{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:55:14Z","timestamp":1790434514744,"version":"4.1.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T00:00:00Z","timestamp":1404777600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0101693","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T17:38:57Z","timestamp":1404841137000},"page":"e101693","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Therapeutic Effect of Nanogel-Based Delivery of Soluble FGFR2 with S252W Mutation on Craniosynostosis"],"prefix":"10.1371","volume":"9","author":[{"given":"Masako","family":"Yokota","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiho","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jumpei","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihide","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Akiyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Moriyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,7,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/ng1194-275","article-title":"Jackson-Weiss and Crouzon syndromes are allelic with mutations in fibroblast growth factor receptor 2","volume":"8","author":"EW Jabs","year":"1994","journal-title":"Nat Genet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1038\/ng0896-492","article-title":"Fibroblast growth factor receptor 2 mutations in Beare-Stevenson cutis gyrata syndrome","volume":"13","author":"KA Przylepa","year":"1996","journal-title":"Nat Genet"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/ng0994-98","article-title":"Mutations in the fibroblast growth factor receptor 2 gene cause Crouzon syndrome","volume":"8","author":"W Reardon","year":"1994","journal-title":"Nat Genet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/ng0295-173","article-title":"Identical mutations in the FGFR2 gene cause both Pfeiffer and Crouzon syndrome phenotypes","volume":"9","author":"P Rutland","year":"1995","journal-title":"Nat Genet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/ng0295-165","article-title":"Apert syndrome results from localized mutations of FGFR2 and is allelic with Crouzon syndrome","volume":"9","author":"AO Wilkie","year":"1995","journal-title":"Nat Genet"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"14536","DOI":"10.1073\/pnas.97.26.14536","article-title":"Loss of fibroblast growth factor receptor 2 ligand-binding specificity in Apert syndrome","volume":"97","author":"K Yu","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.bone.2008.02.008","article-title":"The study of abnormal bone development in the Apert syndrome Fgfr2+\/S252W mouse using a 3D hydrogel culture model","volume":"43","author":"F Yang","year":"2008","journal-title":"Bone"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"45926","DOI":"10.1074\/jbc.M404824200","article-title":"A soluble form of fibroblast growth factor receptor 2 (FGFR2) with S252W mutation acts as an efficient inhibitor for the enhanced osteoblastic differentiation caused by FGFR2 activation in Apert syndrome","volume":"279","author":"Y Tanimoto","year":"2004","journal-title":"J Biol Chem"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3267","DOI":"10.1002\/jcp.24021","article-title":"Apert syndrome mutant FGFR2 and its soluble form reciprocally alter osteogenesis of primary calvarial osteoblasts","volume":"227","author":"H Suzuki","year":"2012","journal-title":"J Cell Physiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1002\/dvdy.24099","article-title":"Soluble form of FGFR2 with S252W partially prevents craniosynostosis of the apert mouse model","volume":"243","author":"J Morita","year":"2013","journal-title":"Dev Dyn"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1002\/tcr.201000008","article-title":"Nanogel engineering for new nanobiomaterials: from chaperoning engineering to biomedical applications","volume":"10","author":"Y Sasaki","year":"2010","journal-title":"Chem Rec"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.colsurfb.2011.09.025","article-title":"Dual crosslinked hydrogel nanoparticles by nanogel bottom-up method for sustained-release delivery","volume":"99","author":"A Shimoda","year":"2012","journal-title":"Colloids Surf B Biointerfaces"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1111\/j.1349-7006.2007.00705.x","article-title":"Humoral immune responses in patients vaccinated with 1\u2013146 HER2 protein complexed with cholesteryl pullulan nanogel","volume":"99","author":"S Kageyama","year":"2008","journal-title":"Cancer Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"7397","DOI":"10.1158\/1078-0432.CCR-06-1546","article-title":"HER2-specific T-cell immune responses in patients vaccinated with truncated HER2 protein complexed with nanogels of cholesteryl pullulan","volume":"12","author":"S Kitano","year":"2006","journal-title":"Clin Cancer Res"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.bbrc.2007.12.112","article-title":"Nanogel DDS enables sustained release of IL-12 for tumor immunotherapy","volume":"367","author":"T Shimizu","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5900","DOI":"10.1016\/j.biomaterials.2011.04.058","article-title":"The use of cationic nanogels to deliver proteins to myeloma cells and primary T lymphocytes that poorly express heparan sulfate","volume":"32","author":"K Watanabe","year":"2011","journal-title":"Biomaterials"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/nmat2784","article-title":"Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines","volume":"9","author":"T Nochi","year":"2010","journal-title":"Nat Mater"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"3717","DOI":"10.1182\/blood.V99.10.3717","article-title":"Presentation of a major histocompatibility complex class 1-binding peptide by monocyte-derived dendritic cells incorporating hydrophobized polysaccharide-truncated HER2 protein complex: implications for a polyvalent immuno-cell therapy","volume":"99","author":"Y Ikuta","year":"2002","journal-title":"Blood"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"7613","DOI":"10.1016\/j.biomaterials.2012.06.075","article-title":"Cholesteryl group- and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering","volume":"33","author":"M Fujioka-Kobayashi","year":"2012","journal-title":"Biomaterials"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcp.21760","article-title":"Osteoblastic bone formation is induced by using nanogel-crosslinking hydrogel as novel scaffold for bone growth factor","volume":"220","author":"C Hayashi","year":"2009","journal-title":"J Cell Physiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/0014-4827(54)90169-X","article-title":"A modified technique for organ culture in vitro","volume":"6","author":"OA Trowell","year":"1954","journal-title":"Exp Cell Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1046\/j.0909-8836.1999.eos107406.x","article-title":"Apoptosis in murine calvarial bone and suture development","volume":"107","author":"DP Rice","year":"1999","journal-title":"Eur J Oral Sci"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S8756-3282(03)00222-9","article-title":"A Ser252Trp [corrected] substitution in mouse fibroblast growth factor receptor 2 (Fgfr2) results in craniosynostosis","volume":"33","author":"L Chen","year":"2003","journal-title":"Bone"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ydbio.2009.01.026","article-title":"Early onset of craniosynostosis in an Apert mouse model reveals critical features of this pathology","volume":"328","author":"G Holmes","year":"2009","journal-title":"Dev Biol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1242\/dev.01914","article-title":"Abnormalities in cartilage and bone development in the Apert syndrome FGFR2(+\/S252W) mouse","volume":"132","author":"Y Wang","year":"2005","journal-title":"Development"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"7395","DOI":"10.1074\/jbc.275.10.7395","article-title":"The pyridinyl imidazole inhibitor SB203580 blocks phosphoinositide-dependent protein kinase activity, protein kinase B phosphorylation, and retinoblastoma hyperphosphorylation in interleukin-2-stimulated T cells independently of p38 mitogen-activated protein kinase","volume":"275","author":"FV Lali","year":"2000","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1080\/028443102321096375","article-title":"Correction of a deformed thumb by distraction of the phalanx","volume":"36","author":"K Matsumoto","year":"2002","journal-title":"Scand J Plast Reconstr Surg Hand Surg"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/S0002-9440(10)65401-6","article-title":"Decreased proliferation and altered differentiation in osteoblasts from genetically and clinically distinct craniosynostotic disorders","volume":"154","author":"A Fragale","year":"1999","journal-title":"Am J Pathol"},{"key":"ref29","first-page":"1310","article-title":"Increased calvaria cell differentiation and bone matrix formation induced by fibroblast growth factor receptor 2 mutations in Apert syndrome","volume":"101","author":"A Lomri","year":"1998","journal-title":"J Clin Invest"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"4897","DOI":"10.1074\/jbc.M805432200","article-title":"Fibroblast growth factor receptor 2 promotes osteogenic differentiation in mesenchymal cells via ERK1\/2 and protein kinase C signaling","volume":"284","author":"H Miraoui","year":"2009","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e70240","DOI":"10.1371\/journal.pone.0070240","article-title":"Absence of endochondral ossification and craniosynostosis in posterior frontal cranial sutures of Axin2(\u2212\/\u2212) mice","volume":"8","author":"B Behr","year":"2013","journal-title":"PLoS One"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.molcel.2009.01.025","article-title":"ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing","volume":"33","author":"CC Warzecha","year":"2009","journal-title":"Mol Cell"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"6267","DOI":"10.1073\/pnas.96.11.6267","article-title":"Signaling through fibroblast growth factor receptor 2b plays a key role in the development of the exocrine pancreas","volume":"96","author":"F Miralles","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1086\/302245","article-title":"De novo alu-element insertions in FGFR2 identify a distinct pathological basis for Apert syndrome","volume":"64","author":"M Oldridge","year":"1999","journal-title":"Am J Hum Genet"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.1073\/pnas.071586898","article-title":"A splicing switch and gain-of-function mutation in FgfR2-IIIc hemizygotes causes Apert\/Pfeiffer-syndrome-like phenotypes","volume":"98","author":"MK Hajihosseini","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/S8756-3282(03)00163-7","article-title":"Fgfr mRNA isoforms in craniofacial bone development","volume":"33","author":"DP Rice","year":"2003","journal-title":"Bone"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1242\/dev.127.9.1845","article-title":"Integration of FGF and TWIST in calvarial bone and suture development","volume":"127","author":"DP Rice","year":"2000","journal-title":"Development"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/jez.b.21276","article-title":"Convergent signalling through Fgfr2 regulates divergent craniofacial morphogenesis","volume":"312B","author":"L Veistinen","year":"2009","journal-title":"J Exp Zool B Mol Dev Evol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"ra3","DOI":"10.1126\/scisignal.2002274","article-title":"Wnt\/beta-catenin signaling and AXIN1 regulate apoptosis triggered by inhibition of the mutant kinase BRAFV600E in human melanoma","volume":"5","author":"TL Biechele","year":"2012","journal-title":"Sci Signal"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1097\/01.PRS.0000121182.01199.C1","article-title":"Apoptosis in a rodent model of cranial suture fusion: in situ imaging and gene expression analysis","volume":"113","author":"KD Fong","year":"2004","journal-title":"Plast Reconstr Surg"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.bone.2007.11.019","article-title":"A Pro253Arg mutation in fibroblast growth factor receptor 2 (Fgfr2) causes skeleton malformation mimicking human Apert syndrome by affecting both chondrogenesis and osteogenesis","volume":"42","author":"L Yin","year":"2008","journal-title":"Bone"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1038\/ng2096","article-title":"RNA interference and inhibition of MEK-ERK signaling prevent abnormal skeletal phenotypes in a mouse model of craniosynostosis","volume":"39","author":"V Shukla","year":"2007","journal-title":"Nat Genet"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1111\/j.1601-6343.2009.01460.x","article-title":"Medical treatment of craniosynostosis: recombinant Noggin inhibits coronal suture closure in the rat craniosynostosis model","volume":"12","author":"K Shen","year":"2009","journal-title":"Orthod Craniofac Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1136\/ard.61.8.730","article-title":"Addition of interleukin 1 (IL1) and IL17 soluble receptors to a tumour necrosis factor alpha soluble receptor more effectively reduces the production of IL6 and macrophage inhibitory protein-3alpha and increases that of collagen in an in vitro model of rheumatoid synoviocyte activation","volume":"61","author":"G Chevrel","year":"2002","journal-title":"Ann Rheum Dis"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1158\/1535-7163.MCT-08-0497","article-title":"Therapeutic efficacy of soluble receptor activator of nuclear factor-kappa B-Fc delivered by nonviral gene transfer in a mouse model of osteolytic osteosarcoma","volume":"7","author":"F Lamoureux","year":"2008","journal-title":"Mol Cancer Ther"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/ejhg.2010.235","article-title":"Craniosynostosis","volume":"19","author":"D Johnson","year":"2011","journal-title":"Eur J Hum Genet"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0101693","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T09:37:39Z","timestamp":1716975459000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0101693"}},"subtitle":[],"editor":[{"given":"Pierre J.","family":"Marie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,8]]},"references-count":46,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0101693","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,8]]}}}