{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T05:39:59Z","timestamp":1786858799366,"version":"build-2736575974"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T00:00:00Z","timestamp":1396915200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0093805","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T21:47:16Z","timestamp":1396993636000},"page":"e93805","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":89,"title":["Simultaneous Measurement of Thirteen Steroid Hormones in Women with Polycystic Ovary Syndrome and Control Women Using Liquid Chromatography-Tandem Mass Spectrometry"],"prefix":"10.1371","volume":"9","author":[{"given":"Candace C.","family":"Keefe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mildred M.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard E.","family":"Reitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrine K.","family":"Welt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,4,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1210\/jc.2006-0178","article-title":"Criteria for defining polycystic ovary syndrome as a predominantly hyperandrogenic syndrome: an Androgen Excess Society guideline","volume":"91","author":"R Azziz","year":"2006","journal-title":"J Clin Endocrinol Metab"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1046\/j.1365-2265.1997.2321049.x","article-title":"Evidence for a primary abnormality of thecal cell steroidogenesis in the polycystic ovary syndrome","volume":"47","author":"C Gilling-Smith","year":"1997","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1210\/mend.13.6.0311","article-title":"Augmented androgen roduction is a stable steroidogenic phenotype of propagated theca cells from polycystic ovaries","volume":"13","author":"VL Nelson","year":"1999","journal-title":"Mol Endocrinol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/j.1365-2265.2005.02256.x","article-title":"Prevalence of adrenal androgen excess in patients with the polycystic ovary syndrome (PCOS)","volume":"62","author":"A Kumar","year":"2005","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"4361","DOI":"10.1210\/jc.2006-1191","article-title":"Defining constant versus variable phenotypic features of women with polycystic ovary syndrome using different ethnic groups and populations","volume":"91","author":"CK Welt","year":"2006","journal-title":"J Clin Endocrinol Metab"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/j.1365-2265.2010.03956.x","article-title":"PCOS Forum: research in polycystic ovary syndrome today and tomorrow","volume":"74","author":"R Pasquali","year":"2011","journal-title":"Clin Endocrinol (Oxf)"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1210\/jc.2006-1864","article-title":"Position statement: Utility, limitations, and pitfalls in measuring testosterone: an Endocrine Society position statement","volume":"92","author":"W Rosner","year":"2007","journal-title":"J Clin Endocrinol Metab"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"5305","DOI":"10.1210\/jc.2010-1123","article-title":"Total testosterone assays in women with polycystic ovary syndrome: precision and correlation with hirsutism","volume":"95","author":"RS Legro","year":"2010","journal-title":"J Clin Endocrinol Metab"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1530\/EJE-11-0482","article-title":"Assessment of androgen concentration in women: liquid chromatography-tandem mass spectrometry and extraction RIA show comparable results","volume":"165","author":"F Janse","year":"2011","journal-title":"Eur J Endocrinol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1055\/s-2007-981458","article-title":"Liquid chromatography tandem-mass spectrometry (LC-MS\/MS)\u2013technique and applications in endocrinology","volume":"115","author":"M Vogeser","year":"2007","journal-title":"Exp Clin Endocrinol Diabetes"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/2193-1801-2-5","article-title":"Deficiencies in immunoassay methods used to monitor serum Estradiol levels during aromatase inhibitor treatment in postmenopausal breast cancer patients","volume":"2","author":"J Jaque","year":"2013","journal-title":"SpringerPlus"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1016\/j.fertnstert.2009.12.081","article-title":"Steroid profiles in ovarian follicular fluid in women with and without polycystic ovary syndrome, analyzed by liquid chromatography-tandem mass spectrometry","volume":"94","author":"T Naessen","year":"2010","journal-title":"Fertil Steril"},{"key":"ref13","first-page":"2248","article-title":"Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome","volume":"82","author":"AE Taylor","year":"1997","journal-title":"J Clin Endocrinol Metab"},{"key":"ref14","unstructured":"Zawadzki JK, Dunaif A (1992) Diagnostic criteria for polycystic ovary syndrome: towards a rational approach. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR, editors. Polycystic Ovary Syndrome. Boston: Blackwell Scientific. 377\u2013384."},{"key":"ref15","first-page":"2163","article-title":"Inhibin A and inhibin B responses to gonadotropin withdrawal depends on stage of follicle development","volume":"84","author":"CK Welt","year":"1999","journal-title":"J Clin Endocrinol Metab"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1136\/jcp.36.10.1193","article-title":"Serum immunoglobulin concentrations in preschool children measured by laser nephelometry: reference ranges for IgG, IgA, IgM","volume":"36","author":"D Isaacs","year":"1983","journal-title":"J Clin Pathol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"744","DOI":"10.3109\/09513590.2013.798280","article-title":"Preovulatory progesterone rise during ovarian stimulation for IVF","volume":"29","author":"M Kasum","year":"2013","journal-title":"Gynecol Endocrinol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/j.fertnstert.2013.05.031","article-title":"Early initiation of gonadotropin-releasing hormone antagonist treatment results in a more stable endocrine milieu during the mid- and late-follicular phases: a randomized controlled trial comparing gonadotropin-releasing hormone antagonist initiation on cycle day 2 or 6","volume":"100","author":"O Hamdine","year":"2013","journal-title":"Fertil Steril"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1056\/NEJM198903023200904","article-title":"Pituitary-ovarian responses to nafarelin testing in the polycystic ovary syndrome","volume":"320","author":"RB Barnes","year":"1989","journal-title":"N Engl J Med"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1210\/jc.2010-2200","article-title":"Clinical evidence for predominance of delta-5 steroid production in women with polycystic ovary syndrome","volume":"96","author":"MA Rosencrantz","year":"2011","journal-title":"J Clin Endocrinol Metab"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"S77","DOI":"10.1016\/S0015-0282(02)02986-2","article-title":"Estrogen replacement therapy: effects on the endogenous androgen milieu","volume":"77","author":"JA Simon","year":"2002","journal-title":"Fertil Steril"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1210\/jcem-33-2-199","article-title":"Combined ovarian and adrenal vein catheterization to determine the site(s) of androgen overproduction in hirsute women","volume":"33","author":"MA Kirschner","year":"1971","journal-title":"J Clin Endocrinol Metab"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1210\/jcem-63-5-1204","article-title":"Determination of the source(s) of androgen overproduction in hirsutism associated with polycystic ovary syndrome by simultaneous adrenal and ovarian venous catheterization. Comparison with the dexamethasone suppression test","volume":"63","author":"BL Wajchenberg","year":"1986","journal-title":"J Clin Endocrinol Metab"},{"key":"ref24","first-page":"1158","article-title":"Hypersecretion of androstenedione by isolated thecal cells from polycystic ovaries","volume":"79","author":"C Gilling-Smith","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"5925","DOI":"10.1210\/jcem.86.12.8088","article-title":"The biochemical basis for increased testosterone production in theca cells propagated from patients with polycystic ovary syndrome","volume":"86","author":"VL Nelson","year":"2001","journal-title":"J Clin Endocrinol Metab"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3558","DOI":"10.1210\/jc.2009-0837","article-title":"Increased 5 alpha-reductase activity and adrenocortical drive in women with polycystic ovary syndrome","volume":"94","author":"DA Vassiliadi","year":"2009","journal-title":"J Clin Endocrinol Metab"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1056\/NEJM199402173300703","article-title":"Hyperandrogenism in polycystic ovary syndrome. Evidence of dysregulation of 11 beta-hydroxysteroid dehydrogenase","volume":"330","author":"A Rodin","year":"1994","journal-title":"N Engl J Med"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.steroids.2010.11.005","article-title":"Serum steroid profiling by isotopic dilution-liquid chromatography-mass spectrometry: comparison with current immunoassays and reference intervals in healthy adults","volume":"76","author":"F Fanelli","year":"2011","journal-title":"Steroids"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0093805","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T08:02:37Z","timestamp":1540281757000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0093805"}},"subtitle":[],"editor":[{"given":"Elizabeth","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,4,8]]},"references-count":28,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0093805","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,8]]}}}