{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T06:44:57Z","timestamp":1783665897592,"version":"3.55.0"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:00:00Z","timestamp":1398038400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0095635","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T20:49:37Z","timestamp":1398113377000},"page":"e95635","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":65,"title":["Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses"],"prefix":"10.1371","volume":"9","author":[{"given":"Zheng","family":"Pang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aqian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanfu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mifang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dexin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,4,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Bray M, Johnson KM (2009) Viral Hemorrhagic Fevers: a Comparative Appraisal. Clinical Virology, Third Edition.","DOI":"10.1128\/9781555815981.ch9"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S110","DOI":"10.1038\/nm1142","article-title":"Exotic emerging viral diseases: progress and challenges","volume":"10","author":"TW Geisbert","year":"2004","journal-title":"Nat Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/scientificamerican1095-56","article-title":"Emerging viruses","volume":"273","author":"B Le Guenno","year":"1995","journal-title":"Sci Am"},{"key":"ref4","first-page":"296","article-title":"[The Alkhurma virus (family Flaviviridae, genus Flavivirus): an emerging pathogen responsible for hemorrhage fever in the Middle East]","volume":"63","author":"RN Charrel","year":"2003","journal-title":"Med Trop (Mars)"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.coi.2005.05.001","article-title":"Pathogenesis of viral hemorrhagic fever","volume":"17","author":"M Bray","year":"2005","journal-title":"Curr Opin Immunol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"e1000047","DOI":"10.1371\/journal.ppat.1000047","article-title":"Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia","volume":"4","author":"S Delgado","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e1000455","DOI":"10.1371\/journal.ppat.1000455","article-title":"Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa","volume":"5","author":"T Briese","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1056\/NEJMoa1203378","article-title":"A new phlebovirus associated with severe febrile illness in Missouri","volume":"367","author":"LK McMullan","year":"2012","journal-title":"N Engl J Med"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e1002924","DOI":"10.1371\/journal.ppat.1002924","article-title":"A novel rhabdovirus associated with acute hemorrhagic fever in central Africa","volume":"8","author":"G Grard","year":"2012","journal-title":"PLoS Pathog"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4269\/ajtmh.1997.57.519","article-title":"An outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates, 1994\u20131995","volume":"57","author":"AS Khan","year":"1997","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","first-page":"73","article-title":"Outbreak of Ebola hemorrhagic fever Uganda, August 2000-January 2001","volume":"50","year":"2001","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0166-3542(02)00201-2","article-title":"Molecular diagnostics of viral hemorrhagic fevers","volume":"57","author":"C Drosten","year":"2003","journal-title":"Antiviral Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1177\/0897190012456366","article-title":"Bioterrorism: pathogens as weapons","volume":"25","author":"PD Anderson","year":"2012","journal-title":"Journal of Pharmacy Practice"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1093\/nar\/30.6.1292","article-title":"Real-time PCR in virology","volume":"30","author":"IM Mackay","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.19.1.165-256.2006","article-title":"Real-time PCR in clinical microbiology: applications for routine laboratory testing","volume":"19","author":"MJ Espy","year":"2006","journal-title":"Clin Microbiol Rev"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1128\/JCM.40.7.2323-2330.2002","article-title":"Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR","volume":"40","author":"C Drosten","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jcv.2003.09.004","article-title":"Rapid detection protocol for filoviruses","volume":"30","author":"M Weidmann","year":"2004","journal-title":"J Clin Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jviromet.2004.10.004","article-title":"Identification of Dobrava, Hantaan, Seoul, and Puumala viruses by one-step real-time RT-PCR","volume":"124","author":"M Aitichou","year":"2005","journal-title":"J Virol Methods"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1089\/vbz.2007.0206","article-title":"A real-time RT-PCR method for the universal detection and identification of flaviviruses","volume":"7","author":"G Moureau","year":"2007","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"954","DOI":"10.4269\/ajtmh.2010.09-0636","article-title":"Comprehensive panel of real-time TaqMan polymerase chain reaction assays for detection and absolute quantification of filoviruses, arenaviruses, and New World hantaviruses","volume":"82","author":"AR Trombley","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"ref21","first-page":"E11","article-title":"Bichat guidelines for the clinical management of haemorrhagic fever viruses and bioterrorism-related haemorrhagic fever viruses","volume":"9","author":"P Bossi","year":"2004","journal-title":"Euro Surveill"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0166-3542(04)00163-9","article-title":"Crimean-Congo hemorrhagic fever","volume":"64","author":"CA Whitehouse","year":"2004","journal-title":"Antiviral Res"},{"key":"ref23","first-page":"95","article-title":"Hall TA","volume":"1999","journal-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0095635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T08:46:08Z","timestamp":1689151568000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0095635"}},"subtitle":[],"editor":[{"given":"Stefan","family":"D\u00fcbel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,4,21]]},"references-count":23,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0095635","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,21]]}}}