{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:13:41Z","timestamp":1791108821331,"version":"4.1.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T00:00:00Z","timestamp":1383782400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0076989","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T22:11:18Z","timestamp":1383862278000},"page":"e76989","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Biological, Functional and Genetic Characterization of Bone Marrow-Derived Mesenchymal Stromal Cells from Pediatric Patients Affected by Acute Lymphoblastic Leukemia"],"prefix":"10.1371","volume":"8","author":[{"given":"Antonella","family":"Conforti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Biagini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Del Bufalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Sirleto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano","family":"Angioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Starc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Li Pira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Moretta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Proia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Contoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Genovese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Ciardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Antonietta","family":"Avanzini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittorio","family":"Rosti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Lo-Coco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Locatelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ester","family":"Bernardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,11,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1016\/S0140-6736(08)60457-2","article-title":"Acute lymphoblastic leukaemia","volume":"371","author":"CH Pui","year":"2008","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1056\/NEJMra052603","article-title":"Treatment of acute lymphoblastic leukemia","volume":"354","author":"CH Pui","year":"2006","journal-title":"N Engl J Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/978-1-59745-418-6_2","article-title":"Backtracking of leukemic clones to birth","volume":"538","author":"J Wiemels","year":"2009","journal-title":"Methods Mol Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nrc1164","article-title":"Origins of chromosome translocations in childhood leukaemia","volume":"3","author":"MF Greaves","year":"2003","journal-title":"Nat Rev Cancer"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/leu.2008.361","article-title":"Both expanded and uncultured mesenchymal stem cells from MDS patients are genomically abnormal, showing a specific genetic profile for the 5q- syndrome","volume":"23","author":"O Lopez-Villar","year":"2009","journal-title":"Leukemia"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1038\/sj.leu.2404621","article-title":"Bone marrow mesenchymal stem cells are abnormal in multiple myeloma","volume":"21","author":"J Corre","year":"2007","journal-title":"Leukemia"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.exphem.2006.10.012","article-title":"Chromosomal aberrations in bone marrow mesenchymal stroma cells from patients with myelodysplastic syndrome and acute myeloblastic leukemia","volume":"35","author":"O Blau","year":"2007","journal-title":"Exp Hematol"},{"key":"ref8","first-page":"230","article-title":"Heterotopic of bone marrow. Analyis of precursor cells for osteogenic and hematopoietic tissues","volume":"6","author":"AJ Friedenstein","year":"1968","journal-title":"Transplantation"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"MF Pittenger","year":"1999","journal-title":"Science"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nature00870","article-title":"Pluripotency of mesenchymal stem cells derived from adult marrow","volume":"418","author":"Y Jiang","year":"2002","journal-title":"Nature"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1038\/nature02041","article-title":"Identification of the haematopoietic stem cell niche and control of the niche size","volume":"425","author":"J Zhang","year":"2003","journal-title":"Nature"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nri1780","article-title":"Microenvironmental niches in the bone marrow required for B-cell development","volume":"6","author":"T Nagasawa","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1182\/blood.V99.10.3838","article-title":"Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli","volume":"99","author":"M Di Nicola","year":"2002","journal-title":"Blood"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/S0301-472X(01)00769-X","article-title":"Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo","volume":"30","author":"A Bartholomew","year":"2002","journal-title":"Exp Hematol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1182\/blood-2007-02-069716","article-title":"Immunomodulatory properties of mesenchymal stromal cells","volume":"110","author":"AJ Nauta","year":"2007","journal-title":"Blood"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1182\/blood-2007-04-087056","article-title":"Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation","volume":"110","author":"LM Ball","year":"2007","journal-title":"Blood"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1016\/S0140-6736(08)60690-X","article-title":"Mesenchymal stem cells for treatment of steroid-resistant severe, acute graft-versus-host disease: a phase II study","volume":"371","author":"K Le Blanc","year":"2008","journal-title":"Lancet"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1038\/leu.2011.384","article-title":"Mesenchymal stromal cells for the treatment of graft-versus-host disease: understanding the in vivo biological effect through patient immune monitoring","volume":"26","author":"E Dander","year":"2012","journal-title":"Leukemia"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1136\/gut.2010.215152","article-title":"Autologous bone marrow-derived mesenchymal stromal cell treatment for refractory luminal Crohn's disease: results of a phase I study","volume":"59","author":"M Duijvestein","year":"2010","journal-title":"Gut"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1136\/gut.2010.214841","article-title":"Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistuling Crohn's disease","volume":"60","author":"R Ciccocioppo","year":"2011","journal-title":"Gut"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1084\/jem.20091050","article-title":"Bone marrow mesenchymal stem cells from infants with MLL-AF4+ acute leukemia harbor and express the MLL-AF4 fusion gene","volume":"206","author":"P Menendez","year":"2009","journal-title":"J Exp Med"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.leukres.2005.12.010","article-title":"Assessment of bone marrow mesenchymal stem cell biological characteristics and support hematopoiesis function in patients with chronic myeloid leukemia","volume":"30","author":"Z Zhao","year":"2006","journal-title":"Leuk Res"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s00109-009-0583-8","article-title":"Leukemia-associated genetic aberration in mesenchymal stem cells of children with acute lymphoblastic leukemia","volume":"88","author":"S Shalapour","year":"2010","journal-title":"J Mol Med"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.exphem.2006.10.012","article-title":"Chromosomal aberrations in bone marrow mesenchymal stroma cells from patients with myelodisplastic syndrome and acute myeloblastic leukemia","volume":"35","author":"O Blau","year":"2007","journal-title":"Exp Hematol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2141.1976.tb03563.x","article-title":"Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group","volume":"33","author":"JM Bennett","year":"1976","journal-title":"Br J Haematol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1182\/blood-2009-03-209262","article-title":"The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes","volume":"114","author":"JW Vardiman","year":"2009","journal-title":"Blood"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1089\/scd.2008.0017","article-title":"Morpho-functional chracterization of human mesenchymal stem cells from umbilical cord blood for potential uses in regenerative medicine","volume":"18","author":"S Barachini","year":"2009","journal-title":"Stem Cell Dev"},{"key":"ref28","first-page":"845","article-title":"Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential","volume":"88","author":"PS in 't Anker","year":"2003","journal-title":"Haematologica"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"9142","DOI":"10.1158\/0008-5472.CAN-06-4690","article-title":"Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms","volume":"67","author":"ME Bernardo","year":"2007","journal-title":"Cancer Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/scd.2009.0062","article-title":"Bone marrow microenvironment in fanconi anemia: a prospective functional study in a cohort of fanconi anemia patients","volume":"19","author":"S Lecourt","year":"2010","journal-title":"Stem Cells Dev"},{"key":"ref31","first-page":"113","article-title":"RNA extraction by the guanidine thiocyanate procedure","volume":"2","author":"R McGookin","year":"1985","journal-title":"Methods of Molecular Biology"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1038\/sj.leu.2403135","article-title":"Standardization and quality control studies of \u2018real-time\u2019 quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia \u2013 A Europe Against Cancer Program","volume":"17","author":"J Gabert","year":"2003","journal-title":"Leukemia"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1080\/14653240701851332","article-title":"Properties and potential of bone marrow mesenchymal stromal cells from children with hematologic diseases","volume":"10","author":"H Dimitriou","year":"2008","journal-title":"Cytotherapy"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/B978-1-4831-9929-0.50010-3","article-title":"The vinca alkaloids","volume":"12","author":"N Neuss","year":"1964","journal-title":"Adv Chemother"},{"key":"ref35","first-page":"1286","article-title":"The mechanism of action of the folate antagonists in man","volume":"23","author":"JR Bertino","year":"1963","journal-title":"Cancer Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.bone.2003.07.005","article-title":"Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells","volume":"33","author":"K Stenderup","year":"2003","journal-title":"Bone"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1007\/s00277-012-1500-8","article-title":"Cytogenetic and molecular cytogenetic profile of bone marrow-derived mesenchymal stromal cells in chronic and acute lymphoproliferative disorders","volume":"91","author":"D Campioni","year":"2012","journal-title":"Ann Hematol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1182\/blood-2006-02-001479","article-title":"The stromal component of the marrow microenvironment is not derived from the malignant clone in MDS","volume":"108","author":"A Ramakrishnan","year":"2006","journal-title":"Blood"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1046\/j.1365-2141.2003.04679.x","article-title":"Role of the microenvironment in chronic lymphocytic leukemia","volume":"123","author":"F Caligaris-Cappio","year":"2003","journal-title":"Br J Haematol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1038\/sj.onc.1208405","article-title":"Mesenchymal cells generated from patients with myelodysplastic syndrome (MDS) are devoid of chromosomal clonal markers and support short and long-term hematopoiesis in vitro","volume":"24","author":"V Soenen-Cornu","year":"2005","journal-title":"Oncogene"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1182\/blood.V96.5.1926","article-title":"Stromal cells regulate survival of B-lineage leukemic cells during chemotherapy","volume":"96","author":"RE Mudry","year":"2000","journal-title":"Blood"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0076989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T14:43:21Z","timestamp":1596811401000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0076989"}},"subtitle":[],"editor":[{"given":"Pablo","family":"Menendez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,7]]},"references-count":41,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0076989","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,7]]}}}