{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T10:16:41Z","timestamp":1767867401845,"version":"3.49.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0081893","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T22:43:57Z","timestamp":1384469037000},"page":"e81893","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Apoptotic Damage of Pancreatic Ductal Epithelia by Alcohol and Its Rescue by an Antioxidant"],"prefix":"10.1371","volume":"8","author":[{"given":"Jong Bae","family":"Seo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A. Nagana","family":"Gowda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duk-Su","family":"Koh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0092-8674(04)00046-7","article-title":"Cell death: critical control points","volume":"116","author":"NN Danial","year":"2004","journal-title":"Cell"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: a review of programmed cell death","volume":"35","author":"S Elmore","year":"2007","journal-title":"Toxicol Pathol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.tibs.2006.11.001","article-title":"Cell death by necrosis: towards a molecular definition","volume":"32","author":"P Golstein","year":"2007","journal-title":"Trends Biochem Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1126\/science.7878464","article-title":"Apoptosis in the pathogenesis and treatment of disease","volume":"267","author":"CB Thompson","year":"1995","journal-title":"Science"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1007\/BF02849949","article-title":"Alcohol-induced oxidative stress and reduction in oxidation by ascorbate\/L-cys\/ L-met in the testis, ovary, kidney, and lung of rat","volume":"22","author":"R Amanvermez","year":"2005","journal-title":"Adv Ther"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"340A","DOI":"10.1111\/j.1530-0277.1996.tb01804.x","article-title":"Ethanol-induced apoptosis and oxidative stress in hepatocytes","volume":"20","author":"H Higuchi","year":"1996","journal-title":"Alcohol Clin Exp Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1023\/A:1018878400935","article-title":"Gastroprotection by 4-methylpyrazole against ethanol in humans","volume":"43","author":"G Iaquinto","year":"1998","journal-title":"Dig Dis Sci"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.1742-4658.2009.07505.x","article-title":"Prohibitin is expressed in pancreatic beta-cells and protects against oxidative and proapoptotic effects of ethanol","volume":"277","author":"JH Lee","year":"2010","journal-title":"FEBS J"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1006\/nbdi.2001.0475","article-title":"Ethanol-induced caspase-3 activation in the in vivo developing mouse brain","volume":"9","author":"JW Olney","year":"2002","journal-title":"Neurobiol Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1002\/jnr.10767","article-title":"Ethanol-induced oxidative stress precedes mitochondrially mediated apoptotic death of cultured fetal cortical neurons","volume":"74","author":"V Ramachandran","year":"2003","journal-title":"J Neurosci Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1038\/sj.cdd.4401277","article-title":"Ethanol-induced neuronal apoptosis in vivo requires BAX in the developing mouse brain","volume":"10","author":"C Young","year":"2003","journal-title":"Cell Death Differ"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e19111","DOI":"10.1371\/journal.pone.0019111","article-title":"Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts","volume":"6","author":"CC Chen","year":"2011","journal-title":"PLOS ONE"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/toxsci\/65.2.166","article-title":"Mechanisms of hepatotoxicity","volume":"65","author":"H Jaeschke","year":"2002","journal-title":"Toxicol Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.brainres.2009.06.029","article-title":"Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells","volume":"1285","author":"DS Sheth","year":"2009","journal-title":"Brain Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jhep.2007.02.008","article-title":"Quercetin protects human hepatocytes from ethanol-derived oxidative stress by inducing heme oxygenase-1 via the MAPK\/Nrf2 pathways","volume":"47","author":"P Yao","year":"2007","journal-title":"J Hepatol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.ejphar.2009.11.028","article-title":"Rutin inhibits hydrogen peroxide-induced apoptosis through regulating reactive oxygen species mediated mitochondrial dysfunction pathway in human umbilical vein endothelial cells","volume":"628","author":"G Gong","year":"2010","journal-title":"Eur J Pharmacol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.brainres.2005.03.054","article-title":"Neuroprotective effect of N-acetyl cysteine on hypoxia-induced oxidative stress in primary hippocampal culture","volume":"1046","author":"K Jayalakshmi","year":"2005","journal-title":"Brain Res"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.neulet.2006.09.089","article-title":"Epigallocatechin gallate inhibits nitric oxide-induced apoptosis in rat PC12 cells","volume":"411","author":"JY Jung","year":"2007","journal-title":"Neurosci Lett"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1128\/MCB.15.5.2359","article-title":"Tumor necrosis factor alpha-induced apoptosis in human neuronal cells: protection by the antioxidant N-acetylcysteine and the genes bcl-2 and crmA","volume":"15","author":"AK Talley","year":"1995","journal-title":"Mol Cell Biol"},{"key":"ref20","first-page":"245","article-title":"Overview: how is alcohol metabolized by the body?","volume":"29","author":"S Zakhari","year":"2006","journal-title":"Alcohol Res Health"},{"key":"ref21","first-page":"1570","article-title":"A review: acute and chronic effects of ethanol and alcoholic beverages on the pancreatic exocrine secretion in vivo and in vitro","volume":"22","author":"E Niebergall-Roth","year":"1998","journal-title":"Alcohol Clin Exp Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1097\/00006676-200311000-00007","article-title":"Role of alcohol metabolism in alcoholic pancreatitis","volume":"27","author":"JS Wilson","year":"2003","journal-title":"Pancreas"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"10738","DOI":"10.1073\/pnas.0403431101","article-title":"Ethanol toxicity in pancreatic acinar cells: mediation by nonoxidative fatty acid metabolites","volume":"101","author":"DN Criddle","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0016-5085(97)70106-9","article-title":"Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol","volume":"113","author":"J Werner","year":"1997","journal-title":"Gastroenterology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/alcalc\/agm044","article-title":"Ethanol-induced cytotoxicity in rat pancreatic acinar AR42J cells: role of fatty acid ethyl esters","volume":"43","author":"H Wu","year":"2008","journal-title":"Alcohol Alcohol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1053\/j.gastro.2012.10.037","article-title":"Effects of oxidative alcohol metabolism on the mitochondrial permeability transition pore and necrosis in a mouse model of alcoholic pancreatitis","volume":"144","author":"N Shalbueva","year":"2013","journal-title":"Gastroenterology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1097\/MOG.0b013e328349e2f8","article-title":"Genetics of pancreatitis","volume":"27","author":"J LaRusch","year":"2011","journal-title":"Curr Opin Gastroenterol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1097\/MOG.0b013e32832e06ce","article-title":"Molecular and cellular regulation of pancreatic duct cell function","volume":"25","author":"MC Steward","year":"2009","journal-title":"Curr Opin Gastroenterol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.3748\/wjg.14.1027","article-title":"Pancreatic ductal system obstruction and acute recurrent pancreatitis","volume":"14","author":"M Delhaye","year":"2008","journal-title":"World J Gastroenterol"},{"key":"ref30","first-page":"977","article-title":"Dog pancreatic duct epithelial cells: long-term culture and characterization","volume":"148","author":"D Oda","year":"1996","journal-title":"Am J Pathol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1085\/jgp.116.4.507","article-title":"Regulation of exocytosis by protein kinases and Ca(2+) in pancreatic duct epithelial cells","volume":"116","author":"DS Koh","year":"2000","journal-title":"J Gen Physiol"},{"key":"ref32","first-page":"e50514","article-title":"Isolation and culture of mouse primary pancreatic acinar cells","author":"J Gout","year":"2013","journal-title":"J Vis Exp"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/cyto.990170108","article-title":"A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells","volume":"17","author":"F Belloc","year":"1994","journal-title":"Cytometry"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1038\/nprot.2009.143","article-title":"Expression, purification and use of recombinant annexin V for the detection of apoptotic cells","volume":"4","author":"SE Logue","year":"2009","journal-title":"Nat Protoc"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/9780470511848.ch13","article-title":"Acetaldehyde adducts in circulation","volume":"285","author":"O Niemel\u00e4","year":"2007","journal-title":"Novartis Found Symp"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1002\/bjs.1800760832","article-title":"Pancreatic duct pressure, duct permeability and acute pancreatitis","volume":"76","author":"MH Harvey","year":"1989","journal-title":"Br J Surg"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1023\/B:HIJO.0000039838.56131.02","article-title":"Ethanol increases the paracellular permeability of monolayers of CAPAN-1 pancreatic duct cells","volume":"35","author":"BM Rotoli","year":"2004","journal-title":"J Mol Histol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1097\/00006676-200311000-00006","article-title":"Alcohol and zymogen activation in the pancreatic acinar cell","volume":"27","author":"FS Gorelick","year":"2003","journal-title":"Pancreas"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1053\/gast.2002.30302","article-title":"Ethanol metabolism and transcription factor activation in pancreatic acinar cells in rats","volume":"122","author":"AS Gukovskaya","year":"2002","journal-title":"Gastroenterology"},{"key":"ref40","first-page":"325","article-title":"Rat pancreatic B-cells after chronic alcohol feeding. A morphometric and fine structural study","volume":"10","author":"V Koko","year":"1995","journal-title":"Histol Histopathol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.alcohol.2011.04.001","article-title":"Ethanol causes endoplasmic reticulum stress and impairment of insulin secretion in pancreatic beta-cells","volume":"46","author":"KH Nguyen","year":"2012","journal-title":"Alcohol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s10565-008-9067-9","article-title":"Effects of ethanol on pancreatic beta-cell death: interaction with glucose and fatty acids","volume":"25","author":"K Dembele","year":"2009","journal-title":"Cell Biol Toxicol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"C1431","DOI":"10.1152\/ajpcell.00373.2004","article-title":"Dual effects of n-alcohols on fluid secretion from guinea pig pancreatic ducts","volume":"288","author":"H Hamada","year":"2005","journal-title":"Am J Physiol Cell Physiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1113\/jphysiol.2003.048827","article-title":"Ethanol induces fluid hypersecretion from guinea-pig pancreatic duct cells","volume":"551","author":"A Yamamoto","year":"2003","journal-title":"J Physiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.forsciint.2009.03.010","article-title":"Age and gender differences in blood-alcohol concentration in apprehended drivers in relation to the amounts of alcohol consumed","volume":"188","author":"AW Jones","year":"2009","journal-title":"Forensic Sci Int"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.bbamcr.2003.10.017","article-title":"Toxicity of ethanol and acetaldehyde in hepatocytes treated with ursodeoxycholic or tauroursodeoxycholic acid","volume":"1644","author":"K Henzel","year":"2004","journal-title":"Biochim Biophys Acta"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1001\/jama.1976.03270150034027","article-title":"Unusually high levels of blood alcohol?","volume":"236","author":"B Lindblad","year":"1976","journal-title":"JAMA"},{"key":"ref48","first-page":"293","article-title":"Blood methanol concentrations during experimentally induced ethanol intoxication in alcoholics","volume":"179","author":"E Majchrowicz","year":"1971","journal-title":"J Pharmacol Exp Ther"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/jnc.12076","article-title":"Intracellular calcium plays a critical role in the alcohol-mediated death of cerebellar granule neurons","volume":"124","author":"DE Kouzoukas","year":"2013","journal-title":"J Neurochem"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"G1614","DOI":"10.1152\/ajpgi.00273.2006","article-title":"Ethanol induced NF-{kappa}B activation protects against cell injury in cultured rat gastric mucosal epithelium","volume":"292","author":"H Mustonen","year":"2007","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1016\/j.bcp.2012.07.019","article-title":"Globular adiponectin inhibits ethanol-induced apoptosis in HepG2 cells through heme oxygenase-1 induction","volume":"84","year":"2012","journal-title":"Biochem Pharmacol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/pcan.2012.37","article-title":"Ethanol promotes cytotoxic effects of tumor necrosis factor-related apoptosis-inducing ligand through induction of reactive oxygen species in prostate cancer cells","volume":"16","author":"MK Plante","year":"2013","journal-title":"Prostate Cancer Prostatic Dis"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/j.1530-0277.2010.01358.x","article-title":"Ethanol effect on cell proliferation in the human hepatoma HepaRG cell line: relationship with iron metabolism","volume":"35","author":"TH Tuoi Do","year":"2011","journal-title":"Alcohol Clin Exp Res"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1475-2867-13-63","article-title":"Genistein promotes cell death of ethanol-stressed HeLa cells through the continuation of apoptosis or secondary necrosis","volume":"13","author":"X Xie","year":"2013","journal-title":"Cancer Cell Int"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.neuint.2013.05.011","article-title":"The protective effects of resveratrol on Schwann cells with toxicity induced by ethanol in vitro","volume":"63","author":"H Yuan","year":"2013","journal-title":"Neurochem Int"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1097\/00006676-200301000-00005","article-title":"Expression of antioxidant enzymes in diseases of the human pancreas: another link between chronic pancreatitis and pancreatic cancer","volume":"26","author":"JJ Cullen","year":"2003","journal-title":"Pancreas"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.taap.2012.04.010","article-title":"Oxidative stress is involved in Dasatinib-induced apoptosis in rat primary hepatocytes","volume":"261","author":"T Xue","year":"2012","journal-title":"Toxicol Appl Pharmacol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1046\/j.1471-4159.1999.0730220.x","article-title":"Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals: inhibition of alpha-ketoglutarate dehydrogenase","volume":"73","author":"C Chinopoulos","year":"1999","journal-title":"J Neurochem"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"7337","DOI":"10.1074\/jbc.275.10.7337","article-title":"p53 induces apoptosis by caspase activation through mitochondrial cytochrome c release","volume":"275","author":"M Schuler","year":"2000","journal-title":"J Biol Chem"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1111\/j.1530-0277.2009.00927.x","article-title":"Expression pattern, ethanol-metabolizing activities, and cellular localization of alcohol and aldehyde dehydrogenases in human pancreas: implications for pathogenesis of alcohol-induced pancreatic injury","volume":"33","author":"CP Chiang","year":"2009","journal-title":"Alcohol Clin Exp Res"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/clpt.1983.70","article-title":"Disulfiram and erythrocyte aldehyde dehydrogenase inhibition","volume":"33","author":"JF Towell 3rd","year":"1983","journal-title":"Clin Pharmacol Ther"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1073\/pnas.90.4.1247","article-title":"Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase","volume":"90","author":"WM Keung","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1007\/BF01296608","article-title":"Effects of H2-receptor antagonists on gastric alcohol dehydrogenase activity","volume":"36","author":"J Caballer\u00eda","year":"1991","journal-title":"Dig Dis Sci"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1146\/annurev.physiol.67.031103.153247","article-title":"Mechanisms of bicarbonate secretion in the pancreatic duct","volume":"67","author":"MC Steward","year":"2005","journal-title":"Annu Rev Physiol"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/10739680590896009","article-title":"A new hypothesis for microvascular inflammation in shock and multiorgan failure: self-digestion by pancreatic enzymes","volume":"12","author":"GW Schmid-Sch\u00f6nbein","year":"2005","journal-title":"Microcirculation"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0081893","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T09:42:42Z","timestamp":1589017362000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0081893"}},"subtitle":[],"editor":[{"given":"Hiroyasu","family":"Nakano","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,11,14]]},"references-count":65,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0081893","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,14]]}}}