{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:13:05Z","timestamp":1778865185318,"version":"3.51.4"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0096548","type":"journal-article","created":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T20:52:43Z","timestamp":1398977563000},"page":"e96548","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Effect of Substrate Elasticity and Actomyosin Contractility on Different Forms of Endocytosis"],"prefix":"10.1371","volume":"9","author":[{"given":"Dimitris","family":"Missirlis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,5,1]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1242\/jcs.093005","article-title":"Mechanosensitive mechanisms in transcriptional regulation","volume":"125","author":"A Mammoto","year":"2012","journal-title":"Journal of Cell Science"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/nrm2593","article-title":"Environmental sensing through focal adhesions","volume":"10","author":"B Geiger","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1038\/nrm2597","article-title":"Mechanotransduction gone awry","volume":"10","author":"DE Jaalouk","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/nrm3112","article-title":"Balancing forces: architectural control of mechanotransduction","volume":"12","author":"CC Dufort","year":"2011","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1146\/annurev.biochem.78.081307.110540","article-title":"Mechanisms of Endocytosis","volume":"78","author":"GJ Doherty","year":"2009","journal-title":"Annu Rev Biochem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature08910","article-title":"The endocytic matrix","volume":"463","author":"G Scita","year":"2010","journal-title":"Nature"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrm3151","article-title":"Molecular mechanism and physiological functions of clathrin-mediated endocytosis","volume":"12","author":"HT Mcmahon","year":"2011","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1242\/jcs.033951","article-title":"Molecular mechanisms of clathrin-independent endocytosis","volume":"122","author":"CG Hansen","year":"2009","journal-title":"Journal of Cell Science"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1038\/nrm2748","article-title":"Endocytosis and signalling: intertwining molecular networks","volume":"10","author":"A Sorkin","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1039\/c2cs15309b","article-title":"Endocytosis at the nanoscale","volume":"41","author":"I Canton","year":"2012","journal-title":"Chem Soc Rev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1146\/annurev-biochem-060208-104626","article-title":"Virus Entry by Endocytosis","volume":"79","author":"J Mercer","year":"2010","journal-title":"Annu Rev Biochem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/nature08779","article-title":"Systems survey of endocytosis by multiparametric image analysis","volume":"464","author":"C Collinet","year":"2010","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature08282","article-title":"Population context determines cell-to-cell variability in endocytosis and virus infection","volume":"461","author":"B Snijder","year":"2009","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"F179","DOI":"10.1152\/ajprenal.2002.282.2.F179","article-title":"Modulation of membrane traffic by mechanical stimuli","volume":"282","author":"G Apodaca","year":"2002","journal-title":"Am J Physiol Renal Physiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1038\/ncb2307","article-title":"Actin dynamics counteract membrane tension during clathrin-mediated endocytosis","volume":"13","author":"S Boulant","year":"2011","journal-title":"Nat Cell Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1038\/nmat1392","article-title":"Non-viral gene delivery regulated by stiffness of cell adhesion substrates","volume":"4","author":"H-J Kong","year":"2005","journal-title":"Nat Mater"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1021\/nl400033h","article-title":"Substrate stiffness regulates cellular uptake of nanoparticles","volume":"13","author":"C Huang","year":"2013","journal-title":"Nano Lett"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.nantod.2010.10.007","article-title":"Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery","volume":"5","author":"AF Adler","year":"2010","journal-title":"Nano Today"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.cub.2009.07.069","article-title":"Cell-cycle control by physiological matrix elasticity and in vivo tissue stiffening","volume":"19","author":"EA Klein","year":"2009","journal-title":"Curr Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/j.cell.2009.10.027","article-title":"Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling","volume":"139","author":"KR Levental","year":"2009","journal-title":"Cell"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Missirlis D, Spatz JP (2013) Combined Effects of PEG Hydrogel Elasticity and Cell-Adhesive Coating on Fibroblast Adhesion and Persistent Migration. Biomacromolecules: 131205085858005. doi:<ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1021\/bm4014827\" xlink:type=\"simple\">10.1021\/bm4014827<\/ext-link>.","DOI":"10.1021\/bm4014827"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6044","DOI":"10.1529\/biophysj.107.127662","article-title":"Cell-Cell Mechanical Communication through Compliant Substrates","volume":"95","author":"CA Reinhart-King","year":"2008","journal-title":"Biophysical Journal"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Hopkins CR, Miller K, Beardmore JM (1985) Receptor-mediated endocytosis of transferrin and epidermal growth factor receptors: a comparison of constitutive and ligand-induced uptake. J Cell Sci Suppl 3: 173\u2013186.","DOI":"10.1242\/jcs.1985.Supplement_3.17"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1574-6968.2006.00545.x","article-title":"Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER","volume":"266","author":"DJF Chinnapen","year":"2007","journal-title":"FEMS Microbiology Letters"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/nrm1315","article-title":"Endocytic recycling","volume":"5","author":"FR Maxfield","year":"2004","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"3737","DOI":"10.1242\/jcs.114.20.3737","article-title":"Internalization of cholera toxin by different endocytic mechanisms","volume":"114","author":"ML Torgersen","year":"2001","journal-title":"Journal of Cell Science"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"4453","DOI":"10.1529\/biophysj.106.101386","article-title":"Fibroblast adaptation and stiffness matching to soft elastic substrates","volume":"93","author":"J Solon","year":"2007","journal-title":"Biophysical Journal"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"6933","DOI":"10.1073\/pnas.1117810109","article-title":"Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness","volume":"109","author":"L Trichet","year":"2012","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref29","first-page":"144","article-title":"Cell Movement Is Guided by the Rigidity of the Substrate","volume":"79","author":"C-M Lo","year":"2008","journal-title":"Biophysical Journal"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"5095","DOI":"10.1016\/j.bpj.2009.03.046","article-title":"Absence of Filamin A Prevents Cells from Responding to Stiffness Gradients on Gels Coated with Collagen but not Fibronectin","volume":"96","author":"FJ Byfield","year":"2009","journal-title":"Biophysical Journal"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/ncb2747","article-title":"beta1- and alphav-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments","volume":"15","author":"HB Schiller","year":"2013","journal-title":"Nat Cell Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1083\/jcb.200906012","article-title":"Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation","volume":"188","author":"AM Pasapera","year":"2010","journal-title":"The Journal of Cell Biology"},{"key":"ref33","first-page":"5974","article-title":"Matrix stiffness reverses the effect of actomyosin tension on cell proliferation","volume":"125","author":"JD Mih","year":"2013","journal-title":"Journal of Cell Science"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1016\/S0026-895X(24)26508-1","article-title":"Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases","volume":"57","author":"T Ishizaki","year":"2000","journal-title":"Mol Pharmacol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"35557","DOI":"10.1074\/jbc.M405319200","article-title":"Mechanism of blebbistatin inhibition of myosin II","volume":"279","author":"M Kov\u00e1cs","year":"2004","journal-title":"J Biol Chem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1242\/jcs.058297","article-title":"Cytoskeletal coherence requires myosin-IIA contractility","volume":"123","author":"Y Cai","year":"2010","journal-title":"Journal of Cell Science"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e35046","DOI":"10.1371\/journal.pone.0035046","article-title":"Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases","volume":"7","author":"A Dhaliwal","year":"2012","journal-title":"PLoS ONE"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"3436","DOI":"10.1016\/j.actbio.2010.03.038","article-title":"Differential uptake of DNA-poly(ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces","volume":"6","author":"A Dhaliwal","year":"2010","journal-title":"ACTA BIOMATERIALIA"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"3534","DOI":"10.1242\/jcs.006296","article-title":"Rho\/ROCK and myosin II control the polarized distribution of endocytic clathrin structures at the uropod of moving T lymphocytes","volume":"120","author":"R Samaniego","year":"2007","journal-title":"Journal of Cell Science"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1002\/cm.20220","article-title":"Assessment of myosin II, Va, VI and VIIa loss of function on endocytosis and endocytic vesicle motility in bone marrow-derived dendritic cells","volume":"64","author":"JP Holt","year":"2007","journal-title":"Cell Motil Cytoskeleton"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/bj20031253","article-title":"Size-dependent internalization of particles via the pathways of clathrin-and caveolae-mediated endocytosis","volume":"377","author":"J Rejman","year":"2004","journal-title":"Biochem J"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1002\/jbm.a.32648","article-title":"In vitro uptake of amphiphilic, hydrogel nanoparticles by J774A.1 cells","volume":"93A","author":"D Missirlis","year":"2009","journal-title":"J Biomed Mater Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2988","DOI":"10.1002\/bit.23253","article-title":"Endothelial nanoparticle binding kinetics are matrix and size dependent","volume":"108","author":"AL Doiron","year":"2011","journal-title":"Biotechnol Bioeng"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1021\/nl070363y","article-title":"Elucidating the Mechanism of Cellular Uptake and Removal of Protein-Coated Gold Nanoparticles of Different Sizes and Shapes","volume":"7","author":"BD Chithrani","year":"2007","journal-title":"Nano Lett"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1002\/smll.201101076","article-title":"Quantitative Assessment of the Comparative Nanoparticle-Uptake Efficiency of a Range of Cell Lines","volume":"7","author":"T Santos dos","year":"2011","journal-title":"Small"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1021\/nl0803848","article-title":"Cellular trafficking of quantum dot-ligand bioconjugates and their induction of changes in normal routing of unconjugated ligands","volume":"8","author":"C Tekle","year":"2008","journal-title":"Nano Lett"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S0006-3495(85)83838-8","article-title":"The effect of iron binding on the conformation of transferrin. A small angle x-ray scattering study","volume":"48","author":"F Kil\u00e1r","year":"1985","journal-title":"Biophysical Journal"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/nrc775","article-title":"HeLa cells 50 years on: the good, the bad and the ugly","volume":"2","author":"JR Masters","year":"2002","journal-title":"Nat Rev Cancer"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1038\/nrc2521","article-title":"Derailed endocytosis: an emerging feature of cancer","volume":"8","author":"Y Mosesson","year":"2008","journal-title":"Nat Rev Cancer"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"C1345","DOI":"10.1152\/ajpcell.2000.279.5.C1345","article-title":"Substrate flexibility regulates growth and apoptosis of normal but not transformed cells","volume":"279","author":"H Wang","year":"2000","journal-title":"Am J Physiol-Cell Ph"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1039\/C1SM06591B","article-title":"Extracellular matrix modulates non-viral gene transfer to mouse mesenchymal stem cells","volume":"8","author":"A Dhaliwal","year":"2012","journal-title":"Soft Matter"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nmat3387","article-title":"Extracellular-matrix tethering regulates stem-cell fate","volume":"11","author":"B Trappmann","year":"2012","journal-title":"Nat Mater"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.jbiomech.2011.11.023","article-title":"Reprogramming cardiomyocyte mechanosensing by crosstalk between integrins and hyaluronic acid receptors","volume":"45","author":"A Chopra","year":"2012","journal-title":"Journal of Biomechanics"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"4881","DOI":"10.1016\/j.biomaterials.2006.05.012","article-title":"The use of poly(ethylene glycol) hydrogels to investigate the impact of ECM chemistry and mechanics on smooth muscle cells","volume":"27","author":"SR Peyton","year":"2006","journal-title":"Biomaterials"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1091\/mbc.E11-06-0537","article-title":"Matrix rigidity regulates a switch between TGF- 1-induced apoptosis and epithelial-mesenchymal transition","volume":"23","author":"JL Leight","year":"2012","journal-title":"Mol Biol Cell"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1021\/bm025744e","article-title":"Synthesis and Physicochemical Characterization of End-Linked Poly(ethylene glycol)- co-peptide Hydrogels Formed by Michael-Type Addition","volume":"4","author":"MP Lutolf","year":"2003","journal-title":"Biomacromolecules"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/1743-422X-5-163","article-title":"Ephrin-B2 expression critically influences Nipah virus infection independent of its cytoplasmic tail","volume":"5","author":"L Thiel","year":"2008","journal-title":"Virol J"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0096548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T17:02:56Z","timestamp":1746205376000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0096548"}},"subtitle":[],"editor":[{"given":"Adam J.","family":"Engler","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,5,1]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,5,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0096548","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,1]]}}}