{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T02:16:42Z","timestamp":1772763402966,"version":"3.50.1"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T00:00:00Z","timestamp":1407715200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0104425","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T18:20:07Z","timestamp":1407781207000},"page":"e104425","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comparative Molecular Dynamics Simulation of Hepatitis C Virus NS3\/4A Protease (Genotypes 1b, 3a and 4a) Predicts Conformational Instability of the Catalytic Triad in Drug Resistant Strains"],"prefix":"10.1371","volume":"9","author":[{"given":"Mitchell","family":"Kramer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Halleran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moazur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mazhar","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Ikram","family":"Anwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salwa","family":"Sabet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Ackad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Yousef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2014,8,11]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/j.1478-3231.2008.01934.x","article-title":"The global burden of hepatitis C","volume":"29","author":"D Levanchy","year":"2009","journal-title":"Liver"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.virusres.2007.10.003","article-title":"A wide range of NS3\/4A protease catalytic efficiencies in HCV- infected individuals","volume":"131","author":"S Franco","year":"2008","journal-title":"Virus Res"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0086413","article-title":"HCV genotypes, characterization of mutations conferring drug resistance to protease inhibitors, and risk factors among blood donors in S\u00e3o Paulo, Brazil","volume":"9","author":"AS Nishiya","year":"2014","journal-title":"PLoS One"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1002\/jmv.23399","article-title":"Distribution of hepatitis C virus genotypes in a diverse US integrated health care population","volume":"84","author":"MM Manos","year":"2012","journal-title":"J Medi Virol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"14757","DOI":"10.1073\/pnas.1008877107","article-title":"Evidence of intense ongoing endemic transmission of hepatitis C virus in Egypt","volume":"107","author":"FD Miller","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"e52467","DOI":"10.1371\/journal.pone.0052467","article-title":"Correlation of viral loads with HCV genotypes: higher levels of virus were revealed among blood donors infected with 6a strains","volume":"7","author":"X Rong","year":"2012","journal-title":"PLoS One"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1016\/j.jhep.2010.11.016","article-title":"Management of hepatitis C virus genotype 4: Recommendations of an international expert panel","volume":"54","author":"MA Khattab","year":"2011","journal-title":"J Hepatol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.cld.2005.05.010","article-title":"Chronic hepatitis C: Genotypes 4 to 9","volume":"9","author":"MH Nguyen","year":"2005","journal-title":"Clin Liver Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1186\/1743-422X-10-351","article-title":"Prevalence of active hepatitis C virus infections among general public of Lahore, Pakistan","volume":"10","author":"MI Anwar","year":"2013","journal-title":"Virol J"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1743-422X-8-55","article-title":"Promiscuous prediction and conservancy analysis of CTL binding epitopes of HCV 3a viral proteasome from Punjab Pakistan: an in silico approach","volume":"8","author":"A Shehzadi","year":"2011","journal-title":"Virol J"},{"key":"ref11","first-page":"1","article-title":"Molecular epidemiology of hepatitis C virus genotypes in Khyber Pakhtoonkhawa of Pakistan","volume":"7","author":"A Ali","year":"2010","journal-title":"Virol J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.3390\/v2081752","article-title":"Hepatitis C virus NS3\/4A protease inhibitors: A light at the end of the tunnel","volume":"2","author":"L Chatel-Chaix","year":"2010","journal-title":"Viruses"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"406","DOI":"10.5009\/gnl.2011.5.4.406","article-title":"The therapeutic approaches for hepatitis C virus: Protease inhibitors and polymerase inhibitors","volume":"4","author":"PY Kwo","year":"2011","journal-title":"Gut Liver"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"5365","DOI":"10.1128\/AAC.01256-12","article-title":"Efficient replication of genotype 3a and 4a hepatitis C virus replicons in human hepatoma cells","volume":"56","author":"M Saeed","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/jmv.2089","article-title":"Inhibition of hepatitis C virus NS3 function by antisense oligodeoxynucleotides and protease inhibitor","volume":"65","author":"T Heintges","year":"2001","journal-title":"J Med Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.3748\/wjg.v8.i6.1088","article-title":"Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease","volume":"8","author":"G Du","year":"2002","journal-title":"World J Gastroenterol"},{"key":"ref17","unstructured":"Lin C (2006) Hepatitis C Viruses: Genomes and Molecular Biology. In: Tan SL, editor. HCV NS3-4A Serine Protease, Norfolk: Horizon Bioscience. pp. 163\u2013206."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1021\/cr000033x","article-title":"Serine protease mechanism and specificity","volume":"102","author":"L Hedstrom","year":"2002","journal-title":"Chem Rev"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0022-2836(73)90103-4","article-title":"The charge relay system in chymotrypsin and chymotrypsinogen","volume":"74","author":"AR Fersht","year":"1973","journal-title":"J Mol Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1021\/cr010184f","article-title":"Viral proteases","volume":"102","author":"L Tong","year":"2002","journal-title":"Chem Rev"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1073\/pnas.71.5.1643","article-title":"Acylation of \u03b1-chymotrypsin by oxygen and sulfur esters of specific substrates: Kinetic evidence for a tetrahedral intermediate","volume":"71","author":"H Hirohara","year":"1974","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"22725","DOI":"10.1074\/jbc.R110.125294","article-title":"Hepatitis C virus non-structural protein 3 (HCV NS3): A multifunctional antiviral target","volume":"285","author":"KD Raney","year":"2010","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1016\/j.bpj.2010.10.037","article-title":"Conformational stability of hepatitis C virus NS3 protease","volume":"99","author":"O Abian","year":"2010","journal-title":"Biophys J"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1006\/prep.1999.1090","article-title":"An uniquely purified HCV NS3 protease and NS4A21\u00b134 peptide form a highly active serine protease complex in peptide hydrolysis","volume":"16","author":"VV Sardana","year":"1999","journal-title":"Protein Expr Purif"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1002\/hep.24172","article-title":"Genotype differences in susceptibility and resistance development of hepatitis C virus to protease inhibitors telaprevir (VX-950) and Danoprevir (ITMN-191)","volume":"53","author":"I Imhof","year":"2011","journal-title":"Hepatology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1080\/07391102.2013.800001","article-title":"A 3D structural model and dynamics of hepatitis C virus NS3\/4A protease (genotype 4a, strain ED43) suggest conformational instability of the catalytic triad: implications in catalysis and drug resistivity","volume":"32","author":"B Rimmert","year":"2014","journal-title":"J Biomol Struct Dyn"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.antiviral.2011.11.007","article-title":"Molecular modeling study on the resistance mechanism of HCV NS3\/4A serine protease mutants R155K, A156V and D168A to TMC435","volume":"93","author":"W Xue","year":"2012","journal-title":"Antiviral Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1016\/j.bbagen.2012.06.001","article-title":"Understanding the drug resistance mechanism of hepatitis C virus NS3\/4A to ITMN-191 due to R155K, A156V, D168A\/E mutations: a computational study","volume":"1820","author":"D Pan","year":"2012","journal-title":"Biochim Biophys Acta"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"5693","DOI":"10.1021\/ct400603p","article-title":"Improving the resistance profile of hepatitis C NS3\/4A inhibitors: Dynamic substrate envelope guided design","volume":"9","author":"A Ozen","year":"2013","journal-title":"J Chem Theory Comput"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1021\/ci400060j","article-title":"Computational study on the drug resistance mechanism against HCV NS3\/4A protease inhibitors vaniprevir and MK-5172 by the combination use of molecular dynamics simulation, residue interaction network, and substrate envelope analysis","volume":"54","author":"W Xue","year":"2014","journal-title":"J Chem Inf Model"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.antiviral.2014.01.010","article-title":"The competitive binding between inhibitors and substrates of HCV NS3\/4A protease: a general mechanism of drug resistance","volume":"103","author":"Y Guan","year":"2014","journal-title":"Antiviral Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1021\/ci400605a","article-title":"Key binding and susceptibility of NS3\/4A serine protease inhibitors against hepatitis C virus","volume":"54","author":"A Meeprasert","year":"2014","journal-title":"J Chem Inf Model"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1053\/j.gastro.2011.06.004","article-title":"Differential efficacy of protease inhibitors against HCV genotypes 2a, 3a, 5a, and 6a NS3\/4A protease recombinant viruses","volume":"141","author":"JM Gottwein","year":"2011","journal-title":"Gastroenterology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1093\/infdis\/jit376","article-title":"Additional glycosylation within a specific hypervariable region of subtype 3a of hepatitis C virus protects against virus neutralization","volume":"208","author":"S Anjum","year":"2013","journal-title":"J Infect Dis"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e39652","DOI":"10.1371\/journal.pone.0039652","article-title":"HCV genotypes are differently prone to the development of resistance to linear and macrocyclic protease inhibitors","volume":"7","author":"V Cento","year":"2012","journal-title":"PLoS One"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1002\/hep.26641","article-title":"Once-daily simeprevir (TMC435) with pegylated interferon and ribavirin in treatment-na\u00efve genotype 1 hepatitis C: The randomized PILLAR study","volume":"58","author":"MW Fried","year":"2013","journal-title":"Hepatology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1056\/NEJMoa1214853","article-title":"Sofosbuvir for previously untreated chronic hepatitis C infection","volume":"368","author":"E Lawitz","year":"2013","journal-title":"N Engl J Med"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1093\/cid\/ciu012","article-title":"Minimum costs for producing hepatitis C direct-acting antivirals for use in large-scale treatment access programs in developing countries","volume":"58","author":"A Hill","year":"2014","journal-title":"Clin Infect Dis"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.tim.2013.09.008","article-title":"Treatment as prevention and cure towards global eradication of hepatitis C virus","volume":"21","author":"LM Hagan","year":"2013","journal-title":"Trends Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1093\/infdis\/jit562","article-title":"In vivo emergence of a novel mutant L159F\/L320F in the NS5B polymerase confers low-level resistance to the HCV polymerase inhibitors Mericitabine and Sofosbuvir","volume":"209","author":"X Tong","year":"2014","journal-title":"J Infect Dis"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1006\/jmbi.1999.2745","article-title":"The solution structure of the N-terminal proteinase domain of the hepatitis C virus (HCV) NS3 protein provides new insights into its activation and catalytic mechanism","volume":"289","author":"G Barbato","year":"1999","journal-title":"J Mol Biol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1006\/jmbi.2000.4365","article-title":"Solution structure and dynamics of the single-chain hepatitis C virus NS3 protease NS4A cofactor complex","volume":"305","author":"MA McCoy","year":"2001","journal-title":"J Mol Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1002\/pro.5560070402","article-title":"Complex of NS3 protease and NS4A peptide of BK strain hepatitis C virus: A 2.2 \u00c5 resolution structure in a hexagonal crystal form","volume":"7","author":"Y Yan","year":"1998","journal-title":"Protein Sci"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"20986","DOI":"10.1073\/pnas.1006370107","article-title":"Drug resistance against HCV NS3\/4A inhibitors is defined by the balance of substrate recognition versus inhibitor binding","volume":"107","author":"KP Romano","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"7152","DOI":"10.1074\/jbc.275.10.7152","article-title":"Inhibition of the hepatitis C virus NS3\/4A protease. The crystal structures of two protease-inhibitor complexes","volume":"275","author":"S Di Marco","year":"2000","journal-title":"J Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/prot.1145","article-title":"Linear programming optimization and a double statistical filter for protein threading protocols","volume":"45","author":"J Meller","year":"2001","journal-title":"Proteins"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL workspace: A web-based environment for protein structure homology modeling","volume":"22","author":"K Arnold","year":"2006","journal-title":"Bioinformatics"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1002\/elps.1150181505","article-title":"SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling","volume":"18","author":"N Guex","year":"1997","journal-title":"Electrophoresis"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"D387","DOI":"10.1093\/nar\/gkn750","article-title":"The SWISS-MODEL repository and associated resources","volume":"37","author":"F Kiefer","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1038\/nbt0795-658","article-title":"Protein modeling by Email","volume":"13","author":"MC Peitsch","year":"1995","journal-title":"Nat Biotechnol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: An automated protein homology-modeling server","volume":"31","author":"T Schwede","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1107\/S0907444903008126","article-title":"A graphical user interface to the CCP4 program suite","volume":"59","author":"E Potterton","year":"2003","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"MD Winn","year":"2011","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref54","unstructured":"Van Gunsteren WF, Billeter SR, Eising AA, Hunenberger PH, Kruger P, <etal>et al<\/etal>. (1996) Biomoleculars simulation: The gromos 96 manual and user guide. Zurich: vdf Hochschulverlag ETH."},{"key":"ref55","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/pro.5560020916","article-title":"Verification of protein structures: Patterns of nonbonded interactions","volume":"2","author":"C Colovos","year":"1993","journal-title":"Protein Sci"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/381272a0","article-title":"Errors in protein structures","volume":"381","author":"RWW Hooft","year":"1996","journal-title":"Nature"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: A program to check stereochemical quality of protein structures","volume":"26","author":"RA Laskowski","year":"1993","journal-title":"J Appl Cryst"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"R Luthy","year":"1992","journal-title":"Nature"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1006\/jmbi.1996.0628","article-title":"Deviations from standard atomic volumes as a quality measure for protein crystal structures","volume":"264","author":"J Pontius","year":"1996","journal-title":"J Mol Biol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0022-2836(63)80023-6","article-title":"Stereochemistry of polypeptide chain configurations","volume":"7","author":"GN Ramachandran","year":"1963","journal-title":"J Mol Biol"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.1021\/jp973084f","article-title":"All atom empirical potential for molecular modeling and dynamics studies of proteins","volume":"102","author":"AD MacKerell","year":"1998","journal-title":"J Phys Chem"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1002\/jcc.20065","article-title":"Extending the treatment of backbone energetics in protein force fields: Limitations of gas-phase quantum mechanics in reproducing protein conformational distributions in molecular dynamics simulations","volume":"25","author":"AD MacKerell","year":"2004","journal-title":"J Comput Chem"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1002\/jcc.20289","article-title":"Scalable molecular dynamics with NAMD","volume":"26","author":"JC Phillips","year":"2005","journal-title":"J Comput Chem"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: Visual molecular dynamics","volume":"14","author":"W Humphrey","year":"1996","journal-title":"J Mol Graph"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of simple potential functions for simulating liquid water","volume":"79","author":"WL Jorgensen","year":"1983","journal-title":"J Phys Chem"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1063\/1.470648","article-title":"Constant pressure molecular dynamics simulation \u2013 the Langevin piston method","volume":"103","author":"SE Feller","year":"1995","journal-title":"J Phy Chem"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0116131","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T00:00:00Z","timestamp":1418947200000}},{"DOI":"10.1371\/journal.pone.0111263","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T00:00:00Z","timestamp":1413331200000}}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0104425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T16:51:42Z","timestamp":1540227102000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0104425"}},"subtitle":[],"editor":[{"given":"Luis","family":"Men\u00e9ndez-Arias","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,8,11]]},"references-count":66,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2014,8,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0104425","relation":{"correction":[{"id-type":"doi","id":"10.1371\/journal.pone.0111263","asserted-by":"object"},{"id-type":"doi","id":"10.1371\/journal.pone.0116131","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,11]]}}}