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histotechnology, 2nd Ed, Battelle Press, Ohio. 235\u2013237."},{"key":"ref37","unstructured":"Crookham J, Dapson R (1991) Hazardous chemicals in the histopathology laboratory, 2<sup>nd<\/sup> ED, Anatech."},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH (ed.) 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M29\u2013A2."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/S0962-8479(05)80033-4","article-title":"Establishment and evaluation of a multiplex polymerase chain reaction for detection of mycobacteria and specific identification of <italic>Mycobacterium tuberculosis<\/italic> complex","volume":"76(4)","author":"AS Mustafa","year":"1995","journal-title":"Tuber Lung Dis"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1099\/mic.0.25961-0","article-title":"Molecular analysis of <italic>Mycobacterium tuberculosis<\/italic> DNA from a family of 18<sup>th<\/sup> century Hungarians","volume":"149","author":"HA Fletcher","year":"2003","journal-title":"Microbiol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1128\/JCM.39.5.2006-2008.2001","article-title":"Evaluation of PCR using TRC4 and IS6110 primers in detection of tuberculous meningitis","volume":"39","author":"N Sujatha","year":"2001","journal-title":"J 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