{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:38:40Z","timestamp":1791362320368,"version":"4.3.1"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T00:00:00Z","timestamp":1402358400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0098415","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T23:57:52Z","timestamp":1402444672000},"page":"e98415","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":116,"title":["Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-\u03b3 Production from Tumor-Infiltrating Cells by PD-L1 Blockade"],"prefix":"10.1371","volume":"9","author":[{"given":"Naoya","family":"Maekawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Konnai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoyo","family":"Ikebuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Okagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Adachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Kagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Murata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Ohashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,6,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1111\/j.1748-5827.2010.00974.x","article-title":"Methods and mortality results of a health survey of purebred dogs in the UK","volume":"51","author":"VJ Adams","year":"2010","journal-title":"J Small Anim Pract"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nm0902-1039c","article-title":"Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion","volume":"8","author":"H Dong","year":"2002","journal-title":"Nat Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1158\/1078-0432.CCR-12-2063","article-title":"Antagonist antibodies to PD-1 and B7-H1 (PD-L1) in the treatment of advanced human cancer","volume":"19","author":"M Sznol","year":"2013","journal-title":"Clin Cancer Res"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1593\/neo.05733","article-title":"The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors","volume":"8","author":"H Ghebeh","year":"2006","journal-title":"Neoplasia"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s00432-008-0364-8","article-title":"B7-H1 up-regulated expression in human pancreatic carcinoma tissue associates with tumor progression","volume":"134","author":"L Geng","year":"2008","journal-title":"J Cancer Res Clin Oncol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1158\/1078-0432.CCR-06-2746","article-title":"Clinical significance and therapeutic potential of the programmed death-1 ligand\/programmed death-1 pathway in human pancreatic cancer","volume":"13","author":"T Nomi","year":"2007","journal-title":"Clin Cancer Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1002\/cncr.22588","article-title":"PD-L1 (B7-H1) expression by urothelial carcinoma of the bladder and BCG-induced granulomata: associations with localized stage progression","volume":"109","author":"BA Inman","year":"2007","journal-title":"Cancer"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1038\/jid.2011.30","article-title":"Progressive upregulation of PD-1 in primary and metastatic melanomas associated with blunted TCR signaling in infiltrating T lymphocytes","volume":"131","author":"M Chapon","year":"2011","journal-title":"J Invest Dermatol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"5094","DOI":"10.1158\/1078-0432.CCR-04-0428","article-title":"B7-H1 expression on non-small cell lung cancer cells and its relationship with tumor-infiltrating lymphocytes and their PD-1 expression","volume":"10","author":"J Konishi","year":"2004","journal-title":"Clin Cancer Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1002\/jso.21376","article-title":"Interaction of B7-H1 on intrahepatic cholangiocarcinoma cells with PD-1 on tumor-infiltrating T cells as a mechanism of immune evasion","volume":"100","author":"Y Ye","year":"2009","journal-title":"J Surg Oncol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1158\/0008-5472.CAN-05-4303","article-title":"Tumor B7-H1 is associated with poor prognosis in renal cell carcinoma patients with long-term follow-up","volume":"66","author":"RH Thompson","year":"2006","journal-title":"Cancer Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.acthis.2006.01.003","article-title":"Immunohistochemical localization of programmed death-1 ligand-1 (PD-L1) in gastric carcinoma and its clinical significance","volume":"108","author":"C Wu","year":"2006","journal-title":"Acta Histochem"},{"key":"ref13","first-page":"634","article-title":"PD-1: PD-L inhibitory pathway affects both CD4<sup>+<\/sup> and CD8<sup>+<\/sup> T cells and is overcome by IL-2","volume":"32","author":"L Carter","year":"2002","journal-title":"Ear J Imanol"},{"key":"ref14","first-page":"13866","article-title":"PD-1 immunoreceptor inhibits B cell receptor-mediated signaling by recruiting src homology 2-domain-containing tyrosine phosphatase 2 to phosphotyrosine. Proc Natl Acad Sci U S A","volume":"98","author":"T Okazaki","year":"2001"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.febslet.2004.07.083","article-title":"PD-1 inhibits T-cell receptor induced phosphorylation of the ZAP70\/CD3\u03b6 signalosome and downstream signaling to PKCtheta","volume":"574","author":"KA Sheppard","year":"2004","journal-title":"FEBS Lett"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1093\/intimm\/dxm091","article-title":"Programmed death-1 blockade enhances expansion and functional capacity of human melanoma antigen-specific CTLs","volume":"19","author":"RM Wong","year":"2007","journal-title":"Int Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1002\/ijc.25397","article-title":"PD-1 and PD-L1 upregulation promotes CD8+ T-cell apoptosis and postoperative recurrence in hepatocellular carcinoma patients","volume":"128","author":"F Shi","year":"2011","journal-title":"Int J Cancer"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.1056\/NEJMoa1200694","article-title":"Safety and activity of anti-PD-L1 antibody in patients with advanced cancer","volume":"366","author":"JR Brahmer","year":"2012","journal-title":"N Engl J Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1056\/NEJMoa1200690","article-title":"Safety, activity, and immune correlates of anti-PD-1 antibody in cancer","volume":"366","author":"SL Topalian","year":"2012","journal-title":"N Engl J Med"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Ikebuchi R, Konnai S, Okagawa T, Yokoyama K, Nakajima C, et al. Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes. Immunology (in press).","DOI":"10.1111\/imm.12243"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1297-9716-44-59","article-title":"Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells <italic>in vitro<\/italic>","volume":"44","author":"R Ikebuchi","year":"2013","journal-title":"Vet Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cimid.2012.09.005","article-title":"Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus","volume":"36","author":"S Konnai","year":"2013","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1297-9716-43-45","article-title":"Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection","volume":"43","author":"T Okagawa","year":"2012","journal-title":"Vet Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/1297-9716-42-103","article-title":"Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade","volume":"42","author":"R Ikebuchi","year":"2011","journal-title":"Vet Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.vetimm.2011.08.018","article-title":"Molecular cloning of bovine lymphocyte activation gene-3 and its expression characteristics in bovine leukemia virus-infected cattle","volume":"144","author":"T Shirai","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"key":"ref26","first-page":"291","article-title":"Molecular cloning and expression analysis of bovine programmed death-1","volume":"54","author":"R Ikebuchi","year":"2010","journal-title":"Microbiol Immunol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0092-8674(81)90282-8","article-title":"SV40-transformed simian cells support the replication of early SV40 mutants","volume":"23","author":"Y Gluzman","year":"1981","journal-title":"Cell"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1292\/jvms.64.169","article-title":"Effect of natural and synthetic retinoids on the proliferation and differentiation of three canine melanoma cell lines","volume":"64","author":"E Ohashi","year":"2002","journal-title":"J Vet Med Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1292\/jvms.63.83","article-title":"Effect of retinoids on growth inhibition of two canine melanoma cell lines","volume":"63","author":"E Ohashi","year":"2001","journal-title":"J Vet Med Sci"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1159\/000053820","article-title":"IgG-mediated histamine release from canine mastocytoma-derived cells","volume":"125","author":"T Takahashi","year":"2001","journal-title":"Int Arch Allergy Immunol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1292\/jvms.63.1031","article-title":"Establishment and characterization of a new canine mast cell tumor cell line","volume":"63","author":"T Ishiguro","year":"2001","journal-title":"J Vet Med Sci"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1292\/jvms.56.1167","article-title":"Establishment and characterization of a new cell line from a canine osteosarcoma","volume":"56","author":"T Kadosawa","year":"1994","journal-title":"J Vet Med Sci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1292\/jvms.61.361","article-title":"Establishment and characterization of the growth and pulmonary metastasis of a highly lung metastasizing cell line from canine osteosarcoma in nude mice","volume":"61","author":"EF Barroga","year":"1999","journal-title":"J Vet Med Sci"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"K Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"ref35","first-page":"406","article-title":"the neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"N Saitou","year":"1987","journal-title":"Mol Biol Evol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1089\/dna.1990.9.347","article-title":"Expression and characterization of human CD4: immunoglobulin fusion proteins","volume":"9","author":"G Zettlmeissl","year":"1990","journal-title":"DNA Cell Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0378-1119(91)90434-D","article-title":"Efficient selection for high-expression transfectants with a novel eukaryotic vector","volume":"108","author":"H Niwa","year":"1991","journal-title":"Gene"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"945","DOI":"10.4049\/jimmunol.173.2.945","article-title":"SHP-1 and SHP-2 associate with immunoreceptor tyrosine-based switch motif of programmed death 1 upon primary human T cell stimulation, but only receptor ligation prevents T cell activation","volume":"173","author":"JM Chemnitz","year":"2004","journal-title":"J Immunol"},{"key":"ref39","first-page":"3044","article-title":"Phase I safety and pharmacokinetic study of CT-011, a humanized antibody interacting with PD-1, in patients with advanced hematologic malignancies. Clin Cancer Res","volume":"14","author":"R Berger","year":"2008"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/713774061","article-title":"Expression and regulation of the PD-L1 immunoinhibitory molecule on microvascular endothelial cells","volume":"9","author":"MJ Eppihimer","year":"2002","journal-title":"Microcirculation"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.febslet.2005.12.093","article-title":"Interferon regulatory factor-1 is prerequisite to the constitutive expression and IFN-gamma-induced upregulation of B7-H1 (CD274)","volume":"580","author":"SJ Lee","year":"2006","journal-title":"FEBS Lett"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1126\/science.1203486","article-title":"Cancer immunoediting: integrating immunity\u2019s roles in cancer suppression and promotion","volume":"331","author":"RD Schreiber","year":"2011","journal-title":"Science"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1365-3164.2008.00694.x","article-title":"Canine mast cell tumours: a review of the pathogenesis, clinical features, pathology and treatment","volume":"19","author":"MM Welle","year":"2008","journal-title":"Vet Dermatol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.4049\/jimmunol.176.4.2238","article-title":"Mast cells enhance T cell activation: importance of mast cell costimulatory molecules and secreted TNF","volume":"176","author":"S Nakae","year":"2006","journal-title":"J Immunol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1111\/j.1523-1755.2005.00665.x","article-title":"Renal tubular epithelial cells modulate T-cell responses via ICOS-L and B7-H1","volume":"68","author":"S de Haij","year":"2005","journal-title":"Kidney Int"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"580","DOI":"10.2460\/javma.1981.178.06.580","article-title":"Prognostic criteria for dogs with oral melanoma","volume":"178","author":"HJ Harvey","year":"1981","journal-title":"J Am Vet Med Assoc"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1354\/vp.43-2-136","article-title":"the histologic and epidemiologic bases for prognostic considerations in canine melanocytic neoplasia","volume":"43","author":"WL Spangler","year":"2006","journal-title":"Vet Pathos"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1177\/030098589002700204","article-title":"Characteristics of canine melanomas and comparison of histology and DNA policy to their biologic behavior","volume":"27","author":"B Bolton","year":"1990","journal-title":"Vet Pathos"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"388","DOI":"10.2460\/javma.2004.224.388","article-title":"Evaluation of cisplatin combined with piroxicam for the treatment of oral malignant melanoma and oral squamous cell carcinoma in dogs","volume":"224","author":"PA Beria","year":"2004","journal-title":"J Am Vet Med Assoc"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"294","DOI":"10.5326\/0390294","article-title":"Combined dorsolateral and intraoral approach for the resection of tumors of the maxilla in the dog","volume":"39","author":"BD Lascelles","year":"2003","journal-title":"J Am Anim Hosp Assoc"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.2460\/javma.2001.218.1444","article-title":"Use of carboplatin for treatment of dogs with malignant melanoma: 27 cases (1989\u20132000)","volume":"218","author":"KM Rassnick","year":"2001","journal-title":"J Am Vet Med Assoc"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/j.1740-8261.2003.tb00468.x","article-title":"a retrospective analysis of 140 dogs with oral melanoma treated with external beam radiation","volume":"44","author":"DR Proulx","year":"2003","journal-title":"Vet Radial Ultrasound"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0098415","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T09:17:19Z","timestamp":1746263839000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0098415"}},"subtitle":[],"editor":[{"given":"Hiroshi","family":"Shiku","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,10]]},"references-count":52,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0098415","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,10]]}}}