{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:02:00Z","timestamp":1781967720045,"version":"3.54.5"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T00:00:00Z","timestamp":1403049600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0100490","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T20:07:24Z","timestamp":1403122044000},"page":"e100490","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":42,"title":["Effects of Cationic Antimicrobial Peptides on Liquid-Preserved Boar Spermatozoa"],"prefix":"10.1371","volume":"9","author":[{"given":"Martin","family":"Schulze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Junkes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Speck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Ruediger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margitta","family":"Dathe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,6,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1002\/jps.21053","article-title":"Research advances in the development of peptide antibiotics","volume":"97","author":"LM Rossi","year":"2008","journal-title":"J Pharm Sci"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2174\/1568005024605855","article-title":"Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics","volume":"2","author":"RE Hancock","year":"2002","journal-title":"Curr Drug Targets Infect Disord"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e66557","DOI":"10.1371\/journal.pone.0066557","article-title":"LAMP: A Database Linking Antimicrobial Peptides","volume":"8","author":"X Zhao","year":"2013","journal-title":"PLoS One"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0005-2736(99)00198-4","article-title":"Diversity of antimicrobial peptides and their mechanisms of action","volume":"1462","author":"RM Epand","year":"1999","journal-title":"Biochim Biophys Acta"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2174\/1386207053764576","article-title":"Molecular mechanisms of membrane perturbation by antimicrobial peptides and the use of biophysical studies in the design of novel peptide antibiotics","volume":"8","author":"K Lohner","year":"2005","journal-title":"Comb Chem High Throughput Screen"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj2940001","article-title":"Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement","volume":"294","author":"A Zachowski","year":"1993","journal-title":"Biochem J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1002\/bip.20286","article-title":"Antimicrobial peptides: new candidates in the fight against bacterial infections","volume":"80","author":"O Toke","year":"2005","journal-title":"Biopolymers"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0010-7824(96)00038-8","article-title":"Spermicidal activity of Magainins: <italic>in vitro<\/italic> and <italic>in vivo<\/italic> studies","volume":"53","author":"KV Reddy","year":"1996","journal-title":"Contraception"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.fertnstert.2003.09.073","article-title":"Preclinical evaluation of magainin-A as a contraceptive antimicrobial agent","volume":"81","author":"A Clara","year":"2004","journal-title":"Fertil Steril"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0014-5793(01)02648-5","article-title":"Optimization of the antimicrobial activity of magainin peptides by modification of charge","volume":"501","author":"M Dathe","year":"2001","journal-title":"FEBS Letters"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/j.bbamem.2006.04.006","article-title":"Tryptophan- and arginine-rich antimicrobial peptides: structures and mechanisms of action","volume":"1758","author":"DI Chan","year":"2006","journal-title":"Biochim Biophys Acta"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"9140","DOI":"10.1021\/bi035948v","article-title":"Cyclization increases the antimicrobial activity and selectivity of arginine- and tryptophan-containing hexapeptides","volume":"43","author":"M Dathe","year":"2004","journal-title":"Biochemistry"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s00249-011-0671-x","article-title":"Cyclic antimicrobial R-, W-rich peptides: the role of peptide structure and <italic>E. coli<\/italic> outer and inner membranes in activity and the mode of action","volume":"40","author":"C Junkes","year":"2011","journal-title":"Eur Biophys J"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1002\/cbic.200500095","article-title":"Structure of the antimicrobial, cationic hexapeptide cyclo(RRWWRF) and its analogues in solution and bound to detergent micelles","volume":"6","author":"C Appelt","year":"2005","journal-title":"Chembiochem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1002\/psc.940","article-title":"The interaction of arginine- and tryptophan-rich cyclic hexapeptides with <italic>Escherichia coli<\/italic> membranes","volume":"14","author":"C Junkes","year":"2008","journal-title":"J Pept Sci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.theriogenology.2013.07.026","article-title":"Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions","volume":"80","author":"M Schulze","year":"2013","journal-title":"Theriogenology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.anireprosci.2013.05.012","article-title":"Development of an <italic>in vitro<\/italic> index to characterize fertilizing capacity of boar ejaculates","volume":"140","author":"M Schulze","year":"2013","journal-title":"Anim Reprod Sci"},{"key":"ref18","first-page":"161","article-title":"Synthesis and use of spin-labeled lipids for studies of the transmembrane movement of phospholipids","volume":"27","author":"P Fellmann","year":"1994","journal-title":"Methods Mol Biol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-1-4615-5913-9_45","article-title":"The use of spin-labeled phospholipid analogues to characterize the transverse distribution of phospholipids and the activity of phospholipase-A2 in the cell membrane of bull spermatozoa","volume":"424","author":"K Muller","year":"1997","journal-title":"Adv Exp Med Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1095\/biolreprod.107.064253","article-title":"Characterization of sperm plasma membrane properties after cholesterol modification: consequences for cryopreservation of rainbow trout spermatozoa","volume":"78","author":"K Muller","year":"2008","journal-title":"Biol Reprod"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1016\/S0006-291X(77)80146-0","article-title":"Use of spin label tempamine for measuring the interval viscosity of red blood cells","volume":"77","author":"PD Morse","year":"1977","journal-title":"Biochem Biophys Res Commun"},{"key":"ref22","unstructured":"CLSI (2008) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard. 3<sup>rd<\/sup> ed. M31-A3. Vol.28 No.8. Wayne Pennsylvania: Clinical and Laboratory Standards Institute. 99p."},{"key":"ref23","unstructured":"Conover WJ (1999) Some methods based on ranks. Practical nonparametric statistics. 3rd ed. New York: John Wiley &amp; Sons. 367\u2013387."},{"key":"ref24","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.theriogenology.2004.09.031","article-title":"Bacteriospermia in extended porcine semen","volume":"63","author":"GC Althouse","year":"2005","journal-title":"Theriogenology"},{"key":"ref25","unstructured":"Yanagimachi R (1988) Mammalien Fertilization. The Physiology of Reproduction. New York: Raven Press. 135\u2013185."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"6124","DOI":"10.1021\/bi9619987","article-title":"Peptide hydrophobicity controls the activity and selectivity of magainin 2 amide in interaction with membranes","volume":"36","author":"T Wieprecht","year":"1997","journal-title":"Biochemistry"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"9968","DOI":"10.1021\/bi00199a020","article-title":"Protein-dependent translocation of aminophospholipids and asymmetric transbilayer distribution of phospholipids in the plasma membrane of ram sperm cells","volume":"33","author":"K Muller","year":"1994","journal-title":"Biochemistry"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1128\/AAC.01098-10","article-title":"Interaction of W-substituted analogs of cyclo-RRRWFW with bacterial lipopolysaccharides: the role of the aromatic cluster in antimicrobial activity","volume":"55","author":"M Bagheri","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1093\/humrep\/12.5.1006","article-title":"Quantitative observations of flagellar motility of capacitating human spermatozoa","volume":"12","author":"ST Mortimer","year":"1997","journal-title":"Hum Reprod"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1095\/biolreprod59.6.1328","article-title":"Cyclodextrin removes cholesterol from mouse sperm and induces capacitation in a protein-free medium","volume":"59","author":"YH Choi","year":"1998","journal-title":"Biol Reprod"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1530\/rep.1.00561","article-title":"Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction","volume":"130","author":"A Kurz","year":"2005","journal-title":"Reproduction"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1095\/biolreprod.103.026435","article-title":"Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa","volume":"71","author":"S Shadan","year":"2004","journal-title":"Biol Reprod"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1530\/rep.1.01049","article-title":"Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm","volume":"131","author":"K Waterhouse","year":"2006","journal-title":"Reproduction"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"779","DOI":"10.2527\/jas.2011-4311","article-title":"Application of computer assisted semen analysis to explain variations in pig fertility","volume":"90","author":"MLWJ Broekhuijse","year":"2012","journal-title":"J Anim Sci"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0014-5793(91)81191-A","article-title":"Magainins affect respiratory control, membrane potential and motility of hamster spermatozoa","volume":"293","author":"A de Waal","year":"1991","journal-title":"FEBS Letters"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0100490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:37:18Z","timestamp":1588963038000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0100490"}},"subtitle":[],"editor":[{"given":"Axel","family":"Cloeckaert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,18]]},"references-count":35,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0100490","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,18]]}}}