{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T01:03:42Z","timestamp":1783040622510,"version":"3.54.6"},"reference-count":98,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T00:00:00Z","timestamp":1384732800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0078877","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T03:36:35Z","timestamp":1384832195000},"page":"e78877","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation"],"prefix":"10.1371","volume":"8","author":[{"given":"Jayme L.","family":"Dahlin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rondedrick","family":"Sinville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Solberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhashree","family":"Francis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Strasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek J.","family":"Hook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Walters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,11,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.cell.2007.02.005","article-title":"Chromatin modifications and their function","volume":"128","author":"T Kouzarides","year":"2007","journal-title":"Cell"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.cell.2007.01.015","article-title":"The role of chromatin during transcription","volume":"128","author":"B Li","year":"2007","journal-title":"Cell"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1038\/nature03714","article-title":"A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response","volume":"436","author":"H Masumoto","year":"2005","journal-title":"Nature"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.molcel.2007.02.006","article-title":"Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes","volume":"25","author":"T Tsubota","year":"2007","journal-title":"Mol Cell"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1126\/science.1133234","article-title":"Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication","volume":"315","author":"J Han","year":"2007","journal-title":"Science"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1126\/science.1135862","article-title":"Yeast Rtt109 promotes genome stability by acetylating histone H3 on lysine 56","volume":"315","author":"R Driscoll","year":"2007","journal-title":"Science"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.cell.2008.06.035","article-title":"Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair","volume":"134","author":"CC Chen","year":"2008","journal-title":"Cell"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.cell.2008.06.018","article-title":"Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly","volume":"134","author":"Q Li","year":"2008","journal-title":"Cell"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/nature07861","article-title":"CBP\/p300-mediated acetylation of histone H3 on lysine 56","volume":"459","author":"C Das","year":"2009","journal-title":"Nature"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1038\/emboj.2009.119","article-title":"Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells","volume":"28","author":"J Tjeertes","year":"2009","journal-title":"EMBO J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/nsmb.1448","article-title":"Fungal Rtt109 histone acetyltransferase is an unexpected structural homolog of metazoan p300\/CBP","volume":"15","author":"Y Tang","year":"2008","journal-title":"Nat Struct Mol Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.sbi.2008.09.004","article-title":"Structure and chemistry of the p300\/CBP and Rtt109 histone acetyltransferases: implications for histone acetyltransferase evolution and function","volume":"18","author":"L Wang","year":"2008","journal-title":"Curr Opin Struct Biol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"12236","DOI":"10.1073\/pnas.0805813105","article-title":"Molecular basis for the autoregulation of the protein acetyl transferase Rtt109","volume":"105","author":"P Stavropoulos","year":"2008","journal-title":"P Natl Acad Sci USA"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"24694","DOI":"10.1074\/jbc.M111.251579","article-title":"Autoacetylation of the histone acetyltransferase Rtt109","volume":"286","author":"BN Albaugh","year":"2011","journal-title":"J Biol Chem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1038\/nsmb.1459","article-title":"Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75","volume":"15","author":"CE Berndsen","year":"2008","journal-title":"Nat Struct Mol Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"28587","DOI":"10.1074\/jbc.M702496200","article-title":"Acetylation of lysine 56 of histone H3 catalyzed by Rtt109 and regulated by Asf1 is required for replisome integrity","volume":"282","author":"J Han","year":"2007","journal-title":"J Biol Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"14158","DOI":"10.1074\/jbc.M700611200","article-title":"The Rtt109-Vps75 histone acetyltransferase complex acetylates non-nucleosomal histone H3","volume":"282","author":"J Han","year":"2007","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/j.str.2008.07.006","article-title":"Structural insights into histone H3 lysine 56 acetylation by Rtt109","volume":"16","author":"C Lin","year":"2008","journal-title":"Structure"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.str.2010.12.012","article-title":"Structure of the Rtt109-AcCoA\/Vps75 complex and implications for chaperone-mediated histone acetylation","volume":"19","author":"Y Tang","year":"2011","journal-title":"Structure"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e1001354","DOI":"10.1371\/journal.pgen.1001354","article-title":"H3 lysine 4 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation","volume":"7","author":"B Guillemette","year":"2011","journal-title":"PLoS Genet"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"4342","DOI":"10.1128\/MCB.00182-08","article-title":"Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109","volume":"28","author":"J Fillingham","year":"2008","journal-title":"Mol Cell Biol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.molcel.2010.01.020","article-title":"A role for Gcn5 in replication-coupled nucleosome assembly","volume":"37","author":"RJ Burgess","year":"2010","journal-title":"Mol Cell"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jprot.2012.09.026","article-title":"Chaperone-mediated acetylation of histones by Rtt109 identified by quantitative proteomics","volume":"81","author":"N Abshiru","year":"2012","journal-title":"J Proteomics"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4358","DOI":"10.1182\/blood-2002-05-1496","article-title":"Invasive aspergillosis in allogeneic stem cell transplant recipients: changes in epidemiology and risk factors","volume":"100","author":"KA Marr","year":"2002","journal-title":"Blood"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1086\/339202","article-title":"Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients","volume":"34","author":"KA Marr","year":"2002","journal-title":"Clin Infect Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1182\/blood-2002-05-1356","article-title":"Evidence-based assessment of primary antifungal prophylaxis in patients with hematologic malignancies","volume":"101","author":"OA Cornely","year":"2003","journal-title":"Blood"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/S0966-842X(03)00117-3","article-title":"Antifungal agents: mechanisms of action","volume":"11","author":"FC Odds","year":"2003","journal-title":"Trends Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/S0140-6736(02)08162-X","article-title":"Antifungal drug resistance of pathogenic fungi","volume":"359","author":"D Kontoyiannis","year":"2002","journal-title":"Lancet"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1469-0691.2008.01980.x","article-title":"Contribution of new diagnostic approaches to antifungal treatment plans in high-risk haematology patients","volume":"14","author":"H Einsele","year":"2008","journal-title":"Clin Microbiol Infect"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1097\/00001432-200312000-00002","article-title":"Impact of diagnostic markers on early antifungal therapy","volume":"16","author":"B Jones","year":"2003","journal-title":"Curr Opin Infect Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12026-012-8265-y","article-title":"Complexity and dynamics of host-fungal interactions","volume":"53","author":"F Cottier","year":"2012","journal-title":"Immunol Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.mib.2011.09.007","article-title":"Mechanisms of immune evasion in fungal pathogens","volume":"14","author":"J Collette","year":"2011","journal-title":"Curr Opin Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"6375","DOI":"10.1021\/bi100381y","article-title":"Kinetic mechanism of the Rtt109-Vps75 histone acetyltransferase-chaperone complex","volume":"49","author":"BN Albaugh","year":"2010","journal-title":"Biochemistry"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1038\/nm.2175","article-title":"Modulation of histone H3 lysine 56 acetylation as an antifungal therapeutic strategy","volume":"16","author":"H Wurtele","year":"2010","journal-title":"Nat Med"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1073\/pnas.0912427107","article-title":"Histone acetyltransferase Rtt109 is required for <italic>Candida albicans<\/italic> pathogenesis","volume":"107","author":"JLd Rosa","year":"2010","journal-title":"P Natl Acad Sci USA"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1165\/rcmb.2009-0443OC","article-title":"<italic>Pneumocystis carinii<\/italic> expresses an active Rtt109 histone acetyltransferase","volume":"44","author":"TJ Kottom","year":"2011","journal-title":"Am J Resp Cell Mol Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1128\/IAI.01173-12","article-title":"Characterization of the <italic>Pneumocystis carinii<\/italic> histone acetyltranferase chaperone proteins PcAsf1 and PcVps75","volume":"81","author":"J Pupaibool","year":"2013","journal-title":"Infect Immun"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1016\/j.bmcl.2013.03.112","article-title":"A small molecule inhibitor of fungal histone acetyltransferase Rtt109","volume":"23","author":"JLd Rosa","year":"2013","journal-title":"Bioorg Med Chem Lett"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1177\/29.2.7019305","article-title":"New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore","volume":"29","author":"TO Sippel","year":"1981","journal-title":"J Histochem Cytochem"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1006\/abio.2000.4855","article-title":"Application of a fluorescent histone acetyltransferase assay to probe the substrate specificity of the human p300\/CBP-associated factor","volume":"287","author":"RC Trievel","year":"2000","journal-title":"Anal Biochem"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1089\/adt.2007.105","article-title":"A fluorescence-based thiol quantification assay for ultra-high-throughput screening for inhibitors of coenzyme A production","volume":"6","author":"CC Chung","year":"2008","journal-title":"Assay Drug Dev Technol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1039\/c1mb05230f","article-title":"Formulating a fluorogenic assay to evaluate <italic>S<\/italic>-adenosyl-L-methionine analogues as protein methyltransferase cofactors","volume":"7","author":"R Wang","year":"2011","journal-title":"Mol Biosyst"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1177\/1087057111417712","article-title":"A continuous, fluorescent, high-throughput assay for human dimethylarginine dimethylaminohydrolase-1","volume":"16","author":"T Linsky","year":"2011","journal-title":"J Biomol Screen"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.4155\/fmc.11.84","article-title":"The University of Kansas High-Throughput Screening Laboratory. Part II: enabling collaborative drug-discovery partnerships through cutting-edge screening technology","volume":"3","author":"PR McDonald","year":"2011","journal-title":"Future Med Chem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"8749","DOI":"10.1074\/jbc.M111212200","article-title":"Biochemical analysis of chromatin containing recombinant <italic>Drosophila<\/italic> core histones","volume":"277","author":"M Levenstein","year":"2002","journal-title":"J Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"15625","DOI":"10.1074\/jbc.C111.220715","article-title":"Structure and histone binding properties of the Vps75-Rtt109 chaperone-lysine acetyltransferase complex","volume":"286","author":"D Su","year":"2011","journal-title":"J Biol Chem"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"M Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nchembio0807-438","article-title":"Reporting data from high-throughput screening of small-molecule libraries","volume":"3","author":"J Inglese","year":"2007","journal-title":"Nat Chem Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/0003-2697(85)90390-2","article-title":"The reaction of horseradish peroxidase with hydroperoxides derived from Triton X-100","volume":"146","author":"T Miki","year":"1985","journal-title":"Anal Biochem"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays","volume":"4","author":"J Zhang","year":"1999","journal-title":"J Biomol Screen"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1021\/jm901137j","article-title":"New substructure filters for removal of pan assay interference compounds (PAINS) from screening libraries and for their exclusion in bioassays","volume":"53","author":"JB Baell","year":"2010","journal-title":"J Med Chem"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.ab.2010.11.004","article-title":"Application of a high-throughput fluorescent acetyltransferase assay to identify inhibitors of homocitrate synthase","volume":"410","author":"S Bulfer","year":"2011","journal-title":"Anal Biochem"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1089\/adt.2009.0247","article-title":"Profiling the NIH Small Molecule Repository for compounds that generate H<sub>2<\/sub>O<sub>2<\/sub> by redox cycling in reducing environments","volume":"8","author":"KM Soares","year":"2010","journal-title":"Assay Drug Dev Technol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"e27197","DOI":"10.1371\/journal.pone.0027197","article-title":"Redox-based inactivation of cysteine cathepsins by compounds containing the 4-aminophenol moiety","volume":"6","author":"B Mirkovi\u0107","year":"2011","journal-title":"PLoS ONE"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/nprot.2006.77","article-title":"A detergent-based assay for the detection of promiscuous inhibitors","volume":"1","author":"BY Feng","year":"2006","journal-title":"Nat Protoc"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"4265","DOI":"10.1021\/jm030266r","article-title":"A specific mechanism of nonspecific inhibition","volume":"46","author":"S McGovern","year":"2003","journal-title":"J Med Chem"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.ymeth.2005.03.002","article-title":"Assays for mechanistic investigations of protein\/histone acetyltransferases","volume":"36","author":"CE Berndsen","year":"2005","journal-title":"Methods"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1177\/1087057108328763","article-title":"Defining balanced conditions for inhibitor screening assays that target bisubstrate enzymes","volume":"14","author":"J Yang","year":"2009","journal-title":"J Biomol Screen"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1177\/1087057111421631","article-title":"An efficient high-throughput screening method for MYST family acetyltransferases, a new class of epigenetic drug targets","volume":"16","author":"H Falk","year":"2011","journal-title":"J Biomol Screen"},{"key":"ref60","first-page":"1","article-title":"Comparative studies of thiol-sensitive fluorogenic probes for HAT assays","volume":"421","author":"T Gao","year":"2012","journal-title":"Anal Bioanal Chem"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"4477","DOI":"10.1021\/jm030191r","article-title":"Identification and prediction of promiscuous aggregating inhibitors among known drugs","volume":"46","author":"J Seidler","year":"2003","journal-title":"J Med Chem"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1021\/jm061317y","article-title":"A high-throughput screen for aggregation-based inhibition in a large compound library","volume":"50","author":"BY Feng","year":"2007","journal-title":"J Med Chem"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"3448","DOI":"10.1021\/jm0340896","article-title":"Effect of detergent on \u201cpromiscuous\u201d inhibitors","volume":"46","author":"AJ Ryan","year":"2003","journal-title":"J Med Chem"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1021\/jm010533y","article-title":"A common mechanism underlying promiscuous inhibitors from virtual and high-throughput screening","volume":"45","author":"SL McGovern","year":"2002","journal-title":"J Med Chem"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/nbt1186","article-title":"Statistical practice in high-throughput screening data analysis","volume":"24","author":"N Malo","year":"2006","journal-title":"Nat Biotech"},{"key":"ref66","doi-asserted-by":"crossref","unstructured":"Gubler H (2006) Methods for statistical analysis, quality assurance and management of primary high-throughput screening data. In: H\u00fcser J, editor. High-Throughput Screening in Drug Discovery. KGaA, Weinheim, FRG: Wiley-VCH Verlag GmbH &amp; Co.","DOI":"10.1002\/9783527609321.ch7"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1177\/1087057105276989","article-title":"Statistical analysis of systematic errors in high-throughput screening","volume":"10","author":"D Kevorkov","year":"2005","journal-title":"J Biomol Screen"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1177\/1087057103258285","article-title":"Improved statistical methods for hit selection in high-throughput screening","volume":"8","author":"C Brideau","year":"2003","journal-title":"J Biomol Screen"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"33716","DOI":"10.1074\/jbc.M402839200","article-title":"Polyisoprenylated benzophenone, garcinol, a natural histone acetyltransferase inhibitor, represses chromatin transcription and alters global gene expression","volume":"279","author":"K Balasubramanyam","year":"2004","journal-title":"J Biol Chem"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1021\/jm800657z","article-title":"Mechanism of p300 specific histone acetyltransferase inhibition by small molecules","volume":"52","author":"M Arif","year":"2009","journal-title":"J Med Chem"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1021\/ja0455547","article-title":"ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens","volume":"127","author":"JR Huth","year":"2005","journal-title":"J Am Chem Soc"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.1002\/rcm.3092","article-title":"Investigating the <italic>in vitro<\/italic> metabolism of fipexide: characterization of reactive metabolites using liquid chromatography\/mass spectrometry","volume":"21","author":"L Sleno","year":"2007","journal-title":"Rapid Commun Mass Spectrom"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1126\/science.1160619","article-title":"Redox-active antibiotics control gene expression and community behavior in divergent bacteria","volume":"321","author":"LEP Dietrich","year":"2008","journal-title":"Science"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.abb.2004.05.010","article-title":"The oxidative mechanism of action of ortho-quinone inhibitors of protein-tyrosine phosphatase alpha is mediated by hydrogen peroxide","volume":"429","author":"MP Bova","year":"2004","journal-title":"Arch Biochem Biophys"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"10095","DOI":"10.1016\/S0040-4020(02)01411-4","article-title":"Chemical studies on antioxidant mechanism of garcinol: analysis of radical reaction products of garcinol with peroxyl radicals and their antitumor activities","volume":"58","author":"S Sang","year":"2002","journal-title":"Tetrahedron"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.4155\/fmc.10.237","article-title":"Observations on screening-based research and some concerning trends in the literature","volume":"2","author":"JB Baell","year":"2010","journal-title":"Future Med Chem"},{"key":"ref77","first-page":"1","article-title":"Understanding histone acetyltransferase Rtt109 structure and function: how many chaperones does it take","volume":"21","author":"S D'Arcy","year":"2011","journal-title":"Curr Opin Struc Biol"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"3614","DOI":"10.1021\/ac203385v","article-title":"Enzymatic immuno-assembly of gold nanoparticles for visualized activity screening of histone-modifying enzymes","volume":"84","author":"Z Zhen","year":"2012","journal-title":"Anal Chem"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0003-2697(03)00346-4","article-title":"Mechanistic considerations in high-throughput screening","volume":"320","author":"RA Copeland","year":"2003","journal-title":"Anal Biochem"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"7274","DOI":"10.1021\/jm061103g","article-title":"Interpreting steep dose-response curves in early inhibitor discovery","volume":"49","author":"BK Shoichet","year":"2006","journal-title":"J Med Chem"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S1046-2023(02)00028-2","article-title":"Assays for the identification and evaluation of histone acetyltransferase inhibitors","volume":"26","author":"GW Aherne","year":"2002","journal-title":"Methods"},{"key":"ref82","doi-asserted-by":"crossref","first-page":"3869","DOI":"10.1093\/nar\/26.16.3869","article-title":"A rapid and sensitive assay for histone acetyl-transferase activity","volume":"26","author":"S Ait-Si-Ali","year":"1998","journal-title":"Nucleic Acids Res"},{"key":"ref83","doi-asserted-by":"crossref","first-page":"18157","DOI":"10.1074\/jbc.274.26.18157","article-title":"Catalytic mechanism and function of invariant glutamic acid 173 from the histone acetyltransferase Gcn5 transcriptional coactivator","volume":"274","author":"KG Tanner","year":"1999","journal-title":"J Biol Chem"},{"key":"ref84","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1006\/abio.2001.5340","article-title":"High-throughput screening for identification of small molecule inhibitors of histone acetyltransferases using scintillating microplates (FlashPlate)","volume":"298","author":"F Turlais","year":"2001","journal-title":"Anal Biochem"},{"key":"ref85","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ab.2010.03.025","article-title":"An electrophoretic mobility shift assay for the identification and kinetic analysis of acetyl transferase inhibitors","volume":"402","author":"C Fanslau","year":"2010","journal-title":"Anal Biochem"},{"key":"ref86","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1006\/abio.2000.4546","article-title":"A continuous, nonradioactive assay for histone acetyltransferases","volume":"280","author":"Y Kim","year":"2000","journal-title":"Anal Biochem"},{"key":"ref87","article-title":"Learning from our mistakes: The \u2018unknown knowns\u2019 in fragment screening","author":"BJ Davis","year":"2013","journal-title":"Bioorg Med Chem Lett:"},{"key":"ref88","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.2174\/092986710793348545","article-title":"False positives in the early stages of drug discovery","volume":"17","author":"R \u0160ink","year":"2010","journal-title":"Curr Med Chem"},{"key":"ref89","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1021\/jm901070c","article-title":"Quantitative analyses of aggregation, autofluorescence, and reactivity artifacts in a screen for inhibitors of a thiol protease","volume":"53","author":"A Jadhav","year":"2010","journal-title":"J Med Chem"},{"key":"ref90","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.cbpa.2010.10.022","article-title":"Redox cycling compounds generate H<sub>2<\/sub>O<sub>2<\/sub> in HTS buffers containing strong reducing reagents-real hits or promiscuous artifacts","volume":"15","author":"PA Johnston","year":"2011","journal-title":"Curr Opin Chem Biol"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1177\/1087057107304113","article-title":"A simple assay for detection of small-molecule redox activity","volume":"12","author":"LA Lor","year":"2007","journal-title":"J Biomol Screen"},{"key":"ref92","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10822-007-9109-z","article-title":"Enhancement of chemical rules for predicting compound reactivity towards protein thiol groups","volume":"21","author":"JT Metz","year":"2007","journal-title":"J Comput Aid Mol Des"},{"key":"ref93","doi-asserted-by":"crossref","first-page":"156","DOI":"10.2174\/187231211796905008","article-title":"Impact of impurities on IC50 values of P450 inhibitors","volume":"5","author":"Z Huang","year":"2011","journal-title":"Drug Metab Lett"},{"key":"ref94","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1021\/np800329j","article-title":"Purity-activity relationships of natural products: the case of anti-TB active ursolic acid","volume":"71","author":"BU Jaki","year":"2008","journal-title":"J Nat Prod"},{"key":"ref95","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1021\/ml3003296","article-title":"Metal impurities cause false positives in high-throughput screening campaigns","volume":"4","author":"JC Hermann","year":"2013","journal-title":"ACS Med Chem Lett"},{"key":"ref96","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1093\/bioinformatics\/btr622","article-title":"PubChem promiscuity: a web resource for gathering compound promiscuity data from PubChem","volume":"28","author":"SA Canny","year":"2009","journal-title":"Bioinformatics"},{"key":"ref97","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/nature06526","article-title":"Reaching for high-hanging fruit in drug discovery at protein-protein interfaces","volume":"450","author":"JA Wells","year":"2007","journal-title":"Nature"},{"key":"ref98","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/nchembio.111","article-title":"Chemical probes for histone-modifying enzymes","volume":"4","author":"PA Cole","year":"2008","journal-title":"Nat Chem Biol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0078877","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T01:18:35Z","timestamp":1588987115000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0078877"}},"subtitle":[],"editor":[{"given":"Tim","family":"Thomas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,18]]},"references-count":98,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0078877","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,18]]}}}