{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T21:04:18Z","timestamp":1783976658456,"version":"3.55.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2014,3,24]],"date-time":"2014-03-24T00:00:00Z","timestamp":1395619200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0092565","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T03:47:18Z","timestamp":1395719238000},"page":"e92565","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Inhibition of Enterovirus 71 Replication by 7-Hydroxyflavone and Diisopropyl-Flavon7-yl Phosphate"],"prefix":"10.1371","volume":"9","author":[{"given":"Jianmin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoxiang","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,3,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1093\/infdis\/129.3.304","article-title":"An apparently new enterovirus isolated from patients with disease of the central nervous system","volume":"129","author":"NJ Schmidt","year":"1974","journal-title":"J Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/S1473-3099(10)70194-8","article-title":"Virology, epidemiology, pathogenesis, and control of enterovirus 71","volume":"10","author":"T Solomon","year":"2010","journal-title":"Lancet Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3653","DOI":"10.1128\/JCM.00630-09","article-title":"Reemergence of enterovirus 71 in 2008 in taiwan: dynamics of genetic and antigenic evolution from 1998 to 2008","volume":"47","author":"SW Huang","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref4","first-page":"939","article-title":"Emerging viral infections of the central nervous system: part 1","volume":"66","author":"KL Tyler","year":"2009","journal-title":"Arch Neurol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2351","DOI":"10.1128\/JCM.00563-09","article-title":"Enterovirus 71 outbreak in the People's Republic of China in 2008","volume":"47","author":"F Yang","year":"2009","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1126\/science.1218713","article-title":"Crystal structure of human enterovirus 71","volume":"336","author":"P Plevka","year":"2012","journal-title":"Science"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/nsmb.2255","article-title":"A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71","volume":"19","author":"X Wang","year":"2012","journal-title":"Nat Struct Mol Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3710","DOI":"10.1128\/AEM.72.5.3710-3715.2006","article-title":"Use of fluorescence resonance energy transfer for rapid detection of enteroviral infection in vivo","volume":"72","author":"YC Hwang","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1023\/A:1024071514438","article-title":"Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells","volume":"25","author":"YC Hu","year":"2003","journal-title":"Biotechnol Lett"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.jmb.2011.03.007","article-title":"Crystal structure of human enterovirus 71 3C protease","volume":"408","author":"S Cui","year":"2011","journal-title":"J Mol Biol"},{"key":"ref11","first-page":"1124","article-title":"Poly (rC) binding protein 2 forms a ternary complex with the 5\u2032-terminal sequences of poliovirus RNA and the viral 3CD proteinase","volume":"3","author":"TB Parsley","year":"1997","journal-title":"RNA"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"7481","DOI":"10.1128\/JVI.66.12.7481-7489.1992","article-title":"Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase","volume":"66","author":"KS Harris","year":"1992","journal-title":"J Virol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1006\/viro.2001.1310","article-title":"The 3C protease activity of enterovirus 71 induces human neural cell apoptosis","volume":"293","author":"ML Li","year":"2002","journal-title":"Virology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e1000593","DOI":"10.1371\/journal.ppat.1000593","article-title":"Enterovirus 71 3C protease cleaves a novel target CstF-64 and inhibits cellular polyadenylation","volume":"5","author":"KF Weng","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"8811","DOI":"10.1128\/JVI.00447-11","article-title":"Cleavage of the adaptor protein TRIF by enterovirus 71 3C inhibits antiviral responses mediated by Toll-like receptor 3","volume":"85","author":"X Lei","year":"2011","journal-title":"J Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"8051","DOI":"10.1128\/JVI.02491-09","article-title":"The 3C protein of enterovirus 71 inhibits retinoid acid-inducible gene I-mediated interferon regulatory factor 3 activation and type I interferon responses","volume":"84","author":"X Lei","year":"2010","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1128\/JVI.01855-12","article-title":"Cleavage of interferon regulatory factor 7 by enterovirus 71 3C suppresses cellular responses","volume":"87","author":"X Lei","year":"2013","journal-title":"J Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/369072a0","article-title":"Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases","volume":"369","author":"M Allaire","year":"1994","journal-title":"Nature"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1002\/pro.5560040801","article-title":"The picornaviral 3C proteinases: cysteine nucleophiles in serine proteinase folds","volume":"4","author":"BA Malcolm","year":"1995","journal-title":"Protein Sci"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"11520","DOI":"10.1074\/jbc.M413254200","article-title":"Crystal structure of foot-and-mouth disease virus 3C protease. New insights into catalytic mechanism and cleavage specificity","volume":"280","author":"JR Birtley","year":"2005","journal-title":"J Biol Chem"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"7646","DOI":"10.1074\/jbc.M807947200","article-title":"Structural basis of inhibition specificities of 3C and 3C-like proteases by zinc-coordinating and peptidomimetic compounds","volume":"284","author":"CC Lee","year":"2009","journal-title":"J Biol Chem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1006\/jmbi.1997.1306","article-title":"Refined X-ray crystallographic structure of the poliovirus 3C gene product","volume":"273","author":"SC Mosimann","year":"1997","journal-title":"J Mol Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"10021","DOI":"10.1128\/JVI.05107-11","article-title":"Crystal structures of enterovirus 71 3C protease complexed with rupintrivir reveal the roles of catalytically important residues","volume":"85","author":"J Wang","year":"2011","journal-title":"J Virol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"11000","DOI":"10.1073\/pnas.96.20.11000","article-title":"Structure-assisted design of mechanism-based irreversible inhibitors of human rhinovirus 3C protease with potent antiviral activity against multiple rhinovirus serotypes","volume":"96","author":"DA Matthews","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref25","first-page":"396","article-title":"Phosphorylated modification of genistein and their interaction with lysozyme]","volume":"42","author":"X Chen","year":"2007","journal-title":"Yao xue xue bao\u200a=\u200a Acta pharmaceutica Sinica"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1021\/ac035027q","article-title":"The nature of phosphorylated chrysin-protein interactions involved in noncovalent complex formation by electrospray ionization mass spectroscopy","volume":"76","author":"X-L Chen","year":"2004","journal-title":"Anal Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"6097","DOI":"10.1016\/j.bmc.2004.09.013","article-title":"Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells","volume":"12","author":"T Zhang","year":"2004","journal-title":"Bioorg Med Chem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1086\/318454","article-title":"Neurological manifestations of enterovirus 71 infection in children during an outbreak of hand, foot, and mouth disease in Western Australia","volume":"32","author":"P McMinn","year":"2001","journal-title":"Clin Infect Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1056\/NEJM199909233411301","article-title":"An epidemic of enterovirus 71 infection in Taiwan. Taiwan Enterovirus Epidemic Working Group","volume":"341","author":"M Ho","year":"1999","journal-title":"N Engl J Med"},{"key":"ref30","first-page":"38","article-title":"[Serum types of enterovirus and clinical characteristics of 237 children with hand, foot and mouth disease in Shenzhen]","volume":"10","author":"SB Zhang","year":"2008","journal-title":"Zhongguo Dang Dai Er Ke Za Zhi"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1080\/13550280390173364","article-title":"Molecular characterization of an Enterovirus 71 causing neurological disease in Germany","volume":"9","author":"J Kehle","year":"2003","journal-title":"J Neurovirol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1093\/infdis\/169.4.905","article-title":"Enterovirus 71 infections and neurologic disease\u2014United States, 1977\u20131991","volume":"169","author":"JP Alexander Jr","year":"1994","journal-title":"J Infect Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1212\/01.WNL.0000066810.62490.FF","article-title":"Survival after pulmonary edema due to enterovirus 71 encephalitis","volume":"60","author":"MA Nolan","year":"2003","journal-title":"Neurology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0031-9422(00)00235-1","article-title":"Advances in flavonoid research since 1992","volume":"55","author":"JB Harborne","year":"2000","journal-title":"Phytochemistry"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1007\/BF02978215","article-title":"Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro","volume":"28","author":"SY Lyu","year":"2005","journal-title":"Arch Pharm Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"16785","DOI":"10.3390\/ijms131216785","article-title":"Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus","volume":"13","author":"J Johari","year":"2012","journal-title":"Int J Mol Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"7141","DOI":"10.1016\/j.bmc.2008.06.049","article-title":"Structure-activity relationship of flavonoids as influenza virus neuraminidase inhibitors and their in vitro anti-viral activities","volume":"16","author":"AL Liu","year":"2008","journal-title":"Bioorg Med Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1186\/1743-422X-8-560","article-title":"Antiviral activity of four types of bioflavonoid against dengue virus type-2","volume":"8","author":"K Zandi","year":"2011","journal-title":"Virol J"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"e30259","DOI":"10.1371\/journal.pone.0030259","article-title":"Anti-Japanese-encephalitis-viral effects of kaempferol and daidzin and their RNA-binding characteristics","volume":"7","author":"T Zhang","year":"2012","journal-title":"PLoS One"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"10319","DOI":"10.1128\/JVI.00787-11","article-title":"Enterovirus 71 and coxsackievirus A16 3C proteases: binding to rupintrivir and their substrates and anti-hand, foot, and mouth disease virus drug design","volume":"85","author":"G Lu","year":"2011","journal-title":"J Virol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"7388","DOI":"10.1016\/j.bmc.2008.06.015","article-title":"Design, synthesis, and evaluation of 3C protease inhibitors as anti-enterovirus 71 agents","volume":"16","author":"CJ Kuo","year":"2008","journal-title":"Bioorg Med Chem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cbi.2012.04.004","article-title":"Kinetics and molecular docking studies of kaempferol and its prenylated derivatives as aldose reductase inhibitors","volume":"197","author":"HA Jung","year":"2012","journal-title":"Chem Biol Interact"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility","volume":"30","author":"GM Morris","year":"2009","journal-title":"J Comput Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"LJ Reed","year":"1938","journal-title":"American Journal of Epidemiology"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0300-483X(97)00151-0","article-title":"The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines","volume":"124","author":"G Malich","year":"1997","journal-title":"Toxicology"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e38035","DOI":"10.1371\/journal.pone.0038035","article-title":"COPI is required for enterovirus 71 replication","volume":"7","author":"J Wang","year":"2012","journal-title":"PLoS One"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0092565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T19:19:11Z","timestamp":1597605551000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0092565"}},"subtitle":[],"editor":[{"given":"Eric","family":"Jan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,3,24]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,3,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0092565","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,24]]}}}