{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:17:34Z","timestamp":1790324254314,"version":"4.1.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T00:00:00Z","timestamp":1384732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0079323","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T03:34:51Z","timestamp":1384832091000},"page":"e79323","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":44,"title":["Extended Safety, Immunogenicity and Efficacy of a Blood-Stage Malaria Vaccine in Malian Children: 24-Month Follow-Up of a Randomized, Double-Blinded Phase 2 Trial"],"prefix":"10.1371","volume":"8","clinical-trial-number":[{"clinical-trial-number":"nct00460525","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"Matthew B.","family":"Laurens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamadou A.","family":"Thera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drissa","family":"Coulibaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amed","family":"Ouattara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdoulaye K.","family":"Kone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ando B.","family":"Guindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Traore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idrissa","family":"Traore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bourema","family":"Kouriba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dapa A.","family":"Diallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Issa","family":"Diarra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Modibo","family":"Daou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amagana","family":"Dolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssouf","family":"Tolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamadou S.","family":"Sissoko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou","family":"Niangaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mady","family":"Sissoko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Takala-Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten E.","family":"Lyke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Blackwelder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Godeaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Vekemans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Claude","family":"Dubois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Ripley","family":"Ballou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Dube","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine","family":"Soisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carter L.","family":"Diggs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"House","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Lanar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheetij","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Gray","family":"Heppner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher V.","family":"Plowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ogobara K.","family":"Doumbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,11,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1086\/646613","article-title":"The potential role of vaccines in the elimination of falciparum malaria and the eventual eradication of malaria","volume":"200","author":"CV Plowe","year":"2009","journal-title":"J Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1016\/j.vaccine.2005.01.142","article-title":"Towards an RTS,S-based, multi-stage, multi-antigen vaccine against falciparum malaria: progress at the Walter Reed Army Institute of Research","volume":"23","author":"DG Heppner Jr","year":"2005","journal-title":"Vaccine"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1128\/IAI.70.6.3101-3110.2002","article-title":"Purification, characterization, and immunogenicity of the refolded ectodomain of the Plasmodium falciparum apical membrane antigen 1 expressed in Escherichia coli","volume":"70","author":"S Dutta","year":"2002","journal-title":"Infect Immun"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1016\/S0140-6736(04)17223-1","article-title":"Efficacy of the RTS,S\/AS02A vaccine against Plasmodium falciparum infection and disease in young African children: randomised controlled trial","volume":"364","author":"PL Alonso","year":"2004","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1086\/600119","article-title":"Long-term safety and efficacy of the RTS,S\/AS02A malaria vaccine in Mozambican children","volume":"200","author":"J Sacarlal","year":"2009","journal-title":"J Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1056\/NEJMoa0807381","article-title":"Efficacy of RTS,S\/AS01E vaccine against malaria in children 5 to 17 months of age","volume":"359","author":"P Bejon","year":"2008","journal-title":"N Engl J Med"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e7611","DOI":"10.1371\/journal.pone.0007611","article-title":"A randomized trial assessing the safety and immunogenicity of AS01 and AS02 adjuvanted RTS,S malaria vaccine candidates in children in Gabon","volume":"4","author":"B Lell","year":"2009","journal-title":"PLoS ONE"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e7302","DOI":"10.1371\/journal.pone.0007302","article-title":"Randomized controlled trial of RTS,S\/AS02D and RTS,S\/AS01E malaria candidate vaccines given according to different schedules in Ghanaian children","volume":"4","author":"S Owusu-Agyei","year":"2009","journal-title":"PLoS ONE"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1016\/S0140-6736(07)61542-6","article-title":"Safety of the RTS,S\/AS02D candidate malaria vaccine in infants living in a highly endemic area of Mozambique: a double blind randomised controlled phase I\/IIb trial","volume":"370","author":"JJ Aponte","year":"2007","journal-title":"Lancet"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1056\/NEJMoa0807773","article-title":"Safety and immunogenicity of RTS,S\/AS02D malaria vaccine in infants","volume":"359","author":"S Abdulla","year":"2008","journal-title":"N Engl J Med"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1086\/656190","article-title":"Evaluation of the safety and immunogenicity of the RTS,S\/AS01E malaria candidate vaccine when integrated in the expanded program of immunization","volume":"202","author":"ST Agnandji","year":"2010","journal-title":"J Infect Dis"},{"key":"ref12","first-page":"273","article-title":"Double blind randomized controlled Phase 1 trial to evaluate the safety and immunogenicity of WRAIR\u2019s MSP1 candidate malaria antigen (FMP1) adjuvanted in GlaxosmithKline Biologicals\u2019 AS02A vs. rabies vaccine in semi-immune adults in Bandiagara, Mali","volume":"71","author":"MA Thera","year":"2004","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.vaccine.2009.10.022","article-title":"Evaluation of the safety and immunogenicity of Plasmodium falciparum apical membrane antigen 1, merozoite surface protein 1 or RTS,S vaccines with adjuvant system AS02A administered alone or concurrently in rhesus monkeys","volume":"28","author":"S Pichyangkul","year":"2009","journal-title":"Vaccine"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Narum DL, Thomas AW (1994) Differential localization of full-length and processed forms of PF83\/AMA-1 an apical membrane antigen of Plasmodium falciparum merozoites. Mol Biochem Parasitol 67: 59\u201368. 0166\u20136851(94)90096\u20135 [pii].","DOI":"10.1016\/0166-6851(94)90096-5"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Escalante AA, Grebert HM, Chaiyaroj SC, Magris M, Biswas S, et al. (2001) Polymorphism in the gene encoding the apical membrane antigen-1 (AMA-1) of Plasmodium falciparum. X. Asembo Bay Cohort Project. Mol Biochem Parasitol 113: 279\u2013287. S0166\u20136851(01)00229\u20138 [pii].","DOI":"10.1016\/S0166-6851(01)00229-8"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1128\/IAI.71.3.1416-1426.2003","article-title":"Geographical structure of diversity and differences between symptomatic and asymptomatic infections for Plasmodium falciparum vaccine candidate AMA1","volume":"71","author":"A Cortes","year":"2003","journal-title":"Infect Immun"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"7857","DOI":"10.1073\/pnas.0802328105","article-title":"Population structure of the genes encoding the polymorphic Plasmodium falciparum apical membrane antigen 1: implications for vaccine design","volume":"105","author":"J Duan","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Nair M, Hinds MG, Coley AM, Hodder AN, Foley M, et al. (2002) Structure of domain III of the blood-stage malaria vaccine candidate, Plasmodium falciparum apical membrane antigen 1 (AMA1). J Mol Biol 322: 741\u2013753. S0022283602008069 [pii].","DOI":"10.1016\/S0022-2836(02)00806-9"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"12736","DOI":"10.1073\/pnas.0501808102","article-title":"Structure of AMA1 from Plasmodium falciparum reveals a clustering of polymorphisms that surround a conserved hydrophobic pocket","volume":"102","author":"T Bai","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"12488","DOI":"10.1073\/pnas.0701464104","article-title":"Structural basis of antigenic escape of a malaria vaccine candidate","volume":"104","author":"S Dutta","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e8138","DOI":"10.1371\/journal.pone.0008138","article-title":"High antibody titer against apical membrane antigen-1 is required to protect against malaria in the Aotus model","volume":"4","author":"S Dutta","year":"2009","journal-title":"PLoS ONE"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2ra5","DOI":"10.1126\/scitranslmed.3000257","article-title":"Extreme polymorphism in a vaccine antigen and risk of clinical malaria: implications for vaccine development","volume":"1","author":"SL Takala","year":"2009","journal-title":"Sci Transl Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e1465","DOI":"10.1371\/journal.pone.0001465","article-title":"Safety and immunogenicity of an AMA-1 malaria vaccine in Malian adults: results of a phase 1 randomized controlled trial","volume":"3","author":"MA Thera","year":"2008","journal-title":"PLoS ONE"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e9041","DOI":"10.1371\/journal.pone.0009041","article-title":"Safety and immunogenicity of an AMA1 malaria vaccine in Malian children: results of a phase 1 randomized controlled trial","volume":"5","author":"MA Thera","year":"2010","journal-title":"PLoS ONE"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1056\/NEJMoa1008115","article-title":"A field trial to assess a blood-stage malaria vaccine","volume":"365","author":"MA Thera","year":"2011","journal-title":"N Engl J Med"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1093\/infdis\/jis709","article-title":"Molecular basis of allele-specific efficacy of a blood-stage malaria vaccine: vaccine development implications","volume":"207","author":"A Ouattara","year":"2013","journal-title":"J Infect Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1016\/j.vaccine.2004.01.054","article-title":"Incidence of severe Plasmodium falciparum malaria as a primary endpoint for vaccine efficacy trials in Bandiagara, Mali","volume":"22","author":"KE Lyke","year":"2004","journal-title":"Vaccine"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4203","DOI":"10.1016\/j.vaccine.2007.03.012","article-title":"Phase I dose escalation safety and immunogenicity trial of Plasmodium falciparum apical membrane protein (AMA-1) FMP2.1, adjuvanted with AS02A, in malaria-naive adults at the Walter Reed Army Institute of Research","volume":"25","author":"ME Polhemus","year":"2007","journal-title":"Vaccine"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/1475-2875-9-175","article-title":"Lack of allele-specific efficacy of a bivalent AMA1 malaria vaccine","volume":"9","author":"A Ouattara","year":"2010","journal-title":"Malar J"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"K Tamura","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1093\/bioinformatics\/btg359","article-title":"DnaSP, DNA polymorphism analyses by the coalescent and other methods","volume":"19","author":"J Rozas","year":"2003","journal-title":"Bioinformatics"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"604","DOI":"10.4269\/ajtmh.2002.67.604","article-title":"Impact of preseason treatment on incidence of falciparum malaria and parasite density at a site for testing malaria vaccines in Bandiagara, Mali","volume":"67","author":"D Coulibaly","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1186\/1475-2875-9-182","article-title":"WHO policy development processes for a new vaccine: case study of malaria vaccines","volume":"9","author":"J Milstien","year":"2010","journal-title":"Malar J"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0079323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T12:14:04Z","timestamp":1564748044000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0079323"}},"subtitle":[],"editor":[{"given":"Philip","family":"Bejon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,18]]},"references-count":33,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0079323","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,18]]}}}