{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:37:07Z","timestamp":1790692627390,"version":"4.1.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T00:00:00Z","timestamp":1401926400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0098953","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T20:47:56Z","timestamp":1402001276000},"page":"e98953","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Acute Sterol O-Acyltransferase 2 (SOAT2) Knockdown Rapidly Mobilizes Hepatic Cholesterol for Fecal Excretion"],"prefix":"10.1371","volume":"9","author":[{"given":"Stephanie M.","family":"Marshall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony D.","family":"Gromovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn L.","family":"Kelley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha D.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosanne M.","family":"Crooke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence L.","family":"Rudel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Mark","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan E.","family":"Temel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,6,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1161\/CIR.0b013e31828124ad","article-title":"Heart disease and stroke statistics\u20142013 update: a report from the American Heart Association","volume":"127","author":"AS Go","year":"2013","journal-title":"Circulation"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1056\/NEJMoa0807646","article-title":"Rosuvastatin to prevent vascular events in men and women with elevated C-reactive protein","volume":"359","author":"PM Ridker","year":"2008","journal-title":"N Engl J Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s40265-013-0028-0","article-title":"LDL cholesterol goals in high-risk patients: how low do we go and how do we get there?","volume":"73","author":"J Besseling","year":"2013","journal-title":"Drugs"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1152\/physrev.1965.45.4.747","article-title":"Cholesterol ester metabolism","volume":"45","author":"DS Goodman","year":"1965","journal-title":"Physiol Rev"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1146\/annurev.biochem.66.1.613","article-title":"Acyl-coenzyme A:cholesterol acyltransferase","volume":"66","author":"TY Chang","year":"1997","journal-title":"Annu Rev Biochem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1161\/01.ATV.0000166548.65753.1e","article-title":"ACAT2 is a target for treatment of coronary heart disease associated with hypercholesterolemia","volume":"25","author":"LL Rudel","year":"2005","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1194\/jlr.M700109-JLR200","article-title":"ACAT2 stimulates cholesteryl ester secretion in apoB-containing lipoproteins","volume":"48","author":"RE Temel","year":"2007","journal-title":"J Lipid Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1194\/jlr.M500232-JLR200","article-title":"Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2","volume":"46","author":"RE Temel","year":"2005","journal-title":"J Lipid Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/hep.20439","article-title":"ACAT2 deficiency limits cholesterol absorption in the cholesterol-fed mouse: impact on hepatic cholesterol homeostasis","volume":"40","author":"JJ Repa","year":"2004","journal-title":"Hepatology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1194\/jlr.M024356","article-title":"Tissue-specific knockouts of ACAT2 reveal that intestinal depletion is sufficient to prevent diet-induced cholesterol accumulation in the liver and blood","volume":"53","author":"J Zhang","year":"2012","journal-title":"J Lipid Res"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1194\/jlr.M018820","article-title":"Cholesterol esterification by ACAT2 is essential for efficient intestinal cholesterol absorption: evidence from thoracic lymph duct cannulation","volume":"53","author":"TM Nguyen","year":"2012","journal-title":"J Lipid Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1194\/jlr.M500018-JLR200","article-title":"ACAT2 contributes cholesteryl esters to newly secreted VLDL, whereas LCAT adds cholesteryl ester to LDL in mice","volume":"46","author":"RG Lee","year":"2005","journal-title":"J Lipid Res"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1194\/jlr.M039644","article-title":"LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis","volume":"54","author":"JT Melchior","year":"2013","journal-title":"J Lipid Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1161\/01.RES.0000147558.15554.67","article-title":"Plasma cholesteryl esters provided by lecithin:cholesterol acyltransferase and acyl-coenzyme a:cholesterol acyltransferase 2 have opposite atherosclerotic potential","volume":"95","author":"RG Lee","year":"2004","journal-title":"Circ Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1073\/pnas.0336398100","article-title":"Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice","volume":"100","author":"EL Willner","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1038\/82153","article-title":"Resistance to diet-induced hypercholesterolemia and gallstone formation in ACAT2-deficient mice","volume":"6","author":"KK Buhman","year":"2000","journal-title":"Nat Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1161\/ATVBAHA.107.142802","article-title":"Dietary fat-induced alterations in atherosclerosis are abolished by ACAT2-deficiency in ApoB100 only, LDLr-\/- mice","volume":"27","author":"TA Bell 3rd","year":"2007","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/S0022-2275(20)30149-8","article-title":"In vivo contribution of LCAT to apolipoprotein B lipoprotein cholesteryl esters in LDL receptor and apolipoprotein E knockout mice","volume":"43","author":"JW Furbee Jr","year":"2002","journal-title":"J Lipid Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1161\/ATVBAHA.111.223552","article-title":"Pyripyropene A, an acyl-coenzyme A:cholesterol acyltransferase 2-selective inhibitor, attenuates hypercholesterolemia and atherosclerosis in murine models of hyperlipidemia","volume":"31","author":"T Ohshiro","year":"2011","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1161\/01.ATV.0000225289.30767.06","article-title":"Liver-specific inhibition of acyl-coenzyme a:cholesterol acyltransferase 2 with antisense oligonucleotides limits atherosclerosis development in apolipoprotein B100-only low-density lipoprotein receptor-\/- mice","volume":"26","author":"TA Bell 3rd","year":"2006","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"10522","DOI":"10.1074\/jbc.M707659200","article-title":"Targeted depletion of hepatic ACAT2-driven cholesterol esterification reveals a non-biliary route for fecal neutral sterol loss","volume":"283","author":"JM Brown","year":"2008","journal-title":"J Biol Chem"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1097\/MOL.0b013e3283508c21","article-title":"Biliary and nonbiliary contributions to reverse cholesterol transport","volume":"23","author":"RE Temel","year":"2012","journal-title":"Curr Opin Lipidol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1161\/ATVBAHA.108.181206","article-title":"Reverse cholesterol transport revisited: contribution of biliary versus intestinal cholesterol excretion","volume":"31","author":"G Brufau","year":"2011","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"14267","DOI":"10.1074\/jbc.M110.118422","article-title":"Inhibition of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) prevents dietary cholesterol-associated steatosis by enhancing hepatic triglyceride mobilization","volume":"285","author":"HM Alger","year":"2010","journal-title":"J Biol Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1161\/01.CIR.0000143163.76212.0B","article-title":"ACAT2 is localized to hepatocytes and is the major cholesterol-esterifying enzyme in human liver","volume":"110","author":"P Parini","year":"2004","journal-title":"Circulation"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/nature10486","article-title":"Inhibition of miR-33a\/b in non-human primates raises plasma HDL and lowers VLDL triglycerides","volume":"478","author":"KJ Rayner","year":"2011","journal-title":"Nature"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"EG Bligh","year":"1959","journal-title":"Can J Biochem Physiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1172\/JCI30060","article-title":"Hepatic Niemann-Pick C1-like 1 regulates biliary cholesterol concentration and is a target of ezetimibe","volume":"117","author":"RE Temel","year":"2007","journal-title":"J Clin Invest"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0009-9120(93)90015-X","article-title":"Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts","volume":"26","author":"TP Carr","year":"1993","journal-title":"Clin Biochem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.cmet.2010.05.011","article-title":"Biliary sterol secretion is not required for macrophage reverse cholesterol transport","volume":"12","author":"RE Temel","year":"2010","journal-title":"Cell Metab"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.cmet.2010.02.005","article-title":"Loss of TGH\/Ces3 in Mice Decreases Blood Lipids, Improves Glucose Tolerance, and Increases Energy Expenditure","volume":"11","author":"E Wei","year":"2010","journal-title":"Cell Metab"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1023\/B:REMD.0000045101.42431.c7","article-title":"The physiological role of triacylglycerol hydrolase in lipid metabolism","volume":"5","author":"D Gilham","year":"2004","journal-title":"Rev Endocr Metab Disord"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1161\/ATVBAHA.112.301041","article-title":"Physiological difference in autophagic flux in macrophages from 2 mouse strains regulates cholesterol ester metabolism","volume":"33","author":"P Robinet","year":"2013","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.cmet.2011.03.023","article-title":"Autophagy regulates cholesterol efflux from macrophage foam cells via lysosomal acid lipase","volume":"13","author":"M Ouimet","year":"2011","journal-title":"Cell Metab"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1172\/JCI200523915","article-title":"Targeted inactivation of hepatic Abca1 causes profound hypoalphalipoproteinemia and kidney hypercatabolism of apoA-I","volume":"115","author":"JM Timmins","year":"2005","journal-title":"J Clin Invest"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1194\/jlr.M022194","article-title":"Trans-intestinal cholesterol efflux is not mediated through high density lipoprotein","volume":"53","author":"CL Vrins","year":"2012","journal-title":"J Lipid Res"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"Le May C, Berger JM, Lespine A, Pillot B, Prieur X, et al. (2013) Transintestinal Cholesterol Excretion Is an Active Metabolic Process Modulated by PCSK9 and Statin Involving ABCB1. Arterioscler Thromb Vasc Biol.","DOI":"10.1161\/ATVBAHA.112.300263"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"49072","DOI":"10.1074\/jbc.M304274200","article-title":"Enlargement of high density lipoprotein in mice via liver X receptor activation requires apolipoprotein E and is abolished by cholesteryl ester transfer protein expression","volume":"278","author":"XC Jiang","year":"2003","journal-title":"J Biol Chem"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"19211","DOI":"10.1074\/jbc.M109.014860","article-title":"Activation of the liver X receptor stimulates trans-intestinal excretion of plasma cholesterol","volume":"284","author":"JN van der Veen","year":"2009","journal-title":"J Biol Chem"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0098953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T04:53:54Z","timestamp":1649220834000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0098953"}},"subtitle":[],"editor":[{"given":"Antonio","family":"Moschetta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,5]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0098953","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,5]]}}}