{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:41:59Z","timestamp":1791164519852,"version":"4.1.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T00:00:00Z","timestamp":1381104000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0076743","type":"journal-article","created":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T21:00:27Z","timestamp":1381179627000},"page":"e76743","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":95,"title":["Conditional Gene Expression in Chlamydia trachomatis Using the Tet System"],"prefix":"10.1371","volume":"8","author":[{"given":"Jason","family":"Wickstrum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay R.","family":"Sammons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keasha N.","family":"Restivo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Scott","family":"Hefty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,10,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/S0966-842X(02)00011-2","article-title":"The cellular paradigm of chlamydial pathogenesis","volume":"11","author":"RS Stephens","year":"2003","journal-title":"Trends Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.femsre.2005.03.002","article-title":"The chlamydial developmental cycle","volume":"29","author":"YM Abdelrahman","year":"2005","journal-title":"FEMS Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"11430","DOI":"10.1073\/pnas.0703218104","article-title":"Mechanisms of host cell exit by the intracellular bacterium <italic>Chlamydia<\/italic>","volume":"104","author":"K Hybiske","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e1002258","DOI":"10.1371\/journal.ppat.1002258","article-title":"Development of a transformation system for <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic>: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector","volume":"7","author":"Y Wang","year":"2011","journal-title":"PLOS Pathog"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e57090","DOI":"10.1371\/journal.pone.0057090","article-title":"A <italic>C.<\/italic> <italic>trachomatis<\/italic> cloning vector and the generation of <italic>C.<\/italic> <italic>trachomatis<\/italic> strains expressing fluorescent proteins under the control of a <italic>C.<\/italic> <italic>trachomatis<\/italic> promoter","volume":"8","author":"H Agaisse","year":"2013","journal-title":"PLOS ONE"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.copbio.2006.09.001","article-title":"Genetic parts to program bacteria","volume":"17","author":"CA Voigt","year":"2006","journal-title":"Curr Opin Biotechnol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1751-7915.2007.00001.x","article-title":"The application of Tet repressor in prokaryotic gene regulation and expression","volume":"1","author":"R Bertram","year":"2008","journal-title":"J Microbiol Biotechnol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1146\/annurev.mi.48.100194.002021","article-title":"Mechanisms underlying expression of Tn10 encoded tetracycline resistance","volume":"48","author":"W Hillen","year":"1994","journal-title":"Annu Rev Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00203-006-0160-2","article-title":"Tetracycline-inducible gene expression in <italic>mycobacteria<\/italic> within an animal host using modified <italic>Streptomyces<\/italic> tcp830 regulatory elements","volume":"186","author":"SM Hernandez-Abanto","year":"2006","journal-title":"Arch Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.1128\/AEM.02825-08","article-title":"Development of a single-plasmid-based regulatable gene expression system for <italic>Borrelia<\/italic> <italic>burgdorferi<\/italic>","volume":"75","author":"CR Whetstine","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1093\/nar\/25.6.1203","article-title":"Independent and tight regulation of transcriptional units in <italic>Escherichia<\/italic> <italic>coli<\/italic> via the LacR\/O, the TetR\/O and AraC\/I1-I2 regulatory elements","volume":"25","author":"R Lutz","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.plasmid.2008.10.001","article-title":"An improved tetracycline-inducible expression vector for <italic>Staphylococcus<\/italic> <italic>aureus<\/italic>","volume":"61","author":"RM Corrigan","year":"2009","journal-title":"Plasmid"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1073\/pnas.89.12.5547","article-title":"Tight control of gene expression in mammalian cells by tetracycline-responsive promoters","volume":"89","author":"M Gossen","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"9302","DOI":"10.1073\/pnas.91.20.9302","article-title":"Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter","volume":"91","author":"PA Furth","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1126\/science.1063566","article-title":"Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA","volume":"293","author":"Y Ji","year":"2001","journal-title":"Science"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1128\/AAC.35.8.1591","article-title":"Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor","volume":"35","author":"J Degenkolb","year":"1991","journal-title":"Antimicrob Agents Chemother"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1128\/AAC.36.5.913","article-title":"Evidence that tetracycline analogs whose primary target is not the bacterial ribosome cause lysis of <italic>Escherichia<\/italic> <italic>coli<\/italic>","volume":"36","author":"B Oliva","year":"1992","journal-title":"Antimicrob Agents Chemother"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1093\/nar\/gkh200","article-title":"Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor","volume":"32","author":"A Kamionka","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/IAI.01305-12","article-title":"<italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes","volume":"81","author":"L Song","year":"2013","journal-title":"Infect Immun"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/2049-632X.12024","article-title":"Transformation of a plasmid-free, genital tract isolate of <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> with a plasmid vector carrying a deletion in CDS6 revealed that this gene regulates inclusion phenotype","volume":"67","author":"Y Wang","year":"2013","journal-title":"Pathog Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e59195","DOI":"10.1371\/journal.pone.0059195","article-title":"Genetic transformation of a clinical (genital tract), plasmid-free isolate of <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic>: engineering the plasmid as a cloning vector","volume":"8","author":"Y Wang","year":"2013","journal-title":"PLOS ONE"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0378-1119(94)90131-7","article-title":"A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments","volume":"141","author":"A Skerra","year":"1994","journal-title":"Gene"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/S0960-9822(02)00450-5","article-title":"Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer","volume":"6","author":"R Heim","year":"1996","journal-title":"Curr Biol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1128\/AAC.00427-12","article-title":"An automated image-based method for rapid analysis of <italic>Chlamydia<\/italic> infection as a tool for screening antichlamydial agents","volume":"56","author":"I Osaka","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1128\/AAC.00056-13","article-title":"Simple resazurin-based microplate assay for measuring <italic>Chlamydia<\/italic> infections","volume":"57","author":"I Osaka","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1042\/BJ20081949","article-title":"Far-red fluorescent tags for protein imaging in living tissues","volume":"418","author":"D Shcherbo","year":"2009","journal-title":"Biochem J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1099\/mic.0.29032-0","article-title":"The effect of penicillin on <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> DNA replication","volume":"152","author":"PR Lambden","year":"2006","journal-title":"Microbiology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0014-5793(99)01182-5","article-title":"Development of a quantitative gene expression assay for <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> identified temporal expression of sigma factors","volume":"458","author":"SA Mathews","year":"1999","journal-title":"FEBS Lett"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.bulm.2003.09.001","article-title":"Use of a quantitative gene expression assay based on micro-array techniques and a mathematical model for the investigation of chlamydial generation time","volume":"66","author":"DP Wilson","year":"2004","journal-title":"Bull Math Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JB.00511-13","article-title":"Characterization of <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> plasmid-encoded open reading frames","volume":"195","author":"S Gong","year":"2013","journal-title":"J Bacteriol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"5369","DOI":"10.1073\/pnas.0914365107","article-title":"Monomeric red fluorescent proteins with a large Stokes shift","volume":"107","author":"KD Piatkevich","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/nar\/gkg150","article-title":"Mutational analysis of the <italic>Chlamydia<\/italic> <italic>trachomatis<\/italic> <italic>dnaK<\/italic> promoter defines the optimal -35 promoter element","volume":"31","author":"CS Schaumburg","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"ref33","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers","volume":"132","author":"S Rozen","year":"2000","journal-title":"Methods Mol Biol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0076743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T05:33:52Z","timestamp":1646717632000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0076743"}},"subtitle":[],"editor":[{"given":"Daniel","family":"Rockey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,7]]},"references-count":33,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0076743","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,7]]}}}