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Fisheries","volume":"18","author":"MK Litvak","year":"1993"},{"key":"ref2","unstructured":"USDA (United States Department of Agriculture) (2006) Census of Aquaculture (2005). 2002 Census of Agriculture: Vol. 3 Special Studies Part 2. AC-02-SP-2. United States."},{"key":"ref3","unstructured":"Gunderson JL, Tucker P (2000) A white paper on the status and needs of baitfish aquaculture in the north central region. United States Department of Agriculture, North Central Regional Aquaculture Center. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.ncrac.org\/content\/status-and-needs-baitfish-aquaculture-north-central-region\" xlink:type=\"simple\">http:\/\/www.ncrac.org\/content\/status-and-needs-baitfish-aquaculture-north-central-region<\/ext-link> Accessed 16 October 2013."},{"key":"ref4","unstructured":"Stone N, Thomforde H (2001) Common farmed-raised baitfish. United States Department of Agriculture, Southern Regional Aquaculture Center. Publication No. 120. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/srac.tamu.edu\/index.cfm\/event\/getFactSheet\/whichfactsheet\/7\" xlink:type=\"simple\">https:\/\/srac.tamu.edu\/index.cfm\/event\/getFactSheet\/whichfactsheet\/7<\/ext-link> Accessed 16 October 2013."},{"key":"ref5","first-page":"19","article-title":"Transmission of exotic fish viruses: the relative risks of wild and cultured bait. Fisheries","volume":"29","author":"AE Goodwin","year":"2004"},{"key":"ref6","first-page":"523","article-title":"Hedrick RP. 1996. Movements of pathogens with the international trade of live fish: problems and solutions. Rev Sci Tech Off Int Epizoot","volume":"15"},{"key":"ref7","first-page":"1505","article-title":"Emerging marine diseases \u2013 Climate links anthropogenic factors. Science","volume":"285","author":"CD Harvell","year":"1999"},{"key":"ref8","first-page":"113","article-title":"Disease-translocation across geographic boundaries must be recognized as a risk even in the absence of disease identification: the case with Australian <italic>Sardinops<\/italic>. Rev Fish Biol Fish","volume":"11","author":"DJ Gaughan","year":"2002"},{"key":"ref9","unstructured":"USDA (United States Department of Agriculture) (2008) Federal Order to prevent the spread of VHSV. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.aphis.usda.gov\/animal_health\/animal_dis_spec\/aquaculture\/downloads\/vhs_fed_order_amended.pdf\" xlink:type=\"simple\">http:\/\/www.aphis.usda.gov\/animal_health\/animal_dis_spec\/aquaculture\/downloads\/vhs_fed_order_amended.pdf<\/ext-link> Accessed 16 October 2013."},{"key":"ref10","first-page":"9","article-title":"Emergence and resurgence of the viral hemorrhagic septicemia virus (<italic>Novirhabdovirus<\/italic>, <italic>Rhabdoviridae<\/italic>, <italic>Mononegavirales<\/italic>). J Adv Res","volume":"2","author":"R Kim","year":"2011"},{"key":"ref11","unstructured":"USFWS and AFS-FHS (U.S. Fish and Wildlife Service and American Fisheries Society-Fish Health Section) (2010) Standard procedures for aquatic animal health inspections. In: AFS-FHS, FHS blue book: suggested procedures for the detection and identification of certain finfish and shellfish pathogens, ed. Bethesda, Maryland: AFS-FHS."},{"key":"ref12","unstructured":"OIE (World Organization for Animal Health) (2011) Manual of diagnostic tests for aquatic animals, 7<sup>th<\/sup> ed. Paris, France: World Organization for Animal Health."},{"key":"ref13","unstructured":"McCann R (2012) Viral survey of fathead minnows, golden shiners, and white suckers from baitfish dealers in Wisconsin. M.S. thesis. University of Wisconsin, La Crosse, WI."},{"key":"ref14","unstructured":"Knowles NJ, Hovi T, Hyypi\u00e4 T, King AMQ, Lindberg AM, et al. (2012) Picornaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ, editors. Virus Taxonomy: Classification and Nomenclature of Viruses, Ninth Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier. 855\u2013880."},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Adams MJ, King AMQ, Carstens EB (2013) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). Arch Virol. doi:<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1007\/s00705-013-1688-5\" xlink:type=\"simple\">10.1007\/s00705-013-1688-5<\/ext-link>.","DOI":"10.1007\/s00705-013-1688-5"},{"key":"ref16","unstructured":"Racaniello VR (2007) Picornaviridae: the viruses and their replication. In: Knipe DM, Howley PM, Griffin DE, editors. Fields Virology, 5<sup>th<\/sup> edition. Philadelphia, PA: Williams &amp; Wilkins. 795\u2013838."},{"key":"ref17","first-page":"65","article-title":"Encephalomyelitis among turbot associated with a picornavirus-like agent. Dis Aquat Org","volume":"10","author":"B Bloch","year":"1991"},{"key":"ref18","first-page":"99","article-title":"A small RNA virus isolated from salmonid fishes in California, USA. Can J Fish Aquat Sci","volume":"48","author":"RP Hedrick","year":"1991"},{"key":"ref19","first-page":"349","article-title":"A small RNA virus isolated from apparently healthy wild sandbar shiners, <italic>Notropis scepticus<\/italic> (Jordan &amp; Gilbert). J Fish Dis","volume":"23","author":"LR Iwanowicz","year":"2000"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Fichtner D, Philipps A, Groth M, Schmidt-Posthaus H, Granzow H, et al. (2013) Characterization of a novel picornavirus isolate from a diseased European eel (<italic>Anguilla anguilla<\/italic>). J Virol. doi:<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1128\/JVI.01094-13\" xlink:type=\"simple\">10.1128\/JVI.01094-13<\/ext-link>.","DOI":"10.1128\/JVI.01094-13"},{"key":"ref21","first-page":"368","article-title":"Properties of a new virus belonging to nodaviridae found in larval striped jack (<italic>Pseudocarnax dentex<\/italic>) with nervous necrosis. Vir","volume":"187","author":"KI Mori","year":"1992"},{"key":"ref22","first-page":"116","article-title":"A novel member of the family <italic>Hepeviridae<\/italic> from cutthroat trout (<italic>Oncorhynchus clarkii<\/italic>). Virus Res","volume":"158","author":"W Batts","year":"2011"},{"key":"ref23","unstructured":"Barbknecht M (2009) Characterization of an unclassified virus and survey for its presence in Wisconsin bluegill populations. M.S. thesis. University of Wisconsin, La Crosse, WI."},{"key":"ref24","first-page":"257","article-title":"Molecular, physical, and clinical evidence that golden shiner virus and grass carp reovirus are variants of the same virus. J Aquat Anim Health","volume":"15","author":"ME McEntire","year":"2003"},{"key":"ref25","first-page":"1247","article-title":"Genetic analysis of a novel nidovirus from fathead minnows. J Gen Virol","volume":"93","author":"WN Batts","year":"2012"},{"key":"ref26","first-page":"18","article-title":"Universal virus detection by degenerate-oligonucleotide primed polymerase chain reaction of purified viral nucleic acids. J Virol Methods","volume":"152","author":"S Nanda","year":"2008"},{"key":"ref27","first-page":"311","article-title":"A highly divergent picornavirus in a marine mammal. J Virol","volume":"82","author":"A Kapoor","year":"2008"},{"key":"ref28","first-page":"4642","article-title":"Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. J Virol","volume":"83","author":"JG Victoria","year":"2009"},{"key":"ref29","first-page":"12161","article-title":"High variety of known and new RNA and DNA viruses of diverse origins in untreated sewage. J Virol","volume":"86","author":"TF Ng","year":"2012"},{"key":"ref30","first-page":"2203","article-title":"Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks. Protein Sci","volume":"5","author":"N Blom","year":"1996"},{"key":"ref31","first-page":"4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res","volume":"22","author":"JD Thompson","year":"1994"},{"key":"ref32","first-page":"2731","article-title":"MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol","volume":"28","author":"K Tamura","year":"2011"},{"key":"ref33","first-page":"2104","article-title":"ProtTest: selection of best-fit models of protein evolution. Bioinformatics","volume":"21","author":"F Abascal","year":"2005"},{"key":"ref34","first-page":"1","article-title":"Phylemon 2.0: a suite of web-tools for molecular evolution, phylogenetics, phylogenomics and hypotheses testing. Nucleic Acids Res","volume":"39","author":"R Sanchez","year":"2011"},{"key":"ref35","first-page":"275","article-title":"The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci","volume":"8","author":"DT Jones","year":"1992"},{"key":"ref36","first-page":"783","article-title":"Confidence limits on phylogenies: an approach using bootstrap. Evolution","volume":"39","author":"J Felsenstein","year":"1985"},{"key":"ref37","unstructured":"Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, et al. (2011) Geneious v5.4. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.geneious.com\" xlink:type=\"simple\">http:\/\/www.geneious.com<\/ext-link> Accessed 16 October 2013."},{"key":"ref38","first-page":"1411","article-title":"A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae). Arch Virol","volume":"158","author":"B Muhire","year":"2013"},{"key":"ref39","first-page":"925","article-title":"Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 17: 4713\u20134730. Koonin EV, Wolf YI, Nagasaki K, Dolja VV (2008) The big bang of picorna-like virus evolution antedates the radiation of eukaryotic supergroups. Nat Rev Micro","volume":"6","author":"AE Gorbalenya","year":"1989"},{"key":"ref40","first-page":"190","article-title":"Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. Virus Res","volume":"123","author":"CH Tseng","year":"2007"},{"key":"ref41","first-page":"10036","article-title":"A member of a new Picornaviridae genus is shed in pig feces. J Virol","volume":"86","author":"V Sauvage","year":"2012"},{"key":"ref42","first-page":"8232","article-title":"Molecular and biological characteristics of echovirus 22, a representative of an new picornavirus group. J Virol","volume":"68","author":"G Stanway","year":"1994"},{"key":"ref43","first-page":"201","article-title":"The 2A proteins of three diverse picornaviruses are related to each other and to the H-rev107 family of proteins involved in the control of cell proliferation. J Gen Virol","volume":"81","author":"PJ Hughes","year":"2000"},{"key":"ref44","first-page":"5263","article-title":"Protein is linked to the 5\u2032 end of poliovirus RNA by a phosphodiester linkage to tyrosine. J Biol Chem","volume":"253","author":"V Ambros","year":"1978"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0087593","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T03:41:34Z","timestamp":1540006894000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0087593"}},"subtitle":[],"editor":[{"given":"Amit","family":"Kapoor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,2,21]]},"references-count":44,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,2,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0087593","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,21]]}}}