{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:53:29Z","timestamp":1760298809891},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T00:00:00Z","timestamp":1381795200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0077161","type":"journal-article","created":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T23:15:50Z","timestamp":1381878950000},"page":"e77161","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of N-Glycosylation in Hepatocellular Carcinoma Patients\u2019 Serum with a Comparative Proteomic Approach"],"prefix":"10.1371","volume":"8","author":[{"given":"Yingnan","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruyi","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taotao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xizhong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,10,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/j.1365-2893.2009.01117.x","article-title":"Hepatitis B-related hepatocellular carcinoma: epidemiological characteristics and disease burden","volume":"16","author":"VT Nguyen","year":"2009","journal-title":"J Viral Hepat"},{"key":"ref2","first-page":"3","article-title":"Management of hepatitis B in China","volume":"122","author":"FM Lu","year":"2009","journal-title":"Chin Med J (Engl)"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3322\/caac.20006","article-title":"Cancer statistics, 2009","volume":"59","author":"A Jemal","year":"2009","journal-title":"CA Cancer J Clin"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1111\/j.1440-1746.2012.07173.x","article-title":"Des-gamma-carboxyl prothrombin is associated with tumor angiogenesis in hepatocellular carcinoma","volume":"27","author":"M Matsubara","year":"2012","journal-title":"J Gastroenterol Hepatol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1136\/gut.2010.214916","article-title":"Golgi protein 73 (GOLPH2) is a valuable serum marker for hepatocellular carcinoma","volume":"59","author":"Y Mao","year":"2010","journal-title":"Gut"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1007\/s10620-010-1184-7","article-title":"Serum markers of hepatocellular carcinoma","volume":"55","author":"G Malaguarnera","year":"2010","journal-title":"Dig Dis Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/ajg.2011.312","article-title":"Frequency of elevated hepatocellular carcinoma (HCC) biomarkers in patients with advanced hepatitis C","volume":"107","author":"RK Sterling","year":"2012","journal-title":"Am J Gastroenterol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"670","DOI":"10.2741\/3951","article-title":"Mechanisms of cancer-associated glycosylation changes","volume":"17","author":"F Dall'Olio","year":"2012","journal-title":"Front Biosci (Landmark Ed)"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1007\/s10439-011-0463-6","article-title":"Glycomechanics of the metastatic cascade: tumor cell-endothelial cell interactions in the circulation","volume":"40","author":"Y Geng","year":"2012","journal-title":"Ann Biomed Eng"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1042\/bj2090331","article-title":"Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes","volume":"209","author":"E Bause","year":"1983","journal-title":"Biochem J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1021\/cr000423j","article-title":"Protein N-glycosylation along the secretory pathway: relationship to organelle topography and function, protein quality control, and cell interactions","volume":"102","author":"J Roth","year":"2002","journal-title":"Chem Rev"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.1002\/cncr.25537","article-title":"TNM seventh edition: what&apos;s new, what&apos;s changed: communication from the International Union Against Cancer and The American Joint Committee on Cancer","volume":"116","author":"LH Sobin","year":"2010","journal-title":"Cancer"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"6560","DOI":"10.1021\/ac049062o","article-title":"Hydrophilic affinity isolation and MALDI multiple-stage tandem mass spectrometry of glycopeptides for glycoproteomics","volume":"76","author":"Y Wada","year":"2004","journal-title":"Anal Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1093\/glycob\/cwj019","article-title":"Differential analysis of site-specific glycans on plasma and cellular fibronectins: application of a hydrophilic affinity method for glycopeptide enrichment","volume":"15","author":"M Tajiri","year":"2005","journal-title":"Glycobiology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1021\/pr800826u","article-title":"Identification of N-glycosylation sites on secreted proteins of human hepatocellular carcinoma cells with a complementary proteomics approach","volume":"8","author":"J Cao","year":"2009","journal-title":"J Proteome Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1002\/hep.20720","article-title":"Albumin: biochemical properties and therapeutic potential","volume":"41","author":"GJ Quinlan","year":"2005","journal-title":"Hepatology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1002\/jbm.a.34491","article-title":"Depletion of albumin and immunoglobulin G from human serum using epitope-imprinted polymers as artificial antibodies","volume":"101","author":"HH Yang","year":"2013","journal-title":"J Biomed Mater Res A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1002\/elps.200500147","article-title":"Reduction of the concentration difference of proteins in biological liquids using a library of combinatorial ligands","volume":"26","author":"V Thulasiraman","year":"2005","journal-title":"Electrophoresis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/978-1-61779-573-2_13","article-title":"2D DIGE analysis of serum after fractionation by ProteoMiner beads","volume":"854","author":"C Liang","year":"2012","journal-title":"Methods Mol Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1074\/mcp.T600046-MCP200","article-title":"Shotgun glycopeptide capture approach coupled with mass spectrometry for comprehensive glycoproteomics","volume":"6","author":"B Sun","year":"2007","journal-title":"Mol Cell Proteomics"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1158\/0008-5472.CAN-03-2664","article-title":"Silencing expression of the clusterin\/apolipoprotein j gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability, and cell sensitization to genotoxic and oxidative stress","volume":"64","author":"IP Trougakos","year":"2004","journal-title":"Cancer Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jprot.2012.07.002","article-title":"2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer","volume":"77","author":"MA Penno","year":"2012","journal-title":"J Proteomics"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1038\/sj.onc.1207404","article-title":"Modulation of different clusterin isoforms in human colon tumorigenesis","volume":"23","author":"S Pucci","year":"2004","journal-title":"Oncogene"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1074\/jbc.M608162200","article-title":"Differential regulation of clusterin and its isoforms by androgens in prostate cells","volume":"282","author":"DR Cochrane","year":"2007","journal-title":"J Biol Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1021\/pr201041j","article-title":"Glycoprotein profiles of human breast cells demonstrate a clear clustering of normal\/benign versus malignant cell lines and basal versus luminal cell lines","volume":"11","author":"TY Yen","year":"2012","journal-title":"J Proteome Res"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.chroma.2012.07.066","article-title":"Multidimensional liquid chromatography platform for profiling alterations of clusterin N-glycosylation in the plasma of patients with renal cell carcinoma","volume":"1256","author":"F Tousi","year":"2012","journal-title":"J Chromatogr A"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1677\/ERC-09-0140","article-title":"The clusterin paradigm in prostate and breast carcinogenesis","volume":"17","author":"F Rizzi","year":"2010","journal-title":"Endocr Relat Cancer"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0065-230X(09)04003-2","article-title":"the shifting balance between CLU forms during tumor progression","volume":"104","author":"S Pucci","year":"2009","journal-title":"Adv Cancer Res"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1074\/mcp.M600143-MCP200","article-title":"Differential expression of serum clusterin isoforms in colorectal cancer","volume":"5","author":"AM Rodr\u00edguez-Pi\u00f1eiro","year":"2006","journal-title":"Mol Cell Proteomics"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"38079","DOI":"10.1074\/jbc.R300039200","article-title":"Inter-alpha-trypsin inhibitor, a covalent protein-glycosaminoglycan-protein complex","volume":"279","author":"L Zhuo","year":"2004","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"4292","DOI":"10.1002\/pmic.200700433","article-title":"Identification of putative serum glycoprotein biomarkers for human lung adenocarcinoma by multilectin affinity chromatography and LC-MS\/MS","volume":"7","author":"SH Heo","year":"2007","journal-title":"Proteomics"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/sj.onc.1210669","article-title":"The extracellular matrix protein ITIH5 is a novel prognostic marker in invasive node-negative breast cancer and its aberrant expression is caused by promoter hypermethylation","volume":"27","author":"J Veeck","year":"2008","journal-title":"Oncogene"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1756-8722-4-15","article-title":"Altered protein expression in serum from endometrial hyperplasia and carcinoma patients","volume":"4","author":"YS Wang","year":"2011","journal-title":"J Hematol Oncol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1471-2407-8-25","article-title":"Frequent expression loss of Inter-alpha-trypsin inhibitor heavy chain (ITIH) genes in multiple human solid tumors: a systematic expression analysis","volume":"8","author":"A Hamm","year":"2008","journal-title":"BMC Cancer"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1042\/bst0300971","article-title":"Factor H family proteins: on complement, microbes and human diseases","volume":"30","author":"PF Zipfel","year":"2002","journal-title":"Biochem Soc Trans"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1373\/clinchem.2004.042192","article-title":"Complement factor H as a marker for detection of bladder cancer","volume":"51","author":"ZZ Cheng","year":"2005","journal-title":"Clin Chem"},{"key":"ref37","first-page":"1775","article-title":"Serum proteomic analysis on metastasis-associated proteins of hepatocellular carcinoma","volume":"29","author":"BS Fu","year":"2009","journal-title":"Nan Fang Yi Ke Xue Xue Bao"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0077161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T19:15:07Z","timestamp":1540235707000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0077161"}},"subtitle":[],"editor":[{"given":"Andreas-Claudius","family":"Hoffmann","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,15]]},"references-count":37,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,15]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0077161","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,15]]}}}