{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T20:45:11Z","timestamp":1789073111880,"version":"build-2803163510"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0096930","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T20:22:12Z","timestamp":1400530932000},"page":"e96930","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Simple Technique for in Field Samples Collection in the Cases of Skin Rash Illness and Subsequent PCR Detection of Orthopoxviruses and Varicella Zoster Virus"],"prefix":"10.1371","volume":"9","author":[{"given":"Catherine","family":"Dumont","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid M.","family":"Irenge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmond K.","family":"Magazani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Garin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques T.","family":"Muyembe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Bentahir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Gala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jcpa.2012.06.007","article-title":"Comparative pathology of smallpox and monkeypox in man and macaques","volume":"148","author":"JA Cann","year":"2013","journal-title":"J Comp Pathol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.tim.2008.07.009","article-title":"Vaccinia virus morphogenesis and dissemination","volume":"16","author":"KL Roberts","year":"2008","journal-title":"Trends Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0966-842X(00)01855-2","article-title":"The threat of smallpox and bioterrorism","volume":"9","author":"P Berche","year":"2001","journal-title":"Trends Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1067\/mjd.2001.109302","article-title":"An overview of poxviruses","volume":"44","author":"DG Diven","year":"2001","journal-title":"J Am Acad Dermatol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1128\/CMR.14.2.364-381.2001","article-title":"Bioterrorism: implications for the clinical microbiologist","volume":"14","author":"WF Klietmann","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.tim.2011.12.001","article-title":"Outbreaks of human monkeypox after cessation of smallpox vaccination","volume":"20","author":"MG Reynolds","year":"2012","journal-title":"Trends Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3201\/eid0802.010164","article-title":"Public health assessment of potential biological terrorism agents","volume":"8","author":"LD Rotz","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"499","DOI":"10.4269\/ajtmh.2009.80.499","article-title":"Keeping an eye on poxviruses","volume":"80","author":"M Bray","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref9","first-page":"569","article-title":"Human monkeypox","volume":"46","author":"SO Foster","year":"1972","journal-title":"Bull World Health Organ"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/infdis\/156.2.293","article-title":"Human monkeypox: clinical features of 282 patients","volume":"156","author":"Z Jezek","year":"1987","journal-title":"J Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1093\/oxfordjournals.bmb.a011720","article-title":"Re-emergence of monkeypox in Africa: a review of the past six years","volume":"54","author":"DL Heymann","year":"1998","journal-title":"Br Med Bull"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1086\/595552","article-title":"Transmission of atypical varicella-zoster virus infections involving palm and sole manifestations in an area with monkeypox endemicity","volume":"48","author":"A Macneil","year":"2009","journal-title":"Clin Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.jviromet.2008.07.018","article-title":"Dual-probe real-time PCR assay for detection of variola or other orthopoxviruses with dried reagents","volume":"153","author":"M Aitichou","year":"2008","journal-title":"J Virol Methods"},{"key":"ref14","first-page":"297","article-title":"Human monkeypox: confusion with chickenpox","volume":"45","author":"Z Jezek","year":"1988","journal-title":"Acta Trop"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"503","DOI":"10.4269\/ajtmh.2009.80.503","article-title":"Monkeypox or varicella? Lessons from a rash outbreak investigation in the Republic of the Congo","volume":"80","author":"A MacNeil","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"934","DOI":"10.3201\/eid1306.061540","article-title":"Endemic human monkeypox, Democratic Republic of Congo, 2001\u20132004","volume":"13","author":"AW Rimoin","year":"2007","journal-title":"Emerg Infect Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1086\/498155","article-title":"Reemergence of monkeypox: prevalence, diagnostics, and countermeasures","volume":"41","author":"A Nalca","year":"2005","journal-title":"Clin Infect Dis"},{"key":"ref18","unstructured":"Gala JLIL (2011) From a reach back capacity to the rapid deployment of a light mobile fieldable unit: a case for better European crisis management of natural, emerging and terrorist biological threats. Medical Biodefense Conference, Munich."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1373\/clinchem.2006.068635","article-title":"Real-time PCR to identify variola virus or other human pathogenic orthopox viruses","volume":"53","author":"N Scaramozzino","year":"2007","journal-title":"Clin Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1186\/1756-3305-5-288","article-title":"Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples","volume":"5","author":"JF Durant","year":"2012","journal-title":"Parasit Vectors"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s00253-010-2848-0","article-title":"Development and validation of a real-time quantitative PCR assay for rapid identification of Bacillus anthracis in environmental samples","volume":"88","author":"LM Irenge","year":"2010","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/S0140-6736(47)91671-1","article-title":"Survival of variola virus in dried exudate and crusts from smallpox patients","volume":"1","author":"AW Downie","year":"1947","journal-title":"Lancet"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/0035-9203(85)90333-5","article-title":"The histopathology and electron microscopy of a human monkeypox lesion","volume":"79","author":"MJ Stagles","year":"1985","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref25","first-page":"703","article-title":"The current status of human monkeypox: memorandum from a WHO meeting","volume":"62","year":"1984","journal-title":"Bull World Health Organ"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1473-3099(03)00856-9","article-title":"Human monkeypox: an emerging zoonosis","volume":"4","author":"DB Di Giulio","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e6342","DOI":"10.1371\/journal.pone.0006342","article-title":"Rapid and high-throughput pan-Orthopoxvirus detection and identification using PCR and mass spectrometry","volume":"4","author":"MW Eshoo","year":"2009","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JCM.42.2.601-609.2004","article-title":"Smallpox and pan-orthopox virus detection by real-time 3\u2032-minor groove binder TaqMan assays on the roche LightCycler and the Cepheid smart Cycler platforms","volume":"42","author":"DA Kulesh","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Y Li","year":"2010","journal-title":"J Virol Methods"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1002\/jmv.21385","article-title":"Detection of human orthopoxvirus infections and differentiation of smallpox virus with real-time PCR","volume":"81","author":"N Putkuri","year":"2009","journal-title":"J Med Virol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"140","DOI":"10.7883\/yoken.JJID.2008.140","article-title":"Diagnosis and assessment of monkeypox virus (MPXV) infection by quantitative PCR assay: differentiation of Congo Basin and West African MPXV strains","volume":"61","author":"M Saijo","year":"2008","journal-title":"Jpn J Infect Dis"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jviromet.2011.05.002","article-title":"Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay","volume":"175","author":"SN Shchelkunov","year":"2011","journal-title":"J Virol Methods"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1128\/JCM.40.8.2919-2921.2002","article-title":"Outbreaks of disease suspected of being due to human monkeypox virus infection in the Democratic Republic of Congo in 2001","volume":"40","author":"H Meyer","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1128\/JCM.02015-10","article-title":"Lyssavirus detection and typing using pyrosequencing","volume":"49","author":"P De Benedictis","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1128\/JCM.00920-06","article-title":"Zoonotic vaccinia virus infection in Brazil: clinical description and implications for health professionals","volume":"45","author":"G de Souza Trindade","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e23400","DOI":"10.1371\/journal.pone.0023400","article-title":"Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing","volume":"6","author":"YM Deng","year":"2011","journal-title":"PLoS One"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1093\/bioinformatics\/btt339","article-title":"AdvISER-PYRO: Amplicon Identification using SparsE Representation of PYROsequencing signal","volume":"29","author":"J Ambroise","year":"2013","journal-title":"Bioinformatics"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e1003756","DOI":"10.1371\/journal.ppat.1003756","article-title":"An increasing danger of zoonotic orthopoxvirus infections","volume":"9","author":"SN Shchelkunov","year":"2013","journal-title":"PLoS Pathog"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0096930","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T06:59:09Z","timestamp":1689231549000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0096930"}},"subtitle":[],"editor":[{"given":"Pierre","family":"Roques","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,5,19]]},"references-count":38,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,5,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0096930","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,19]]}}}