{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:14:31Z","timestamp":1790342071504,"version":"4.1.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T00:00:00Z","timestamp":1384905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0080028","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:34:56Z","timestamp":1384994096000},"page":"e80028","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus"],"prefix":"10.1371","volume":"8","author":[{"given":"Zheng","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lunbiao","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,11,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1056\/NEJMoa1304459","article-title":"Human infection with a novel avian-origin influenza A (H7N9) virus","volume":"368","author":"R Gao","year":"2013","journal-title":"N Engl J Med"},{"key":"ref2","unstructured":"World Health Organization Number of confirmed human cases of avian influenza A (H7N9) reported to WHO, as of 30 May 2013. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/influenza\/human_animal_interface\/influenza_h7n9\/08_ReportWebH7N9Number.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/influenza\/human_animal_interface\/influenza_h7n9\/08_ReportWebH7N9Number.pdf<\/ext-link>."},{"key":"ref3","unstructured":"Chen Y, Liang W, Yang S, Wu N, Gao H, <etal>et al<\/etal>.. (2013) Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome. Lancet."},{"key":"ref4","unstructured":"Li Q, Zhou L, Zhou M, Chen Z, Li F, <etal>et al<\/etal>.. (2013) Preliminary Report: Epidemiology of the Avian Influenza A (H7N9) Outbreak in China. N Engl J Med."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/JCM.00382-07","article-title":"Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses","volume":"45","author":"P Auewarakul","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jviromet.2007.12.003","article-title":"Development of one-step real-time RT-PCR assay for detection and quantitation of peste des petits ruminants virus","volume":"148","author":"J Bao","year":"2008","journal-title":"J Virol Methods"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1021\/ac061920o","article-title":"Identification of A\/H5N1 influenza viruses using a single gene diagnostic microarray","volume":"79","author":"ED Dawson","year":"2007","journal-title":"Anal Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1196\/annals.1408.008","article-title":"Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry","volume":"1102","author":"R Sampath","year":"2007","journal-title":"Ann N Y Acad Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.jviromet.2013.03.004","article-title":"Development and evaluation of loop-mediated isothermal amplification assay for detection of Crimean Congo hemorrhagic fever virus in Sudan","volume":"190","author":"HA Osman","year":"2013","journal-title":"J Virol Methods"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1743-422X-9-27","article-title":"Development of a SYBR green I based RT-PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti","volume":"9","author":"PK Dash","year":"2012","journal-title":"Virol J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1186\/1746-6148-8-193","article-title":"Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection","volume":"8","author":"A Mauroy","year":"2012","journal-title":"BMC Vet Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mcp.2012.02.001","article-title":"An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus","volume":"26","author":"LJ Perez","year":"2012","journal-title":"Mol Cell Probes"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jviromet.2012.01.018","article-title":"Development of SYBR Green I based real-time PCR assays for quantitative detection of Rice tungro bacilliform virus and Rice tungro spherical virus","volume":"181","author":"S Sharma","year":"2012","journal-title":"J Virol Methods"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.jcv.2012.12.001","article-title":"Development and evaluation of a broad reacting SYBR-green based quantitative real-time PCR for the detection of different hantaviruses","volume":"56","author":"N Mohamed","year":"2013","journal-title":"J Clin Virol"},{"key":"ref15","unstructured":"World Health Organization (2013) Real-time RT-PCR Protocol for the Detection of Avian Influenza A(H7N9) Virus."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1128\/JVI.02005-07","article-title":"The influenza virus resource at the National Center for Biotechnology Information","volume":"82","author":"Y Bao","year":"2008","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1128\/JCM.02015-07","article-title":"Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis","volume":"46","author":"JH Lin","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/S0140-6736(98)01182-9","article-title":"Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus","volume":"351","author":"KY Yuen","year":"1998","journal-title":"Lancet"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.3201\/eid1807.111913","article-title":"Low pathogenic avian influenza A (H7N2) virus infection in immunocompromised adult, New York, USA, 2003","volume":"18","author":"B Ostrowsky","year":"2012","journal-title":"Emerg Infect Dis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.3201\/eid1012.040961","article-title":"Human illness from avian influenza H7N3, British Columbia","volume":"10","author":"SA Tweed","year":"2004","journal-title":"Emerg Infect Dis"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0140-6736(04)15589-X","article-title":"Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands","volume":"363","author":"M Koopmans","year":"2004","journal-title":"Lancet"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Kageyama T, Fujisaki S, Takashita E, Xu H, Yamada S, <etal>et al<\/etal>.. (2013) Genetic analysis of novel avian A(H7N9) influenza viruses isolated from patients in China, February to April 2013. Euro Surveill 18.","DOI":"10.2807\/ese.18.15.20453-en"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1056\/NEJMp1304661","article-title":"Global concerns regarding novel influenza A (H7N9) virus infections","volume":"368","author":"TM Uyeki","year":"2013","journal-title":"N Engl J Med"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Corman V, Eickmann M, Landt O, Bleicker T, Brunink S, <etal>et al<\/etal>.. (2013) Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China. Euro Surveill 18.","DOI":"10.2807\/ese.18.16.20461-en"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1637\/8720-032109-ResNote.1","article-title":"Genetic characterization of variant strains of highly pathogenic avian influenza H5N1 that escaped detection by real-time reverse transcriptase-PCR diagnostic tests","volume":"54","author":"AS Arafa","year":"2010","journal-title":"Avian Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1128\/JCM.42.4.1511-1518.2004","article-title":"SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability","volume":"42","author":"JF Papin","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jcv.2010.04.013","article-title":"Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains","volume":"48","author":"M Weidmann","year":"2010","journal-title":"J Clin Virol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.jcv.2005.05.002","article-title":"Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays","volume":"35","author":"DM Whiley","year":"2006","journal-title":"J Clin Virol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0080028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T20:35:32Z","timestamp":1647462932000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0080028"}},"subtitle":[],"editor":[{"given":"Xue-jie","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,20]]},"references-count":28,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0080028","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,20]]}}}