{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T01:07:14Z","timestamp":1778116034314,"version":"3.51.4"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T00:00:00Z","timestamp":1403222400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0099853","type":"journal-article","created":{"date-parts":[[2014,6,20]],"date-time":"2014-06-20T20:21:44Z","timestamp":1403295704000},"page":"e99853","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Pleiotropic Effect of AccD5 and AccE5 Depletion in Acyl-Coenzyme A Carboxylase Activity and in Lipid Biosynthesis in Mycobacteria"],"prefix":"10.1371","volume":"9","author":[{"given":"Bernardo","family":"Bazet Lyonnet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lautaro","family":"Diacovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00edas","family":"Cabruja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabienne","family":"Bardou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna\u00efk","family":"Qu\u00e9mard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Gago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo","family":"Gramajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,6,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1056\/NEJMra1200894","article-title":"Tuberculosis","volume":"368","author":"A Zumla","year":"2013","journal-title":"New Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S195","DOI":"10.1086\/651491","article-title":"Extensively drug-resistant tuberculosis: \u201cthere must be some kind of way out of here\u201d","volume":"50 Suppl 3","author":"JP Cegielski","year":"2010","journal-title":"Clin Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"S260","DOI":"10.1086\/651500","article-title":"From HIV to tuberculosis and back again: a tale of activism in 2 pandemics","volume":"50 Suppl 3","author":"M Harrington","year":"2010","journal-title":"Clin Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Daffe M (2008) The global architecture of the mycobacteria cell envelope. In The Mycobacterial Cell Envelope. Daffe M and Reyrat JM (eds). Washington, DC: ASM Press, pp. 3\u201312.","DOI":"10.1128\/9781555815783.ch1"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1146\/annurev.bi.64.070195.000333","article-title":"The envelope of mycobacteria","volume":"64","author":"PJ Brennan","year":"1995","journal-title":"Annu Rev Biochem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1046\/j.1365-2958.2000.01882.x","article-title":"Oxygenated mycolic acids are necessary for virulence of <italic>Mycobacterium tuberculosis<\/italic> in mice","volume":"36","author":"E Dubnau","year":"2000","journal-title":"Mol Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/S1097-2765(00)80250-6","article-title":"A novel mycolic acid cyclopropane synthetase is required for cording, persistence, and virulence of <italic>Mycobacterium tuberculosis<\/italic>","volume":"5","author":"MS Glickman","year":"2000","journal-title":"Mol Cell"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1172\/JCI27335","article-title":"Trans-cyclopropanation of mycolic acids on trehalose dimycolate suppresses <italic>Mycobacterium tuberculosis<\/italic> -induced inflammation and virulence","volume":"116","author":"V Rao","year":"2006","journal-title":"J Clin Invest"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1111\/j.1365-2958.2007.05761.x","article-title":"The <italic>Mycobacterium tuberculosis<\/italic> FAS-II condensing enzymes: their role in mycolic acid biosynthesis, acid-fastness, pathogenesis and in future drug development","volume":"64","author":"A Bhatt","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"5157","DOI":"10.1073\/pnas.0608654104","article-title":"Deletion of <italic>kasB<\/italic> in <italic>Mycobacterium tuberculosis<\/italic> causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice","volume":"104","author":"A Bhatt","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.chembiol.2013.11.011","article-title":"Mycolic acids: structures, biosynthesis, and beyond","volume":"21","author":"H Marrakchi","year":"2014","journal-title":"Chem Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"31228","DOI":"10.1074\/jbc.M203263200","article-title":"Kinetic and structural analysis of a new group of Acyl-CoA carboxylases found in <italic>Streptomyces coelicolor<\/italic> A3(2)","volume":"277","author":"L Diacovich","year":"2002","journal-title":"J Biol Chem"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1007\/s00018-005-5121-4","article-title":"Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery","volume":"62","author":"L Tong","year":"2005","journal-title":"Cell MolLife Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1574-6976.2010.00259.x","article-title":"Fatty acid biosynthesis in actinomycetes","volume":"35","author":"G Gago","year":"2011","journal-title":"FEMS Microbiol Rev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0163-7827(02)00007-3","article-title":"Multi-subunit acetyl-CoA carboxylases","volume":"41","author":"JE Cronan Jr","year":"2002","journal-title":"Prog Lipid Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1365-2958.2010.07274.x","article-title":"FasR, a novel class of transcriptional regulator, governs the activation of fatty acid biosynthesis genes in <italic>Streptomyces coelicolor<\/italic>","volume":"78","author":"A Arabolaza","year":"2010","journal-title":"Mol Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1128\/JB.188.2.477-486.2006","article-title":"Biochemical and structural characterization of an essential acyl coenzyme A carboxylase from <italic>Mycobacterium tuberculosis<\/italic>","volume":"188","author":"G Gago","year":"2006","journal-title":"J Bacteriol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/31159","article-title":"Deciphering the biology of <italic>Mycobacterium tuberculosis<\/italic> from the complete genome sequence","volume":"393","author":"ST Cole","year":"1998","journal-title":"Nature"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1074\/jbc.M511761200","article-title":"Identification and characterization of Rv3281 as a novel subunit of a biotin-dependent acyl-CoA Carboxylase in <italic>Mycobacterium tuberculosis<\/italic> H37Rv","volume":"281","author":"TJ Oh","year":"2006","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1128\/JB.01019-06","article-title":"AccD6, a member of the Fas II locus, is a functional carboxyltransferase subunit of the acyl-coenzyme A carboxylase in <italic>Mycobacterium tuberculosis<\/italic>","volume":"189","author":"J Daniel","year":"2007","journal-title":"J Bacteriol"},{"key":"ref21","article-title":"Accase 6 is the essential acetyl-CoA carboxylase involved in fatty acid and mycolic acid biosynthesis in mycobacteria","author":"DG Kurth","year":"2009","journal-title":"Microbiology"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6960","DOI":"10.1128\/JB.05638-11","article-title":"AccD6, a key carboxyltransferase essential for mycolic acid synthesis in <italic>Mycobacterium tuberculosis<\/italic>, is dispensable in a nonpathogenic strain","volume":"193","author":"J Pawelczyk","year":"2011","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"8862","DOI":"10.1074\/jbc.M408578200","article-title":"The acyl-AMP ligase FadD32 and AccD4-containing acyl-CoA carboxylase are required for the synthesis of mycolic acids and essential for mycobacterial growth: identification of the carboxylation product and determination of the acyl-CoA carboxylase components","volume":"280","author":"D Portevin","year":"2005","journal-title":"J Biol Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1073\/pnas.0305439101","article-title":"A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms","volume":"101","author":"D Portevin","year":"2004","journal-title":"Proc Natl Acad SciUSA"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0022-2836(83)80284-8","article-title":"Studies on transformation of <italic>Escherichia coli<\/italic> with plasmids","volume":"166","author":"D Hanahan","year":"1983","journal-title":"J Mol Biol"},{"key":"ref26","unstructured":"Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1111\/j.1365-2958.1990.tb02040.x","article-title":"Isolation and characterization of efficient plasmid transformation mutants of <italic>Mycobacterium smegmatis<\/italic>","volume":"4","author":"SB Snapper","year":"1990","journal-title":"Mol Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0091-679X(08)61848-8","article-title":"<italic>Mycobacterium<\/italic>: isolation, maintenance, transformation, and mutant selection","volume":"45","author":"ND Connell","year":"1994","journal-title":"Methods Cell Biol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.jbiotec.2009.02.001","article-title":"Pristinamycin-inducible gene regulation in mycobacteria","volume":"140","author":"F Forti","year":"2009","journal-title":"J Biotechnol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ab.2005.10.006","article-title":"Quantification of the concentration and 13C tracer enrichment of long-chain fatty acyl-coenzyme A in muscle by liquid chromatography\/mass spectrometry","volume":"349","author":"D Sun","year":"2006","journal-title":"Anal Biochem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"16857","DOI":"10.1074\/jbc.M000569200","article-title":"Thiolactomycin and related analogues as novel anti-mycobacterial agents targeting KasA and KasB condensing enzymes in <italic>Mycobacterium tuberculosis<\/italic>","volume":"275","author":"L Kremer","year":"2000","journal-title":"J Biol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1073\/pnas.0510580103","article-title":"Structure-based inhibitor design of AccD5, an essential acyl-CoA carboxylase carboxyltransferase domain of <italic>Mycobacterium tuberculosis<\/italic>","volume":"103","author":"TW Lin","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1046\/j.1365-2958.2003.03425.x","article-title":"Genes required for mycobacterial growth defined by high density mutagenesis","volume":"48","author":"CM Sassetti","year":"2003","journal-title":"Mol Microbiol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e1002251","DOI":"10.1371\/journal.ppat.1002251","article-title":"High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism","volume":"7","author":"JE Griffin","year":"2011","journal-title":"PLoS Pathog"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"10955","DOI":"10.1073\/pnas.94.20.10955","article-title":"Efficient allelic exchange and transposon mutagenesis in <italic>Mycobacterium tuberculosis<\/italic>","volume":"94","author":"V Pelicic","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/j.1574-6968.2004.tb09547.x","article-title":"Use of a novel allele of the <italic>Escherichia coli aacC4<\/italic> aminoglycoside resistance gene as a genetic marker in mycobacteria","volume":"234","author":"SA Consaul","year":"2004","journal-title":"FEMS Microbiol Lett"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e134","DOI":"10.1093\/nar\/gkq235","article-title":"Development of a repressible mycobacterial promoter system based on two transcriptional repressors","volume":"38","author":"F Boldrin","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1128\/JB.00254-07","article-title":"The two carboxylases of <italic>Corynebacterium glutamicum<\/italic> essential for fatty acid and mycolic acid synthesis","volume":"189","author":"R Gande","year":"2007","journal-title":"J Bacteriol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/j.jmb.2005.09.016","article-title":"The biosynthesis of mycolic acids in <italic>Mycobacterium tuberculosis<\/italic> relies on multiple specialized elongation complexes interconnected by specific protein-protein interactions","volume":"353","author":"R Veyron-Churlet","year":"2005","journal-title":"J Mol Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1111\/j.1365-2958.2004.04334.x","article-title":"Protein-protein interactions within the Fatty Acid Synthase-II system of <italic>Mycobacterium tuberculosis<\/italic> are essential for mycobacterial viability","volume":"54","author":"R Veyron-Churlet","year":"2004","journal-title":"Mol Microbiol"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0242528","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T00:00:00Z","timestamp":1605052800000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0099853","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T22:54:02Z","timestamp":1716850442000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0099853"}},"subtitle":[],"editor":[{"given":"Riccardo","family":"Manganelli","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,20]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0099853","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,20]]}}}