{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:45:10Z","timestamp":1792449910216,"version":"4.5.0"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T00:00:00Z","timestamp":1390435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0086669","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T23:45:48Z","timestamp":1390520748000},"page":"e86669","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":175,"title":["Evaluation of Epidemiological Cut-Off Values Indicates that Biocide Resistant Subpopulations Are Uncommon in Natural Isolates of Clinically-Relevant Microorganisms"],"prefix":"10.1371","volume":"9","author":[{"given":"Ian","family":"Morrissey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Rinaldo","family":"Oggioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Knight","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Curiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Coque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse","family":"Kalkanci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Luis","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the BIOHYPO Consortium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,1,23]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S137","DOI":"10.1086\/519255","article-title":"Consumer antibacterial soaps: effective or just risky?","volume":"45","author":"AE Aiello","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1365-2672.2009.04395.x","article-title":"Antimicrobial mortar surfaces for the improvement of hygienic conditions","volume":"108","author":"W De Muynck","year":"2010","journal-title":"J Appl Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1067\/mic.2000.102378","article-title":"Triclosan: a review of effectiveness and safety in health care settings","volume":"28","author":"RD Jones","year":"2000","journal-title":"Am J Infect Control"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jhin.2010.05.020","article-title":"Does microbial resistance or adaptation to biocides create a hazard in infection prevention and control?","volume":"76","author":"B Meyer","year":"2010","journal-title":"J Hosp Infect"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S1473-3099(03)00723-0","article-title":"Antibacterial cleaning and hygiene products as an emerging risk factor for antibiotic resistance in the community","volume":"3","author":"AE Aiello","year":"2003","journal-title":"Lancet Infect Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"512","DOI":"10.3201\/eid0707.017705","article-title":"Antibacterial household products: cause for concern","volume":"7","author":"SB Levy","year":"2001","journal-title":"Emerg Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Levy SB (2002) Active efflux, a common mechanism for biocide and antibiotic resistance. Symp Ser Soc Appl Microbiol: 65S\u201371S.","DOI":"10.1046\/j.1365-2672.92.5s1.4.x"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1128\/AAC.49.2.781-782.2005","article-title":"The biocide triclosan selects <italic>Stenotrophomonas maltophilia<\/italic> mutants that overproduce the SmeDEF multidrug efflux pump","volume":"49","author":"P Sanchez","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1089\/mdr.2006.12.83","article-title":"Triclosan and antimicrobial resistance in bacteria: an overview","volume":"12","author":"SP Yazdankhah","year":"2006","journal-title":"Microb Drug Resist"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/jac\/49.1.11","article-title":"Biocide abuse and antimicrobial resistance\u2013a cause for concern?","volume":"49","author":"AP Fraise","year":"2002","journal-title":"J Antimicrob Chemother"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Maillard JY, Bloomfield S, Coelho JR, Collier P, Cookson B, <etal>et al<\/etal>.. (2013) Does Microbicide Use in Consumer Products Promote Antimicrobial Resistance? A Critical Review and Recommendations for a Cohesive Approach to Risk Assessment. Microb Drug Resist.","DOI":"10.1089\/mdr.2013.0039"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1586\/eri.13.16","article-title":"Recent advances in the potential interconnection between antimicrobial resistance to biocides and antibiotics","volume":"11","author":"MR Oggioni","year":"2013","journal-title":"Expert Rev Anti Infect Ther"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1093\/jac\/dkg312","article-title":"European harmonization of MIC breakpoints for antimicrobial susceptibility testing of bacteria","volume":"52","author":"G Kahlmeter","year":"2003","journal-title":"J Antimicrob Chemother"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1046\/j.1365-2672.2003.01825.x","article-title":"Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria","volume":"94","author":"SE Walsh","year":"2003","journal-title":"J Appl Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/S0195-6701(03)00240-8","article-title":"Development of bacterial resistance to several biocides and effects on antibiotic susceptibility","volume":"55","author":"SE Walsh","year":"2003","journal-title":"J Hosp Infect"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/S1473-3099(03)00833-8","article-title":"Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations","volume":"3","author":"AD Russell","year":"2003","journal-title":"Lancet Infect Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/S0966-842X(00)01873-4","article-title":"What is antibiotic resistance and how can we measure it?","volume":"8","author":"HC Davison","year":"2000","journal-title":"Trends Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ijantimicag.2012.04.021","article-title":"A novel resistance mechanism to triclosan that suggests horizontal gene transfer and demonstrates a potential selective pressure for reduced biocide susceptibility in clinical strains of <italic>Staphylococcus aureus<\/italic>","volume":"40","author":"ML Ciusa","year":"2012","journal-title":"Int J Antimicrob Agents"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/ismej.2008.98","article-title":"Gene cassettes encoding resistance to quaternary ammonium compounds: a role in the origin of clinical class 1 integrons?","volume":"3","author":"MR Gillings","year":"2009","journal-title":"Isme J"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1038\/ismej.2011.15","article-title":"Impacts of anthropogenic activity on the ecology of class 1 integrons and integron-associated genes in the environment","volume":"5","author":"WH Gaze","year":"2011","journal-title":"Isme J"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/AAC.00498-13","article-title":"Evaluation of Reduced Susceptibility to Quaternary Ammonium Compounds and Bisbiguanides in Clinical Isolates and Laboratory-Generated Mutants of <italic>Staphylococcus aureus<\/italic>","volume":"57","author":"L Furi","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1089\/mdr.2012.0154","article-title":"The role of the <italic>qacA<\/italic> gene in mediating resistance to quaternary ammonium compounds","volume":"19","author":"D Cervinkova","year":"2013","journal-title":"Microb Drug Resist"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4159","DOI":"10.1128\/AAC.00257-10","article-title":"Genetic determinants involved in the susceptibility of <italic>Pseudomonas aeruginosa<\/italic> to beta-lactam antibiotics","volume":"54","author":"C Alvarez-Ortega","year":"2010","journal-title":"Antimicrob Agents Chemother"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1128\/AAC.00155-11","article-title":"Emergence of quinolone resistance and cephalosporin MIC creep in Neisseria gonorrhoeae isolates from a cohort of young men in Kisumu, Kenya, 2002 to 2009","volume":"55","author":"SD Mehta","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jinf.2009.11.011","article-title":"Vancomycin MIC creep in MRSA isolates from 1997 to 2008 in a healthcare region in Hong Kong","volume":"60","author":"PL Ho","year":"2010","journal-title":"J Infect"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.coph.2011.07.005","article-title":"Beyond serial passages: new methods for predicting the emergence of resistance to novel antibiotics","volume":"11","author":"JL Martinez","year":"2011","journal-title":"Curr Opin Pharmacol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/nrmicro1796","article-title":"Predicting antibiotic resistance","volume":"5","author":"JL Martinez","year":"2007","journal-title":"Nature Reviews in Microbiology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1574-6976.2008.00142.x","article-title":"A global view of antibiotic resistance","volume":"33","author":"JL Martinez","year":"2009","journal-title":"FEMS Microbiology Reviews"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1758-2229.2009.00053.x","article-title":"Ecology and evolution of antibiotic resistance","volume":"1","author":"F Baquero","year":"2009","journal-title":"Environ Microbiol Reports"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Oggioni MR, Ciusa ML, Furi L, Baldassarri L, Orefici G, <etal>et al<\/etal>.. (2012) Lack of evidence for reduced fitness of clinical Staphylococcus aureus isolates with reduced susceptibility to triclosan. Antimicrob Agents Chemother 56: 6068\u20136069; author reply 6072.","DOI":"10.1128\/AAC.01055-12"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"3092","DOI":"10.1128\/AAC.05904-11","article-title":"Attenuated virulence and biofilm formation in <italic>Staphylococcus aureus<\/italic> following sublethal exposure to triclosan","volume":"56","author":"J Latimer","year":"2012","journal-title":"Antimicrob Agents Chemother"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0086669","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T23:58:25Z","timestamp":1565135905000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0086669"}},"subtitle":[],"editor":[{"given":"Riccardo","family":"Manganelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,1,23]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,1,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0086669","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,23]]}}}