{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:09:43Z","timestamp":1767863383404,"version":"3.49.0"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T00:00:00Z","timestamp":1388102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0082765","type":"journal-article","created":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T21:12:21Z","timestamp":1388178741000},"page":"e82765","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Intranasal Immunisation with Recombinant Toxoplasma gondii Actin Partly Protects Mice against Toxoplasmosis"],"prefix":"10.1371","volume":"8","author":[{"given":"Li-Tian","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Xia","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Long","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Li","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Rong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2013,12,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.ijpara.2003.11.023","article-title":"<italic>Toxoplasma gondii<\/italic> and mucosal immunity","volume":"34","author":"L Kasper","year":"2004","journal-title":"Int J Parasitol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1126\/science.1094717","article-title":"Intracellular parasite invasion strategies","volume":"304","author":"LD Sibley","year":"2004","journal-title":"Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"S65","DOI":"10.1017\/S0031182000017339","article-title":"Parasitic diseases and immunodeficiencies","volume":"122","author":"P Ambroise-Thomas","year":"2001","journal-title":"Parasitology"},{"key":"ref4","first-page":"704","article-title":"Cellular immune responses to recombinant antigens in pregnant women chronically infected with <italic>Toxoplasma gondii<\/italic>","volume":"9","author":"AF Fatoohi","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/B978-0-444-52910-7.00028-3","article-title":"Congenital toxoplasmosis","volume":"112","author":"F Kieffer","year":"2013","journal-title":"Handb Clin Neurol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2007.07.003","article-title":"<italic>Toxoplasma gondii<\/italic> and ovine toxoplasmosis: new aspects of an old story","volume":"149","author":"D Buxton","year":"2007","journal-title":"Vet Parasitol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1089\/fpd.2010.0795","article-title":"<italic>Toxoplasma<\/italic> in animals, food, and humans: an old parasite of new concern","volume":"8","author":"BT Cenci-Goga","year":"2011","journal-title":"Foodborne Pathog Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1586\/14760584.8.2.215","article-title":"Vaccination concepts against <italic>Toxoplasma gondii<\/italic>","volume":"8","author":"JL Garcia","year":"2009","journal-title":"Expert Rev Vaccines"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1017\/S003118200000144X","article-title":"A commercial vaccine for ovine toxoplasmosis","volume":"110","author":"D Buxton","year":"1995","journal-title":"Parasitology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1590\/S0074-02762009000200018","article-title":"Veterinary vaccines against <italic>Toxoplasma gondii<\/italic>","volume":"104","author":"EA Innes","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/rmv.307","article-title":"Mucosal immune network in the gut for the control of infectious diseases","volume":"11","author":"H Iijima","year":"2001","journal-title":"Rev Med Virol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1590\/S0074-02762009000200019","article-title":"Vaccines against <italic>Toxoplasma gondii<\/italic>: challenges and opportunities","volume":"104","author":"E Jongert","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/nbt.1742","article-title":"Efficient mucosal vaccination mediated by the neonatal Fc receptor","volume":"29","author":"L Ye","year":"2011","journal-title":"Nat Biotechnol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1365-3083.2009.02319.x","article-title":"Mucosal immunity: induction, dissemination, and effector functions","volume":"70","author":"P Brandtzaeg","year":"2009","journal-title":"Scand J Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1128\/IAI.68.2.969-972.2000","article-title":"Intranasal immunization with <italic>Toxoplasma gondii<\/italic> SAG1 induces protective cells into both NALT and GALT compartments","volume":"68","author":"F Velge-Roussel","year":"2000","journal-title":"Infect Immun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0264-410X(97)00168-0","article-title":"Induction of a mucosal and systemic immune response by intranasal immunization using recombinant cholera toxin B subunit as adjuvant","volume":"16","author":"HY Wu","year":"1998","journal-title":"Vaccine"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"771","DOI":"10.3109\/1061186X.2010.523790","article-title":"Nasal vaccine innovation","volume":"18","author":"I Jabbal-Gill","year":"2010","journal-title":"J Drug Target"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1128\/IAI.64.6.2158-2166.1996","article-title":"Intranasal immunization with SAG1 protein of <italic>Toxoplasma gondii<\/italic> in association with cholera toxin dramatically reduces development of cerebral cysts after oral infection","volume":"64","author":"N Debard","year":"1996","journal-title":"Infect Immun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1016\/j.micinf.2006.07.018","article-title":"Intranasal administration of adjuvant-combined recombinant influenza virus HA vaccine protects mice from the lethal H5N1 virus infection","volume":"8","author":"Y Asahi-Ozaki","year":"2006","journal-title":"Microbes Infect"},{"key":"ref20","first-page":"290","article-title":"Intranasal immunization with mucosal complex vaccine protects mice against <italic>Toxoplasma gondii<\/italic>","volume":"25","author":"GR Yin","year":"2007","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0169-4758(88)90113-5","article-title":"Cell motility of sporozoan protozoa","volume":"4","author":"CA King","year":"1988","journal-title":"Parasitol Today"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1111\/j.1550-7408.1994.tb06086.x","article-title":"Divalent cation and ATP dependent motility of <italic>Toxoplasma gondii<\/italic> tachyzoites after mild treatment with trypsin","volume":"41","author":"R Mondragon","year":"1994","journal-title":"J Eukaryot Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1091\/mbc.10.11.3539","article-title":"Time-lapse video microscopy of gliding motility in <italic>Toxoplasma gondii<\/italic> reveals a novel, biphasic mechanism of cell locomotion","volume":"10","author":"S Hakansson","year":"1999","journal-title":"Mol Biol Cell"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.ijpara.2005.03.016","article-title":"Identification and purification of actin from the subpellicular network of <italic>Toxoplasma gondii<\/italic> tachyzoites","volume":"35","author":"SA Patr\u00f3n","year":"2005","journal-title":"Int J Parasitol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1128\/EC.00064-08","article-title":"A novel actin-related protein is associated with daughter cell formation in <italic>Toxoplasma gondii<\/italic>","volume":"7","author":"JL Gordon","year":"2008","journal-title":"Eukaryotic Cell"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/(SICI)1097-0169(1997)37:3<253::AID-CM7>3.0.CO;2-7","article-title":"Actin in the parasite <italic>Toxoplasma gondii<\/italic> is encoded by a single copy gene, ACT1 and exists primarily in a globular form","volume":"37","author":"JM Dobrowolski","year":"1997","journal-title":"Cell Motil Cytoskeleton"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e1001132","DOI":"10.1371\/journal.ppat.1001132","article-title":"Concerted action of two formins in gliding motility and host cell invasion by <italic>Toxoplasma gondii<\/italic>","volume":"6","author":"W Daher","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref28","first-page":"352","article-title":"Cloning, expression and antigenicity analysis of actin gene of <italic>Toxoplasma gondii<\/italic>","volume":"31","author":"RH Li","year":"2013","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2741","DOI":"10.1016\/j.vaccine.2009.03.013","article-title":"Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against <italic>Toxoplasma gondii<\/italic> challenge","volume":"27","author":"L Shang","year":"2009","journal-title":"Vaccine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.1016\/j.vaccine.2011.01.089","article-title":"Induction of protective Th1 immune responses in mice by vaccination with recombinant <italic>Toxoplasma gondii<\/italic> nucleoside triphosphate hydrolase-II","volume":"29","author":"F Tan","year":"2011","journal-title":"Vaccine"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e70884","DOI":"10.1371\/journal.pone.0070884","article-title":"<italic>Toxoplasma gondii<\/italic> protein disulfide isomerase (TgPDI) is a novel vaccine candidate against toxoplasmosis","volume":"8","author":"HL Wang","year":"2013","journal-title":"PLoS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.vetpar.2013.06.013","article-title":"Evaluation of a real-time PCR assay based on the single-copy SAG1 gene for the detection of <italic>Toxoplasma gondii<\/italic>","volume":"197","author":"H Yu","year":"2013","journal-title":"Vet Parasitol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s13346-012-0085-z","article-title":"Strategies for intranasal delivery of vaccines","volume":"3","author":"M Zaman","year":"2013","journal-title":"Drug Deliv Transl Res"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0161-5890(93)90064-I","article-title":"Vaccination of mice with the protective F3G3 antigen of <italic>Toxoplasma gondii<\/italic> activates CD41 but not CD81 T cells and induces <italic>Toxoplasma<\/italic> specific IgG antibody","volume":"30","author":"V Brinkmann","year":"1993","journal-title":"Mol Immunol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1128\/IAI.61.6.2639-2643.1993","article-title":"Immune responses and resistance to <italic>Toxoplasma gondii<\/italic> in mice immunized with antigens of the parasite incorporated into immunostimulating complexes","volume":"61","author":"A Lund\u00e9n","year":"1993","journal-title":"Infect Immun"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1128\/IAI.64.4.1272-1283.1996","article-title":"Salivary, nasal, genital, and systemic antibody responses in monkeys immunized intranasally with a bacterial protein antigen and the cholera toxin B subunit","volume":"64","author":"MW Russell","year":"1996","journal-title":"Infect Immun"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.exppara.2007.10.002","article-title":"<italic>Toxoplasma gondii<\/italic>: evaluation of an intranasal vaccine using recombinant proteins against brain cyst formation in BALB\/c mice","volume":"118","author":"M Igarashi","year":"2008","journal-title":"Exp Parasitol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.exppara.2009.04.002","article-title":"<italic>Toxoplasma gondii<\/italic>: protective effect of an intranasal SAG1 and MIC4 DNA vaccine in mice","volume":"122","author":"H Wang","year":"2009","journal-title":"Exp Parasitol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1590\/S1984-29612010000400004","article-title":"<italic>Toxoplasma gondii<\/italic>: humoral and cellular immune response of BALB\/c mice immunized via intranasal route with rTgROP2","volume":"19","author":"M Igarashi","year":"2010","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1016\/j.vaccine.2006.10.060","article-title":"GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine","volume":"25","author":"M D\u00f6\u015fkaya","year":"2007","journal-title":"Vaccine"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.vaccine.2010.11.002","article-title":"Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of <italic>Toxoplasma gondii<\/italic> in murine models of experimental toxoplasmosis","volume":"29","author":"B Dziadek","year":"2011","journal-title":"Vaccine"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.exppara.2009.06.002","article-title":"<italic>Toxoplasma gondii<\/italic>: the immunogenic and protective efficacy of recombinant ROP2 and ROP4 rhoptry proteins in murine experimental toxoplasmosis","volume":"123","author":"B Dziadek","year":"2009","journal-title":"Exp Parasitol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1016\/j.vaccine.2007.02.057","article-title":"Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A (MPL) adjuvant against <italic>Toxoplasma<\/italic> chronic infection in mice","volume":"25","author":"M Golkar","year":"2007","journal-title":"Vaccine"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.exppara.2011.04.004","article-title":"Combination of CpG-oligodeoxynucleotides with recombinant ROP2 or GRA4 proteins induces protective immunity against <italic>Toxoplasma gondii<\/italic> infection","volume":"128","author":"VR S\u00e1nchez","year":"2011","journal-title":"Exp Parasitol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.exppara.2012.01.001","article-title":"<italic>Toxoplasma gondii<\/italic>: protective immunity against toxoplasmosis with recombinant actin depolymerizing factor protein in BALB\/c mice","volume":"130","author":"X Huang","year":"2012","journal-title":"Exp Parasitol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"755","DOI":"10.2217\/bmm.10.81","article-title":"Cytokine patterns after therapy with Avonex\u00ae, Rebif\u00ae, Betaferon\u00ae and CinnoVex\u00ae in relapsing-remitting multiple sclerosis in Iranian patients","volume":"4","author":"MK Arababadi","year":"2010","journal-title":"Biomark Med"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1016\/j.vaccine.2012.01.073","article-title":"<italic>Toxoplasma gondii<\/italic> immune mapped protein-1 (TgIMP1) is a novel vaccine candidate against toxoplasmosis","volume":"30","author":"H Zhou","year":"2012","journal-title":"Vaccine"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1016\/j.vaccine.2012.01.004","article-title":"Protective immune response against <italic>Toxoplasma gondii<\/italic> elicited by a recombinant DNA vaccine with a novel genetic adjuvant","volume":"30","author":"H Zhou","year":"2012","journal-title":"Vaccine"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"6570","DOI":"10.1016\/j.vaccine.2009.08.043","article-title":"<italic>Toxoplasma gondii<\/italic> microneme protein 6 (MIC6) is a potential vaccine candidate against toxoplasmosis in mice","volume":"27","author":"GH Peng","year":"2009","journal-title":"Vaccine"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1128\/CVI.00312-10","article-title":"Protective immunity induced by <italic>Toxoplasma gondii<\/italic> rhoptry protein 16 against toxoplasmosis in Mice","volume":"18","author":"ZG Yuan","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"4578","DOI":"10.1016\/j.vaccine.2013.07.058","article-title":"The virulence-related rhoptry protein 5 (ROP5) of <italic>Toxoplasma gondii<\/italic> is a novel vaccine candidate against toxoplasmosis in mice","volume":"31","author":"B Zheng","year":"2013","journal-title":"Vaccine"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"5631","DOI":"10.1016\/j.vaccine.2012.06.081","article-title":"Enhancement of protective immune responses induced by <italic>Toxoplasma gondii<\/italic> dense granule antigen 7 (GRA7) against toxoplasmosis in mice using a prime-boost vaccination strategy","volume":"30","author":"J Min","year":"2012","journal-title":"Vaccine"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/1756-3305-6-175","article-title":"Identification and characterization of <italic>Toxoplasma gondii<\/italic> aspartic protease 1 as a novel vaccine candidate against toxoplasmosis","volume":"6","author":"G Zhao","year":"2013","journal-title":"Parasit Vectors"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"112","DOI":"10.5001\/omj.2013.29","article-title":"Interleukin-10 serum levels after vaccination with <italic>in vivo<\/italic> prepared <italic>Toxoplasma gondii<\/italic> excreted\/secreted antigens","volume":"28","author":"SH Abdollahi","year":"2013","journal-title":"Oman Med J"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1128\/IAI.59.3.977-982.1991","article-title":"New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against <italic>Vibrio cholerae<\/italic> infection","volume":"59","author":"L Winner 3rd","year":"1991","journal-title":"Infect Immun"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1128\/IAI.60.5.1786-1792.1992","article-title":"Monoclonal secretory immunoglobulin A protects mice against oral challenge with the invasive pathogen <italic>Salmonella typhimurium<\/italic>","volume":"60","author":"P Michetti","year":"1992","journal-title":"Infect Immun"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1128\/JVI.78.2.947-957.2004","article-title":"Secretory immunoglobulin A antibodies against the sigma1 outer capsid protein of reovirus type 1 Lang prevent infection of mouse Peyer&apos;s patches","volume":"78","author":"AB Hutchings","year":"2004","journal-title":"J Virol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"383","DOI":"10.3109\/08820131003622635","article-title":"Secretory IgA: arresting microbial pathogens at epithelial borders","volume":"39","author":"NJ Mantis","year":"2010","journal-title":"Immunol Invest"},{"key":"ref59","first-page":"21","article-title":"Kinetics of IgA secreting cells and IgA in small intestine induced by <italic>Toxoplasma gondii<\/italic> tachyzoite infected mice and the correlation of them","volume":"28","author":"JY Shen","year":"2010","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1128\/IAI.64.6.2158-2166.1996","article-title":"Intranasal immunization with SAG1 protein of <italic>Toxoplasma gondii<\/italic> in association with cholera toxin dramatically reduces development of cerebral cysts after oral infection","volume":"64","author":"N Debard","year":"1996","journal-title":"Infect Immun"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0082765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T23:28:44Z","timestamp":1597274924000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0082765"}},"subtitle":[],"editor":[{"given":"Victoria","family":"Lawson","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,12,27]]},"references-count":60,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,12,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0082765","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,27]]}}}