{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T18:15:45Z","timestamp":1772907345276,"version":"3.50.1"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0102702","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T15:45:01Z","timestamp":1406043901000},"page":"e102702","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Evaluation of Cepheid's Xpert MTB\/RIF Test on Pleural Fluid in the Diagnosis of Pleural Tuberculosis in a High Prevalence HIV\/TB Setting"],"prefix":"10.1371","volume":"9","author":[{"given":"John K.","family":"Lusiba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Nakiyingi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J.","family":"Kirenga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Kiragga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Lukande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Nsereko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy","family":"Ssengooba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achilles","family":"Katamba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Worodria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moses L.","family":"Joloba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harriet","family":"Mayanja-Kizza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,7,22]]},"reference":[{"key":"ref1","unstructured":"World Health Organization (2013) Global tuberculosis report 2013. World Health Organization."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1001\/archinte.163.9.1009","article-title":"The growing burden of tuberculosis: global trends and interactions with the HIV epidemic","volume":"163","author":"EL Corbett","year":"2003","journal-title":"Arch Intern Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1378\/chest.100.1.191","article-title":"The impact of human immunodeficiency virus infection on tuberculosis and its control","volume":"100","author":"JM FitzGerald","year":"1991","journal-title":"Chest"},{"key":"ref4","first-page":"316","article-title":"Extrapulmonary tuberculosis","volume":"120","author":"SK Sharma","year":"2004","journal-title":"Indian J Med Res"},{"key":"ref5","first-page":"746","article-title":"Evaluation of suspected tuberculous pleurisy: clinical and diagnostic findings in HIV-1-positive and HIV-negative adults in Uganda","volume":"5","author":"H Luzze","year":"2001","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1378\/chest.06-2063","article-title":"Diagnosis and treatment of tuberculous pleural effusion in 2006","volume":"131","author":"A Gopi","year":"2007","journal-title":"Chest"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1097\/00002030-199301000-00011","article-title":"Pleural effusion, tuberculosis and HIV-1 infection in Kigali, Rwanda","volume":"7","author":"J Batungwanayo","year":"1993","journal-title":"Aids"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s00408-009-9165-3","article-title":"Tuberculous pleural effusion","volume":"187","author":"JM Porcel","year":"2009","journal-title":"Lung"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1183\/09031936.00147507","article-title":"Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?","volume":"31","author":"A Trajman","year":"2008","journal-title":"Eur Respir J"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"85","DOI":"10.5588\/ijtld.12.0236","article-title":"Comparison of polymorphonuclear- and lymphocyte-rich tuberculous pleural effusions","volume":"17","author":"S Bielsa","year":"2013","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1136\/thoraxjnl-2012-202338","article-title":"The yield of different pleural fluid volumes for Mycobacterium tuberculosis culture","volume":"68","author":"F von Groote-Bidlingmaier","year":"2013","journal-title":"Thorax"},{"key":"ref12","first-page":"89","article-title":"Diagnosis of tuberculous pleural effusion","volume":"41","author":"AN Aggarwal","year":"1999","journal-title":"Indian J Chest Dis Allied Sci"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1378\/chest.63.1.88","article-title":"Tuberculous pleurisy","volume":"63","author":"HW Berger","year":"1973","journal-title":"Chest"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1136\/bmj.283.6283.20","article-title":"Diagnosing tuberculous pleural effusion: comparative sensitivity of mycobacterial culture and histopathology","volume":"283","author":"S Kumar","year":"1981","journal-title":"Br Med J (Clin Res Ed)"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1001\/archinte.1970.00310080075009","article-title":"Diagnosis of tuberculous pleurisy by culture of pleural biopsy specimen","volume":"126","author":"H Levine","year":"1970","journal-title":"Arch Intern Med"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1183\/09031936.00067307","article-title":"Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy","volume":"30","author":"M Losi","year":"2007","journal-title":"Eur Respir J"},{"key":"ref17","volume":"30","unstructured":"Pai M, Minion J, Sohn H, Zwerling A, Perkins MD (2009) Novel and improved technologies for tuberculosis diagnosis: progress and challenges. Clin Chest Med <volume>30<\/volume>: : 701\u2013716, viii."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1378\/chest.06-2273","article-title":"Diagnostic value of interferon-gamma in tuberculous pleurisy: a metaanalysis","volume":"131","author":"J Jiang","year":"2007","journal-title":"Chest"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1128\/JCM.01463-09","article-title":"Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology","volume":"48","author":"D Helb","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1128\/JCM.00128-10","article-title":"Evaluation of the analytical performance of the Xpert MTB\/RIF assay","volume":"48","author":"R Blakemore","year":"2010","journal-title":"J Clin Microbiol"},{"key":"ref21","first-page":"CD009593","article-title":"Xpert(R) MTB\/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults","volume":"1","author":"KR Steingart","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"CC Boehme","year":"2010","journal-title":"N Engl J Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1586\/erm.10.67","article-title":"Xpert((R)) MTB\/RIF for point-of-care diagnosis of TB in high-HIV burden, resource-limited countries: hype or hope?","volume":"10","author":"A Van Rie","year":"2010","journal-title":"Expert Rev Mol Diagn"},{"key":"ref24","first-page":"932862","article-title":"Rapid Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in HIV-Infected Patients. Comparison of LED Fluorescent Microscopy and the GeneXpert MTB\/RIF Assay in a District Hospital in India","volume":"2012","author":"G Alvarez-Uria","year":"2012","journal-title":"Tuberc Res Treat"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1128\/JCM.06467-11","article-title":"Effectiveness of an integrated real-time PCR method for detection of the Mycobacterium tuberculosis complex in smear-negative extrapulmonary samples in an area of low tuberculosis prevalence","volume":"50","author":"R Moure","year":"2012","journal-title":"J Clin Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1183\/09031936.00176311","article-title":"Clinical validation of Xpert MTB\/RIF for the diagnosis of extrapulmonary tuberculosis","volume":"40","author":"E Tortoli","year":"2012","journal-title":"Eur Respir J"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1128\/JCM.02268-10","article-title":"Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB\/RIF system","volume":"49","author":"D Hillemann","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1128\/JCM.05454-11","article-title":"Xpert MTB\/RIF assay for diagnosis of pleural tuberculosis","volume":"49","author":"SO Friedrich","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1471-2466-14-58","article-title":"Comparison of same day diagnostic tools including Gene Xpert and unstimulated IFN-gamma for the evaluation of pleural tuberculosis: a prospective cohort study","volume":"14","author":"R Meldau","year":"2014","journal-title":"BMC Pulm Med"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.5588\/ijtld.13.0178","article-title":"Xpert(R) MTB\/RIF in pleural fluid for the diagnosis of tuberculosis","volume":"17","author":"JM Porcel","year":"2013","journal-title":"Int J Tuberc Lung Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"507","DOI":"10.7326\/0003-4819-77-4-507","article-title":"Pleural effusions: the diagnostic separation of transudates and exudates","volume":"77","author":"RW Light","year":"1972","journal-title":"Ann Intern Med"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1136\/thorax.56.11.867","article-title":"Is the pleural fluid transudate or exudate? A revisit of the diagnostic criteria","volume":"56","author":"J Joseph","year":"2001","journal-title":"Thorax"},{"key":"ref33","unstructured":"Strong BE, Kubica GP (1981) Isolation and Identification of Mycobacterium Tuberculosis: A Guide for the Level II Laboratory: Centers for Disease Control."},{"key":"ref34","unstructured":"Siddiqi SH (2006) MGIT Procedure Manual; For BACTEC MGIT 960 TB System, Specially Prepared for FIND MGIT demonstration Project. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.finddiagnostics.org\/export\/sites\/default\/resource-centre\/find_documentation\/pdfs\/mgit_manual_nov_2007.pdf\" xlink:type=\"simple\">http:\/\/www.finddiagnostics.org\/export\/sites\/default\/resource-centre\/find_documentation\/pdfs\/mgit_manual_nov_2007.pdf<\/ext-link>."},{"key":"ref35","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/S1473-3099(06)70602-8","article-title":"Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review","volume":"6","author":"KR Steingart","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"842045","DOI":"10.1155\/2012\/842045","article-title":"A systematic review of the epidemiology, immunopathogenesis, diagnosis, and treatment of pleural TB in HIV-infected patients","volume":"2012","author":"A Aljohaney","year":"2012","journal-title":"Clin Dev Immunol"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0102702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T20:56:19Z","timestamp":1540328179000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0102702"}},"subtitle":[],"editor":[{"given":"Keertan","family":"Dheda","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,22]]},"references-count":36,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0102702","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,22]]}}}