{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T11:51:58Z","timestamp":1769082718236,"version":"3.49.0"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T00:00:00Z","timestamp":1380844800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0076661","type":"journal-article","created":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T21:09:19Z","timestamp":1380920959000},"page":"e76661","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Generation of Stable Lipid Raft Microdomains in the Enterocyte Brush Border by Selective Endocytic Removal of Non-Raft Membrane"],"prefix":"10.1371","volume":"8","author":[{"given":"E. Michael","family":"Danielsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gert H.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,10,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1083\/jcb.67.3.725","article-title":"Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells","volume":"67","author":"MS Mooseker","year":"1975","journal-title":"J Cell Biol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/0300-9084(88)90198-8","article-title":"Molecular organization of the intestinal brush border","volume":"70","author":"S Maroux","year":"1988","journal-title":"Biochimie"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev.cb.02.110186.001351","article-title":"Anchoring and biosynthesis of stalked brush border membrane proteins: glycosidases and peptidases of enterocytes and renal tubuli","volume":"2","author":"G Semenza","year":"1986","journal-title":"Annu Rev Cell Biol"},{"key":"ref4","first-page":"195","article-title":"Regulation of cytoskeletal structure and contractility in the brush border","volume":"95","author":"MS Mooseker","year":"1983","journal-title":"Ciba Found Symp"},{"key":"ref5","first-page":"50","article-title":"Structure of microvillar enzymes in different phases of their life cycles","volume":"95","author":"H Sjostrom","year":"1983","journal-title":"Ciba Found Symp"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/0005-2736(81)90245-5","article-title":"Microvillus membrane vesicles from pig small intestine. Purity and lipid composition","volume":"647","author":"K Christiansen","year":"1981","journal-title":"Biochim Biophys Acta"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/42408","article-title":"Functional rafts in cell membranes","volume":"387","author":"K Simons","year":"1997","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nrm2977","article-title":"Revitalizing membrane rafts: new tools and insights","volume":"11","author":"K Simons","year":"2010","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbamem.2003.09.005","article-title":"Lipid rafts in epithelial brush borders: atypical membrane microdomains with specialized functions","volume":"1617","author":"EM Danielsen","year":"2003","journal-title":"Biochim Biophys Acta"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"170","DOI":"10.3109\/09687688.2010.494625","article-title":"Domains of increased thickness in microvillar membranes of the small intestinal enterocyte","volume":"27","author":"AH Kunding","year":"2010","journal-title":"Mol Membr Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1023\/B:GLYC.0000014072.34840.04","article-title":"Introduction to galectins","volume":"19","author":"H Leffler","year":"2004","journal-title":"Glycoconj J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.ceb.2011.05.001","article-title":"Glycosylation, galectins and cellular signaling","volume":"23","author":"C Boscher","year":"2011","journal-title":"Curr Opin Cell Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/09687860500445604","article-title":"Lipid raft organization and function in brush borders of epithelial cells","volume":"23","author":"EM Danielsen","year":"2006","journal-title":"Mol Membr Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"5206","DOI":"10.1128\/IAI.00712-09","article-title":"Toxin-mediated effects on the innate mucosal defenses: implications for enteric vaccines","volume":"77","author":"GM Glenn","year":"2009","journal-title":"Infect Immun"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1038\/ncb2635","article-title":"Role of membrane traffic in the generation of epithelial cell asymmetry","volume":"14","author":"G Apodaca","year":"2012","journal-title":"Nat Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1242\/jcs.01217","article-title":"The subapical compartment: a traffic center in membrane polarity development","volume":"117","author":"D Hoekstra","year":"2004","journal-title":"J Cell Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/nrm2525","article-title":"Coordinated protein sorting, targeting and distribution in polarized cells","volume":"9","author":"I Mellman","year":"2008","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1042\/BC20090134","article-title":"Polarized traffic towards the cell surface: how to find the route","volume":"102","author":"M Carmosino","year":"2010","journal-title":"Biol Cell"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4253","DOI":"10.1242\/jcs.032615","article-title":"Apical trafficking in epithelial cells: signals, clusters and motors","volume":"122","author":"OA Weisz","year":"2009","journal-title":"J Cell Sci"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/cr.2012.64","article-title":"Polarized sorting and trafficking in epithelial cells","volume":"22","author":"X Cao","year":"2012","journal-title":"Cell Res"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1016\/S0960-9822(01)00446-8","article-title":"Apical membrane proteins are transported in distinct vesicular carriers","volume":"11","author":"R Jacob","year":"2001","journal-title":"Curr Biol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"159s","DOI":"10.1083\/jcb.99.1.159s","article-title":"Receptor-mediated transport of IgG","volume":"99","author":"R Rodewald","year":"1984","journal-title":"J Cell Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"G708","DOI":"10.1152\/ajpgi.00192.2009","article-title":"Endocytic trafficking from the small intestinal brush border probed with FM dye","volume":"297","author":"GH Hansen","year":"2009","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0167-4838(82)90400-9","article-title":"Further characterization of intestinal lactase\/phlorizin hydrolase","volume":"707","author":"H Skovbjerg","year":"1982","journal-title":"Biochim Biophys Acta"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1080\/00365517809104879","article-title":"Immunoelectrophoretic studies on human small intestinal brush border proteins. A qualitative study of the protein composition","volume":"38","author":"H Skovbjerg","year":"1978","journal-title":"Scand J Clin Lab Invest"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1042\/bj2020647","article-title":"Biosynthesis of intestinal microvillar proteins. Characterization of intestinal explants in organ culture and evidence for the existence of pro-forms of the microvillar enzymes","volume":"202","author":"EM Danielsen","year":"1982","journal-title":"Biochem J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1083\/jcb.120.6.1347","article-title":"Apical secretion of apolipoproteins from enterocytes","volume":"120","author":"EM Danielsen","year":"1993","journal-title":"J Cell Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1042\/bj1420575","article-title":"A rapid method for the preparation of microvilli from rabbit kidney","volume":"142","author":"AG Booth","year":"1974","journal-title":"Biochem J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1016\/S0264-410X(03)00336-0","article-title":"Transfer of antibody via mother's milk","volume":"21","author":"P Van de Perre","year":"2003","journal-title":"Vaccine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1083\/jcb.68.3.665","article-title":"Membrane flow during pinocytosis. A stereologic analysis","volume":"68","author":"RM Steinman","year":"1976","journal-title":"J Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1582-4934.2011.01378.x","article-title":"Lucifer yellow - an angel rather than the devil","volume":"16","author":"M Hanani","year":"2012","journal-title":"J Cell Mol Med"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF00234540","article-title":"Endocytosis in absorptive cells of cultured human small-intestinal tissue: horseradish peroxidase, lactoperoxidase, and ferritin as markers","volume":"216","author":"J Blok","year":"1981","journal-title":"Cell Tissue Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/s00418-008-0509-5","article-title":"Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells","volume":"130","author":"DL Marks","year":"2008","journal-title":"Histochem Cell Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1034\/j.1600-0854.2000.011101.x","article-title":"Membrane traffic in sphingolipid storage diseases","volume":"1","author":"RE Pagano","year":"2000","journal-title":"Traffic"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1021\/bi047959+","article-title":"Cholera Toxin Entry into Pig Enterocytes Occurs via a Lipid Raft- and Clathrin-Dependent Mechanism","volume":"44","author":"GH Hansen","year":"2005","journal-title":"Biochemistry"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"5795","DOI":"10.1073\/pnas.0631579100","article-title":"Resistance of cell membranes to different detergents","volume":"100","author":"S Schuck","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0092-8674(92)90189-J","article-title":"Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface","volume":"68","author":"DA Brown","year":"1992","journal-title":"Cell"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1016\/j.febslet.2009.12.043","article-title":"Membrane rafting: from apical sorting to phase segregation","volume":"584","author":"U Coskun","year":"2010","journal-title":"FEBS Lett"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1021\/bi00005a016","article-title":"Involvement of detergent-insoluble complexes in the intracellular transport of intestinal brush border enzymes","volume":"34","author":"EM Danielsen","year":"1995","journal-title":"Biochemistry"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1002\/ar.1091710302","article-title":"Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action","volume":"171","author":"JH Luft","year":"1971","journal-title":"Anat Rec"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1042\/bj20030235","article-title":"Deep-apical tubules: dynamic lipid-raft microdomains in the brush-border region of enterocytes","volume":"373","author":"GH Hansen","year":"2003","journal-title":"Biochem J"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev-cellbio-092910-154152","article-title":"Multivesicular body morphogenesis","volume":"28","author":"PI Hanson","year":"2012","journal-title":"Annu Rev Cell Dev Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.bbamem.2011.11.011","article-title":"Apolipoprotein A-1 (apoA-1) deposition in, and release from, the enterocyte brush border: a possible role in transintestinal cholesterol efflux (TICE)?","volume":"1818","author":"EM Danielsen","year":"2012","journal-title":"Biochim Biophys Acta"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev.iy.12.040194.000431","article-title":"Transepithelial transport of immunoglobulins","volume":"12","author":"KE Mostov","year":"1994","journal-title":"Annu Rev Immunol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1038\/nrm972","article-title":"Immunoglobulin transport across polarized epithelial cells","volume":"3","author":"R Rojas","year":"2002","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/0962-8924(92)90036-M","article-title":"Transepithelial transport and mucosal defence II: secretion of IgA","volume":"2","author":"JP Kraehenbuhl","year":"1992","journal-title":"Trends Cell Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1083\/jcb.5.3.363","article-title":"An electron microscopic study of the intestinal villus. I. The fasting animal","volume":"5","author":"SL Palay","year":"1959","journal-title":"J Biophys Biochem Cytol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00795-006-0346-3","article-title":"Molecular morphology of the digestive tract; macromolecules and food allergens are transferred intact across the intestinal absorptive cells during the neonatal-suckling period","volume":"40","author":"M Fujita","year":"2007","journal-title":"Med Mol Morphol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.tcb.2010.08.004","article-title":"Recycling endosomes in apical plasma membrane domain formation and epithelial cell polarity","volume":"20","author":"MR Golachowska","year":"2010","journal-title":"Trends Cell Biol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1172\/JCI118835","article-title":"Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A","volume":"98","author":"C Pothoulakis","year":"1996","journal-title":"J Clin Invest"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"a004721","DOI":"10.1101\/cshperspect.a004721","article-title":"Lipid-mediated endocytosis","volume":"3","author":"H Ewers","year":"2011","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1080\/09687860600682536","article-title":"Curvature-dependent lateral distribution of raft markers in the human erythrocyte membrane","volume":"23","author":"H Hagerstrand","year":"2006","journal-title":"Mol Membr Biol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"5622","DOI":"10.1073\/pnas.0811243106","article-title":"Curvature-driven lipid sorting needs proximity to a demixing point and is aided by proteins","volume":"106","author":"B Sorre","year":"2009","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0076661","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T04:27:40Z","timestamp":1540528060000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0076661"}},"subtitle":[],"editor":[{"given":"Manlio","family":"Vinciguerra","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,4]]},"references-count":53,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0076661","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,4]]}}}