{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:00:20Z","timestamp":1791129620725,"version":"4.1.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0075901","type":"journal-article","created":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T21:19:56Z","timestamp":1381958396000},"page":"e75901","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":91,"title":["Stiffness Dependent Separation of Cells in a Microfluidic Device"],"prefix":"10.1371","volume":"8","author":[{"given":"Gonghao","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Byler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin","family":"Henegar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Alexeev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Sulchek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"4589","DOI":"10.1073\/pnas.95.8.4589","article-title":"Detection and characterization of carcinoma cells in the blood","volume":"95","author":"E Racila","year":"1998","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1038\/nm0798-844","article-title":"Tissue microarrays for high-throughput molecular profiling of tumor specimens","volume":"4","author":"J Kononen","year":"1998","journal-title":"Nature Medicine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1039\/b604542a","article-title":"Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis","volume":"6","author":"N Pamme","year":"2006","journal-title":"Lab on a Chip"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s10544-006-0033-0","article-title":"Combined microfluidic-micromagnetic separation of living cells in continuous flow","volume":"8","author":"N Xia","year":"2006","journal-title":"Biomedical Microdevices"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1039\/B514539B","article-title":"Paramagnetic capture mode magnetophoretic microseparator for high efficiency blood cell separations","volume":"6","author":"KH Han","year":"2006","journal-title":"Lab on a Chip"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"8656","DOI":"10.1021\/ac8015938","article-title":"Multitarget Dielectrophoresis Activated Cell Sorter","volume":"80","author":"U Kim","year":"2008","journal-title":"Analytical Chemistry"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1002\/elps.200700607","article-title":"Dielectrophoretic separation of platelets from diluted whole blood in microfluidic channels","volume":"29","author":"MS Pommer","year":"2008","journal-title":"Electrophoresis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1021\/ac7020568","article-title":"An equilibrium method for continuous-flow cell sorting using dielectrophoresis","volume":"80","author":"MD Vahey","year":"2008","journal-title":"Analytical Chemistry"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.sna.2005.01.030","article-title":"A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process","volume":"121","author":"I Doh","year":"2005","journal-title":"Sensors and Actuators a-Physical"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/nbt1050","article-title":"Microfluidic sorting of mammalian cells by optical force switching","volume":"23","author":"MM Wang","year":"2005","journal-title":"Nature Biotechnology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"6365","DOI":"10.1021\/ac8007779","article-title":"Microfluidic-based cell sorting of Francisella tularensis infected macrophages using optical forces","volume":"80","author":"TD Perroud","year":"2008","journal-title":"Analytical Chemistry"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Ekpenyong AE, Whyte G, Chalut K, Pagliara S, Lautenschlager F, <etal>et al<\/etal>.. (2012) Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent. Plos One 7.","DOI":"10.1371\/journal.pone.0045237"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"6030","DOI":"10.1021\/ac9013572","article-title":"Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics","volume":"81","author":"A Lenshof","year":"2009","journal-title":"Analytical Chemistry"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"5117","DOI":"10.1021\/ac070444e","article-title":"Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation","volume":"79","author":"F Petersson","year":"2007","journal-title":"Analytical Chemistry"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1039\/b915113c","article-title":"Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW)","volume":"9","author":"JJ Shi","year":"2009","journal-title":"Lab on a Chip"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/15095","article-title":"A microfabricated fluorescence-activated cell sorter","volume":"17","author":"AY Fu","year":"1999","journal-title":"Nature Biotechnology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1039\/b705203k","article-title":"Continuous blood cell separation by hydrophoretic filtration","volume":"7","author":"S Choi","year":"2007","journal-title":"Lab on a Chip"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/nmeth.1452","article-title":"Using buoyant mass to measure the growth of single cells","volume":"7","author":"M Godin","year":"2010","journal-title":"Nature Methods"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1039\/c0lc00130a","article-title":"Adhesion based detection, sorting and enrichment of cells in microfluidic Lab-on-Chip devices","volume":"10","author":"TF Didar","year":"2010","journal-title":"Lab on a Chip"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/nnano.2007.388","article-title":"Nanomechanical analysis of cells from cancer patients","volume":"2","author":"SE Cross","year":"2007","journal-title":"Nature Nanotechnology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1039\/b802931h","article-title":"Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry","volume":"8","author":"MJ Rosenbluth","year":"2008","journal-title":"Lab on a Chip"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1039\/b614912j","article-title":"On-chip erythrocyte deformability test under optical pressure","volume":"7","author":"WG Lee","year":"2007","journal-title":"Lab on a Chip"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.actbio.2004.09.001","article-title":"Connections between single-cell biomechanics and human disease states: gastrointestinal cancer and malaria","volume":"1","author":"S Suresh","year":"2005","journal-title":"Acta Biomaterialia"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1529\/biophysj.104.045476","article-title":"Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence","volume":"88","author":"J Guck","year":"2005","journal-title":"Biophysical Journal"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Xu WW, Mezencev R, Kim B, Wang LJ, McDonald J, <etal>et al<\/etal>.. (2012) Cell Stiffness Is a Biomarker of the Metastatic Potential of Ovarian Cancer Cells. Plos One 7.","DOI":"10.1371\/journal.pone.0046609"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/cyto.a.20050","article-title":"Deformability-based flow cytometry","volume":"59A","author":"B Lincoln","year":"2004","journal-title":"Cytometry Part A"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1039\/c003873c","article-title":"Deformability based cell margination-A simple microfluidic design for malaria-infected erythrocyte separation","volume":"10","author":"HW Hou","year":"2010","journal-title":"Lab on a Chip"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1039\/c0lc00595a","article-title":"Deformability-based cell classification and enrichment using inertial microfluidics","volume":"11","author":"SC Hur","year":"2011","journal-title":"Lab on a Chip"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"7630","DOI":"10.1073\/pnas.1200107109","article-title":"Hydrodynamic stretching of single cells for large population mechanical phenotyping","volume":"109","author":"DR Gossett","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"18707","DOI":"10.1073\/pnas.1209893109","article-title":"Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells","volume":"109","author":"WJ Zhang","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"4006","DOI":"10.1039\/c2lc90100e","article-title":"Exploiting mechanical biomarkers in microfluidics","volume":"12","author":"XL Mao","year":"2012","journal-title":"Lab on a Chip"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"8289","DOI":"10.1016\/j.chroma.2009.05.036","article-title":"Isolation of tumor cells using size and deformation","volume":"1216","author":"H Mohamed","year":"2009","journal-title":"Journal of Chromatography A"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1039\/c2lc21083e","article-title":"Sorting cells by size, shape and deformability","volume":"12","author":"JP Beech","year":"2012","journal-title":"Lab on a Chip"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1242\/jcs.114.5.1025","article-title":"Effects of cytochalasin D and latrunculin B on mechanical properties of cells","volume":"114","author":"T Wakatsuki","year":"2001","journal-title":"Journal of Cell Science"},{"key":"ref35","first-page":"193","article-title":"Compaction of Cell Shape Occurs Before Decrease of Elasticity in CHO-K1 Cells Treated With Actin Cytoskeleton Disrupting Drug Cytochalasin D. Cell Motility and the Cytoskeleton","volume":"66","author":"C Schulze","year":"2009"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1039\/b908213a","article-title":"Designing microfluidic channel that separates elastic particles upon stiffness","volume":"5","author":"JP Arata","year":"2009","journal-title":"Soft Matter"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"051704","DOI":"10.1063\/1.3590264","article-title":"Hydrodynamic sorting of microparticles by size in ridged microchannels","volume":"23","author":"W Mao","year":"2011","journal-title":"Physics of Fluids"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.1529\/biophysj.105.067496","article-title":"Force microscopy of nonadherent cells: A comparison of leukemia cell deformability","volume":"90","author":"MJ Rosenbluth","year":"2006","journal-title":"Biophysical Journal"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.jbiomech.2009.07.037","article-title":"Stress relaxation microscopy: Imaging local stress in cells","volume":"43","author":"S Moreno-Flores","year":"2010","journal-title":"Journal of Biomechanics"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1126\/science.6362007","article-title":"Plasmodium-falciparum maturation abolishes phyciologic red-cell deformability","volume":"223","author":"HA Cranston","year":"1984","journal-title":"Science"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1172\/JCI114784","article-title":"Ascitic fluid from human ovarian-cancer patients contains growth-factors necessary for intraperitoneal growth of human ovarian adenocarcinoma cells","volume":"86","author":"GB Mills","year":"1990","journal-title":"Journal of Clinical Investigation"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.bbrc.2008.07.078","article-title":"AFM indentation study of breast cancer cells","volume":"374","author":"QS Li","year":"2008","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"Moreno-Flores S, Benitez R, Vivanco MD, Toca-Herrera JL (2010) Stress relaxation and creep on living cells with the atomic force microscope: a means to calculate elastic moduli and viscosities of cell components. Nanotechnology 21.","DOI":"10.1088\/0957-4484\/21\/44\/445101"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Succi S (2001) The lattice Boltzmann equation for fluid dynamics and beyond. Oxford: Oxford University Press. xvi, 288 pp.","DOI":"10.1093\/oso\/9780198503989.001.0001"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1023\/A:1010414013942","article-title":"Lattice-Boltzmann simulations of particle-fluid suspensions","volume":"104","author":"AJC Ladd","year":"2001","journal-title":"Journal of Statistical Physics"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1088\/0965-0393\/9\/6\/302","article-title":"A lattice spring model of heterogeneous materials with plasticity","volume":"9","author":"GA Buxton","year":"2001","journal-title":"Modelling and Simulation in Materials Science and Engineering"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1103\/PhysRevE.55.3271","article-title":"Deformation and failure in cellular materials","volume":"55","author":"AJC Ladd","year":"1997","journal-title":"Physical Review E"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"10244","DOI":"10.1021\/ma0516135","article-title":"Modeling the motion of microcapsules on compliant polymeric surfaces","volume":"38","author":"A Alexeev","year":"2005","journal-title":"Macromolecules"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"3452","DOI":"10.1063\/1.1399290","article-title":"Momentum transfer of a Boltzmann-lattice fluid with boundaries","volume":"13","author":"M Bouzidi","year":"2001","journal-title":"Physics of Fluids"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1021\/la062914q","article-title":"Patterned surfaces segregate compliant microcapsules","volume":"23","author":"A Alexeev","year":"2007","journal-title":"Langmuir"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"148103","DOI":"10.1103\/PhysRevLett.96.148103","article-title":"Designing compliant substrates to regulate the motion of vesicles","volume":"96","author":"A Alexeev","year":"2006","journal-title":"Physical Review Letters"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"034703","DOI":"10.1063\/1.2753150","article-title":"Modeling the interactions between compliant microcapsules and pillars in microchannels","volume":"127","author":"GD Zhu","year":"2007","journal-title":"Journal of Chemical Physics"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"Kilimnik A, Mao WB, Alexeev A (2011) Inertial migration of deformable capsules in channel flow. Physics of Fluids 23.","DOI":"10.1063\/1.3664402"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1016\/j.cma.2010.02.001","article-title":"Systematic coarse-graining of spectrin-level red blood cell models","volume":"199","author":"DA Fedosov","year":"2010","journal-title":"Computer Methods in Applied Mechanics and Engineering"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1146\/annurev.bioeng.2.1.189","article-title":"Cell mechanics: Mechanical response, cell adhesion, and molecular deformation","volume":"2","author":"C Zhu","year":"2000","journal-title":"Annual Review of Biomedical Engineering"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0075901","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T18:40:48Z","timestamp":1716057648000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0075901"}},"subtitle":[],"editor":[{"given":"Alexandre J.","family":"Kabla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,16]]},"references-count":55,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0075901","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,16]]}}}