{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T03:44:21Z","timestamp":1788666261857,"version":"build-2803163510"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T00:00:00Z","timestamp":1408665600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0105374","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T19:24:52Z","timestamp":1408735492000},"page":"e105374","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":41,"title":["Alternative Inactivated Poliovirus Vaccines Adjuvanted with Quillaja brasiliensis or Quil-A Saponins Are Equally Effective in Inducing Specific Immune Responses"],"prefix":"10.1371","volume":"9","author":[{"given":"Fernanda","family":"de Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Carolina A.","family":"Yendo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel P.","family":"Cibulski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane D.","family":"Fleck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo M.","family":"Roehe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando R.","family":"Spilki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Gosmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur G.","family":"Fett-Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,8,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1093\/aje\/kwt203","article-title":"The potential impact of routine immunization with inactivated poliovirus vaccine on wild-type or vaccine-derived poliovirus outbreaks in a posteradication setting","volume":"178","author":"TD Mangal","year":"2013","journal-title":"Am J Epidemiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1098\/rstb.2012.0140","article-title":"The final stages of the global eradication of poliomyelitis","volume":"368","author":"NC Grassly","year":"2013","journal-title":"Philos T R Soc B"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1001\/jama.2013.276583","article-title":"Conflict and polio: Winning the polio wars","volume":"310","author":"ZA Bhutta","year":"2013","journal-title":"JAMA"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1016\/j.vaccine.2010.02.095","article-title":"The global introduction of inactivated polio vaccine can circumvent the oral polio vaccine paradox","volume":"28","author":"E Heinsbroek","year":"2010","journal-title":"Vaccine"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.vaccine.2013.11.101","article-title":"A mucosal adjuvant for the inactivated poliovirus vaccine","volume":"32","author":"BP Steil","year":"2014","journal-title":"Vaccine"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"6971","DOI":"10.1016\/j.vaccine.2012.09.059","article-title":"Adjuvants and inactivated polio vaccine: a systematic review","volume":"30","author":"J Hawken","year":"2012","journal-title":"Vaccine"},{"key":"ref7","unstructured":"Reitz R (1996) Flora Ilustrada Catarinense - Ros\u00e1ceas. Itaja\u00ed: Herb\u00e1rio Barbosa Rodrigues. 135 p."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1080\/14786410310001608055","article-title":"Constituents from leaves of <italic>Quillaja brasiliensis<\/italic>","volume":"18","author":"C Kauffmann","year":"2004","journal-title":"Nat Prod Res"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"7129","DOI":"10.1016\/j.vaccine.2006.06.059","article-title":"Adjuvant activity of <italic>Quillaja brasiliensis<\/italic> saponins on the immune responses to bovine herpesvirus type 1 in mice","volume":"24","author":"JD Fleck","year":"2006","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"9177","DOI":"10.1016\/j.vaccine.2011.09.137","article-title":"<italic>Quillaja brasiliensis<\/italic> saponins are less toxic than Quil A and have similar properties when used as an adjuvant for a viral antigen preparation","volume":"29","author":"F Silveira","year":"2011","journal-title":"Vaccine"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1016\/j.vaccine.2009.01.091","article-title":"Advances in saponin-based adjuvants","volume":"27","author":"H-X Sun","year":"2009","journal-title":"Vaccine"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.biologicals.2010.06.002","article-title":"Mode of action of adjuvants: implications for vaccine safety and design","volume":"38","author":"B Mastelic","year":"2010","journal-title":"Biologicals"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"857","DOI":"10.2174\/138955711796575470","article-title":"Immunoadjuvant and anti-inflammatory plant saponins: characteristics and biotechnological approaches towards sustainable production","volume":"11","author":"F de Costa","year":"2011","journal-title":"Mini Rev Med Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1007\/BF02866642","article-title":"Industrial uses and sustainable supply of <italic>Quillaja saponaria<\/italic> (Rosaceae) saponins","volume":"53","author":"R Mart\u00edn","year":"1999","journal-title":"Econ Bot"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1007\/s11627-009-9222-x","article-title":"Immunoadjuvant saponin production in seedlings and micropropagated plants of <italic>Quillaja brasiliensis<\/italic>","volume":"45","author":"JD Fleck","year":"2009","journal-title":"In Vitro Cell Dev-Pl"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.plaphy.2013.02.003","article-title":"Accumulation of a bioactive triterpene saponin fraction of <italic>Quillaja brasiliensis<\/italic> leaves is associated with abiotic and biotic stresses","volume":"66","author":"F de Costa","year":"2013","journal-title":"Plant Physiol Bioch"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Freshney RI (2010) Subculture and Cell Lines. Culture of Animal Cells: John Wiley &amp; Sons, Inc. pp. 187\u2013206.","DOI":"10.1002\/9780470649367"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1749-6632.1960.tb40933.x","article-title":"A review of theoretical, experimental, and practical considerations in the use of formaldehyde for the inactivation of poliovirus","volume":"83","author":"JE Salk","year":"1960","journal-title":"Ann NY Acad Sci"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.jviromet.2005.06.008","article-title":"A monoclonal antibody-based ELISA allows discrimination between responses induced by bovine herpesvirus subtypes 1 (BoHV-1.1) and 2 (BoHV-1.2)","volume":"129","author":"FR Spilki","year":"2005","journal-title":"J Virol Methods"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/0167-5699(96)80606-2","article-title":"The expanding universe of T-cell subsets: Th1, Th2 and more","volume":"17","author":"TR Mosmann","year":"1996","journal-title":"Immunol Today"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Broere F, Apasov S, Sitkovsky M, Eden W (2011) A2 T cell subsets and T cell-mediated immunity. In: Nijkamp FP, Parnham MJ, editors. Principles of Immunopharmacology: Birkh\u00e4user Basel. pp. 15\u201327.","DOI":"10.1007\/978-3-0346-0136-8_2"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vetimm.2004.10.006","article-title":"Evaluation of bovine cutaneous delayed-type hypersensitivity (DTH) to various test antigens and a mitogen using several adjuvants","volume":"104","author":"A Hern\u00e1ndez","year":"2005","journal-title":"Vet Immunol Immunop"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1086\/315326","article-title":"Mucosal and systemic immunity against poliovirus in mice transgenic for the poliovirus receptor: the poliovirus receptor is necessary for a virus-specific mucosal IgA response","volume":"181","author":"AM Buisman","year":"2000","journal-title":"J Infect Dis"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.4049\/jimmunol.132.6.2736","article-title":"Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin","volume":"132","author":"CO Elson","year":"1984","journal-title":"J Immunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4808","DOI":"10.1016\/j.vaccine.2009.05.081","article-title":"Co-administration of cholera toxin and apple polyphenol extract as a novel and safe mucosal adjuvant strategy","volume":"27","author":"N Yoshino","year":"2009","journal-title":"Vaccine"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"4931","DOI":"10.1128\/JVI.72.6.4931-4939.1998","article-title":"Induction of systemic and mucosal immune responses to human immunodeficiency virus type 1 by a DNA vaccine formulated with QS-21 saponin adjuvant via intramuscular and intranasal routes","volume":"72","author":"S Sasaki","year":"1998","journal-title":"J Virol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.4049\/jimmunol.166.4.2283","article-title":"Oral QS-21 requires early IL-4 help for induction of mucosal and systemic immunity","volume":"166","author":"PN Boyaka","year":"2001","journal-title":"J Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.ijpharm.2012.10.033","article-title":"<italic>Quillaja saponaria<\/italic> extract as mucosal adjuvant with chitosan functionalized gold nanoparticles for mucosal vaccine delivery: stability and immunoefficiency studies","volume":"441","author":"G Barhate","year":"2013","journal-title":"Int J Pharm"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0105374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,16]],"date-time":"2023-07-16T05:49:45Z","timestamp":1689486585000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0105374"}},"subtitle":[],"editor":[{"given":"Mauricio Martins","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,8,22]]},"references-count":29,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2014,8,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0105374","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,22]]}}}