{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:44:56Z","timestamp":1789436696061,"version":"build-2803163510"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2014,7,9]],"date-time":"2014-07-09T00:00:00Z","timestamp":1404864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0101582","type":"journal-article","created":{"date-parts":[[2014,7,9]],"date-time":"2014-07-09T19:33:19Z","timestamp":1404934399000},"page":"e101582","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Antisense Transcription Regulates the Expression of the Enterohemorrhagic Escherichia coli Virulence Regulatory Gene ler in Response to the Intracellular Iron Concentration"],"prefix":"10.1371","volume":"9","author":[{"given":"Toru","family":"Tobe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilo","family":"Yen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Kagayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naotake","family":"Ogasawara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Oshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,7,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-6445(03)00055-X","article-title":"Bacterial iron homeostasis","volume":"27","author":"SC Andrews","year":"2003","journal-title":"FEMS Microbiol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1126\/science.3287616","article-title":"DNA damage and oxygen radical toxicity","volume":"240","author":"J Imlay","year":"1988","journal-title":"Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1146\/annurev.mi.48.100194.003523","article-title":"Microbial iron transport","volume":"48","author":"ML Guerinot","year":"1994","journal-title":"Annu Rev Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/S1369-5274(00)00184-3","article-title":"Iron and metal regulation in bacteria","volume":"4","author":"K Hantke","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"29478","DOI":"10.1074\/jbc.M303381200","article-title":"Global Iron-dependent Gene Regulation in Escherichia coli","volume":"278","author":"JP McHugh","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1128\/CMR.6.2.137","article-title":"Role of iron in regulation of virulence genes","volume":"6","author":"CM Litwin","year":"1993","journal-title":"Clin Microbiol Rev"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"4759","DOI":"10.1128\/jb.169.10.4759-4764.1987","article-title":"Iron regulation of Shiga-like toxin expression in Escherichia coli is mediated by the fur locus","volume":"169","author":"SB Calderwood","year":"1987","journal-title":"Journal of Bacteriology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.1128\/jb.169.9.4313-4319.1987","article-title":"Nucleotide sequence and promoter mapping of the Escherichia coli Shiga-like toxin operon of bacteriophage H-19B","volume":"169","author":"S De Grandis","year":"1987","journal-title":"Journal of Bacteriology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"JB Kaper","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/IAI.55.1.69-77.1987","article-title":"Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa","volume":"55","author":"S Knutton","year":"1987","journal-title":"Infect Immun"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2958.1998.00783.x","article-title":"The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348\/69","volume":"28","author":"SJ Elliott","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1128\/IAI.66.8.3810-3817.1998","article-title":"Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7","volume":"66","author":"NT Perna","year":"1998","journal-title":"Infect Immun"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4199","DOI":"10.1128\/IAI.01927-06","article-title":"Enteropathogenic and Enterohemorrhagic Escherichia coli Virulence Gene Regulation","volume":"75","author":"JL Mellies","year":"2007","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1099\/mic.0.27100-0","article-title":"Positive effects of multiple pch genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic Escherichia coli O157: H7 to HEp-2 cells","volume":"150","author":"S Iyoda","year":"2004","journal-title":"Microbiology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1128\/JB.187.2.458-472.2005","article-title":"The LEE1 Promoters from both Enteropathogenic and Enterohemorrhagic Escherichia coli Can Be Activated by PerC-Like Proteins from Either Organism","volume":"187","author":"ME Porter","year":"2005","journal-title":"J Bacteriol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1111\/j.1365-2958.2005.04828.x","article-title":"Dual regulatory pathways integrating the RcsC-RcsD-RcsB signalling system control enterohaemorrhagic Escherichia coli pathogenicity","volume":"58","author":"T Tobe","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1365-2958.2006.05217.x","article-title":"ppGpp with DksA controls gene expression in the locus of enterocyte effacement (LEE) pathogenicity island of enterohaemorrhagic Escherichia coli through activation of two virulence regulatory genes","volume":"61","author":"N Nakanishi","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1099\/mic.0.023499-0","article-title":"Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli","volume":"155","author":"N Nakanishi","year":"2009","journal-title":"Microbiology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/dnares\/dsm033","article-title":"Global Regulation by Horizontally Transferred Regulators Establishes the Pathogenicity of Escherichia coli","volume":"15","author":"H Abe","year":"2008","journal-title":"DNA Res"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e20777","DOI":"10.1371\/journal.pone.0020777","article-title":"Regulation of Petrobactin and Bacillibactin Biosynthesis in <italic>Bacillus anthracis<\/italic> under Iron and Oxygen Variation","volume":"6","author":"JY Lee","year":"2011","journal-title":"PLoS ONE"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1007\/BF00269672","article-title":"Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant","volume":"182","author":"K Hantke","year":"1981","journal-title":"Mol Gen Genet"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/prot.22247","article-title":"Ferric uptake regulator protein: Binding free energy calculations and per-residue free energy decomposition","volume":"75","author":"R Ahmad","year":"2009","journal-title":"Proteins: Structure, Function, and Bioinformatics"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1111\/j.1365-2958.2006.05439.x","article-title":"RyhB small RNA modulates the free intracellular iron pool and is essential for normal growth during iron limitation in <italic>Escherichia coli<\/italic>","volume":"62","author":"J-F Jacques","year":"2006","journal-title":"Molecular Microbiology"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"15223","DOI":"10.1073\/pnas.1007805107","article-title":"A small RNA promotes siderophore production through transcriptional and metabolic remodeling","volume":"107","author":"H Salvail","year":"2010","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"13635","DOI":"10.1073\/pnas.93.24.13635","article-title":"Superoxide accelerates DNA damage by elevating free-iron levels","volume":"93","author":"K Keyer","year":"1996","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Gottesman S, Storz G (2010) Bacterial Small RNA Regulators: Versatile Roles and Rapidly Evolving Variations. Cold Spring Harbor Perspectives in Biology 3.","DOI":"10.1101\/cshperspect.a003798"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/nature08756","article-title":"The primary transcriptome of the major human pathogen Helicobacter pylori","volume":"464","author":"CM Sharma","year":"2010","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"20172","DOI":"10.1073\/pnas.1113521108","article-title":"Genome-wide antisense transcription drives mRNA processing in bacteria","volume":"108","author":"I Lasa","year":"2011","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"E709","DOI":"10.1073\/pnas.1101655108","article-title":"Global discovery of small RNAs in Yersinia pseudotuberculosis identifies Yersinia-specific small, noncoding RNAs required for virulence","volume":"108","author":"JT Koo","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"E1277","DOI":"10.1073\/pnas.1201061109","article-title":"The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium","volume":"109","author":"C Kr\u00f6ger","year":"2012","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e1003493","DOI":"10.1371\/journal.pgen.1003493","article-title":"Genome-Wide Identification of Regulatory RNAs in the Human Pathogen <italic>Clostridium difficile<\/italic>","volume":"9","author":"OA Soutourina","year":"2013","journal-title":"PLoS Genet"},{"key":"ref32","volume":"11","unstructured":"Sesto N, Wurtzel O, Archambaud C, Sorek R, Cossart P (2013) The excludon: a new concept in bacterial antisense RNA-mediated gene regulation. Nat Rev Micro\\ <volume>11<\/volume> \\: 75\\-82\\."},{"key":"ref33","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1128\/JB.00926-07","article-title":"Fur Regulates Expression of the Salmonella Pathogenicity Island 1 Type III Secretion System through HilD","volume":"190","author":"JR Ellermeier","year":"2008","journal-title":"J Bacteriol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"e19711","DOI":"10.1371\/journal.pone.0019711","article-title":"Fur Activates the Expression of <italic>Salmonella enterica<\/italic> Pathogenicity Island 1 by Directly Interacting with the <italic>hilD<\/italic> Operator <italic>In Vivo<\/italic> and <italic>In Vitro<\/italic>","volume":"6","author":"L Teixid\u00f3","year":"2011","journal-title":"PLoS ONE"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"KA Datsenko","year":"2000","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2010.06.054","article-title":"Novel aspects of the acid response network of E. coli K-12 are revealed by a study of transcriptional dynamics","volume":"401","author":"NA Burton","year":"2010","journal-title":"J Mol Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3500","DOI":"10.1128\/IAI.70.7.3500-3509.2002","article-title":"Bicarbonate ion stimulates the expression of locus of enterocyte effacement-encoded genes in enterohemorrhagic Escherichia coli O157:H7","volume":"70","author":"H Abe","year":"2002","journal-title":"Infect Immun"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1046\/j.1365-2958.2001.02288.x","article-title":"SoxR-dependent response to oxidative stress and virulence of Erwinia chrysanthemi: the key role of SufC, an orphan ABC ATPase","volume":"39","author":"L Nachin","year":"2001","journal-title":"Mol Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/dnares\/8.1.11","article-title":"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12","volume":"8","author":"T Hayashi","year":"2001","journal-title":"DNA Res"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0101582","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,22]],"date-time":"2020-08-22T03:38:56Z","timestamp":1598067536000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0101582"}},"subtitle":[],"editor":[{"given":"Martin G.","family":"Marinus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,9]]},"references-count":39,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0101582","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,9]]}}}