{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:48:22Z","timestamp":1791280102517,"version":"4.1.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0081965","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T16:30:12Z","timestamp":1386088212000},"page":"e81965","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Kinetics of Expansion of Human Limbal Epithelial Progenitor Cells in Primary Culture of Explants Without Feeders"],"prefix":"10.1371","volume":"8","author":[{"given":"Djida","family":"Ghoubay-Benallaoua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otman","family":"Sandali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Goldschmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Borderie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"ref1","first-page":"3","article-title":"Structure and function of the cornea","author":"SD Klyce","year":"1988","journal-title":"Cornea"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1201\/b14144-12","article-title":"Ocular surface epithelial stem cells: implications for ocular surface homeostasis","author":"LPK Ang","year":"2004","journal-title":"Dry Eye and Ocular Surface Disorders"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2217\/17460751.1.1.45","article-title":"Regeneration of squamous epithelia from stem cells of cultured grafts","volume":"1","author":"M De Luca","year":"2006","journal-title":"Regen Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.survophthal.2007.06.013","article-title":"Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results","volume":"52","author":"AJ Shortt","year":"2007","journal-title":"Surv Ophthalmol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1517\/14712598.7.1.1","article-title":"Limbal epithelial stem cell therapy","volume":"7","author":"JT Daniels","year":"2007","journal-title":"Expert Opin Biol Ther"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.semcdb.2007.09.011","article-title":"Towards therapeutic application of ocular stem cells","volume":"18","author":"G Pellegrini","year":"2007","journal-title":"Semin Cell Dev Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1056\/NEJM200007133430202","article-title":"Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells","volume":"343","author":"RJ Tsai","year":"2000","journal-title":"N Engl J Med"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1016\/S0161-6420(01)00694-7","article-title":"Cultivated corneal epithelial stem cell transplantation in ocular surface disorders","volume":"108","author":"N Koizumi","year":"2001","journal-title":"Ophthalmology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/S0161-6420(02)01089-8","article-title":"Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders","volume":"109","author":"J Shimazaki","year":"2002","journal-title":"Ophthalmology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1016\/S0161-6420(02)01105-3","article-title":"Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deficiency","volume":"109","author":"M Grueterich","year":"2002","journal-title":"Ophthalmology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1023\/B:BIRE.0000007690.43273.73","article-title":"Successful reconstruction of damaged ocular outer surface in humans using limbal and conjunctival stem cell culture methods","volume":"23","author":"VS Sangwan","year":"2003","journal-title":"Biosci Rep"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1111\/j.1395-3907.2004.00285.x","article-title":"Successful primary culture and autologous transplantation of corneal limbal epithelial cells from minimal biopsy for unilateral severe ocular surface disease","volume":"82","author":"T Nakamura","year":"2004","journal-title":"Acta Ophthalmol Scand"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e46734","DOI":"10.1371\/journal.pone.0046734","article-title":"Nucleic acid and non-nucleic acid-based reprogramming of adult limbal progenitors to pluripotency","volume":"7","author":"S Parameswaran","year":"2012","journal-title":"PLOS ONE"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1089\/ten.tec.2010.0609","article-title":"A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells","volume":"17","author":"SY Chen","year":"2011","journal-title":"Tissue Eng Part C Methods"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1083\/jcb.103.1.49","article-title":"Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells","volume":"103","author":"A Schermer","year":"1986","journal-title":"J Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/eye.1994.41","article-title":"Perpetuation of stem cells in the eye","volume":"8","author":"JD Zieske","year":"1994","journal-title":"Eye"},{"key":"ref17","first-page":"1336","article-title":"Epithelial regeneration after limbus-to-limbus debridement. Expression of alphaenolase in stem and transient amplifying cells","volume":"36","author":"EH Chung","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1002\/dvdy.20050","article-title":"Regional distribution of alpha9beta1 integrin within the limbus of the mouse ocular surface","volume":"230","author":"A Pajoohesh-Ganji","year":"2004","journal-title":"Dev Dyn"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1073\/pnas.061032098","article-title":"P63 identifies keratinocyte stem cells","volume":"98","author":"G Pellegrini","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1242\/jcs.02279","article-title":"Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells","volume":"118","author":"MT Budak","year":"2005","journal-title":"J Cell Sci"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1634\/stemcells.2005-0064","article-title":"Limbal epithelial side-population cells have stem cell-like properties, including quiescent state","volume":"24","author":"T Umemoto","year":"2006","journal-title":"Stem Cells"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1002\/stem.1393","article-title":"PEDF Promotes Self-Renewal of Limbal Stem Cell and Accelerates Corneal Epithelial Wound Healing","volume":"31","author":"TC Ho","year":"2013","journal-title":"Stem Cells"},{"key":"ref23","first-page":"159","article-title":"Human corneal stem cells display functional neuronal properties","volume":"30","author":"GM Seigel","year":"2003","journal-title":"Mol Vis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1006\/dbio.2002.0793","article-title":"Adult corneal limbal epithelium: a model for studying neural potential of non-neural stem cells\/progenitors","volume":"250","author":"X Zhao","year":"2002","journal-title":"Dev Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1634\/stemcells.22-3-355","article-title":"Characterization of putative stem cell phenotype in human limbal epithelia","volume":"22","author":"Z Chen","year":"2004","journal-title":"Stem Cells"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.febslet.2004.03.064","article-title":"Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG2","volume":"565","author":"K Watanabe","year":"2004","journal-title":"FEBS Lett"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1083\/jcb.200703003","article-title":"C\/EBPdelta regulates cell cycle and self-renewal of human limbal stem cells","volume":"177","author":"V Barbaro","year":"2007","journal-title":"J Cell Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/path.2441","article-title":"Epithelial stem cells in corneal regeneration and epidermal gene therapy","volume":"217","author":"G Pellegrini","year":"2009","journal-title":"J Pathol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1056\/NEJMoa0905955","article-title":"Limbal stem-cell therapy and long-term corneal regeneration","volume":"363","author":"P Rama","year":"2010","journal-title":"N Engl J Med"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1634\/stemcells.2006-0677","article-title":"Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells","volume":"25","author":"YT Chen","year":"2007","journal-title":"Stem Cells"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1136\/bjo.2006.103218","article-title":"A novel method for preserving cultured limbal epithelial cells","volume":"91","author":"TP Utheim","year":"2007","journal-title":"Br J Ophthalmol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1167\/iovs.09-4109","article-title":"Preservation of the limbal stem cell phenotype by appropriate culture techniques","volume":"51","author":"EA Meyer-Blazejewska","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref33","first-page":"341","article-title":"Human epithelial cell cultures from superficial limbal explants","volume":"17","author":"D Ghoubay-Benallaoua","year":"2011","journal-title":"Mol Vis"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"644","DOI":"10.3109\/02713683.2012.669510","article-title":"Effects of isoproterenol and cholera toxin on human limbal epithelial cell cultures","volume":"37","author":"D Ghoubay-Benallaoua","year":"2012","journal-title":"Curr Eye Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1167\/iovs.03-1361","article-title":"Development of a conjunctival epithelial equivalent with improved proliferative properties using a multistep serum-free culture system","volume":"45","author":"LP Ang","year":"2004","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1167\/iovs.05-1188","article-title":"The use of autologous serum in the development of corneal and oral epithelial equivalents in patients with Stevens-Johnson syndrome","volume":"47","author":"T Nakamura","year":"2006","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1167\/iovs.06-1448","article-title":"Human corneal epithelial equivalents for ocular surface reconstruction in a complete <underline>serum-free<\/underline> <underline>culture<\/underline> <underline>system<\/underline> <underline>without<\/underline> <underline>unknown<\/underline> <underline>factors<\/underline>","volume":"49","author":"S Yokoo","year":"2008","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1136\/bjo.82.4.382","article-title":"Donor organ cultured corneal tissue selection before penetrating keratoplasty","volume":"82","author":"VM Borderie","year":"1998","journal-title":"Br J Ophthalmol"},{"key":"ref39","first-page":"9523","article-title":"Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration","volume":"102","author":"E Di Iorio","year":"2005"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1083\/jcb.145.4.769","article-title":"Location and clonal analysis of stem cells and their differentiated progeny in human ocular surface","volume":"145","author":"G Pellegrini","year":"1999","journal-title":"J Cell Biol"},{"key":"ref41","first-page":"549","article-title":"Effects of preservation time on proliferative potential of human limbal stem\/progenitor cells","volume":"5","author":"T Liu","year":"2012","journal-title":"Int J Ophthalmol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1167\/iovs.06-0514","article-title":"The fate of limbal epithelial progenitor cells during explant culture on intact amniotic membrane","volume":"48","author":"W Li","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref43","article-title":"Epidermal differentiation and loss of clonal growth potential of human limbal basal epithelial progenitor cells during intrastromal invasion 8;52","author":"EK Tan","year":"2011"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/19539","article-title":"p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development","volume":"398","author":"A Yang","year":"1999","journal-title":"Nature"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.cell.2007.02.045","article-title":"p63 is essential for the proliferative potential of stem cells in stratified epithelia","volume":"129","author":"M Senoo","year":"2007","journal-title":"Cell"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1126\/science.7914031","article-title":"Homology of the eyeless gene of <italic>Drosophila<\/italic> to the Small eye gene in mice and Aniridia in humans","volume":"265","author":"R Quiring","year":"1994","journal-title":"Science"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0081965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T03:23:41Z","timestamp":1540005821000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0081965"}},"subtitle":[],"editor":[{"given":"Olaf","family":"Strau\u00df","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,12,3]]},"references-count":46,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,12,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0081965","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,3]]}}}