{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:40:02Z","timestamp":1775151602304,"version":"3.50.1"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0077765","type":"journal-article","created":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T21:01:22Z","timestamp":1382043682000},"page":"e77765","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inter-Laboratory Assessment of a Prototype Multiplex Kit for Determination of Recent HIV-1 Infection"],"prefix":"10.1371","volume":"8","author":[{"given":"Kelly A.","family":"Curtis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew F.","family":"Longosz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Susan","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila","family":"Keating","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Heitman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Laeyendecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Michele","family":"Owen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,10,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1001\/jama.280.1.42","article-title":"New testing strategy to detect early HIV-1 infection for use in incidence estimates and for clinical and prevention purposes","volume":"280","author":"RS Janssen","year":"1998","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1093\/epirev\/mxq002","article-title":"Measuring the HIV\/AIDS epidemic: approaches and challenges","volume":"32","author":"R Brookmeyer","year":"2010","journal-title":"Epidemiol Rev"},{"key":"ref3","first-page":"1","article-title":"Estimating HIV Incidence in Populations Using Tests for Recent Infection: Issues, Challenges and the Way Forward","volume":"2","author":"TD Mastro","year":"2010","journal-title":"J HIV Aids Surveill Epidemiol"},{"key":"ref4","doi-asserted-by":"crossref","DOI":"10.12659\/MSM.881757","article-title":"Current and future assays for identifying recent HIV infections at the population level","volume":"17","author":"J Smole\u0144-Dzirba","year":"2011","journal-title":"Med Sci Monit"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1097\/00126334-200308150-00012","article-title":"Performance characteristics of a new less sensitive HIV-1 enzyme immunoassay for use in estimating HIV seroincidence","volume":"33","author":"D Kothe","year":"2003","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1097\/00126334-200307010-00009","article-title":"Development of a new less-sensitive enzyme immunoassay for detection of early HIV-1 infection","volume":"33","author":"BD Rawal","year":"2003","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1097\/00126334-200304010-00012","article-title":"Identifying recent HIV infections using the avidity index and an automated enzyme immunoassay","volume":"32","author":"B Suligoi","year":"2003","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref8","article-title":"Specificity of four laboratory approaches for cross-sectional HIV incidence determination: Analysis of samples from adults with known non-recent HIV infection from five African countries","author":"O Laeyendecker","year":"2012","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1089\/088922202753472874","article-title":"Quantitative detection of increasing HIV type 1 antibodies after seroconversion: a simple assay for detecting recent HIV infection and estimating incidence","volume":"18","author":"BS Parekh","year":"2002","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e33328","DOI":"10.1371\/journal.pone.0033328","article-title":"Detection of Recent HIV-1 Infection Using a New Limiting-Antigen Avidity Assay: Potential for HIV-1 Incidence Estimates and Avidity Maturation Studies","volume":"7","author":"YT Duong","year":"2012","journal-title":"PLOS ONE"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1089\/aid.2011.0037","article-title":"Development and characterization of a bead-based, multiplex assay for estimation of recent HIV type 1 infection","volume":"28","author":"KA Curtis","year":"2012","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1128\/JCM.43.9.4441-4447.2005","article-title":"Development and validation of an immunoassay for identification of recent human immunodeficiency virus type 1 infections and its use on dried serum spots","volume":"43","author":"F Barin","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1128\/JCM.42.6.2623-2628.2004","article-title":"Performance characteristics of the immunoglobulin G-capture BED-enzyme immunoassay, an assay to detect recent human immunodeficiency virus type 1 seroconversion","volume":"42","author":"T Dobbs","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1128\/JCM.01122-11","article-title":"A comprehensive evaluation of the proficiency testing program for the HIV-1 BED incidence assay","volume":"49","author":"T Dobbs","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1001\/jama.300.5.520","article-title":"Estimation of HIV incidence in the United States","volume":"300","author":"HI Hall","year":"2008","journal-title":"JAMA"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1089\/088922203769232511","article-title":"HIV type 1 incidence estimates by detection of recent infection from a cross-sectional sampling of injection drug users in Bangkok: use of the IgG capture BED enzyme immunoassay","volume":"19","author":"DJ Hu","year":"2003","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref17","first-page":"587","article-title":"Trends of HIV-1 seroincidence among HIV-1 sentinel surveillance groups in Cambodia, 1999-2002","volume":"39","author":"V Saphonn","year":"2005","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1001\/jama.300.5.540","article-title":"Risk factors for recent HIV infection in Uganda","volume":"300","author":"J Mermin","year":"2008","journal-title":"JAMA"},{"key":"ref19","first-page":"40","article-title":"UNAIDS Reference Group on estimates, modelling and projections--statement on the use of the BED assay for the estimation of HIV-1 incidence for surveillance or epidemic monitoring","volume":"81","year":"2006","journal-title":"Wkly Epidemiol Rec"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1097\/QAI.0b013e318050d277","article-title":"Do tests devised to detect recent HIV-1 infection provide reliable estimates of incidence in Africa?","volume":"45","author":"C Sakarovitch","year":"2007","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref21","first-page":"476","article-title":"Overestimation of the South African HIV incidence using the BED IgG assay?","volume":"97","author":"D Westreich","year":"2007","journal-title":"S Afr Med J"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1089\/aid.2011.0258","article-title":"Factors Associated with Incorrect Identification of Recent HIV Infection Using the BED Capture Immunoassay","volume":"28","author":"O Laeyendecker","year":"2012","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1089\/aid.2009.0133","article-title":"Development of two avidity-based assays to detect recent HIV type 1 seroconversion using a multisubtype gp41 recombinant protein","volume":"26","author":"X Wei","year":"2010","journal-title":"AIDS Res Hum Retrovir"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e18402","DOI":"10.1371\/journal.pone.0018402","article-title":"Dual testing algorithm of BED-CEIA and AxSYM Avidity Index assays performs best in identifying recent HIV infection in a sample of Rwandan sex workers","volume":"6","author":"SL Braunstein","year":"2011","journal-title":"PLOS ONE"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1093\/infdis\/jis659","article-title":"HIV Incidence Determination in the United States: A Multiassay Approach","volume":"207","author":"O Laeyendecker","year":"2013","journal-title":"J Infect Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"e64201","DOI":"10.1371\/journal.pone.0064201","article-title":"Evaluation of a multiplex assay for estimation of HIV-1 incidence","volume":"8","author":"KA Curtis","year":"2013","journal-title":"PLOS ONE"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1086\/317658","article-title":"Early human immunodeficiency virus (HIV) infection in the HIV Network for Prevention Trials Vaccine Preparedness Cohort: risk behaviors, symptoms, and early plasma and genital tract virus load","volume":"183","author":"CL Celum","year":"2001","journal-title":"J Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1093\/aje\/153.7.619","article-title":"Are US populations appropriate for trials of human immunodeficiency virus vaccine? The HIVNET Vaccine Preparedness Study","volume":"153","author":"GR Seage 3rd","year":"2001","journal-title":"Am J Epidemiol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0077765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T07:18:42Z","timestamp":1540019922000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0077765"}},"subtitle":[],"editor":[{"given":"Barbara","family":"Ensoli","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,10,17]]},"references-count":28,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0077765","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,17]]}}}