{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:34:21Z","timestamp":1790400861443,"version":"4.1.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2014,7,11]],"date-time":"2014-07-11T00:00:00Z","timestamp":1405036800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0102045","type":"journal-article","created":{"date-parts":[[2014,7,11]],"date-time":"2014-07-11T13:22:53Z","timestamp":1405084973000},"page":"e102045","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":106,"title":["Streptococcus pneumoniae in Saliva of Dutch Primary School Children"],"prefix":"10.1371","volume":"9","author":[{"given":"Anne L.","family":"Wyllie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei Ling J. N.","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u00eblle H. B.","family":"Schellens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody","family":"van Engelsdorp Gastelaars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc D.","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arie","family":"van der Ende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debby","family":"Bogaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth A. M.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Trzci\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S1473-3099(04)00938-7","article-title":"Streptococcus pneumoniae colonisation: the key to pneumococcal disease","volume":"4","author":"D Bogaert","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1586\/erv.12.53","article-title":"The fundamental link between pneumococcal carriage and disease","volume":"11","author":"B Simell","year":"2012","journal-title":"Expert Rev Vaccines"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1016\/S0140-6736(10)62225-8","article-title":"Serotype replacement in disease after pneumococcal vaccination","volume":"378","author":"DM Weinberger","year":"2011","journal-title":"Lancet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e1001517","DOI":"10.1371\/journal.pmed.1001517","article-title":"Serotype-specific changes in invasive pneumococcal disease after pneumococcal conjugate vaccine introduction: a pooled analysis of multiple surveillance sites","volume":"10","author":"DR Feikin","year":"2013","journal-title":"PLoS Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.vaccine.2013.08.062","article-title":"Standard method for detecting upper respiratory carriage of Streptococcus pneumoniae: Updated recommendations from the World Health Organization Pneumococcal Carriage Working Group","volume":"32","author":"C Satzke","year":"2013","journal-title":"Vaccine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1097\/00006454-200010000-00019","article-title":"Lack of Utility of Serotyping Multiple Colonies for Detection of Simultaneous Nasopharygeal Carriage of Different Pneumococcal Serotypes","volume":"19","author":"R Huebner","year":"2000","journal-title":"Pediatr Infect Dis J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.vaccine.2013.06.105","article-title":"Design questions for Streptococcus pneumoniae vaccine trials with a colonisation endpoint","volume":"32","author":"K Auranen","year":"2013","journal-title":"Vaccine"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1097\/EDE.0b013e318294be89","article-title":"Competition Between Streptococcus pneumoniae Strains. Implications for Vaccine-Induced Replacement in Colonisation and Disease","volume":"24","author":"J Meht\u00e4l\u00e4","year":"2013","journal-title":"Epidemiology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/aje\/kwp351","article-title":"Between-strain competition in acquisition and clearance of pneumococcal carriage\u2013epidemiologic evidence from a longitudinal study of day-care children","volume":"171","author":"K Auranen","year":"2010","journal-title":"Am J Epidemiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1016\/S0264-410X(00)00046-3","article-title":"Competition among Streptococcus pneumoniae for intranasal colonization in a mouse model","volume":"18","author":"M Lipsitch","year":"2000","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/j.cub.2010.05.051","article-title":"Within-Host Competition Drives Selection for the Capsule Virulence Determinant of Streptococcus pneumoniae","volume":"20","author":"ES Lysenko","year":"2010","journal-title":"Curr Biol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"e1000476","DOI":"10.1371\/journal.ppat.1000476","article-title":"Pneumococcal capsular polysaccharide structure predicts serotype prevalence","volume":"5","author":"DM Weinberger","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref13","first-page":"1","article-title":"Immunosenescence and pneumococcal disease: an imbalance in host\u2013pathogen interactions","volume":"2600","author":"CL Krone","year":"2013","journal-title":"Lancet Respir Med"},{"key":"ref14","unstructured":"Heffron R (1979) Pneumococcus carriers. Pneumonia with special reference to pneumococcus lobar pneumonia. Cambridge, MA: Harvard Univeristy Press. pp. 342\u2013378."},{"key":"ref15","unstructured":"White B (1938) The carrier state. The Biology of Pneumococcus. New York, NY: The Commonwealth Fund. pp. 230\u2013237."},{"key":"ref16","unstructured":"White B (1938) Isolation of pneumococcus. The Biology of Pneumococcus. New York, NY: The Commonwealth Fund. pp. 30\u201364."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.02498-06","article-title":"Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA","volume":"45","author":"MDG Carvalho","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e60520","DOI":"10.1371\/journal.pone.0060520","article-title":"Superiority of trans-oral over trans-nasal sampling in detecting Streptococcus pneumoniae colonization in adults","volume":"8","author":"K Trzci\u0144ski","year":"2013","journal-title":"PLoS One"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1128\/JCM.00157-11","article-title":"Improved Detection of Nasopharyngeal Cocolonization by Multiple Pneumococcal Serotypes by Use of Latex Agglutination or Molecular","volume":"49","author":"P Turner","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1128\/JCM.02243-09","article-title":"Revisiting pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes","volume":"48","author":"MDG Carvalho","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e9282","DOI":"10.1371\/journal.pone.0009282","article-title":"Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease","volume":"5","author":"C Azzari","year":"2010","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e32942","DOI":"10.1371\/journal.pone.0032942","article-title":"Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection","volume":"7","author":"G Biesbroek","year":"2012","journal-title":"PLoS One"},{"key":"ref23","first-page":"124","article-title":"Sequential Multiplex PCR Approach for Determining Capsular Serotypes of Streptococcus pneumoniae Isolates","volume":"44","author":"R Pai","year":"2006"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.7717\/peerj.97","article-title":"Non-pneumococcal mitis-group streptococci confound detection of pneumococcal capsular serotype-specific loci in upper respiratory tract","volume":"1","author":"MDG Carvalho","year":"2013","journal-title":"PeerJ"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1099\/jmm.0.006726-0","article-title":"Multiplex PCR reveals a high rate of nasopharyngeal pneumococcal 7-valent conjugate vaccine serotypes co-colonizing indigenous Warao children in Venezuela","volume":"58","author":"IA Rivera-Olivero","year":"2009","journal-title":"J Med Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"R107","DOI":"10.1186\/gb-2010-11-10-r107","article-title":"Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species","volume":"11","author":"C Donati","year":"2010","journal-title":"Genome Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e2683","DOI":"10.1371\/journal.pone.0002683","article-title":"Evolution of Streptococcus pneumoniae and its close commensal relatives","volume":"3","author":"M Kilian","year":"2008","journal-title":"PLoS One"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1128\/IAI.68.3.1374-1382.2000","article-title":"Genetic Relationships between Clinical Isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: Characterization of Atypical&apos;&apos; Pneumococci and Organisms Allied to S. mitis HarboringS. pneumoniae Virulence Factor-Encoding Gen","volume":"68","author":"AM Whatmore","year":"2000","journal-title":"Infect Immun"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1128\/JCM.01505-12","article-title":"Potential nonpneumococcal confounding of PCR-based determination of serotype in carriage","volume":"50","author":"MDG Carvalho","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"e86136","DOI":"10.1371\/journal.pone.0086136","article-title":"Age-Dependent Prevalence of Nasopharyngeal Carriage of Streptococcus pneumoniae before Conjugate Vaccine Introduction: A Prediction Model Based on a Meta-Analysis","volume":"9","author":"O Le Polain de Waroux","year":"2014","journal-title":"PLoS One"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1007\/BF02183676","article-title":"Untersuchungen \u00fcber das gleichzeitige Vorkommen mehrerer Pneumokokkentypen bei Gesunden und ihre Bedeutung f\u00fcr die Epidemiologie","volume":"114","author":"M Gundel","year":"1933","journal-title":"Zeitschrift f\u00fcr Hyg und Infekt"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1093\/oxfordjournals.aje.a120022","article-title":"An epidemiologic study of pneumonia and its mode of spread","volume":"6","author":"M Rosenau","year":"1926","journal-title":"J Epidemiol"},{"key":"ref33","first-page":"1237","article-title":"Differences in Virulence for Mice among Streptococcus pneumoniae Are Not Attributable to Differences in Pneumolysin Production","volume":"65","author":"KA Benton","year":"1997"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1086\/432513","article-title":"Virulence in mice of pneumococcal clonal types with known invasive disease potential in humans","volume":"192","author":"A Sandgren","year":"2005","journal-title":"J Infect Dis"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1017\/S0022172400040420","article-title":"The significance of pneumococcal types","volume":"64","author":"F Griffith","year":"1927","journal-title":"J Hyg (Lond)"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e89541","DOI":"10.1371\/journal.pone.0089541","article-title":"Genome-Wide Identification of Genes Essential for the Survival of Streptococcus pneumoniae in Human Saliva","volume":"9","author":"LM Verhagen","year":"2014","journal-title":"PLoS One"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"e00092","DOI":"10.1128\/mBio.00092-11","article-title":"Streptococcus pneumoniae is desiccation tolerant and infectious upon rehydration","volume":"2","author":"RL Walsh","year":"2011","journal-title":"MBio"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e00200","DOI":"10.1128\/mBio.00200-12","article-title":"High Levels of Genetic Recombination during Nasopharyngeal Carriage and Biofilm Formation in Streptococcus pneumoniae","volume":"3","author":"LR Marks","year":"2012","journal-title":"MBio"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3201\/eid2002.131220","article-title":"Seven-valent pneumococcal conjugate vaccine and nasopharyngeal microbiota in healthy children","volume":"20","author":"G Biesbroek","year":"2014","journal-title":"Emerg Infect Dis"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/1471-2334-8-173","article-title":"Clustering of serotypes in a longitudinal study of Streptococcus pneumoniae carriage in three day care centres","volume":"8","author":"T Leino","year":"2008","journal-title":"BMC Infect Dis"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1093\/cid\/cit721","article-title":"Seasonal Drivers of Pneumococcal Disease Incidence: Impact of Bacterial Carriage and Viral Activity","volume":"58","author":"DM Weinberger","year":"2014","journal-title":"Clin Infect Dis"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0102045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:46:32Z","timestamp":1588970792000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0102045"}},"subtitle":[],"editor":[{"given":"Herminia","family":"de Lencastre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,11]]},"references-count":41,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0102045","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,11]]}}}