{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:55:43Z","timestamp":1790873743081,"version":"4.1.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T00:00:00Z","timestamp":1393459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0090005","type":"journal-article","created":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T23:18:57Z","timestamp":1393543137000},"page":"e90005","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":106,"title":["In Vivo Activity of MiR-34a Mimics Delivered by Stable Nucleic Acid Lipid Particles (SNALPs) against Multiple Myeloma"],"prefix":"10.1371","volume":"9","author":[{"given":"Maria Teresa","family":"Di Martino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Campani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Misso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eugenia","family":"Gallo Cantafio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annamaria","family":"Gull\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umberto","family":"Foresta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro Hiram","family":"Guzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Castellano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Grimaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Gigantino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Lusa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Cannataro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierosandro","family":"Tagliaferri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"De Rosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierfrancesco","family":"Tassone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Caraglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,2,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.2217\/14796694.3.5.521","article-title":"miRNAs and their potential for use against cancer and other diseases","volume":"3","author":"E Tili","year":"2007","journal-title":"Future Oncol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.1038\/onc.2009.353","article-title":"MicroRNAs as potential cancer therapeutics","volume":"27","author":"P Trang","year":"2008","journal-title":"Oncogene"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1038\/nature03315","article-title":"Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs","volume":"433","author":"LP Lim","year":"2005","journal-title":"Nature"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1016\/j.bbrc.2006.12.135","article-title":"Instability of miRNA and cDNAs derivatives in RNA preparations","volume":"353","author":"V Bravo","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1517\/17425247.2010.527942","article-title":"Oligonucleotide delivery in cancer therapy","volume":"7","author":"G De Rosa","year":"2010","journal-title":"Expert Opin Drug Deliv"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0005-2736(00)00343-6","article-title":"Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures","volume":"1510","author":"SC Semple","year":"2001","journal-title":"Biochim Biophys Acta"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nature04688","article-title":"RNAi-mediated gene silencing in non-human primates","volume":"441","author":"TS Zimmermann","year":"2006","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/cdd.2009.56","article-title":"The miR-34 family in cancer and apoptosis","volume":"17","author":"H Hermeking","year":"2010","journal-title":"Cell Death Differ"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1016\/j.cub.2007.06.068","article-title":"p53-mediated activation of miRNA34 candidate tumor-suppressor genes","volume":"17","author":"GT Bommer","year":"2007","journal-title":"Curr Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.molcel.2007.05.010","article-title":"Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis","volume":"26","author":"TC Chang","year":"2007","journal-title":"Mol Cell"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1038\/nature05939","article-title":"A microRNA component of the p53 tumour suppressor network","volume":"447","author":"L He","year":"2007","journal-title":"Nature"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.4161\/cc.6.13.4436","article-title":"Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrest","volume":"6","author":"V Tarasov","year":"2007","journal-title":"Cell Cycle"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"15472","DOI":"10.1073\/pnas.0707351104","article-title":"Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells","volume":"104","author":"H Tazawa","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.molcel.2007.05.017","article-title":"Transcriptional activation of miR-34a contributes to p53-mediated apoptosis","volume":"26","author":"N Raver-Shapira","year":"2007","journal-title":"Mol Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1093\/carcin\/bgq033","article-title":"Epigenetic inactivation of the miR-34a in hematological malignancies","volume":"31","author":"CS Chim","year":"2010","journal-title":"Carcinogenesis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e6816","DOI":"10.1371\/journal.pone.0006816","article-title":"MicroRNA miR-34 inhibits human pancreatic cancer tumor-initiating cells","volume":"4","author":"Q Ji","year":"2009","journal-title":"PLoS One"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/nm.2284","article-title":"The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44","volume":"17","author":"C Liu","year":"2011","journal-title":"Nat Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"5923","DOI":"10.1158\/0008-5472.CAN-10-0655","article-title":"Development of a lung cancer therapeutic based on the tumor suppressor microRNA-34","volume":"70","author":"JF Wiggins","year":"2010","journal-title":"Cancer Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2174\/156800912802429300","article-title":"Molecular targets for the treatment of multiple myeloma","volume":"12","author":"M Rossi","year":"2012","journal-title":"Curr Cancer Drug Targets"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"823","DOI":"10.2174\/156800912802429274","article-title":"MicroRNAs in the pathobiology of multiple myeloma","volume":"12","author":"M Lionetti","year":"2012","journal-title":"Curr Cancer Drug Targets"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.18632\/oncotarget.675","article-title":"DNA-demethylating and anti-tumor activity of synthetic miR-29b mimics in multiple myeloma","volume":"3","author":"N Amodio","year":"2012","journal-title":"Oncotarget"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e436","DOI":"10.1038\/cddis.2012.175","article-title":"miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1","volume":"3","author":"N Amodio","year":"2012","journal-title":"Cell Death Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1158\/1078-0432.CCR-12-3325","article-title":"Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growth","volume":"19","author":"E Leone","year":"2013","journal-title":"Clin Cancer Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1002\/jcp.24306","article-title":"miR-29b negatively regulates human osteoclastic cell differentiation and function: implications for the treatment of multiple myeloma-related bone disease","volume":"228","author":"M Rossi","year":"2013","journal-title":"J Cell Physiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"242","DOI":"10.18632\/oncotarget.820","article-title":"In vitro and in vivo anti-tumor activity of miR-221\/222 inhibitors in multiple myeloma","volume":"4","author":"MT Di Martino","year":"2013","journal-title":"Oncotarget"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Amodio N, Bellizzi D, Leotta M, Raimondi L, Biamonte L, <etal>et al<\/etal>.. (2013) miR-29b induces SOCS-1 expression by promoter demethylation and negatively regulates migration of multiple myeloma and endothelial cells. Cell Cycle 12.","DOI":"10.4161\/cc.26585"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"6260","DOI":"10.1158\/1078-0432.CCR-12-1708","article-title":"Synthetic miR-34a mimics as a novel therapeutic agent for multiple myeloma: in vitro and in vivo evidence","volume":"18","author":"MT Di Martino","year":"2012","journal-title":"Clin Cancer Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e24584","DOI":"10.1371\/journal.pone.0024584","article-title":"MiR-34a targeting of Notch ligand delta-like 1 impairs CD15+\/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma","volume":"6","author":"P de Antonellis","year":"2011","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"838","DOI":"10.2174\/156800912802429355","article-title":"Promises and challenges of MicroRNA-based treatment of multiple myeloma","volume":"12","author":"P Tagliaferri","year":"2012","journal-title":"Curr Cancer Drug Targets"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/0003-2697(80)90269-9","article-title":"Colorimetric determination of phospholipids with ammonium ferrothiocyanate","volume":"104","author":"JC Stewart","year":"1980","journal-title":"Anal Biochem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1073\/pnas.98.1.31","article-title":"Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection","volume":"98","author":"C Li","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1111\/j.1365-2141.2008.07387.x","article-title":"In vivo anti-myeloma activity and modulation of gene expression profile induced by valproic acid, a histone deacetylase inhibitor","volume":"143","author":"P Neri","year":"2008","journal-title":"Br J Haematol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S0092-8674(03)01018-3","article-title":"Prediction of mammalian microRNA targets","volume":"115","author":"BP Lewis","year":"2003","journal-title":"Cell"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"W Huang da","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3771","DOI":"10.1158\/1078-0432.CCR-06-2921","article-title":"Targeting the phosphatidylinositol 3-kinase pathway in multiple myeloma","volume":"13","author":"H Younes","year":"2007","journal-title":"Clin Cancer Res"},{"key":"ref37","first-page":"6763","article-title":"The phosphatidylinositol 3-kinase\/AKT kinase pathway in multiple myeloma plasma cells: roles in cytokine-dependent survival and proliferative responses","volume":"60","author":"Y Tu","year":"2000","journal-title":"Cancer Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"814","DOI":"10.2174\/156800912802429292","article-title":"Mouse models as a translational platform for the development of new therapeutic agents in multiple myeloma","volume":"12","author":"P Tassone","year":"2012","journal-title":"Curr Cancer Drug Targets"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/leu.2010.300","article-title":"A unique three-dimensional SCID-polymeric scaffold (SCID-synth-hu) model for in vivo expansion of human primary multiple myeloma cells","volume":"25","author":"T Calimeri","year":"2011","journal-title":"Leukemia"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1182\/blood-2004-11-4477","article-title":"A SCID-hu in vivo model of human Waldenstrom macroglobulinemia","volume":"106","author":"P Tassone","year":"2005","journal-title":"Blood"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1182\/blood-2005-01-0373","article-title":"A clinically relevant SCID-hu in vivo model of human multiple myeloma","volume":"106","author":"P Tassone","year":"2005","journal-title":"Blood"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/nrclinonc.2012.15","article-title":"Latest advances and current challenges in the treatment of multiple myeloma","volume":"9","author":"A Mahindra","year":"2012","journal-title":"Nat Rev Clin Oncol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.2174\/13894501113149990186","article-title":"From target therapy to miRNA therapeutics of human multiple myeloma: theoretical and technological issues in the evolving scenario","volume":"14","author":"M Rossi","year":"2013","journal-title":"Curr Drug Targets"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"S125","DOI":"10.1517\/14712598.2013.796356","article-title":"Non-coding RNA: a novel opportunity for the personalized treatment of multiple myeloma","volume":"13","author":"N Amodio","year":"2013","journal-title":"Expert Opin Biol Ther"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"e1002363","DOI":"10.1371\/journal.pgen.1002363","article-title":"Capture of microRNA-bound mRNAs identifies the tumor suppressor miR-34a as a regulator of growth factor signaling","volume":"7","author":"A Lal","year":"2011","journal-title":"PLoS Genet"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s10549-011-1690-0","article-title":"Identification of the receptor tyrosine kinase AXL in breast cancer as a target for the human miR-34a microRNA","volume":"130","author":"M Mackiewicz","year":"2011","journal-title":"Breast Cancer Res Treat"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jconrel.2012.04.038","article-title":"Macromolecular therapeutics in cancer treatment: the EPR effect and beyond","volume":"164","author":"H Maeda","year":"2012","journal-title":"J Control Release"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1172\/JCI37515","article-title":"Confirming the RNAi-mediated mechanism of action of siRNA-based cancer therapeutics in mice","volume":"119","author":"AD Judge","year":"2009","journal-title":"J Clin Invest"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.1038\/onc.2011.126","article-title":"Molecular targeting of CSN5 in human hepatocellular carcinoma: a mechanism of therapeutic response","volume":"30","author":"YH Lee","year":"2011","journal-title":"Oncogene"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0090005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T17:37:34Z","timestamp":1716572254000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0090005"}},"subtitle":[],"editor":[{"given":"Gerolama","family":"Condorelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,2,27]]},"references-count":49,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,2,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0090005","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,27]]}}}