937","article-title":"Energetics of fast- and slow-twitch muscles of the mouse","volume":"472","author":"CJ Barclay","year":"1993","journal-title":"J Physiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1006\/jmcc.2002.2082","article-title":"Cardiac energy metabolism homeostasis: role of cytosolic calcium","volume":"34","author":"RS Balaban","year":"2002","journal-title":"J Mol Cell Cardiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"32436","DOI":"10.1074\/jbc.M110.217711","article-title":"Mitochondrial calcium uptake regulates rapid calcium transients in skeletal muscle during excitation-contraction (E-C) coupling","volume":"286","author":"J Yi","year":"2011","journal-title":"J Biol Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1083\/jcb.200403102","article-title":"In vivo monitoring of Ca<sup>2+<\/sup> uptake into mitochondria of mouse skeletal muscle during contraction","volume":"166","author":"R Rudolf","year":"2004","journal-title":"J Cell Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1074\/jbc.M505024200","article-title":"Transfer and tunneling of Ca<sup>2+<\/sup> from sarcoplasmic reticulum to mitochondria in skeletal muscle","volume":"281","author":"VM Shkryl","year":"2006","journal-title":"J Biol Chem"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"C1128","DOI":"10.1152\/ajpcell.00194.2011","article-title":"Differential impact of mitochondrial positioning on mitochondrial Ca<sup>2+<\/sup> uptake and Ca<sup>2+<\/sup> spark suppression in skeletal muscle","volume":"301","author":"AE Rossi","year":"2011","journal-title":"Am J Physiol Cell Physiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nature10234","article-title":"Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter","volume":"476","author":"JM Baughman","year":"2011","journal-title":"Nature"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/nature10230","article-title":"A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter","volume":"476","author":"D De Stefani","year":"2011","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1073\/pnas.0908099107","article-title":"NCLX is an essential component of mitochondrial Na<sup>+<\/sup>\/Ca<sup>2+<\/sup> exchange","volume":"107","author":"R Palty","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1152\/physiol.00017.2007","article-title":"ER-mitochondria communication How privileged?","volume":"22","author":"C Franzini-Armstrong","year":"2007","journal-title":"Physiology (Bethesda)"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1152\/physrev.00004.2005","article-title":"Microdomains of intracellular Ca<sup>2+<\/sup>: molecular determinants and functional consequences","volume":"86","author":"R Rizzuto","year":"2006","journal-title":"Physiol Rev"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1091\/mbc.E08-07-0783","article-title":"Mitochondria are linked to calcium stores in striated muscle by developmentally regulated tethering structures","volume":"20","author":"S Boncompagni","year":"2009","journal-title":"Mol Biol Cell"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"22326","DOI":"10.1073\/pnas.1009168108","article-title":"Massive alterations of sarcoplasmic reticulum free calcium in skeletal muscle fibers lacking calsequestrin revealed by a genetically encoded probe","volume":"107","author":"M Canato","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1113\/jphysiol.2007.138024","article-title":"Reorganized stores and impaired calcium handling in skeletal muscle of mice lacking calsequestrin-1","volume":"583","author":"C Paolini","year":"2007","journal-title":"J Physiol"},{"key":"ref19","first-page":"C575","article-title":"Am J Physiol Cell Physiol","volume":"302","year":"2012"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1016\/j.bpj.2010.06.050","article-title":"Increased store-operated Ca<sup>2+<\/sup> entry in skeletal muscle with reduced calsequestrin-1 expression","volume":"99","author":"X Zhao","year":"2010","journal-title":"Biophys J"},{"key":"ref21","first-page":"634075","article-title":"Differential effect of calsequestrin ablation on structure and function of fast and slow skeletal muscle fibers","volume":"2011","author":"C Paolini","year":"2011","journal-title":"Biomed Biotechnol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.chembiol.2006.03.007","article-title":"Ca<sup>2+<\/sup> indicators based on computationally redesigned calmodulin-peptide pairs","volume":"13","author":"AE Palmer","year":"2006","journal-title":"Chem Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/jemt.20177","article-title":"Imaging and elasticity measurements of the sarcolemma of fully differentiated skeletal muscle fibers","volume":"67","author":"E Defranchi","year":"2005","journal-title":"Microsc Res Tech"},{"key":"ref24","first-page":"2510","article-title":"Casq2 deletion causes sarcoplasmic reticulum volume increase, premature Ca<sup>2+<\/sup> release, and catecholaminergic polymorphic ventricular tachycardia","volume":"116","author":"BC Knollmann","year":"2006","journal-title":"J Clin Invest"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ceca.2004.08.002","article-title":"Improved strategies for the delivery of GFP-based Ca<sup>2+<\/sup> sensors into the mitochondrial matrix","volume":"37","author":"L Filippin","year":"2005","journal-title":"Cell Calcium"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"DiFranco M, Quinonez M, Capote J, Vergara J (2009) DNA transfection of mammalian skeletal muscles using in vivo electroporation. J Vis Exp 32 pii: 1520.","DOI":"10.3791\/1520"},{"key":"ref27","first-page":"996","article-title":"Quantitative Evaluation of Cytoplasmic Structures in Electron Micrographs","volume":"14","author":"AV Loud","year":"1965","journal-title":"Lab Invest"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1085\/jgp.66.1.31","article-title":"Sizes of components in frog skeletal muscle measured by methods of stereology","volume":"66","author":"BA Mobley","year":"1975","journal-title":"J Gen Physiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1038\/nprot.2006.172","article-title":"Measuring calcium signaling using genetically targetable fluorescent indicators","volume":"1","author":"AE Palmer","year":"2006","journal-title":"Nat Protoc"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"16265","DOI":"10.1073\/pnas.1103015108","article-title":"Design and application of a class of sensors to monitor Ca<sup>2+<\/sup> dynamics in high Ca<sup>2+<\/sup> concentration cellular compartments","volume":"108","author":"S Tang","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1113\/jphysiol.1978.sp012273","article-title":"Calcium transients in isolated amphibian skeletal muscle fibers: detection with aequorin","volume":"277","author":"JR Blinks","year":"1978","journal-title":"J Physiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/j.bbabio.2008.12.011","article-title":"Characteristics and possible functions of mitochondrial Ca<sup>2+<\/sup> transport mechanisms","volume":"1787","author":"TE Gunter","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.yjmcc.2009.03.015","article-title":"Mitochondrial free calcium regulation during sarcoplasmic reticulum calcium release in rat cardiac myocytes","volume":"46","author":"TN Andrienko","year":"2009","journal-title":"J Mol Cell Cardiol"},{"key":"ref34","first-page":"73","article-title":"Quantitative ultrastructure of mammalian skeletal muscle","volume":"10","author":"BR Eisenberg","year":"1983","journal-title":"Section"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1113\/jphysiol.2003.050732","article-title":"Mitochondrial function in intact skeletal muscle fibers of creatine kinase deficient mice","volume":"552","author":"JD Bruton","year":"2003","journal-title":"J Physiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1113\/jphysiol.2008.163162","article-title":"Calsequestrin content and SERCA determine normal and maximal Ca<sup>2+<\/sup> storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat","volume":"587","author":"RM Murphy","year":"2009","journal-title":"J Physiol"},{"key":"ref37","first-page":"261","article-title":"Intracellular calcium movements during excitation\u2013contraction coupling in mammalian slow-twitch and fast-twitch muscle fibers J Gen Physiol","volume":"139","author":"SM Baylor","year":"2012"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1093\/hmg\/ddn355","article-title":"Increased mitochondrial Ca<sup>2+<\/sup> and decreased sarcoplasmic reticulum Ca<sup>2+<\/sup> in mitochondrial myopathy","volume":"18","author":"J Aydin","year":"2009","journal-title":"Hum Mol Genet"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1085\/jgp.200709827","article-title":"Simulation of Ca<sup>2+<\/sup> movements within the sarcomere of fast-twitch mouse fibers stimulated by action potentials","volume":"130","author":"SM Baylor","year":"2007","journal-title":"J Gen Physiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.yjmcc.2011.08.003","article-title":"Ca<sup>2+<\/sup> dynamics in the mitochondria \u2013 state of the art","volume":"51","author":"AC Chikando","year":"2011","journal-title":"J Mol Cell Cardiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.yjmcc.2008.12.011","article-title":"Mitochondrial Ca<sup>2+<\/sup> uptake: tortoise or hare?","volume":"46","author":"B O'Rourke","year":"2009","journal-title":"J Mol Cell Cardiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s11427-011-4203-9","article-title":"Distinctive characteristics and functions of multiple mitochondrial Ca<sup>2+<\/sup> influx mechanisms","volume":"54","author":"S Pan","year":"2011","journal-title":"Sci China Life Sci"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1074\/jbc.270.2.672","article-title":"The sodium-calcium antiport of heart mitochondria is not electroneutral","volume":"270","author":"DW Jung","year":"1995","journal-title":"J Biol Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s00424-007-0312-5","article-title":"The use of CalciumOrange-5N as a specific marker of mitochondrial Ca<sup>2+<\/sup> in mouse skeletal muscle fibers","volume":"455","author":"P Bola\u00f1os","year":"2008","journal-title":"Pflugers Arch"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3095","DOI":"10.1113\/jphysiol.2009.171967","article-title":"Calsequestrin-1: a new candidate gene for malignant hyperthermia and exertional\/environmental heat stroke","volume":"587","author":"F Protasi","year":"2009","journal-title":"J Physiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0074919","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T02:53:17Z","timestamp":1540003997000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0074919"}},"subtitle":[],"editor":[{"given":"Makoto","family":"Kanzaki","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,3]]},"references-count":45,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0074919","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,3]]}}}