{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:56:13Z","timestamp":1789732573846,"version":"4.0.1"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T00:00:00Z","timestamp":1399939200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0097468","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T20:55:14Z","timestamp":1400014514000},"page":"e97468","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":28,"title":["Actions of Agonists, Fipronil and Ivermectin on the Predominant In Vivo Splice and Edit Variant (RDLbd, I\/V) of the Drosophila GABA Receptor Expressed in Xenopus laevis Oocytes"],"prefix":"10.1371","volume":"9","author":[{"given":"Kristin","family":"Lees","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Musgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siros","family":"Suwanmanee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven David","family":"Buckingham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Biggin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sattelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.tips.2009.12.005","article-title":"Binding, activation and modulation of Cys-loop receptors","volume":"31","author":"PS Miller","year":"2010","journal-title":"Trends Pharmacol Sci"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/j.1476-5381.1980.tb10937.x","article-title":"Do motor-nerve terminals have gamma-aminobutyric acid receptors?","volume":"71","author":"TG Smart","year":"1980","journal-title":"Br J Pharmacol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10158-012-0129-6","article-title":"Nematode cys-loop GABA receptors: biological function, pharmacology and sites of action for anthelmintics","volume":"12","author":"MV Accardi","year":"2012","journal-title":"Invert Neurosci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/S0166-2236(97)01127-2","article-title":"Molecular biology of insect neuronal GABA receptors","volume":"20","author":"AM Hosie","year":"1997","journal-title":"Trends Neurosci"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2806(08)60005-3","article-title":"GABA receptors of insects","volume":"22","author":"DB Sattelle","year":"1990","journal-title":"Advances in insect physiology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"5707","DOI":"10.1523\/JNEUROSCI.5663-11.2012","article-title":"Benzodiazepines modulate GABAA receptors by regulating the preactivation step after GABA binding","volume":"32","author":"MC Gielen","year":"2012","journal-title":"J Neurosci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1021\/jm970681h","article-title":"Propofol analogues. Synthesis, relationships between structure and affinity at GABAA receptor in rat brain, and differential electrophysiological profile at recombinant human GABAA receptors","volume":"41","author":"G Trapani","year":"1998","journal-title":"J Med Chem"},{"key":"ref8","first-page":"75","article-title":"Replacement of asparagine with arginine at the extracellular end of the second transmembrane (M2) region of insect GABA receptors increases sensitivity to penicillin G. Invert Neurosci","volume":"6","author":"AM Hosie","year":"2006"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1476-5381.1982.tb09294.x","article-title":"Electrophysiological effects of piperazine and diethylcarbamazine on <italic>Ascaris suum<\/italic> somatic muscle","volume":"77","author":"RJ Martin","year":"1982","journal-title":"Br J Pharmacol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/363449a0","article-title":"A point mutation in a Drosophila GABA receptor confers insecticide resistance","volume":"363","author":"RH Ffrench-Constant","year":"1993","journal-title":"Nature"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"7209","DOI":"10.1073\/pnas.88.16.7209","article-title":"Molecular cloning and transformation of cyclodiene resistance in Drosophila: an invertebrate gamma-aminobutyric acid subtype A receptor locus","volume":"88","author":"RH Ffrench-Constant","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s004380050882","article-title":"Duplication of the Rdl GABA receptor subunit gene in an insecticide-resistant aphid, <italic>Myzus persicae<\/italic>","volume":"260","author":"N Anthony","year":"1998","journal-title":"Mol Gen Genet"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1002\/ps.937","article-title":"Identification of the Rdl mutation in laboratory and field strains of the cat flea, <italic>Ctenocephalides felis<\/italic> (Siphonaptera: Pulicidae)","volume":"60","author":"C Bass","year":"2004","journal-title":"Pest Manag Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/0305-0491(95)00007-U","article-title":"DNA sequence and site of mutation of the GABA receptor of cyclodiene-resistant red flour beetle, <italic>Tribolium castaneum<\/italic>","volume":"111","author":"M Miyazaki","year":"1995","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1111\/j.1471-4159.2004.02922.x","article-title":"Resistance to fipronil in <italic>Drosophila simulans<\/italic>: influence of two point mutations in the RDL GABA receptor subunit","volume":"92","author":"G Le Goff","year":"2005","journal-title":"J Neurochem"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/PL00006504","article-title":"Multiple origins of cyclodiene insecticide resistance in <italic>Tribolium castaneum<\/italic> (Coleoptera: Tenebrionidae)","volume":"48","author":"D Andreev","year":"1999","journal-title":"J Mol Evol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1093\/jee\/83.5.1733","article-title":"Isolation of dieldrin resistance from field populations of <italic>Drosophila melanogaster<\/italic> (Diptera: Drosophilidae)","volume":"83","author":"RH Ffrench-Constant","year":"1990","journal-title":"J Econ Entomol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"4287","DOI":"10.1523\/JNEUROSCI.5251-08.2009","article-title":"Splice-variant- and stage-specific RNA editing of the Drosophila GABA receptor modulates agonist potency","volume":"29","author":"AK Jones","year":"2009","journal-title":"J Neurosci"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0306-4522(00)00483-8","article-title":"Alternative splicing of a Drosophila GABA receptor subunit gene identifies determinants of agonist potency","volume":"102","author":"AM Hosie","year":"2001","journal-title":"Neuroscience"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1126\/science.1086763","article-title":"Nervous system targets of RNA editing identified by comparative genomics","volume":"301","author":"B Hoopengardner","year":"2003","journal-title":"Science"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1097\/WNR.0b013e32830216c7","article-title":"RNA editing regulates insect gamma-aminobutyric acid receptor function and insecticide sensitivity","volume":"19","author":"Z Es-Salah","year":"2008","journal-title":"Neuroreport"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1113\/jphysiol.2003.051839","article-title":"The alpha 1 and alpha 6 subunit subtypes of the mammalian GABA(A) receptor confer distinct channel gating kinetics","volume":"561","author":"JL Fisher","year":"2004","journal-title":"J Physiol"},{"key":"ref23","unstructured":"Sali A, Fiser A, Yerkovich B, Eswar N, Marti-Renom M, <etal>et al<\/etal>.. (1994) Modeller. A program for protein structure modelling by satisfaction of spatial restraints."},{"key":"ref24","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1038\/nature10139","article-title":"Principles of activation and permeation in an anion-selective Cys-loop receptor","volume":"474","author":"RE Hibbs","year":"2011","journal-title":"Nature"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"W695","DOI":"10.1093\/nar\/gkq313","article-title":"A new bioinformatics analysis tools framework at EMBL-EBI","volume":"38","author":"M Goujon","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"MA Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0022-5193(87)80144-3","article-title":"3- and 5-isoxazolol zwitterions: an ab initio molecular orbital study relating to GABA agonism and antagonism","volume":"127","author":"T Boulanger","year":"1987","journal-title":"J Theor Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.3891\/acta.chem.scand.26-1298","article-title":"The structure of muscimol, a GABA analogue of restricted conformation","volume":"26","author":"L Brehm","year":"1972","journal-title":"Acta Chem Scand"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/S0003-9861(02)00692-6","article-title":"Thermodynamics of the reduction of NADP with 2-propanol catalyzed by an NADP-dependent alcohol dehydrogenase","volume":"410","author":"MT King","year":"2003","journal-title":"Arch Biochem Biophys"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Krogsgaard-Larsen P, Falch E (1981) GABA agonists. Development and interactions with the GABA receptor complex. Mol Cell Biochem 38 Spec No: 129\u2013146.","DOI":"10.1007\/BF00235692"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility","volume":"30","author":"GM Morris","year":"2009","journal-title":"J Comput Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"O Trott","year":"2010","journal-title":"J Comput Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.1529\/biophysj.107.127589","article-title":"Molecular dynamics simulations of GABA binding to the GABAC receptor: the role of Arg104","volume":"95","author":"C Melis","year":"2008","journal-title":"Biophys J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.jmgm.2008.12.004","article-title":"Molecular modeling of ligand-receptor interactions in GABA C receptor","volume":"27","author":"DI Osolodkin","year":"2009","journal-title":"J Mol Graph Model"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"12371","DOI":"10.1523\/JNEUROSCI.1610-11.2011","article-title":"Two amino acid residues contribute to a cation-pi binding interaction in the binding site of an insect GABA receptor","volume":"31","author":"SC Lummis","year":"2011","journal-title":"J Neurosci"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"20906","DOI":"10.1074\/jbc.M401012200","article-title":"Evidence that the TM1\u2013TM2 loop contributes to the rho1 GABA receptor pore","volume":"279","author":"N Filippova","year":"2004","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1111\/j.1476-5381.1996.tb16075.x","article-title":"Agonist pharmacology of two Drosophila GABA receptor splice variants","volume":"119","author":"AM Hosie","year":"1996","journal-title":"Br J Pharmacol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/j.1365-2583.2006.00680.x","article-title":"Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes","volume":"15","author":"Y Eguchi","year":"2006","journal-title":"Insect Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1021\/bi901698c","article-title":"Molecular characterization of agonists that bind to an insect GABA receptor","volume":"49","author":"I McGonigle","year":"2010","journal-title":"Biochemistry"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1124\/mol.105.015313","article-title":"Insect GABA receptors: splicing, editing, and targeting by antiparasitics and insecticides","volume":"68","author":"SD Buckingham","year":"2005","journal-title":"Mol Pharmacol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e52323","DOI":"10.1371\/journal.pone.0052323","article-title":"A unified model of the GABA(A) receptor comprising agonist and benzodiazepine binding sites","volume":"8","author":"R Bergmann","year":"2013","journal-title":"PLoS One"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"6548","DOI":"10.1021\/bi015920o","article-title":"Ivermectin and nodulisporic acid receptors in <italic>Drosophila melanogaster<\/italic> contain both gamma-aminobutyric acid-gated Rdl and glutamate-gated GluCl alpha chloride channel subunits","volume":"41","author":"SW Ludmerer","year":"2002","journal-title":"Biochemistry"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.pestbp.2009.10.002","article-title":"The role of GABA and glutamate receptors in susceptibility and resistance to chloride channel blocker insecticides","volume":"97","author":"X Zhao","year":"2010","journal-title":"Pesticide biochemistry and physiology"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"13949","DOI":"10.1073\/pnas.240464697","article-title":"Drug-resistant Drosophila indicate glutamate-gated chloride channels are targets for the antiparasitics nodulisporic acid and ivermectin","volume":"97","author":"NS Kane","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"40232","DOI":"10.1074\/jbc.R112.406280","article-title":"Glutamate-gated chloride channels","volume":"287","author":"AJ Wolstenholme","year":"2012","journal-title":"J Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1124\/mol.53.2.283","article-title":"Ivermectin: a positive allosteric effector of the alpha7 neuronal nicotinic acetylcholine receptor","volume":"53","author":"RM Krause","year":"1998","journal-title":"Mol Pharmacol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.bcp.2009.06.020","article-title":"Comparative pharmacology and computational modelling yield insights into allosteric modulation of human alpha7 nicotinic acetylcholine receptors","volume":"78","author":"DB Sattelle","year":"2009","journal-title":"Biochem Pharmacol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10158-010-0105-y","article-title":"Recent progress in understanding the interaction between avermectins and ligand-gated ion channels: putting the pests to sleep","volume":"10","author":"AJ Wolstenholme","year":"2010","journal-title":"Invert Neurosci"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"14890","DOI":"10.1074\/jbc.M110.107789","article-title":"An improved ivermectin-activated chloride channel receptor for inhibiting electrical activity in defined neuronal populations","volume":"285","author":"T Lynagh","year":"2010","journal-title":"J Biol Chem"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0014-2999(00)00164-3","article-title":"Activation of rat recombinant alpha(1)beta(2)gamma(2S) GABA(A) receptor by the insecticide ivermectin","volume":"394","author":"H Adelsberger","year":"2000","journal-title":"Eur J Pharmacol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"12556","DOI":"10.1074\/jbc.M011264200","article-title":"Ivermectin, an unconventional agonist of the glycine receptor chloride channel","volume":"276","author":"Q Shan","year":"2001","journal-title":"Journal of Biological Chemistry"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0097468","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,26]],"date-time":"2024-05-26T17:04:27Z","timestamp":1716743067000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0097468"}},"subtitle":[],"editor":[{"given":"Subhabrata","family":"Sanyal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,5,13]]},"references-count":51,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,5,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0097468","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,13]]}}}