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Geneve: WHO. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/tb\/publications\/global_report\/en\/index.html\" xlink:type=\"simple\">http:\/\/www.who.int\/tb\/publications\/global_report\/en\/index.html<\/ext-link>. 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