{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:32:43Z","timestamp":1792521163832,"version":"4.5.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,27]],"date-time":"2014-06-27T00:00:00Z","timestamp":1403827200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0100453","type":"journal-article","created":{"date-parts":[[2014,6,27]],"date-time":"2014-06-27T20:22:38Z","timestamp":1403900558000},"page":"e100453","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":58,"title":["Multilocus Genotyping of Giardia duodenalis in Dairy Cattle in Henan, China"],"prefix":"10.1371","volume":"9","author":[{"given":"Haiyan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongyi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuchun","family":"Jian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiju","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,6,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.exppara.2009.03.001","article-title":"Is <italic>Giardia<\/italic> a significant pathogen in production animals?","volume":"124","author":"T Geurden","year":"2010","journal-title":"Exp Parasitol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.pt.2006.02.009","article-title":"Tools for investigating the environmental transmission of <italic>Cryptosporidium<\/italic> and <italic>Giardia<\/italic> infections in humans","volume":"22","author":"H Smith","year":"2006","journal-title":"Trends Parasitol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2004.09.008","article-title":"The zoonotic significance and molecular epidemiology of <italic>Giardi<\/italic> and giardiasis","volume":"126","author":"RC Thompson","year":"2004","journal-title":"Vet Parasitol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S1567-1348(02)00149-1","article-title":"Genetic diversity within the morphological species <italic>Giardia intestinalis<\/italic> and its relationship to host origin","volume":"3","author":"PT Monis","year":"2003","journal-title":"Infect Genet Evol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.ijpara.2010.02.015","article-title":"The identification of a new <italic>Giardia duodenalis<\/italic> assemblage in marine vertebrates and a preliminary analysis of <italic>G. duodenalis<\/italic> population biology in marine systems","volume":"40","author":"E Lasek-Nesselquist","year":"2010","journal-title":"Int J Parasitol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1017\/S0031182000064556","article-title":"Division of <italic>Giardia<\/italic> isolates from humans into two genetically distinct assemblages by electrophoretic analysis of enzymes encoded at 27 loci and comparison with <italic>Giardia muris<\/italic>","volume":"111","author":"G Mayrhofer","year":"1995","journal-title":"Parasitology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"e558","DOI":"10.1371\/journal.pntd.0000558","article-title":"Identification of zoonotic genotypes of <italic>Giardia duodenalis<\/italic>","volume":"3","author":"H Sprong","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ijpara.2007.07.016","article-title":"Mixed <italic>Giardia duodenalis<\/italic> assemblage A and E infections in calves","volume":"38","author":"T Geurden","year":"2008","journal-title":"Int J Parasitol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetpar.2005.03.032","article-title":"Prevalence and genotypes of <italic>Giardia duodenalis<\/italic> in post-weaned dairy calves","volume":"130","author":"JM Trout","year":"2005","journal-title":"Vet Parasitol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0304-4017(02)00422-3","article-title":"Prevalence and genotyping of <italic>Giardia duodenalis<\/italic> from beef calves in Alberta, Canada","volume":"112","author":"AJ Appelbee","year":"2003","journal-title":"Vet Parasitol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1017\/S0031182006001533","article-title":"Molecular and phylogenetic characterization of <italic>Cryptosporidium<\/italic> and <italic>Giardia<\/italic> from pigs and cattle in Denmark","volume":"34","author":"RB Langkjaer","year":"2007","journal-title":"Parasitol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2004.05.020","article-title":"Molecular epidemiology of <italic>Giardia<\/italic> and <italic>Cryptosporidium<\/italic> infections in dairy calves originating from three sources in Western Australia","volume":"123","author":"KA Becher","year":"2004","journal-title":"Vet Parasitol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetpar.2007.03.019","article-title":"Molecular characterization of <italic>Cryptosporidium<\/italic> and <italic>Giardia<\/italic> isolates from cattle from Portugal","volume":"147","author":"C Mendonca","year":"2007","journal-title":"Vet Parasitol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijpara.2004.10.022","article-title":"Genetic heterogeneity at the beta-giardin locus among human and animal isolates of <italic>Giardia duodenalis<\/italic> and identification of potentially zoonotic subgenotypes","volume":"35","author":"M Lalle","year":"2005","journal-title":"Int J Parasitol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetpar.2007.09.014","article-title":"Prevalence and molecular characterization of <italic>Giardia duodenalis<\/italic> and <italic>Cryptosporidium<\/italic> spp. in dairy cattle in Ontario, Canada","volume":"150","author":"T Coklin","year":"2007","journal-title":"Vet Parasitol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5100","DOI":"10.1128\/AEM.00232-08","article-title":"Molecular characterization of <italic>Giardia<\/italic> isolates from calves and humans in a region in which dairy farming has recently intensified","volume":"74","author":"CL Winkworth","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.exppara.2010.06.034","article-title":"Genetic characterizations of <italic>Cryptosporidium<\/italic> spp. and <italic>Giardia duodenalis<\/italic> in humans in Henan, China","volume":"127","author":"R Wang","year":"2011","journal-title":"Exp Parasitol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e31262","DOI":"10.1371\/journal.pone.0031262","article-title":"<italic>Cryptosporidium cuniculus<\/italic> and <italic>Giardia duodenalis<\/italic> in rabbits: genetic diversity and possible zoonotic transmission","volume":"7","author":"W Zhang","year":"2012","journal-title":"PLoS One"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.vetpar.2012.04.004","article-title":"Genotype identification and prevalence of <italic>Giardia duodenalis<\/italic> in pet dogs of Guangzhou, Southern China","volume":"188","author":"J Li","year":"2012","journal-title":"Vet Parasitol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.exppara.2013.07.009","article-title":"Molecular characterization of <italic>Giardia duodenalis<\/italic> isolates from police and farm dogs in China","volume":"135","author":"W Li","year":"2013","journal-title":"Exp Parasitol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.parint.2013.10.007","article-title":"Occurrence of human-pathogenic <italic>Enterocytozoon bieneusi, Giardia duodenalis<\/italic> and <italic>Cryptosporidium<\/italic> genotypes in laboratory macaques in Guangxi, China","volume":"63","author":"J Ye","year":"2014","journal-title":"Parasitol Int"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.3201\/eid1810.120653","article-title":"Anthroponotic enteric parasites in monkeys in public park, China","volume":"18","author":"J Ye","year":"2012","journal-title":"Emerg Infect Dis"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e1826","DOI":"10.1371\/journal.pntd.0001826","article-title":"Genetic characterizations of <italic>Giardia duodenalis<\/italic> in sheep and goats in Heilongjiang Province, China and possibility of zoonotic transmission","volume":"6","author":"W Zhang","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s00436-012-2883-0","article-title":"Occurrence of bovine giardiasis and endemic genetic characterization of <italic>Giardia duodenalis<\/italic> isolates in Heilongjiang Province, in the Northeast of China","volume":"111","author":"A Liu","year":"2012","journal-title":"Parasitol Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/S0020-7519(02)00068-1","article-title":"Sequence analysis of the <italic>\u03b2<\/italic>-giardin gene and development of a PCR-RFLP assay to genotype <italic>Giardia duodenalis<\/italic> cysts from human faecal samples","volume":"32","author":"SM Caccio","year":"2002","journal-title":"Int J Parasitol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008","article-title":"Multilocus genotyping of <italic>Giardia duodenalis<\/italic> reveals striking differences between assemblages A and B","volume":"38","author":"SM Caccio","year":"2008","journal-title":"Int J Parasitol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.3201\/eid0911.030084","article-title":"Triosephosphate isomerase gene characterization and potential zoonotic transmission of <italic>Giardia duodenalis<\/italic>","volume":"9","author":"IM Sulaiman","year":"2003","journal-title":"Emerg Infect Dis"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"11030","DOI":"10.1073\/pnas.0404206101","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"K Tamura","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"K Tamura","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.vetpar.2004.07.010","article-title":"Prevalence of <italic>Giardia duodenalis<\/italic> genotypes in pre-weaned dairy calves","volume":"124","author":"JM Trout","year":"2004","journal-title":"Vet Parasitol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/j.1439-0450.2006.00946.x","article-title":"Prevalence and intensity of infection of <italic>Cryptosporidium<\/italic> spp. and <italic>Giardia duodenalis<\/italic> in dairy cattle in Galicia (NW Spain)","volume":"53","author":"JA Castro-Hermida","year":"2006","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.vetpar.2008.01.031","article-title":"Parasitic infections in dairy cattle around Hanoi, northern Vietnam","volume":"31","author":"T Geurden","year":"2008","journal-title":"Vet Parasitol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/00480169.2008.36799","article-title":"Prevalence of <italic>Giardia<\/italic> and <italic>Cryptosporidium<\/italic> spp. in calves from a region in New Zealand experiencing intensification of dairying","volume":"56","author":"CL Winkworth","year":"2008","journal-title":"N Z Vet J"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0304-4017(96)01015-1","article-title":"Prevalence of <italic>Cryptosporidium<\/italic> and <italic>Giardia<\/italic> infections in cattle in Aragon (northeastern Spain)","volume":"66","author":"J Quilez","year":"1996","journal-title":"Vet Parasitol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0304-4017(99)00228-9","article-title":"Epidemiologic study of <italic>Giardia<\/italic> sp. infection in dairy cattle in southeastern New York State","volume":"89","author":"SE Wade","year":"2000","journal-title":"Vet Parasitol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4017(00)00337-X","article-title":"Prevalence of <italic>Giardia<\/italic> sp. <italic>Cryptosporidium parvum<\/italic> and <italic>Cryptosporidium andersoni<\/italic> (syn. <italic>C. muris<\/italic>) [correction of <italic>Cryptosporidium parvum<\/italic> and <italic>Cryptosporidium muris<\/italic> (<italic>C. andersoni<\/italic>)] in 109 dairy herds in five counties of southeastern New York","volume":"93","author":"SE Wade","year":"2000","journal-title":"Vet Parasitol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.vetpar.2011.09.030","article-title":"Prevalence of <italic>Giardia duodenalis<\/italic> assemblages among dairy herds in the New York City Watershed","volume":"185","author":"MP Mark-Carew","year":"2012","journal-title":"Vet Parasitol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0304-4017(01)00514-3","article-title":"Epidemiology of <italic>Cryptosporidium<\/italic> spp. and <italic>Giardia duodenalis<\/italic> on a dairy farm","volume":"102","author":"RE Huetink","year":"2001","journal-title":"Vet Parasitol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"391","DOI":"10.2460\/javma.1999.214.03.391","article-title":"Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea","volume":"214","author":"RM O'Handley","year":"1999","journal-title":"J Am Vet Med Assoc"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.meegid.2004.02.001","article-title":"Discrimination of all genotypes of <italic>Giardia duodenalis<\/italic> at the glutamate dehydrogenase locus using PCR-RFLP","volume":"4","author":"CM Read","year":"2004","journal-title":"Infect Genet Evol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.rvsc.2011.12.012","article-title":"Multilocus genotyping of <italic>Giardia duodenalis<\/italic> in lambs from Spain reveals a high heterogeneity","volume":"93","author":"MT Gomez-Munoz","year":"2012","journal-title":"Res Vet Sci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.vetpar.2004.03.020","article-title":"Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves","volume":"122","author":"M Sant\u00edn","year":"2004","journal-title":"Vet Parasitol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.vetpar.2009.02.008","article-title":"A longitudinal study of <italic>Giardia duodenalis<\/italic> genotypes in dairy cows from birth to 2 years of age","volume":"162","author":"M Santin","year":"2009","journal-title":"Vet Parasitol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.vetpar.2009.11.003","article-title":"From mouse to moose: multilocus genotyping of <italic>Giardia<\/italic> isolates from various animal species","volume":"168","author":"M Lebbad","year":"2010","journal-title":"Vet Parasitol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"e1262","DOI":"10.1371\/journal.pntd.0001262","article-title":"Multilocus genotyping of human <italic>Giardia<\/italic> isolates suggests limited zoonotic transmission and association between assemblage B and flatulence in children","volume":"5","author":"M Lebbad","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.ijpara.2008.03.006","article-title":"Molecular characterisation of species and genotypes of <italic>Cryptosporidium<\/italic> and <italic>Giardia<\/italic> and assessment of zoonotic transmission","volume":"38","author":"L Xiao","year":"2008","journal-title":"Int J Parasitol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e2437","DOI":"10.1371\/journal.pntd.0002437","article-title":"Concurrent infections of <italic>Giardia duodenalis<\/italic>, <italic>Enterocytozoon bieneusi<\/italic>, and <italic>Clostridium difficile<\/italic> in children during a cryptosporidiosis outbreak in a pediatric hospital in China","volume":"7","author":"L Wang","year":"2013","journal-title":"PLoS Negl Trop Dis"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0100453","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T03:07:16Z","timestamp":1716865636000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0100453"}},"subtitle":[],"editor":[{"given":"Yung-Fu","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,27]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0100453","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,27]]}}}