{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:54:29Z","timestamp":1791417269487,"version":"4.3.3"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T00:00:00Z","timestamp":1395014400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0091971","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T20:49:27Z","timestamp":1395089367000},"page":"e91971","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Contribution of Eukaryotic-Type Serine\/Threonine Kinase to Stress Response and Virulence of Streptococcus suis"],"prefix":"10.1371","volume":"9","author":[{"given":"Haodan","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxiu","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libin","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixin","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongli","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kongwang","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.ijmm.2009.08.016","article-title":"The impact of serine\/threonine phosphorylation in Staphylococcus aureus","volume":"300","author":"K Ohlsen","year":"2010","journal-title":"Int J Med Microbiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev.biochem.69.1.183","article-title":"Two-component signal transduction","volume":"69","author":"AM Stock","year":"2000","journal-title":"Annu Rev Biochem"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/0092-8674(91)90372-6","article-title":"A gene encoding a protein serine\/threonine kinase is required for normal development of M. xanthus, a gram-negative bacterium","volume":"67","author":"J Munoz-Dorado","year":"1991","journal-title":"Cell"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1007\/s002530051602","article-title":"Phage abortive infection of Bacillus licheniformis ATCC 9800; identification of the abiBL11 gene and localisation and sequencing of its promoter region","volume":"52","author":"LS Tran","year":"1999","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.jmb.2006.01.020","article-title":"Identification and biochemical characterization of a eukaryotic-type serine\/threonine kinase and its cognate phosphatase in Streptococcus pyogenes: their biological functions and substrate identification","volume":"357","author":"H Jin","year":"2006","journal-title":"J Mol Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"14429","DOI":"10.1074\/jbc.M212747200","article-title":"A eukaryotic type serine\/threonine kinase and phosphatase in Streptococcus agalactiae reversibly phosphorylate an inorganic pyrophosphatase and affect growth, cell segregation, and virulence","volume":"278","author":"L Rajagopal","year":"2003","journal-title":"J Biol Chem"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2434","DOI":"10.1128\/IAI.72.4.2434-2437.2004","article-title":"Protein serine\/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae","volume":"72","author":"J Echenique","year":"2004","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1128\/JB.188.4.1628-1632.2006","article-title":"A eukaryotic-type serine\/threonine protein kinase is required for biofilm formation, genetic competence, and acid resistance in Streptococcus mutans","volume":"188","author":"H Hussain","year":"2006","journal-title":"J Bacteriol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S0966-842X(00)01734-0","article-title":"The eukaryotic-like Ser\/Thr protein kinases of Mycobacterium tuberculosis","volume":"8","author":"Y Av-Gay","year":"2000","journal-title":"Trends Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.1073\/pnas.0608742104","article-title":"A eukaryotic-type Ser\/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistence","volume":"104","author":"CJ Kristich","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1002\/pmic.201100259","article-title":"Analysis of the serine\/threonine\/tyrosine phosphoproteome of the pathogenic bacterium Listeria monocytogenes reveals phosphorylated proteins related to virulence","volume":"11","author":"SK Misra","year":"2011","journal-title":"Proteomics"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1046\/j.1365-2958.2002.03178.x","article-title":"Characterization of a membrane-linked Ser\/Thr protein kinase in Bacillus subtilis, implicated in developmental processes","volume":"46","author":"E Madec","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1128\/IAI.01499-08","article-title":"Modulation of cell wall structure and antimicrobial susceptibility by a Staphylococcus aureus eukaryote-like serine\/threonine kinase and phosphatase","volume":"77","author":"AM Beltramini","year":"2009","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1111\/j.1365-2958.2005.04620.x","article-title":"Regulation of purine biosynthesis by a eukaryotic-type kinase in Streptococcus agalactiae","volume":"56","author":"L Rajagopal","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.1128\/JB.01616-06","article-title":"Eukaryotic-type serine\/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae","volume":"189","author":"L Saskova","year":"2007","journal-title":"J Bacteriol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"747","DOI":"10.2217\/fmb.11.62","article-title":"Aspects of eukaryotic-like signaling in Gram-positive cocci: a focus on virulence","volume":"6","author":"K Burnside","year":"2011","journal-title":"Future Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4056","DOI":"10.1128\/JB.00117-09","article-title":"Transcriptome and functional analysis of the eukaryotic-type serine\/threonine kinase PknB in Staphylococcus aureus","volume":"191","author":"S Donat","year":"2009","journal-title":"J Bacteriol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1128\/IAI.01167-09","article-title":"The Streptococcus mutans serine\/threonine kinase, PknB, regulates competence development, bacteriocin production, and cell wall metabolism","volume":"78","author":"LD Banu","year":"2010","journal-title":"Infect Immun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1099\/mic.0.038133-0","article-title":"Functional characterization of the Mycobacterium tuberculosis serine\/threonine kinase PknJ","volume":"156","author":"J Jang","year":"2010","journal-title":"Microbiology"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1574-6968.2009.01570.x","article-title":"The serine\/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection","volume":"295","author":"R Gopalaswamy","year":"2009","journal-title":"FEMS Microbiol Lett"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.micpath.2006.02.001","article-title":"The Ser\/Thr kinase activity of the Yersinia protein kinase A (YpkA) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton","volume":"40","author":"DJ Wiley","year":"2006","journal-title":"Microb Pathog"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"4070","DOI":"10.1128\/JB.01813-08","article-title":"Characterization of a serine\/threonine kinase involved in virulence of Staphylococcus aureus","volume":"191","author":"M Debarbouille","year":"2009","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0378-1135(00)00250-9","article-title":"The pathogenesis of the meningitis caused by Streptococcus suis: the unresolved questions","volume":"76","author":"M Gottschalk","year":"2000","journal-title":"Vet Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.vetmic.2005.01.003","article-title":"Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti","volume":"107","author":"JE Hill","year":"2005","journal-title":"Vet Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"5322","DOI":"10.1128\/IAI.72.9.5322-5330.2004","article-title":"Encapsulated Streptococcus suis inhibits activation of signaling pathways involved in phagocytosis","volume":"72","author":"M Segura","year":"2004","journal-title":"Infect Immun"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.micpath.2006.04.001","article-title":"Phagocytosis and killing of Streptococcus suis by porcine neutrophils","volume":"41","author":"G Chabot-Roy","year":"2006","journal-title":"Microb Pathog"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"6154","DOI":"10.1128\/IAI.00359-06","article-title":"Identification of a novel virulence determinant with serum opacification activity in Streptococcus suis","volume":"74","author":"CG Baums","year":"2006","journal-title":"Infect Immun"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1128\/IAI.62.5.1742-1748.1994","article-title":"Identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of Streptococcus suis","volume":"62","author":"AA Jacobs","year":"1994","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1128\/IAI.70.3.1319-1325.2002","article-title":"Contribution of fibronectin-binding protein to pathogenesis of Streptococcus suis serotype 2","volume":"70","author":"A de Greeff","year":"2002","journal-title":"Infect Immun"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.micpath.2009.09.001","article-title":"Identification and characterization of inosine 5-monophosphate dehydrogenase in Streptococcus suis type 2","volume":"47","author":"XH Zhang","year":"2009","journal-title":"Microb Pathog"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1128\/JB.06231-11","article-title":"Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis","volume":"194","author":"CX Ju","year":"2012","journal-title":"J Bacteriol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e2080","DOI":"10.1371\/journal.pone.0002080","article-title":"SalK\/SalR, a two-component signal transduction system, is essential for full virulence of highly invasive Streptococcus suis serotype 2","volume":"3","author":"M Li","year":"2008","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.vetmic.2010.08.005","article-title":"Li J, Tan C, Zhou Y, Fu S, Hu L, et al. The two-component regulatory system CiaRH contributes to the virulence of Streptococcus suis 2","volume":"148","journal-title":"Vet Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1128\/JB.01309-08","article-title":"The orphan response regulator CovR: a globally negative modulator of virulence in Streptococcus suis serotype 2","volume":"191","author":"X Pan","year":"2009","journal-title":"J Bacteriol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e37825","DOI":"10.1371\/journal.pone.0037825","article-title":"Aetiologies of Central Nervous System Infection in Viet Nam: A Prospective Provincial Hospital-Based Descriptive Surveillance Study","volume":"7","author":"N Ho Dang Trung","year":"2012","journal-title":"PLoS One"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2217\/fmb.11.149","article-title":"Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent Streptococcus suis","volume":"7","author":"N Fittipaldi","year":"2012","journal-title":"Future Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1128\/IAI.68.2.637-643.2000","article-title":"Streptococcus suis serotype 2 interactions with human brain microvascular endothelial cells","volume":"68","author":"N Charland","year":"2000","journal-title":"Infect Immun"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/j.1365-2958.2006.05431.x","article-title":"Regulation of cytotoxin expression by converging eukaryotic-type and two-component signalling mechanisms in Streptococcus agalactiae","volume":"62","author":"L Rajagopal","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.micpath.2011.08.002","article-title":"The novel virulence-related gene stp of Streptococcus suis serotype 9 strain contributes to a significant reduction in mouse mortality","volume":"51","author":"H Zhu","year":"2011","journal-title":"Microb Pathog"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1006\/plas.2001.1532","article-title":"Thermosensitive suicide vectors for gene replacement in Streptococcus suis","volume":"46","author":"D Takamatsu","year":"2001","journal-title":"Plasmid"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1006\/plas.2000.1510","article-title":"Construction and characterization of Streptococcus suis-Escherichia coli shuttle cloning vectors","volume":"45","author":"D Takamatsu","year":"2001","journal-title":"Plasmid"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1128\/IAI.72.3.1441-1449.2004","article-title":"Invasion of porcine brain microvascular endothelial cells by Streptococcus suis serotype 2","volume":"72","author":"G Vanier","year":"2004","journal-title":"Infect Immun"},{"key":"ref43","first-page":"8","article-title":"Antibody response to an autogenous vaccine and serologic profile for Streptococcus suis capsular type 1\/2","volume":"66","author":"L Lapointe","year":"2002","journal-title":"Can J Vet Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1471-2180-10-42","article-title":"The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis","volume":"10","author":"L Bonifait","year":"2010","journal-title":"BMC Microbiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.4049\/jimmunol.179.3.1842","article-title":"Streptococcus suis serotype 2, an important swine and human pathogen, induces strong systemic and cerebral inflammatory responses in a mouse model of infection","volume":"179","author":"MC Dominguez-Punaro","year":"2007","journal-title":"J Immunol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"LJ Reed","year":"1938","journal-title":"American Journal of Epidemiology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1002\/pro.5560050720","article-title":"The protein phosphatase 2C (PP2C) superfamily: detection of bacterial homologues","volume":"5","author":"P Bork","year":"1996","journal-title":"Protein Sci"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1126\/science.3291115","article-title":"The protein kinase family: conserved features and deduced phylogeny of the catalytic domains","volume":"241","author":"SK Hanks","year":"1988","journal-title":"Science"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"6768","DOI":"10.1128\/JB.184.24.6768-6776.2002","article-title":"Identification and characterization of two temperature-induced surface-associated proteins of Streptococcus suis with high homologies to members of the Arginine Deiminase system of Streptococcus pyogenes","volume":"184","author":"N Winterhoff","year":"2002","journal-title":"J Bacteriol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1016\/j.vaccine.2010.01.009","article-title":"Immunoprotective activities of a Streptococcus suis pilus subunit in murine models of infection","volume":"28","author":"M Garibaldi","year":"2010","journal-title":"Vaccine"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetmic.2004.05.008","article-title":"Cloning and purification of the Streptococcus suis serotype 2 glyceraldehyde-3-phosphate dehydrogenase and its involvement as an adhesin","volume":"102","author":"J Brassard","year":"2004","journal-title":"Vet Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.vetmic.2012.02.028","article-title":"Inactivation of the sodA gene of Streptococcus suis type 2 encoding superoxide dismutase leads to reduced virulence to mice","volume":"158","author":"Y Tang","year":"2012","journal-title":"Vet Microbiol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1128\/IAI.05360-11","article-title":"Serine\/Threonine Protein Kinase Stk Is Required for Virulence, Stress Response, and Penicillin Tolerance in Streptococcus pyogenes","volume":"79","author":"J Bugrysheva","year":"2011","journal-title":"Infect Immun"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"4943","DOI":"10.1128\/JB.00196-09","article-title":"The StkP\/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization","volume":"191","author":"M Osaki","year":"2009","journal-title":"J Bacteriol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1099\/00221287-146-8-1913","article-title":"Interactions between Streptococcus suis serotype 2 and different epithelial cell lines","volume":"146 (Pt 8)","author":"M Lalonde","year":"2000","journal-title":"Microbiology"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/S0968-0004(02)02164-3","article-title":"The PASTA domain: a beta-lactam-binding domain","volume":"27","author":"C Yeats","year":"2002","journal-title":"Trends Biochem Sci"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.micpath.2010.10.001","article-title":"Trigger factor of Streptococcus suis is involved in stress tolerance and virulence","volume":"51","author":"T Wu","year":"2011","journal-title":"Microb Pathog"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1099\/mic.0.28706-0","article-title":"Defects in ex vivo and in vivo growth and sensitivity to osmotic stress of group A Streptococcus caused by interruption of response regulator gene vicR","volume":"152","author":"M Liu","year":"2006","journal-title":"Microbiology"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0091971","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T10:41:14Z","timestamp":1597574474000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0091971"}},"subtitle":[],"editor":[{"given":"Michael S.","family":"Chaussee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,3,17]]},"references-count":59,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,3,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0091971","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,17]]}}}