{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T03:40:34Z","timestamp":1778557234651,"version":"3.51.4"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2014,6,30]],"date-time":"2014-06-30T00:00:00Z","timestamp":1404086400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0100863","type":"journal-article","created":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T16:19:07Z","timestamp":1404231547000},"page":"e100863","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Sex Hormones Regulate Tenofovir-Diphosphate in Female Reproductive Tract Cells in Culture"],"prefix":"10.1371","volume":"9","author":[{"given":"Zheng","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John V.","family":"Fahey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack E.","family":"Bodwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Rodriguez-Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela D. M.","family":"Kashuba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles R.","family":"Wira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,6,30]]},"reference":[{"key":"ref1","unstructured":"UNAIDS (2013) UNAIDS report on the global AIDS."},{"key":"ref2","unstructured":"CAC (2003) HIV\/AIDS Surveillance Report."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/aji.12027","article-title":"Immunobiology of genital tract trauma: endocrine regulation of HIV acquisition in women following sexual assault or genital tract mutilation","volume":"69","author":"M Ghosh","year":"2013","journal-title":"Am J Reprod Immunol"},{"key":"ref4","unstructured":"(2012) HIV\/AIDS."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1097\/QAD.0b013e3283060ea4","article-title":"A new strategy to understand how HIV infects women: identification of a window of vulnerability during the menstrual cycle","volume":"22","author":"CR Wira","year":"2008","journal-title":"AIDS"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.jri.2011.01.005","article-title":"Innate and adaptive immunity at mucosal surfaces of the female reproductive tract: stratification and integration of immune protection against the transmission of sexually transmitted infections","volume":"88","author":"DK Hickey","year":"2011","journal-title":"J Reprod Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/j.0105-2896.2005.00287.x","article-title":"Innate and adaptive immunity in female genital tract: cellular responses and interactions","volume":"206","author":"CR Wira","year":"2005","journal-title":"Immunol Rev"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"a007419","DOI":"10.1101\/cshperspect.a007419","article-title":"HIV Prevention by Oral Preexposure Prophylaxis","volume":"2","author":"W Heneine","year":"2012","journal-title":"Cold Spring Harb Perspect Med"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e9310","DOI":"10.1371\/journal.pone.0009310","article-title":"In vitro and ex vivo testing of tenofovir shows it is effective as an HIV-1 microbicide","volume":"5","author":"LC Rohan","year":"2010","journal-title":"PLoS One"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"F13","DOI":"10.1097\/QAD.0b013e3283522272","article-title":"Unraveling the divergent results of pre-exposure prophylaxis trials for HIV prevention","volume":"26","author":"A van der Straten","year":"2012","journal-title":"AIDS"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1089\/aid.2013.0122","article-title":"Pharmacokinetics and Pharmacodynamics in HIV Prevention; Current Status and Future Directions: A Summary of the DAIDS and BMGF Sponsored Think Tank on Pharmacokinetics (PK)\/Pharmacodynamics (PD) in HIV Prevention","volume":"29","author":"J Romano","year":"2013","journal-title":"AIDS Res Hum Retroviruses"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e55013","DOI":"10.1371\/journal.pone.0055013","article-title":"MTN-001: randomized pharmacokinetic cross-over study comparing tenofovir vaginal gel and oral tablets in vaginal tissue and other compartments","volume":"8","author":"CW Hendrix","year":"2013","journal-title":"PLoS One"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1097\/QAI.0b013e3182717c98","article-title":"Biphasic elimination of tenofovir diphosphate and nonlinear pharmacokinetics of zidovudine triphosphate in a microdosing study","volume":"61","author":"J Chen","year":"2012","journal-title":"J Acquir Immune Defic Syndr"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1093\/jac\/dkq447","article-title":"Pharmacological considerations for tenofovir and emtricitabine to prevent HIV infection","volume":"66","author":"PL Anderson","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1093\/humrep\/deh806","article-title":"Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract","volume":"20","author":"JV Fahey","year":"2005","journal-title":"Hum Reprod"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1086\/340512","article-title":"Effect of menstrual status on anti-bacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture","volume":"185","author":"JV Fahey","year":"2002","journal-title":"J Infect Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.fertnstert.2005.01.104","article-title":"Secretion of monocyte chemotactic protein-1 by human uterine epithelium directs monocyte migration in culture","volume":"84","author":"RA Meter","year":"2005","journal-title":"Fertil Steril"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0002-9440(10)61768-3","article-title":"Fibroblast heterogeneity: existence of functionally distinct Thy 1(+) and Thy 1(-) human female reproductive tract fibroblasts","volume":"159","author":"L Koumas","year":"2001","journal-title":"Am J Pathol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1095\/biolreprod.107.063933","article-title":"Susceptibility of human female primary genital epithelial cells to herpes simplex virus, type-2 and the effect of TLR3 ligand and sex hormones on infection","volume":"77","author":"EM MacDonald","year":"2007","journal-title":"Biol Reprod"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e69854","DOI":"10.1371\/journal.pone.0069854","article-title":"Estradiol regulation of nucleotidases in female reproductive tract epithelial cells and fibroblasts","volume":"8","author":"Z Shen","year":"2013","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e62069","DOI":"10.1371\/journal.pone.0062069","article-title":"Estradiol reduces susceptibility of CD4+ T cells and macrophages to HIV-infection","volume":"8","author":"M Rodriguez-Garcia","year":"2013","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1677\/joe.0.0710077","article-title":"Concentration of oestrogens and androgens in human ovarian venous plasma and follicular fluid throughout the menstrual cycle","volume":"71","author":"KP McNatty","year":"1976","journal-title":"J Endocrinol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"112re114","DOI":"10.1126\/scitranslmed.3003174","article-title":"Penetration of tenofovir and emtricitabine in mucosal tissues: implications for prevention of HIV-1 transmission","volume":"3","author":"KB Patterson","year":"2011","journal-title":"Sci Transl Med"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.1600-0897.1997.tb00311.x","article-title":"Flow cytometric analysis of leukocytes in the human female reproductive tract: Comparison of Fallopian tube, uterus, cervix, and vagina","volume":"38","author":"AL Givan","year":"1997","journal-title":"Am J Reprod Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1016\/S0140-6736(11)61852-7","article-title":"Pre-exposure prophylaxis for HIV prevention: how to predict success","volume":"379","author":"AD Kashuba","year":"2012","journal-title":"Lancet"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/labinvest.2009.14","article-title":"Tenofovir renal toxicity targets mitochondria of renal proximal tubules","volume":"89","author":"JJ Kohler","year":"2009","journal-title":"Lab Invest"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/978-1-61779-163-5_22","article-title":"Subcellular renal proximal tubular mitochondrial toxicity with tenofovir treatment","volume":"755","author":"JJ Kohler","year":"2011","journal-title":"Methods Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1423-0127-20-61","article-title":"Depletion of the cellular antioxidant system contributes to tenofovir disoproxil fumarate - induced mitochondrial damage and increased oxido-nitrosative stress in the kidney","volume":"20","author":"P Abraham","year":"2013","journal-title":"J Biomed Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1600-0897.2010.00842.x","article-title":"Sex hormone regulation of innate immunity in the female reproductive tract: the role of epithelial cells in balancing reproductive potential with protection against sexually transmitted pathogens","volume":"63","author":"CR Wira","year":"2010","journal-title":"Am J Reprod Immunol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.fertnstert.2008.10.047","article-title":"Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female reproductive tract","volume":"92","author":"KD Coleman","year":"2009","journal-title":"Fertil Steril"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/aji.12078","article-title":"Innate immunity in the vagina (part I): estradiol inhibits HBD2 and elafin secretion by human vaginal epithelial cells","volume":"69","author":"MV Patel","year":"2013","journal-title":"Am J Reprod Immunol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e5539","DOI":"10.1371\/journal.pone.0005539","article-title":"Estradiol regulates expression of estrogen receptor ERalpha46 in human macrophages","volume":"4","author":"AJ Murphy","year":"2009","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2005.01.003","article-title":"The 5&apos;-nucleotidases as regulators of nucleotide and drug metabolism","volume":"107","author":"SA Hunsucker","year":"2005","journal-title":"Pharmacol Ther"},{"key":"ref34","unstructured":"Coleman J, Chaturvedula A, Heendrix C, Team M-P (2012) Method of hormonal contraception is associated with lower tenofovir concentration in healthy women (MTN-001): implications for pre-exposure prophylaxis. XIX International AIDS Conference AIDS 2012. Washington, DC."}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0100863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T00:27:03Z","timestamp":1588984023000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0100863"}},"subtitle":[],"editor":[{"given":"Wenzhe","family":"Ho","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,30]]},"references-count":34,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0100863","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,30]]}}}