{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:55:52Z","timestamp":1792670152596,"version":"4.5.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T00:00:00Z","timestamp":1397779200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0094955","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T23:55:53Z","timestamp":1397865353000},"page":"e94955","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Immobilised Histidine Tagged \u03b22-Adrenoceptor Oriented by a Diazonium Salt Reaction and Its Application in Exploring Drug-Protein Interaction Using Ephedrine and Pseudoephedrine as Probes"],"prefix":"10.1371","volume":"9","author":[{"given":"Qian","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liujiao","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,4,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"s30","DOI":"10.1016\/j.jbiotec.2010.08.088","article-title":"Characterization and modulation of molecular recognition processes: Protein\/Protein interaction in drug discovery","volume":"150","author":"C Bertucci","year":"2010","journal-title":"J Biotechnol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.patbio.2008.10.004","article-title":"Interaction networks: From protein functions to drug discovery","volume":"57","author":"E Chautard","year":"2009","journal-title":"A review Pathol. Biol"},{"key":"ref3","first-page":"e103","article-title":"Metalloproteinases: key and common mediators of multiple GPCRs and candidate therapeutic targets in models of hypertensive cardiac disease","volume":"9","author":"X Wang","year":"2012","journal-title":"Drug Discovery Today"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.bbrc.2011.07.029","article-title":"Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein","volume":"411","author":"S Zencir","year":"2011","journal-title":"Biochem Biophys Res Commun"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.ygcen.2012.11.010","article-title":"Neuroendocrine-immune interaction: Regulation of inflammation via G-protein coupled receptors","volume":"188","author":"BML Verburg-van Kemenade","year":"2013","journal-title":"Gen Comp Endocr"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.bcp.2012.09.001","article-title":"Emerging opportunities for allosteric modulation of G-protein coupled receptors","volume":"85","author":"CIA Wang","year":"2013","journal-title":"Biochem Pharmacol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jpba.2012.11.018","article-title":"A mass balance approach for calculation of recovery and binding enables the use of ultrafiltration as a rapid method for measurement of plasma protein binding for even highly lipophilic compounds","volume":"75","author":"CG Wang","year":"2013","journal-title":"J Pharm Biomed Anal"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.ymeth.2012.05.008","article-title":"Mass spectrometry approaches to monitor protein-drug interactions","volume":"57","author":"N Zinn","year":"2012","journal-title":"Methods"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jsb.2007.12.004","article-title":"A new drug binding subsite on human serum albumin and drug-drug interaction studied by X-ray crystallography","volume":"162","author":"LL Zhu","year":"2008","journal-title":"J Struct Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ijpharm.2012.09.046","article-title":"NMR techniques in drug delivery: Application to zein protein complexes","volume":"429","author":"FFO Sousa","year":"2012","journal-title":"Int J Pharm"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.neuropharm.2010.07.016","article-title":"Structure-based drug discovery and protein targets in the CNS","volume":"60","author":"RE Hubbard","year":"2011","journal-title":"Neuropharmacology"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0021-9673(97)00369-5","article-title":"Comparison of five methods for the study of drug-protein binding in affinity capillary electrophoresis","volume":"777","author":"MHA Busch","year":"1997","journal-title":"J Chromatogr A"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.addr.2011.11.011","article-title":"Structural modelling and dynamics of proteins for insights into drug interactions","volume":"64","author":"T Werner","year":"2012","journal-title":"Adv. Drug. Deliver. Rev"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1016\/j.chroma.2010.02.026","article-title":"Analysis of drug\u2013protein binding by ultrafast affinity chromatography using immobilized human serum albumin","volume":"1217","author":"R Mallik","year":"2010","journal-title":"J Chromatogr A"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1016\/j.chroma.2010.10.070","article-title":"Kinetic studies of drug\u2013protein interactions by using peak profiling and high-performance affinity chromatography: Examination of multi-site interactions of drugs with human serum albumin columns","volume":"1218","author":"ZH Tong","year":"2011","journal-title":"J Chromatogr A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0165-022X(02)00032-5","article-title":"Immobilization of invertase on rice husk using polyethylenimine","volume":"52","author":"SF D'Souza","year":"2002","journal-title":"J Biochem Biophys Methods"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1021\/bm049614c","article-title":"Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part I: development and physicochemical characteristics","volume":"6","author":"SC Rizzi","year":"2005","journal-title":"Biomacromolecules"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0165-022X(03)00109-X","article-title":"Feasibility of protein A for the oriented immobilization of immunoglobulin on silicon surface for a biosensor with imaging ellipsometry. J Biochem","volume":"57","author":"ZH Wang","year":"2003","journal-title":"Biophys Methods"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.biotechadv.2011.09.003","article-title":"Immobilization strategies to develop enzymatic biosensors","volume":"30","author":"A Sassolas","year":"2012","journal-title":"Biotechnol Adv"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.chroma.2007.05.023","article-title":"Improving immunobinding using oriented immobilization of an oxidized antibody","volume":"1161","author":"JH Kang","year":"2007","journal-title":"J Chromatogr A"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0378-4347(98)00434-4","article-title":"Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function","volume":"722","author":"J Turkov\u00e1","year":"1999","journal-title":"J Chromatogr B"},{"key":"ref22","first-page":"292","article-title":"Molecular mechanisms of <italic>\u03b2<\/italic><sub>2<\/sub>-adrenergic receptor function, response, and regulation","volume":"117","author":"M Johnson","year":"2005","journal-title":"J Allergy Clin Immun"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S0006-2952(99)00152-5","article-title":"Direct Effects of Ephedrine Isomers on Human <italic>\u03b2<\/italic>-Adrenergic Receptor Subtypes","volume":"58","author":"SS Vansal","year":"1999","journal-title":"Biochem Pharmacol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.jchromb.2009.02.031","article-title":"Thermodynamic study of the interaction between terbutaline and salbutamol with an immobilized <italic>\u03b2<\/italic><sub>2<\/sub>-adrenoceptor by high-performance liquid chromatography","volume":"877","author":"XF Zhao","year":"2009","journal-title":"J Chromatogr B"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0021-9673(94)01009-4","article-title":"Characterization of the protein binding of chiral drugs by high-performance affinity chromatography interactions of R- and S-ibuprofen with human serum albumin","volume":"693","author":"DS Hage","year":"1995","journal-title":"J Chromatogr A"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1021\/bi00514a017","article-title":"Thermodynamics of protein association reactions: forces contributing to stability","volume":"20","author":"PD Ross","year":"1981","journal-title":"Biochemistry"},{"key":"ref27","first-page":"4","article-title":"Cloning and sequence analysis of beta2-adrenergic receptor gene (written in Chinese)","volume":"19","author":"MY Li","year":"2009","journal-title":"Biotechnology"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1007\/s11434-007-0510-8","article-title":"<italic>\u03b2<\/italic><sub>2<\/sub>-adrenoceptor affinity chromatography and its application in the screening of the active compounds from semen armeniacae amarum","volume":"53","author":"XH Zheng","year":"2008","journal-title":"Chin Sci Bull"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1021\/ja043278q","article-title":"Platinum-catalyzed intramolecular hydroamination of unactivated olefins with secondary alkylamines","volume":"127","author":"FB Christopher","year":"2005","journal-title":"J Am Chem Soc"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"374","DOI":"10.3724\/SP.J.1123.2010.00374","article-title":"Two-step chromatographic method for the separation and purification of recombinant porcine <italic>\u03b2<\/italic><sub>2<\/sub>- adrenoceptors","volume":"28","author":"LH XU","year":"2010","journal-title":"Chin J Chromatogr"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"E107","DOI":"10.1093\/nar\/29.21.e107","article-title":"Robust and efficient synthetic method for forming DNA microarrays","volume":"29","author":"PL Dolan","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ref32","unstructured":"Basic Local Alignment Search Tool (BLAST) website. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi\" xlink:type=\"simple\">http:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi<\/ext-link>. Accessed 2010 Jun 3"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.jpba.2012.05.004","article-title":"Using immobilized G-protein coupled receptors to screen bioactive Traditional Chinese Medicine compounds with multiple targets","volume":"70","author":"XF Zhao","year":"2012","journal-title":"J Pharmac Biomed Analysis"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1006\/abio.1993.1165","article-title":"Defined analyte-enzyme conjugates as signal generators in immunoassays","volume":"210","author":"SH Pack","year":"1993","journal-title":"Anal Biochem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1006\/abio.1995.1501","article-title":"Irreversible, oriented immobilization of antibodies to cobalt-iminodiacetate resin for use as immunoaffinity media","volume":"231","author":"JE Hale","year":"1995","journal-title":"Anal Biochem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1111\/j.1476-5381.2010.00754.x","article-title":"The selectivity of <italic>\u03b2<\/italic>-adrenoceptor agonists at human <italic>\u03b2<\/italic><sub>1<\/sub>-, <italic>\u03b2<\/italic><sub>2<\/sub>-, <italic>\u03b2<\/italic><sub>3<\/sub>-adrenoceptors","volume":"160","author":"JG Baker","year":"2010","journal-title":"Br J Pharmacol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S0006-2952(99)00152-5","article-title":"Direct effects of ephedrine isomers on human beta-adrenergic receptor subtypes","volume":"58","author":"SS Vansal","year":"1999","journal-title":"Biochem Pharmacol"},{"key":"ref38","unstructured":"Zhang WS, Li AL (1999) Medicinal Chemistry. Beijing: Higher Education Press. 279 p."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1111\/j.1440-1681.1985.tb00891.x","article-title":"The interaction of ephedrine with <italic>\u03b2<\/italic>-adrenoceptors in tracheal, cardiac and skeletal muscles","volume":"12","author":"B Waldeck","year":"1985","journal-title":"Clin Exp Pharmacol P"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"4625","DOI":"10.1016\/S0021-9258(18)53442-6","article-title":"A mutation-induced activated state of the <italic>\u03b2<\/italic><sub>2<\/sub>-adrenergic receptor","volume":"268","author":"P Samama","year":"1993","journal-title":"J Biol Chem"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"5109a","DOI":"10.1074\/jbc.273.9.5109","article-title":"Different effects of Gs\u03b1 splice variants on <italic>\u03b2<\/italic><sub>2<\/sub>-sdrenoreceptor-mediated signalling","volume":"273","author":"R Seifert","year":"1998","journal-title":"J Biol Chem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"13572","DOI":"10.1016\/S0021-9258(18)80035-7","article-title":"Identification of two serine residues involved in agonist activation of the <italic>\u03b2<\/italic>-adrenergic receptor","volume":"264","author":"CD Strader","year":"1989","journal-title":"J Biol Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1096\/fasebj.3.7.2541037","article-title":"Structural basis of <italic>\u03b2<\/italic>-adrenergic receptor function","volume":"3","author":"CD Strader","year":"1989","journal-title":"FASEB J"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0094955","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T00:58:55Z","timestamp":1716685135000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0094955"}},"subtitle":[],"editor":[{"given":"Eugene A.","family":"Permyakov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,4,18]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0094955","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,18]]}}}