{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:45:54Z","timestamp":1791189954884,"version":"4.1.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2014,8,21]],"date-time":"2014-08-21T00:00:00Z","timestamp":1408579200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0105404","type":"journal-article","created":{"date-parts":[[2014,8,21]],"date-time":"2014-08-21T18:39:12Z","timestamp":1408646352000},"page":"e105404","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Recognition of Bungarus multicinctus Venom by a DNA Aptamer against \u03b2-Bungarotoxin"],"prefix":"10.1371","volume":"9","author":[{"given":"Fengping","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanshui","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,8,21]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"DA Warrell","year":"2010","journal-title":"Lancet"},{"key":"ref2","first-page":"515","article-title":"Snake-bites: appraisal of the global situation","volume":"76","author":"JP Chippaux","year":"1998","journal-title":"Bull World Health Organ"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e218","DOI":"10.1371\/journal.pmed.0050218","article-title":"The Global Burden of Snakebite: A Literature Analysis and Modelling Based on Regional Estimates of Envenoming and Deaths","volume":"5","author":"A Kasturiratne","year":"2008","journal-title":"PLoS Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e2162","DOI":"10.1371\/journal.pntd.0002162","article-title":"The Need for Full Integration of Snakebite Envenoming within a Global Strategy to Combat the Neglected Tropical Diseases: The Way Forward","volume":"7","author":"JM Guti\u00e9rrez","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","first-page":"277","article-title":"Snake Antivenoms: Antivenoms","volume":"41","author":"DG Lalloo","year":"2003","journal-title":"Clin Toxicol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"e150","DOI":"10.1371\/journal.pmed.0030150","article-title":"Confronting the Neglected Problem of Snake Bite Envenoming: The Need for a Global Partnership","volume":"3","author":"JM Guti\u00e9rrez","year":"2006","journal-title":"PLoS Med"},{"key":"ref7","unstructured":"WHO (2010) WHO Guidelines for the production, control and regulation of snake antivenom immunoglobulins 2010 World Health Organization Geneva"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jim.2012.10.016","article-title":"Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody","volume":"387","author":"JF Gao","year":"2013","journal-title":"J Immunol Methods"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.vaccine.2009.10.055","article-title":"Cross-neutralisation of Australian brown snake, taipan and death adder venoms by monovalent antibodies","volume":"28","author":"GK Isbister","year":"2010","journal-title":"Vaccine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0022-1759(03)00277-1","article-title":"Immunogenicity of venoms from four common snakes in the South of Vietnam and development of ELISA kit for venom detection","volume":"282","author":"LV Dong","year":"2003","journal-title":"J Immunol Methods"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S0041-0101(98)00203-7","article-title":"Tests for detection of snake venoms, toxins and venom antibodies: review on recent trends (1987\u20131997)","volume":"37","author":"ZE Selvanayagam","year":"1999","journal-title":"Toxicon"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/nrd3141","article-title":"Aptamers as therapeutics","volume":"9","author":"AD Keefe","year":"2010","journal-title":"Nat Rev Drug Discov"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.3390\/s120201181","article-title":"Applications of aptasensors in clinical diagnostics","volume":"12","author":"P Hong","year":"2012","journal-title":"Sensors"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA that bind specific ligands","volume":"346","author":"AD Ellington","year":"1990","journal-title":"Nature"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"C Tuerk","year":"1990","journal-title":"Science"},{"key":"ref16","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/748913","article-title":"Challenges and opportunities for small molecule aptamer development","volume":"2012","author":"M McKeague","year":"2012","journal-title":"J Nucleic Acids"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/nrmicro1458","article-title":"Aptamers come of age\u2013at last","volume":"4","author":"DH Bunka","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2842","DOI":"10.1021\/cr300468w","article-title":"Aptamers from cell-based selection for bioanalytical applications","volume":"113","author":"W Tan","year":"2013","journal-title":"Chem Rev"},{"key":"ref19","first-page":"518","article-title":"Clinical features of 60 consecutive ICU-treated patients envenomed by Bungarus multicinctus","volume":"40","author":"HT Hung","year":"2009","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"ref20","first-page":"457","article-title":"Evenoming by Bungarus multicinctus (many-banded krait) in Hong Kong","volume":"98","author":"JC Chan","year":"1995","journal-title":"J Trop Med Hyg"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/S0035-9203(97)90524-1","article-title":"Envenoming by Chinese krait (Bungarus multicinctus) and banded krait (B. fasciatus) in Myanmar","volume":"91","author":"T Pe","year":"1997","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref22","unstructured":"Qin GP (1998) China poisonous snake research. Guangxi Science and Technology Press, Nanning. pp. 708\u2013753 [in Chinese]."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1080\/096470490881770","article-title":"Contribution of a snake venom toxin to myasthenia gravis: the discovery of \u03b1-bungarotoxin in Taiwan","volume":"14","author":"NS Chu","year":"2005","journal-title":"J Hist Neurosci"},{"key":"ref24","first-page":"368","article-title":"Looking back on the discovery of \u03b1-bungarotoxin","volume":"6","author":"CC Chang","year":"1999","journal-title":"J Biomed Sci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1093\/oxfordjournals.jbchem.a133844","article-title":"Amino Acid Sequences of Three \u03b2-Bungarotoxins (\u03b23-, \u03b24-, and \u03b25-Bungarotoxins) from Bungarus multicinctus Venom. Amino Acid Substitutions in the A Chains","volume":"91","author":"K Kondo","year":"1982","journal-title":"J Biochem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/neu.480050205","article-title":"Biochemical and physiological properties of a purified snake venom neurotoxin which acts presynaptically","volume":"5","author":"RB Kelly","year":"1974","journal-title":"J Neurobiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0041-0101(89)90134-7","article-title":"Preparation and characterization of monoclonal antibody specific for \u03b1-bungarotoxin and localization of the epitope","volume":"27","author":"LY Chuang","year":"1989","journal-title":"Toxicon"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1073\/pnas.73.1.178","article-title":"beta-Bungarotoxin, a pre-synaptic toxin with enzymatic activity","volume":"73","author":"PN Strong","year":"1976","journal-title":"Proc Natl Acad Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/S0969-2126(01)00246-5","article-title":"Structure of beta2-bungarotoxin: potassium channel binding by Kunitz modules and targeted phospholipase action","volume":"3","author":"PD Kwong","year":"1995","journal-title":"Structure"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/S0041-0101(96)00113-4","article-title":"Mechanism of action of \u03b2-bungarotoxin, a presynaptically acting phospholipase A<sub>2<\/sub> neurotoxin: Its effect on protein phosphorylation in rat brain synaptosomes","volume":"34","author":"E Ueno","year":"1996","journal-title":"Toxicon"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1089\/nat.2012.0377","article-title":"Nucleic acid aptamers against biotoxins: a new paradigm toward the treatment and diagnostic approach","volume":"22","author":"LH Lauridsen","year":"2012","journal-title":"Nucleic Acid Ther"},{"key":"ref32","first-page":"1883","article-title":"Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin","volume":"16","author":"JBH Tok","year":"2008","journal-title":"Chem Commun"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"10456","DOI":"10.1021\/jf801957h","article-title":"Determination of ochratoxin A with a DNA aptamer","volume":"56","author":"JA Cruz-Aguado","year":"2008","journal-title":"J Agric Food Chem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"4937","DOI":"10.1074\/jbc.275.7.4937","article-title":"In vitro selection of RNA molecules that inhibit the activity of ricin A-chain","volume":"275","author":"JR Hesselberth","year":"2000","journal-title":"J Biol Chem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"e41702","DOI":"10.1371\/journal.pone.0041702","article-title":"Rapid One-Step Selection Method for Generating Nucleic Acid Aptamers: Development of a DNA Aptamer against \u03b1-Bungarotoxin","volume":"7","author":"LH Lauridsen","year":"2012","journal-title":"PloS one"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"48644","DOI":"10.1074\/jbc.M104651200","article-title":"Tenascin-C aptamers are generated using tumor cells and purified protein","volume":"276","author":"BJ Hicke","year":"2001","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1073\/pnas.0813135106","article-title":"Micromagnetic selection of aptamers in microfluidic channels","volume":"106","author":"X Lou","year":"2009","journal-title":"Proc Natl Acad Sci"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s00216-012-6183-4","article-title":"Comparison of different methods for generation of single-stranded DNA for SELEX processes","volume":"404","author":"M Svobodov\u00e1","year":"2012","journal-title":"Anal Bioanal Chem"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1073\/pnas.77.2.857","article-title":"recA protein-catalyzed strand assimilation: stimulation by Escherichia coli single-stranded DNA-binding protein","volume":"77","author":"K McEntee","year":"1980","journal-title":"Proc Natl Acad Sci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1073\/pnas.95.6.2902","article-title":"High affinity ligands from in vitro selection: complex targets","volume":"95","author":"KN Morris","year":"1998","journal-title":"Proc Natl Acad Sci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1042\/BJ20061216","article-title":"In vitro selection and characterization of RNA aptamers binding thyroxine hormone","volume":"403","author":"D Levesque","year":"2007","journal-title":"Biochem J"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"5380","DOI":"10.1016\/j.bmc.2009.06.044","article-title":"ssDNA aptamers that recognize diclofenac and 2-anilinophenylacetic acid","volume":"17","author":"CB Joeng","year":"2009","journal-title":"Bioorg Med Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1021\/ja037832s","article-title":"In vitro evolution of functional DNA using capillary electrophoresis","volume":"126","author":"SD Mendonsa","year":"2004","journal-title":"J Am Chem Soc"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s11010-007-9555-x","article-title":"Selection of DNA aptamers against DC-SIGN protein","volume":"306","author":"Y Hui","year":"2007","journal-title":"Mol Cell Biochem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1021\/jf400880r","article-title":"Selection and identification of a DNA aptamer that mimics saxitoxin in antibody binding","volume":"61","author":"P Hu","year":"2013","journal-title":"J Agric Food Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/0022-2836(91)90932-V","article-title":"Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton","volume":"221","author":"PC Ryan","year":"1991","journal-title":"J Mol Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1039\/B614138B","article-title":"Aptamer-based analysis of angiogenin by fluorescence anisotropy","volume":"132","author":"W Li","year":"2007","journal-title":"Analyst"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1016\/0041-0101(91)90194-V","article-title":"DNase activity in Costa Rican crotaline snake venoms: quantification of activity and identification of electrophoretic variants","volume":"29","author":"A Sittenfeld","year":"1991","journal-title":"Toxicon"},{"key":"ref49","first-page":"469","article-title":"Hydrolysis of DNA by 17 snake venoms","volume":"135","author":"AR de Roodt","year":"2003","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0105404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T05:18:37Z","timestamp":1565759917000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0105404"}},"subtitle":[],"editor":[{"given":"Mark","family":"Isalan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,8,21]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2014,8,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0105404","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,21]]}}}