{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:59:47Z","timestamp":1761541187708},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0087089","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T21:44:15Z","timestamp":1390859055000},"page":"e87089","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evaluating the Synergistic Neutralizing Effect of Anti-Botulinum Oligoclonal Antibody Preparations"],"prefix":"10.1371","volume":"9","author":[{"given":"Eran","family":"Diamant","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bat-El","family":"Lachmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adi","family":"Keren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ada","family":"Barnea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadar","family":"Marcus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoshana","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alon Ben","family":"David","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Zichel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,1,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1001\/jama.285.8.1059","article-title":"Botulinum toxin as a biological weapon: medical and public health managment","volume":"285","author":"SS Arnon","year":"2001","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/MMBR.46.1.86-94.1982","article-title":"Bacterial toxins: a table of lethal amounts","volume":"46","author":"DM Gill","year":"1982","journal-title":"Microbiol Rev"},{"key":"ref3","first-page":"219","article-title":"Structure, activity, and immune (T and B cell) recognition of botulinum neurotoxins","volume":"19","author":"MZ Atassi","year":"1999","journal-title":"Crit Rev Immunol"},{"key":"ref4","unstructured":"Sugiyama H (1980) <italic>Clostridium botulinum<\/italic> neurotoxin. Microbiol Rev 44."},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Hatheway CL (1988) Botulism. In: Balows L, Hausler-Jr WJ, Ohashi M, Turano A, editors. Laboratory diagnosis of infectious diseases Principles and practice Volume I: bacterial, mycotic, and parasitic diseases: Springer-Verlag. 111\u2013133.","DOI":"10.1007\/978-1-4612-3898-0_12"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1006\/jmbi.1999.2945","article-title":"Sequence homology and structural analysis of the clostridial neurotoxins","volume":"291","author":"DB Lacy","year":"1999","journal-title":"J Mol Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/BF01886751","article-title":"Mapping of the antibody-binding regions of botulinum neorotoxin H-chain domain 855 to 1296 with anti-toxin antibodies from three host species","volume":"15","author":"MZ Atassi","year":"1996","journal-title":"J Prot Chem"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1128\/CVI.00496-09","article-title":"Efficacy of a potential trivalent vaccine based on Hc fragments of botulinum toxins A, B, and E produced in a cell-free expression system","volume":"17","author":"R Zichel","year":"2010","journal-title":"Clin Vaccine Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1128\/IAI.01893-06","article-title":"Trivalent vaccine against botulinum toxin serotypes A, B, and E that can be administered by the mucosal route","volume":"75","author":"E Ravichandran","year":"2007","journal-title":"Infect Immun"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.1128\/IAI.63.7.2738-2742.1995","article-title":"Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in <italic>Escherichia coli<\/italic>","volume":"63","author":"MA Clayton","year":"1995","journal-title":"Infect Immun"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1097\/DMP.0b013e318158c5fd","article-title":"Botulism: cause, effects, diagnosis, clinical and laboratory identification, and treatment modalities","volume":"1","author":"FZ Dembek","year":"2007","journal-title":"Disaster Med Public Health Prep"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0002-9343(80)90469-6","article-title":"Hypersensitivity reactions associated with botulinal antitoxin","volume":"69","author":"RE Black","year":"1980","journal-title":"Am J Med"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ab.2011.09.030","article-title":"Domain-based assays of individual antibody concentrations in an oligoclonal combination targeting a single protein","volume":"421","author":"Q Meng","year":"2012","journal-title":"Anal Biochem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/CMR.19.2.298-314.2006","article-title":"Laboratory diagnostics of botulism","volume":"19","author":"M Lindstrom","year":"2006","journal-title":"Clin Microbiol Rev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1007\/s10529-008-9706-5","article-title":"Mouse monoclonal antibodies in biological research: strategies for high-throughput production","volume":"30","author":"P Chiarella","year":"2008","journal-title":"Biotechnol Lett"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1128\/AEM.50.1.63-67.1985","article-title":"Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay","volume":"50","author":"C Shone","year":"1985","journal-title":"Appl Environ Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jim.2005.04.003","article-title":"Comparison of electrochemiluminescence assay and ELISA for the detection of Clostridium botulinum type B neurotoxin","volume":"301","author":"V Guglielmo Viret","year":"2005","journal-title":"J Immunol Methods"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"7897","DOI":"10.1128\/AEM.71.12.7897-7903.2005","article-title":"Sensitive detection of Botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test","volume":"71","author":"F Gessler","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2008.03.003","article-title":"Development and partial characterization of high-affinity monoclonal antibodies for botulinum toxin type A and their use in analysis of milk by sandwich ELISA","volume":"336","author":"LH Stanker","year":"2008","journal-title":"J Immunol Meth"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ab.2012.02.038","article-title":"A functional dual-coated (FDC) microtiter plate method to replace the botulinum toxin LD 50 test","volume":"425","author":"YYB Liu","year":"2012","journal-title":"Anal Biochem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"430","DOI":"10.3390\/toxins4060430","article-title":"Monoclonal antibodies and toxins - A perspective on function and isotype","volume":"4","author":"S-K Chow","year":"2012","journal-title":"Toxins"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ab.2012.08.005","article-title":"Engineered domain-based assays to identify individual antibodies in oligoclonal combinations targeting the same protein","volume":"430","author":"Q Meng","year":"2012","journal-title":"Anal Biochem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"9153","DOI":"10.1016\/S0021-9258(19)38824-6","article-title":"The complete sequence of botulinum neurotoxin type A and comparison with other clostridial neurotoxins","volume":"265","author":"T Binz","year":"1990","journal-title":"J Biol Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/j.1432-1033.1992.tb16679.x","article-title":"The complete amino acid sequence of the <italic>Clostridium botulinum<\/italic> type E neurotoxin derived by sequence analysis of the encoding gene","volume":"204","author":"SM Whelan","year":"1992","journal-title":"Eur J Biochem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1128\/AEM.58.8.2345-2354.1992","article-title":"Molecular cloning of the <italic>Clostridium botulinum<\/italic> structural gene gene encoding the type B neurotoxin and determination of its entire nucleotide sequence","volume":"58","author":"SM Whelan","year":"1992","journal-title":"Appl Environ Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/266550a0","article-title":"Antibodies to major histocompatibility antigens produced by hybrid cell lines","volume":"296","author":"G Galfre","year":"1977","journal-title":"Nature"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"296","author":"G Kohler","year":"1975","journal-title":"Nature"},{"key":"ref28","unstructured":"EP (2011) Botulinum antitoxin. European Pharmacopoeia. 949."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"4305","DOI":"10.1128\/IAI.00405-09","article-title":"Antibody protection against botulinum neurotoxin intoxication in mice","volume":"77","author":"LW Cheng","year":"2009","journal-title":"Infect Immun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"11346","DOI":"10.1073\/pnas.172229899","article-title":"Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody","volume":"99","author":"A Nowakowski","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref31","first-page":"299","article-title":"Investigational heptavalent botulinum antitoxin (HBAT) to replace licensed botulinum antitoxin AB and investigational botulinum antitoxin E. MMWR Morb Mortal Wkly Rep","volume":"59","year":"2010"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"4070","DOI":"10.1002\/pmic.200401279","article-title":"High-throughput mouse monoclonal antibodies using antigen microarrays","volume":"5","author":"F De Masi","year":"2005","journal-title":"Proteomics"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1615\/CritRevImmunol.v27.i4.20","article-title":"Botulinum neurotoxin vaccines: Past, present, and future","volume":"27","author":"LA Smith","year":"2007","journal-title":"Crit Rev Immuno"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/btpr.564","article-title":"Comparison and critical analysis of robotized technology for monoclonal antibody high-throughput production","volume":"27","author":"P Chiarella","year":"2011","journal-title":"Biotechnol Prog"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jim.2007.08.017","article-title":"High-throughput homogeneous immunoassay readily identifies monoclonal antibody to serovariant clostridial neurotoxins","volume":"328","author":"CR Corbett","year":"2007","journal-title":"J Immunol Methods"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/eye.1988.5","article-title":"Clostridium botulinum toxins: nature and preparation for clinical use","volume":"2","author":"J Melling","year":"1988","journal-title":"Eye"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/protein\/gzq111","article-title":"Neutralizing human monoclonal antibodies binding multiple serotypes of botulinum neurotoxin","volume":"24","author":"C Garcia-Rodriguez","year":"2011","journal-title":"Protein Eng Des Sel"},{"key":"ref38","first-page":"570","article-title":"High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. Infect Immun","volume":"69","author":"DD Pless","year":"2001"},{"key":"ref39","first-page":"307","article-title":"Monoclonal antibodies to botulinum neurotoxins of types A, B, E, and F. Russ J Bioorganic Chem","volume":"37","author":"SG Abbasova","year":"2011"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1089\/hyb.1997.16.447","article-title":"Identification and characterization of a neutralizing monoclonal antibody against botulinum neurotoxin, serotype F, following vaccination with active toxin","volume":"16","author":"DR Brown","year":"1997","journal-title":"Hybridoma"},{"key":"ref41","first-page":"19","article-title":"Isolation and characterization of a neutralizing antibody specific to internalization domain of <italic>Clostridium botulinum<\/italic> neurotoxin type B. Toxicon","volume":"44","author":"G Yang","year":"2004"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nbt0602-597","article-title":"Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity","volume":"20","author":"JA Maynard","year":"2002","journal-title":"Nat Biotechnol"},{"key":"ref43","first-page":"1315","article-title":"Development and preclinical evaluation of a new F(ab\u2019)<sub>2<\/sub> antitoxin against botulinum neurotoxin serotype A. Biochimie","volume":"92","author":"YZ Yu","year":"2010"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Prigent J, Mazuet C, Boquet D, Lamourette P, Volland H, <etal>et al<\/etal>.. (2010) Production and characterisation of a neutralising chimeric antibody against botulinum neurotoxin A. PLoS One 5.","DOI":"10.1371\/journal.pone.0013245"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"5450","DOI":"10.1128\/IAI.73.9.5450-5457.2005","article-title":"Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization","volume":"73","author":"TJ Smith","year":"2005","journal-title":"Infect Immun"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"138","DOI":"10.7883\/yoken.65.138","article-title":"Characterization of the monoclonal antibody response to botulinum neurotoxin type A in the complexed and uncomplexed forms","volume":"65","author":"H Zhao","year":"2012","journal-title":"Jpn J Infect Dis"},{"key":"ref47","first-page":"120","article-title":"A sensitive sandwich enzyme immunoassay for free or complexed Clostridium botulinum neurotoxin type A. J Immunol Methods","volume":"330","author":"H Volland","year":"2008"},{"key":"ref48","doi-asserted-by":"crossref","unstructured":"Mazor O, Ben-Arie E, Mechaly A, Rosenfeld R, Marcus H, <etal>et al<\/etal>.. (2010) Combination of anthrax-toxin neutralizing antibodies: analysis of synergism\/antagonism effect. In: Shafferman A, Ordentlich A, Velan B, editors. The challenge of highly pathogenic microorganisms: mechanism of virulence and novel medical countermeasures: Springer.","DOI":"10.1007\/978-90-481-9054-6_30"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1128\/IAI.45.3.604-609.1984","article-title":"Neutralization of tetanus toxin by distinct monoclonal antibodies binding to multiple epitopes on the toxin molecule","volume":"45","author":"WA Volk","year":"1984","journal-title":"Infect Immun"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"12198","DOI":"10.1128\/JVI.75.24.12198-12208.2001","article-title":"Neutralization synergy of human immunodeficiency virus type 1 primary isolates by cocktails of broadly neutralizing antibodies","volume":"75","author":"MB Zwick","year":"2001","journal-title":"J Virol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1016\/j.chroma.2010.10.107","article-title":"Separation of oxidized variants of a monoclonal antibody by anion-exchange","volume":"1218","author":"G Teshima","year":"2011","journal-title":"J Chromatogr A"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1002\/art.10189","article-title":"Defective Fc-dependent processing of immune complexes in patients with systemic lupus erythematosus","volume":"46","author":"KA Davies","year":"2002","journal-title":"Arthritis Rheum"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"3255","DOI":"10.1242\/jcs.113.18.3255","article-title":"Fc receptor mediated endocytosis of small soluble immunoglobulin G immune compexes in Kupffer and endothelial cells from rat liver","volume":"113","author":"T Lovdal","year":"2000","journal-title":"J Cell Sci"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"e29941","DOI":"10.1371\/journal.pone.0029941","article-title":"A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model","volume":"7(1)","author":"J Mukherjee","year":"2012","journal-title":"PLoS One"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0087089","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T02:44:15Z","timestamp":1688957055000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0087089"}},"subtitle":[],"editor":[{"given":"Ellen R.","family":"Goldman","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,1,27]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,1,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0087089","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,27]]}}}