{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T22:41:01Z","timestamp":1789425661211,"version":"build-2803163510"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2014,5,5]],"date-time":"2014-05-05T00:00:00Z","timestamp":1399248000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0096266","type":"journal-article","created":{"date-parts":[[2014,5,5]],"date-time":"2014-05-05T20:50:13Z","timestamp":1399323013000},"page":"e96266","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Nutritional and Metabolic Requirements for the Infection of HeLa Cells by Salmonella enterica Serovar Typhimurium"],"prefix":"10.1371","volume":"9","author":[{"given":"Steven D.","family":"Bowden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda C.","family":"Hopper-Chidlaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Rice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinoy K.","family":"Ramachandran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,5,5]]},"reference":[{"key":"ref1","first-page":"346","article-title":"The global burden of typhoid fever","volume":"82","author":"JA Crump","year":"2004","journal-title":"Bull World Health Organ"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/S1286-4579(01)01491-5","article-title":"<italic>Salmonella<\/italic> intracellular proliferation: where, when and how","volume":"3","author":"F Garcia-del Portillo","year":"2001","journal-title":"Microbes Infect"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1111\/j.1462-5822.2006.00706.x","article-title":"Manipulating cellular transport and immune responses: dynamic interactions between intracellular <italic>Salmonella enterica<\/italic> and its host cells","volume":"8","author":"GL Abrahams","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/nrmicro1788","article-title":"<italic>Salmonellae<\/italic> interplay with host cells","volume":"6","author":"A Haraga","year":"2008","journal-title":"Nat Rev Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"R277","DOI":"10.1016\/S0960-9822(99)80178-X","article-title":"Bacterial invasion: Force feeding by <italic>Salmonella<\/italic>","volume":"9","author":"JH Brumell","year":"1999","journal-title":"Curr Biol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1099\/mic.0.2006\/004747-0","article-title":"Identification of novel genes in genomic islands that contribute to <italic>Salmonella typhimurium<\/italic> replication in macrophages","volume":"153","author":"J Klumpp","year":"2007","journal-title":"Microbiology"},{"key":"ref7","unstructured":"Jones BD (2005) <italic>Salmonella<\/italic> invasion gene regulation: a story of environmental awareness. J Microbiol 43 Spec No: 110\u2013117."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"5096","DOI":"10.1128\/JB.185.17.5096-5108.2003","article-title":"RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in <italic>Salmonella enterica<\/italic> serovar Typhimurium","volume":"185","author":"CD Ellermeier","year":"2003","journal-title":"J Bacteriol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"30112","DOI":"10.1074\/jbc.M605616200","article-title":"The bacterial signal molecule, ppGpp, mediates the environmental regulation of both the invasion and intracellular virulence gene programs of <italic>Salmonella<\/italic>","volume":"281","author":"A Thompson","year":"2006","journal-title":"J Biol Chem"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.mib.2005.12.010","article-title":"<italic>Salmonella<\/italic> transcriptomics: relating regulons, stimulons and regulatory networks to the process of infection","volume":"9","author":"A Thompson","year":"2006","journal-title":"Curr Opin Microbiol"},{"key":"ref11","unstructured":"Altier C (2005) Genetic and environmental control of <italic>Salmonella<\/italic> invasion. J Microbiol 43 Spec No: 85\u201392."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3117","DOI":"10.1128\/IAI.00093-09","article-title":"Glucose and glycolysis are required for the successful infection of macrophages and mice by <italic>Salmonella enterica<\/italic> serovar Typhimurium","volume":"77","author":"SD Bowden","year":"2009","journal-title":"Infect Immun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1128\/IAI.74.2.1130-1140.2006","article-title":"Role of gluconeogenesis and the tricarboxylic acid cycle in the virulence of <italic>Salmonella enterica<\/italic> serovar Typhimurium in BALB\/c mice","volume":"74","author":"MT Yimga","year":"2006","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e13871","DOI":"10.1371\/journal.pone.0013871","article-title":"An incomplete TCA cycle increases survival of <italic>Salmonella<\/italic> Typhimurium during infection of resting and activated murine macrophages","volume":"5","author":"SD Bowden","year":"2010","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1128\/IAI.01275-08","article-title":"<italic>Salmonella<\/italic>-containing vacuoles display centrifugal movement associated with cell-to-cell transfer in epithelial cells","volume":"77","author":"J Szeto","year":"2009","journal-title":"Infect Immun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1111\/j.1462-5822.2007.01099.x","article-title":"During infection of epithelial cells <italic>Salmonella enterica<\/italic> serovar Typhimurium undergoes a time-dependent transcriptional adaptation that results in simultaneous expression of three type 3 secretion systems","volume":"10","author":"I Hautefort","year":"2008","journal-title":"Cell Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in <italic>Escherichia coli<\/italic> K-12 using PCR products","volume":"97","author":"KA Datsenko","year":"2000","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0042-6822(67)90164-X","article-title":"A phage P22 gene controlling integration of prophage","volume":"31","author":"HO Smith","year":"1967","journal-title":"Virology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1046\/j.1462-5822.2000.00051.x","article-title":"<italic>Salmonella typhimurium<\/italic> mutants that downregulate phagocyte nitric oxide production","volume":"2","author":"S Eriksson","year":"2000","journal-title":"Cell Microbiol"},{"key":"ref20","unstructured":"Fraenkel DG (1996) Glycolysis. In: Neidhardt FC, Curtiss R, Ingraham JL, Lin ECC, Low KB, et al., editors. <italic>Escherichia coli<\/italic> and <italic>Salmonella<\/italic> Typhimurium:Cellular and Molecular Biology.Washington, D. C.: ASM Press. pp. 189\u2013198."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1128\/MMBR.57.4.862-952.1993","article-title":"Functions of the gene products of <italic>Escherichia coli<\/italic>","volume":"57","author":"M Riley","year":"1993","journal-title":"Microbiol Rev"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/0022-2836(89)90246-5","article-title":"Crystal structure of unliganded phosphofructokinase from <italic>Escherichia coli<\/italic>","volume":"207","author":"WR Rypniewski","year":"1989","journal-title":"J Mol Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1107\/S1744309106032246","article-title":"Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from <italic>Escherichia coli<\/italic>, a member of the ribokinase family","volume":"62","author":"R Cabrera","year":"2006","journal-title":"Acta Crystallogr Sect F Struct Biol Cryst Commun"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0304-4165(75)90232-9","article-title":"Regulation of the amount and of the activity of phosphofructokinases and pyruvate kinases in <italic>Escherichia coli<\/italic>","volume":"381","author":"D Kotlarz","year":"1975","journal-title":"Biochim Biophys Acta"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.micpath.2009.01.008","article-title":"Redundancy in the requirement for the glycolytic enzymes phosphofructokinase (Pfk) 1 and 2 in the <italic>in vivo<\/italic> fitness of <italic>Salmonella enterica<\/italic> serovar Typhimurium","volume":"46","author":"GK Paterson","year":"2009","journal-title":"Microb Pathog"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/MMBR.57.3.543-594.1993","article-title":"Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria","volume":"57","author":"PW Postma","year":"1993","journal-title":"Microbiol Rev"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.1365-2958.2003.03313.x","article-title":"Unravelling the biology of macrophage infection by gene expression profiling of intracellular <italic>Salmonella enterica<\/italic>","volume":"47","author":"S Eriksson","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"7078","DOI":"10.1016\/S0021-9258(19)41041-7","article-title":"Coordinate regulation of adenylate cyclase and carbohydrate permeases by the phosphoenolpyruvate:sugar phosphotransferase system in <italic>Salmonella typhimurium<\/italic>","volume":"250","author":"MH Saier Jr","year":"1975","journal-title":"J Biol Chem"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"14543","DOI":"10.1016\/S0021-9258(19)45412-4","article-title":"Sugar transport by the bacterial phosphotransferase system. The glucose receptors of the <italic>Salmonella typhimurium<\/italic> phosphotransferase system","volume":"257","author":"JB Stock","year":"1982","journal-title":"J Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"8585","DOI":"10.1016\/S0021-9258(18)43538-7","article-title":"Substrate specificity and kinetic characterization of sugar uptake and phosphorylation, catalyzed by the mannose enzyme II of the phosphotransferase system in <italic>Salmonella typhimurium<\/italic>","volume":"255","author":"AW Rephaeli","year":"1980","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1042\/bj1620309","article-title":"Transport of galactose, glucose and their molecular analogues by <italic>Escherichia coli<\/italic> K12","volume":"162","author":"PJ Henderson","year":"1977","journal-title":"Biochem J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1128\/JB.129.2.630-639.1977","article-title":"Galactose transport in <italic>Salmonella typhimurium<\/italic>","volume":"129","author":"PW Postma","year":"1977","journal-title":"J Bacteriol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/0923-2508(93)90002-J","article-title":"The importance of the binding-protein-dependent Mgl system to the transport of glucose in <italic>Escherichia coli<\/italic> growing on low sugar concentrations","volume":"144","author":"A Death","year":"1993","journal-title":"Res Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1271\/bbb.59.256","article-title":"Decreasing accumulation of acetate in a rich medium by <italic>Escherichia coli<\/italic> on introduction of genes on a multicopy plasmid","volume":"59","author":"K Hosono","year":"1995","journal-title":"Biosci Biotechnol Biochem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1128\/jb.179.4.1298-1306.1997","article-title":"Molecular characterization of glucokinase from <italic>Escherichia coli<\/italic> K-12","volume":"179","author":"D Meyer","year":"1997","journal-title":"J Bacteriol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e38732","DOI":"10.1371\/journal.pone.0038732","article-title":"The bimodal lifestyle of intracellular <italic>Salmonella<\/italic> in epithelial cells: replication in the cytosol obscures defects in vacuolar replication","volume":"7","author":"P Malik-Kale","year":"2012","journal-title":"PLoS One"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e84681","DOI":"10.1371\/journal.pone.0084681","article-title":"Quantitative assessment of cytosolic <italic>Salmonella<\/italic> in epithelial cells","volume":"9","author":"LA Knodler","year":"2014","journal-title":"PLoS One"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/EC.1.5.657-662.2002","article-title":"Life and death in a macrophage: role of the glyoxylate cycle in virulence","volume":"1","author":"MC Lorenz","year":"2002","journal-title":"Eukaryot Cell"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/35021074","article-title":"Persistence of <italic>Mycobacterium tuberculosis<\/italic> in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase","volume":"406","author":"JD McKinney","year":"2000","journal-title":"Nature"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1128\/IAI.73.4.2547-2549.2005","article-title":"Isocitrate lyase (AceA) is required for <italic>Salmonella<\/italic> persistence but not for acute lethal infection in mice","volume":"73","author":"FC Fang","year":"2005","journal-title":"Infect Immun"},{"key":"ref41","unstructured":"Cronan JE Jr, LaPorte D (1996) Tricarboxylic acid cycle and glyoxylate bypass. In: Neidhardt FC, Curtiss R, Ingraham JL, Lin ECC, Low KB, et al., editors. <italic>Escherichia coli<\/italic> and <italic>Salmonella<\/italic> Typhimurium: Cellular and Molecular Biology. Washington, D. C.: ASM Press. pp. 206\u2013216."},{"key":"ref42","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1128\/jb.169.7.3029-3034.1987","article-title":"Isolation and characterization of <italic>Salmonella typhimurium<\/italic> glyoxylate shunt mutants","volume":"169","author":"RB Wilson","year":"1987","journal-title":"J Bacteriol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/BF00277536","article-title":"Characterization of phosphoenolpyruvate synthase mutants in <italic>Salmonella typhimurium<\/italic>","volume":"153","author":"JR Smyer","year":"1989","journal-title":"Arch Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1128\/JB.102.2.524-530.1970","article-title":"Mutants of <italic>Salmonella typhimurium<\/italic> lacking phosphoenolpyruvate carboxykinase and alpha-ketoglutarate dehydrogenase activities","volume":"102","author":"G Carrillo-Castaneda","year":"1970","journal-title":"J Bacteriol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/2049-1891-4-5","article-title":"Deletions in the pyruvate pathway of <italic>Salmonella<\/italic> Typhimurium alter SPI1-mediated gene expression and infectivity","volume":"4","author":"J Abernathy","year":"2013","journal-title":"J Anim Sci Biotechnol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e1003301","DOI":"10.1371\/journal.ppat.1003301","article-title":"Parallel exploitation of diverse host nutrients enhances <italic>Salmonella<\/italic> virulence","volume":"9","author":"B Steeb","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e10586","DOI":"10.1371\/journal.pone.0010586","article-title":"Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells","volume":"5","author":"A Gotz","year":"2010","journal-title":"PLoS One"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/BF02190806","article-title":"Regulation of glycerol and maltose uptake by the IIAGlc-like domain of IINag of the phosphotransferase system in <italic>Salmonella typhimurium<\/italic> LT2","volume":"248","author":"J van der Vlag","year":"1995","journal-title":"Mol Gen Genet"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1128\/MMBR.00024-06","article-title":"How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria","volume":"70","author":"J Deutscher","year":"2006","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0034-5288(18)32968-0","article-title":"Experimental <italic>Salmonella typhimurium<\/italic> infection in calves","volume":"25","author":"C Wray","year":"1978","journal-title":"Res Vet Sci"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0378-1119(91)90366-J","article-title":"Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in <italic>Escherichia coli<\/italic>","volume":"100","author":"RF Wang","year":"1991","journal-title":"Gene"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0096266","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T23:54:36Z","timestamp":1648943676000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0096266"}},"subtitle":[],"editor":[{"given":"Yousef Abu","family":"Kwaik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,5,5]]},"references-count":51,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,5,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0096266","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,5]]}}}