{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T22:32:25Z","timestamp":1788561145633,"version":"build-2803163510"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T00:00:00Z","timestamp":1406505600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0103456","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T18:12:09Z","timestamp":1406571129000},"page":"e103456","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Recombinant Scorpine Produced Using SUMO Fusion Partner in Escherichia coli Has the Activities against Clinically Isolated Bacteria and Inhibits the Plasmodium falciparum Parasitemia In Vitro"],"prefix":"10.1371","volume":"9","author":[{"given":"Chao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlong","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huayun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,7,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0300-9629(95)00058-F","article-title":"Oxygen transport proteins: I. Structure and organization of hemocyanin from scorpion (<italic>Buthus sindicus<\/italic>)","volume":"112","author":"SA Ali","year":"1995","journal-title":"Comp Biochem Physiol"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Ding J, Chua PJ, Bay BH, Gopalakrishnakone P (2014) Scorpion venoms as a potential source of novel cancer therapeutic compounds. Exp Biol Med (Maywood). [Epub ahead of print].","DOI":"10.1177\/1535370213513991"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"29537","DOI":"10.1074\/jbc.271.47.29537","article-title":"Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood","volume":"271","author":"L Ehret-Sabatier","year":"1996","journal-title":"J Biol Chem"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1042\/bj3450653","article-title":"Androctonin, a hydrophilic disulphide-bridged nonhaemolytic anti-microbial peptide: a plausible mode of action","volume":"345","author":"C Hetru","year":"2000","journal-title":"Biochem J"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"5023","DOI":"10.1046\/j.1432-1327.2000.01556.x","article-title":"Hadrurin, a new antimicrobial peptide from the venom of the scorpion <italic>Hadrurus aztecus<\/italic>","volume":"267","author":"A Torres-Larios","year":"2000","journal-title":"Eur J Biochem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"4799","DOI":"10.1046\/j.1432-1033.2002.03177.x","article-title":"Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa","volume":"269","author":"L Moerman","year":"2002","journal-title":"Eur J Biochem"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0014-5793(00)01384-3","article-title":"Scorpine, an anti-malaria and anti-bacterial agent purified from <italic>Scorpion venom<\/italic>","volume":"471","author":"R Conde","year":"2000","journal-title":"FEBS Lett"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.1007\/s00018-008-8250-8","article-title":"Recombinant scorpine: a multifunctional antimicrobial peptide with activity against different pathogens","volume":"65","author":"R Carballar-Lejarazu","year":"2008","journal-title":"Cell Mol Life Sci"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.pep.2008.11.006","article-title":"TrxA mediating fusion expression of antimicrobial peptide CM4 from multiple joined genes in <italic>Escherichia coli<\/italic>","volume":"64","author":"L Zhou","year":"2009","journal-title":"Protein Expression and Purification"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pep.2005.03.016","article-title":"SUMO fusion technology for difficult-to-express proteins","volume":"43","author":"TR Butt","year":"2005","journal-title":"Protein Expr Purif"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00253-007-1330-0","article-title":"Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in <italic>Escherichia coli<\/italic>","volume":"78","author":"Z Sun","year":"2008","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1007\/s00284-011-9891-7","article-title":"Production of Bioactive c-Glutamyl Transpeptidase in <italic>Escherichia coli<\/italic> Using SUMO Fusion Partner and Application of the Recombinant Enzyme to L-Theanine Synthesis","volume":"62(5)","author":"Q Wang","year":"2011","journal-title":"Curr Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1128\/JCM.30.7.1654-1660.1992","article-title":"Detection of <italic>Staphylococcus aureus<\/italic> by polymerase chain reaction amplification of the nuc gene","volume":"30(7)","author":"OG Brakstad","year":"1992","journal-title":"J Clin Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jmii.2010.04.003","article-title":"Polymerase chain reaction assay for the detection of <italic>Acinetobacter baumannii<\/italic> in endotracheal aspirates from patients in the intensive care unit","volume":"44(2)","author":"MC Chiang","year":"2011","journal-title":"J Microbiol Immunol Infect"},{"key":"ref15","unstructured":"CLSI (2009) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M07-A8."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1093\/jac\/37.6.1171","article-title":"Activity of glycylcyclines CL 329998 and CL 331002 against minocycline-resistant and other strains of methicillin resistant <italic>Staphylococcus aureus<\/italic>","volume":"37","author":"JMT Hamilton-Miller","year":"1996","journal-title":"J Antimicrob Chemother"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1126\/science.1199115","article-title":"Development of transgenic fungi that kill human malaria parasites in mosquitoes","volume":"331(6020)","author":"W Fang","year":"2011","journal-title":"Science"},{"key":"ref18","unstructured":"Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. In: A laboratory manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press. 880\u2013898."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"277","author":"UK Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/nprot.2006.4","article-title":"Tricine\u2013SDS-PAGE","volume":"1(1)","author":"H Sch\u00e4gger","year":"2006","journal-title":"Nat Protoc"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5787","DOI":"10.1038\/sj.onc.1209576","article-title":"Human glutathione S-transferase P1-1 interacts with TRAF2 and regulates TRAF2-ASK1 signals","volume":"25","author":"YF Wu","year":"2006","journal-title":"Oncogene"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"6951","DOI":"10.1128\/AEM.70.12.6951-6956.2004","article-title":"Colorimetric method for identifying plant essential oil components that affect biofilm formation and structure","volume":"70","author":"C Niu","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"6167","DOI":"10.4049\/jimmunol.172.10.6167","article-title":"Fusion of Two Malaria Vaccine Candidate Antigens Enhances Product Yield, Immunogenicity, and Antibody-Mediated Inhibition of Parasite Growth In Vitro","volume":"172","author":"W Pan","year":"2004","journal-title":"J Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"W Trager","year":"1976","journal-title":"Science"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2307\/3280287","article-title":"Synchronization of <italic>Plasmodium falciparum<\/italic> erythrocytic stages in culture","volume":"65","author":"C Lambros","year":"1979","journal-title":"J Parasitol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1128\/CMR.15.2.167-193.2002","article-title":"Biofilms: Survival mechanisms of clinically relevant microorganisms","volume":"15","author":"RM Donlan","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/nrmicro2415","article-title":"The biofilm matrix","volume":"8","author":"HC Flemming","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1046\/j.1432-1327.1999.00625.x","article-title":"Scorpion toxins specific for Na<sup>+<\/sup>-channels","volume":"264(2)","author":"LD Possani","year":"1999","journal-title":"Eur J Biochem"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1119(00)00139-6","article-title":"Ubiquitin-like proteins: new wines in new bottles","volume":"248","author":"ET Yeh","year":"2000","journal-title":"Gene"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0962-8924(00)01785-2","article-title":"Ubiquitin and its kin: how close are the family ties?","volume":"10","author":"S Jentsch","year":"2000","journal-title":"Trends Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"6252","DOI":"10.1074\/jbc.275.9.6252","article-title":"Functional heterogeneity of small ubiquitin-related protein modiWers SUMO-1 versus SUMO-2\/3","volume":"275","author":"H Saitoh","year":"2000","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.pep.2005.02.004","article-title":"Expression and puriWcation of SARS coronavirus proteins using SUMO-fusions","volume":"42","author":"Z Xun","year":"2005","journal-title":"Protein Expression and Purication"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/18457","article-title":"A new protease required for cell-cycle progression in yeast","volume":"398","author":"SJ Li","year":"1999","journal-title":"Nature"},{"key":"ref34","first-page":"2367","article-title":"The yeast ULP2 (SMT4) gene encodes a novel protease speciWc for the ubiquitin-like Smt3 protein, Mol Cell Biol","volume":"20","author":"SJ Li","year":"2000"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1541-4337.2003.tb00012.x","article-title":"Biofilm formation and control in food processing facilities","volume":"2","author":"R Chmielewski","year":"2003","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1111\/j.1365-2672.2010.04756.x","article-title":"Biofilm formation and the food industry, a focus on the bacterial outer surface","volume":"109","author":"R Van Houdt","year":"2010","journal-title":"J Appl Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1128\/IAI.64.4.1085-1092.1996","article-title":"Role of the acid tolerance response in virulence of <italic>Salmonella typhimurium<\/italic>","volume":"64","author":"M Riesenberg-Wilmes","year":"1996","journal-title":"Infect Immun"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1046\/j.1365-2672.2003.02140.x","article-title":"Cross-resistance to antibiotics of <italic>Escherichia coli<\/italic> adapted to benzalkonium chloride or exposed to stress-inducers","volume":"96","author":"S Langsrud","year":"2004","journal-title":"J Appl Microbiol"},{"key":"ref39","first-page":"278","article-title":"Characterization of biofilm-forming abilities of antibiotic-resistant <italic>Salmonella typhimurium<\/italic> DT104 on hydrophobic abiotic surfaces","volume":"39","author":"YB Ngwai","year":"2006","journal-title":"J Microbiol Immunol Infect"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4315\/0362-028X-70.1.22","article-title":"Biofilm formation by multidrug-resistant <italic>Salmonella enterica<\/italic> serotype Typhimurium phage type DT104 and other pathogens","volume":"70","author":"SH Kim","year":"2007","journal-title":"J Food Prot"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e1000406","DOI":"10.1371\/journal.pmed.1000406","article-title":"A research agenda to underpin malaria eradication","volume":"8","author":"PL Alonso","year":"2011","journal-title":"PLoS medicine"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0103456","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,22]],"date-time":"2020-08-22T17:25:54Z","timestamp":1598117154000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0103456"}},"subtitle":[],"editor":[{"given":"Mauricio Martins","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,28]]},"references-count":41,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0103456","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,28]]}}}