{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:06:32Z","timestamp":1785294392542,"version":"3.55.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2014,3,19]],"date-time":"2014-03-19T00:00:00Z","timestamp":1395187200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0091800","type":"journal-article","created":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T02:49:24Z","timestamp":1395283764000},"page":"e91800","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":63,"title":["Detection of OXA-1 \u03b2-Lactamase Gene of Klebsiella pneumoniae from Blood Stream Infections (BSI) by Conventional PCR and In-Silico Analysis to Understand the Mechanism of OXA Mediated Resistance"],"prefix":"10.1371","volume":"9","author":[{"given":"Madhan","family":"Sugumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. M.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Manoharan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Anbarasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudha","family":"Ramaiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,3,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1128\/AAC.16.4.427","article-title":"\u03b2-Lactam resistance in clinical isolates of <italic>Escherichia coli<\/italic> caused by elevated production of the ampC-mediated chromosomal \u03b2-lactamase","volume":"16","author":"S Bergstrom","year":"1979","journal-title":"Antimicrob Agents Chemother"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1574-6968.1990.tb04040.x","article-title":"Hyperproduction of TEM-1 \u03b2-lactamase in clinical isolates of <italic>Escherichia coli<\/italic> serotype O15","volume":"67","author":"K Shannon","year":"1990","journal-title":"FEMS Microbiol Lett"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1128\/AAC.38.5.1085","article-title":"Emergence of clinical isolates of <italic>Escherichia coli<\/italic> producing TEM-1derivatives or an OXA-1 \u03b2-lactamase conferring resistance to \u03b2-lactamase inhibitors","volume":"38","author":"XY Zhou","year":"1994","journal-title":"Antimicrob Agents Chemother"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1128\/AAC.01247-10","article-title":"Detection of NDM-1- producing <italic>Klebsiella pneumoniae<\/italic> in Kenya","volume":"55","author":"L Poirel","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"161","DOI":"10.4103\/0255-0857.81799","article-title":"Correlation of TEM, SHV and CTX-M extended-spectrum \u03b2-lactamases among <italic>Enterobacteriaceae<\/italic> with their <italic>in vitro<\/italic> antimicrobial susceptibility","volume":"29","author":"A Manoharan","year":"2011","journal-title":"Indian Journal of Medical Microbiology"},{"key":"ref6","first-page":"31","article-title":"Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial susceptibility testing, Twenty-First Informational Supplement","volume":"M100-S21","author":"PA Wayne","year":"2011","journal-title":"CLSI document"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/jac\/dkf069","article-title":"Identification of PSE and OXA \u03b2-lactamase genes in <italic>Pseudomonas aeruginosa<\/italic> using PCR-restriction fragment length polymorphism","volume":"50","author":"B Frederic","year":"2002","journal-title":"J Antimicrob Chemother"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"D193","DOI":"10.1093\/nar\/gkl929","article-title":"The universal protein resource (UniProt)","volume":"35","author":"A Bairoch","year":"2007","journal-title":"Nucleic Acids research"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/jmbi.1993.1626","article-title":"Comparative protein modeling by satisfaction of spatial restraints","volume":"234","author":"A Sali","year":"1993","journal-title":"Journal of Molecular Biology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1002\/elps.1150181505","article-title":"SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling","volume":"18","author":"N Guex","year":"1997","journal-title":"Electrophorosis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program to check the stereochemical quality of protein structures","volume":"26","author":"RA Laskowski","year":"1993","journal-title":"Journal of Applied Crystallography"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1093\/bioinformatics\/bti315","article-title":"Q-Site Finder: an energy-based method for the prediction of protein-ligand binding sites","volume":"21","author":"A Laurie","year":"2005","journal-title":"Bioinformatics"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.2174\/156802607781696783","article-title":"Targeting protein multiple conformations: a Structure based strategy for kinase drug design","volume":"7","author":"JJ Liao","year":"2007","journal-title":"Curr Top Med Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1002\/(SICI)1096-987X(19981115)19:14<1639::AID-JCC10>3.0.CO;2-B","article-title":"Automated Docking Using a Lamarckian Genetic Algorithm and and Empirical Binding Free Energy Function","volume":"19","author":"GM Morris","year":"1998","journal-title":"J Comput Chem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H","article-title":"LINCS: A linear constraint solver for molecular simulations","volume":"18","author":"B Hess","year":"1997","journal-title":"Journal of Computational Chemistry"},{"key":"ref16","first-page":"1355","article-title":"PRODRG - a tool for high-throughput crystallography of protein-ligand complexes","volume":"D60","author":"W Schuttelkopf","year":"2004","journal-title":"Acta Crystallogr"},{"key":"ref17","unstructured":"Gunsteren WFV, Billeter SR, Eising AA, Hunenberger PH, Kruger PK, <etal>et al<\/etal>.. (1996) Biomolecular simulation: The GROMOS96 manual and user guide. Verlag der Fachvereine. Zurich: pp 1\u20131024."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1002\/jcc.20090","article-title":"A biomolecular force field based on the free enthalpy of hydration and solvation: The GROMOS force-field parameter sets 53A5 and 53A6","volume":"25","author":"C Oostenbrink","year":"2004","journal-title":"Journal of Computational Chemistry"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/CMR.00037-09","article-title":"Three Decades of \u03b2-Lactamase Inhibitors","volume":"23","author":"SM Drawz","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"3113","DOI":"10.1128\/JCM.37.10.3113-3117.1999","article-title":"Typing and characterization of mechanisms of resistance of <italic>Shigella<\/italic> spp isolated from feces of children under 5 years of age from Ifakara, Tanzania","volume":"37","author":"MM Navia","year":"1999","journal-title":"J Clin Microbiol"},{"key":"ref21","first-page":"2034","article-title":"\u03b2-lactamases in <italic>Shigella flexneri<\/italic> isolates from Hong Kong and Shangai and a novel OXA-1 like \u03b2-lactamase, OXA-30","author":"LK Siu","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0378-1097(03)00306-9","article-title":"Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in enterobactericeae","volume":"223","author":"C Karmele","year":"2003","journal-title":"FEMS Microbiol Lett"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0091800","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T14:06:39Z","timestamp":1597586799000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0091800"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,3,19]]},"references-count":22,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,3,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0091800","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,19]]}}}