{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:03:26Z","timestamp":1792098206372,"version":"4.4.0"},"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2014,8,26]],"date-time":"2014-08-26T00:00:00Z","timestamp":1409011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0105929","type":"journal-article","created":{"date-parts":[[2014,8,26]],"date-time":"2014-08-26T18:20:26Z","timestamp":1409077226000},"page":"e105929","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Tumour Microenvironments Induce Expression of Urokinase Plasminogen Activator Receptor (uPAR) and Concomitant Activation of Gelatinolytic Enzymes"],"prefix":"10.1371","volume":"9","author":[{"given":"Synn\u00f8ve","family":"Magnussen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elin","family":"Hadler-Olsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Latysheva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Pirila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja E.","family":"Steigen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Hanes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Olof","family":"Winberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Uhlin-Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunbj\u00f8rg","family":"Svineng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,8,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/hed.10004","article-title":"Presentation, treatment, and outcome of oral cavity cancer: a National Cancer Data Base report","volume":"24","author":"GF Funk","year":"2002","journal-title":"Head Neck"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1172\/JCI59889","article-title":"The molecular pathogenesis of head and neck squamous cell carcinoma","volume":"122","author":"SM Rothenberg","year":"2012","journal-title":"J Clin Invest"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3322\/caac.20107","article-title":"Global cancer statistics","volume":"61","author":"A Jemal","year":"2011","journal-title":"CA Cancer J Clin"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3322\/caac.20138","article-title":"Cancer statistics, 2012","volume":"62","author":"R Siegel","year":"2012","journal-title":"CA Cancer J Clin"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s000180050497","article-title":"The plasminogen activation system in tumor growth, invasion, and metastasis","volume":"57","author":"PA Andreasen","year":"2000","journal-title":"Cell Mol Life Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2174\/1381612043453559","article-title":"The urokinase plasminogen activator system: role in malignancy","volume":"10","author":"MJ Duffy","year":"2004","journal-title":"Curr Pharm Des"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1016\/j.febslet.2009.12.039","article-title":"The urokinase receptor: focused cell surface proteolysis, cell adhesion and signaling","volume":"584","author":"F Blasi","year":"2010","journal-title":"FEBS Lett"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1600-0714.2010.00953.x","article-title":"Survey of risk factors contributed to lymphatic metastasis in patients with oral tongue cancer by immunohistochemistry","volume":"40","author":"Z Zhang","year":"2011","journal-title":"J Oral Pathol Med"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1080\/00016489950180342","article-title":"Increased levels of urokinase receptor in plasma of head and neck squamous cell carcinoma patients","volume":"119","author":"M Schmidt","year":"1999","journal-title":"Acta Otolaryngol"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Magnussen S, Rikardsen O, Hadler-Olsen E, Uhlin-Hansen L, Steigen SE, et al. (2014) Urokinase plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1) are potential predictive biomarkers in early stage oral squamous cell carcinomas (OSCC) PLoS One In Press.","DOI":"10.1371\/journal.pone.0101895"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/nrm2821","article-title":"Regulation of cell signalling by uPAR","volume":"11","author":"HW Smith","year":"2010","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Svineng G, Magnussen S, Hadler-Olsen E (2012) Plasmin and the plasminogen activator system in health and disease. In: Karamanos NK, editor. Extracellular Matrix: Pathobiology and Signaling. Germany: Walter de Gruyter GmbH &amp; Co. 261\u2013290.","DOI":"10.1515\/9783110258776.261"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/nrm977","article-title":"uPAR: a versatile signalling orchestrator","volume":"3","author":"F Blasi","year":"2002","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"18224","DOI":"10.1016\/S0021-9258(19)37176-5","article-title":"Urokinase plasminogen activator cleaves its cell surface receptor releasing the ligand-binding domain","volume":"267","author":"G Hoyer-Hansen","year":"1992","journal-title":"J Biol Chem"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1055\/s-0037-1613202","article-title":"Metalloproteases cleave the urokinase-type plasminogen activator receptor in the D1\u2013D2 linker region and expose epitopes not present in the intact soluble receptor","volume":"88","author":"A Andolfo","year":"2002","journal-title":"Thromb Haemost"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1042\/bj3580673","article-title":"Urokinase-catalysed cleavage of the urokinase receptor requires an intact glycolipid anchor","volume":"358","author":"G Hoyer-Hansen","year":"2001","journal-title":"Biochem J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1160\/TH04-09-0580","article-title":"Soluble and cleaved forms of the urokinase-receptor: degradation products or active molecules?","volume":"93","author":"N Montuori","year":"2005","journal-title":"Thromb Haemost"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"22885","DOI":"10.1074\/jbc.271.37.22885","article-title":"Domain interplay in the urokinase receptor. Requirement for the third domain in high affinity ligand binding and demonstration of ligand contact sites in distinct receptor domains","volume":"271","author":"N Behrendt","year":"1996","journal-title":"J Biol Chem"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1432-1033.1997.0021a.x","article-title":"Cell-surface acceleration of urokinase-catalyzed receptor cleavage","volume":"243","author":"G Hoyer-Hansen","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"7279","DOI":"10.1093\/emboj\/16.24.7279","article-title":"A urokinase-sensitive region of the human urokinase receptor is responsible for its chemotactic activity","volume":"16","author":"F Fazioli","year":"1997","journal-title":"EMBO J"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1073\/pnas.022652999","article-title":"The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1\/LXA4R","volume":"99","author":"M Resnati","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"46932","DOI":"10.1074\/jbc.M207494200","article-title":"The cleavage of the urokinase receptor regulates its multiple functions","volume":"277","author":"N Montuori","year":"2002","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"5739","DOI":"10.4049\/jimmunol.173.9.5739","article-title":"Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2","volume":"173","author":"A de Paulis","year":"2004","journal-title":"J Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/(SICI)1097-4652(199908)180:2<225::AID-JCP10>3.0.CO;2-2","article-title":"Cellular glycosylphosphatidylinositol-specific phospholipase D regulates urokinase receptor shedding and cell surface expression","volume":"180","author":"OG Wilhelm","year":"1999","journal-title":"J Cell Physiol"},{"key":"ref25","first-page":"540","article-title":"Proteolytic regulation of the urokinase receptor\/CD87 on monocytic cells by neutrophil elastase and cathepsin G. J Immunol","volume":"172","author":"N Beaufort","year":"2004"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/S0021-9258(18)52382-6","article-title":"Cellular receptor for urokinase plasminogen activator. Carboxyl-terminal processing and membrane anchoring by glycosyl-phosphatidylinositol","volume":"266","author":"M Ploug","year":"1991","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1002\/ijc.2910580622","article-title":"A cleaved form of the receptor for urokinase-type plasminogen activator in invasive transplanted human and murine tumors","volume":"58","author":"H Solberg","year":"1994","journal-title":"Int J Cancer"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/S0014-5793(00)01624-0","article-title":"Shedding and cleavage of the urokinase receptor (uPAR): identification and characterisation of uPAR fragments in vitro and in vivo","volume":"475","author":"N Sidenius","year":"2000","journal-title":"FEBS Lett"},{"key":"ref29","first-page":"3294","article-title":"Soluble receptor for urokinase plasminogen activator in both full-length and a cleaved form is present in high concentration in cystic fluid from ovarian cancer","volume":"58","author":"K Wahlberg","year":"1998","journal-title":"Cancer Res"},{"key":"ref30","first-page":"1132","article-title":"Prognostic significance of soluble urokinase plasminogen activator receptor in serum and cytosol of tumor tissue from patients with primary breast cancer","volume":"8","author":"R Riisbro","year":"2002","journal-title":"Clin Cancer Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1373\/clinchem.2005.064253","article-title":"Enhanced discrimination of benign from malignant prostatic disease by selective measurements of cleaved forms of urokinase receptor in serum","volume":"52","author":"T Piironen","year":"2006","journal-title":"Clin Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.lungcan.2011.05.008","article-title":"Urokinase receptor forms in serum from non-small cell lung cancer patients: relation to prognosis","volume":"74","author":"CE Almasi","year":"2011","journal-title":"Lung Cancer"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1002\/pros.21306","article-title":"Prognostic and predictive value of intact and cleaved forms of the urokinase plasminogen activator receptor in metastatic prostate cancer","volume":"71","author":"CE Almasi","year":"2011","journal-title":"Prostate"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1600-0463.2012.02955.x","article-title":"The liberated domain I of urokinase plasminogen activator receptor\u2013a new tumour marker in small cell lung cancer","volume":"121","author":"CE Almasi","year":"2013","journal-title":"APMIS"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1373\/clinchem.2010.144410","article-title":"A new assay for measurement of the liberated domain I of the urokinase receptor in plasma improves the prediction of survival in colorectal cancer","volume":"56","author":"T Thurison","year":"2010","journal-title":"Clin Chem"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1093\/jnci\/91.10.869","article-title":"Plasma urokinase receptor levels in patients with colorectal cancer: relationship to prognosis","volume":"91","author":"RW Stephens","year":"1999","journal-title":"J Natl Cancer Inst"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1093\/clinchem\/43.10.1868","article-title":"ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients","volume":"43","author":"RW Stephens","year":"1997","journal-title":"Clin Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/sj.bjc.6605520","article-title":"Co-detection of members of the urokinase plasminogen activator system in tumour tissue and serum correlates with a poor prognosis for soft-tissue sarcoma patients","volume":"102","author":"H Taubert","year":"2010","journal-title":"Br J Cancer"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.2353\/ajpath.2009.081110","article-title":"A novel organotypic model mimics the tumor microenvironment","volume":"175","author":"S Nurmenniemi","year":"2009","journal-title":"Am J Pathol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2948","DOI":"10.1002\/ijc.21568","article-title":"Expression of plasminogen activator inhibitor-1, urokinase receptor and laminin gamma-2 chain is an early coordinated event in incipient oral squamous cell carcinoma","volume":"118","author":"P Lindberg","year":"2006","journal-title":"Int J Cancer"},{"key":"ref41","first-page":"1444","article-title":"Beta-fibroblast growth factor expression in human and murine squamous cell carcinomas and its relationship to regional endothelial cell proliferation","volume":"53","author":"S Schultz-Hector","year":"1993","journal-title":"Cancer Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.yexcr.2012.12.010","article-title":"The hypoxic tumor microenvironment regulates invasion of aggressive oral carcinoma cells","volume":"319","author":"S Teppo","year":"2013","journal-title":"Exp Cell Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1042\/bj3100345","article-title":"Transcriptional and post-transcriptional regulation of the receptor for urokinase-type plasminogen activator by cytokines and tumour promoters in the human lung carcinoma cell line A549","volume":"1)","author":"LR Lund","year":"1995","journal-title":"Biochem J 310 (Pt"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.4161\/cc.5.16.2994","article-title":"Urokinase-receptor (u-PAR): an essential player in multiple games of cancer: a review on its role in tumor progression, invasion, metastasis, proliferation\/dormancy, clinical outcome and minimal residual disease","volume":"5","author":"S Laufs","year":"2006","journal-title":"Cell Cycle"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/BF01488206","article-title":"Modulation of the receptor for urokinase-type plasminogen activator in macrophage-like U937 cells by inflammatory mediators","volume":"20","author":"E Yoshida","year":"1996","journal-title":"Inflammation"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0014-5793(92)80050-Q","article-title":"Okadaic acid strongly increases gene transcription, mRNA and protein level for the urokinase receptor in human A549 cells","volume":"298","author":"LR Lund","year":"1992","journal-title":"FEBS Lett"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.oraloncology.2012.12.014","article-title":"Molecular communication between tumor-associated fibroblasts and head and neck squamous cell carcinoma","volume":"49","author":"G Leef","year":"2013","journal-title":"Oral Oncol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/joim.12018","article-title":"Anti-angiogenesis and metastasis: a tumour and stromal cell alliance","volume":"273","author":"L Moserle","year":"2013","journal-title":"J Intern Med"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s00109-013-1021-5","article-title":"The metastasis-promoting roles of tumor-associated immune cells","volume":"91","author":"HA Smith","year":"2013","journal-title":"J Mol Med (Berl)"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1159\/000055531","article-title":"Transforming growth factor beta induces urokinase receptor expression in cultured retinal pigment epithelial cells","volume":"31","author":"V Siren","year":"1999","journal-title":"Ophthalmic Res"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ygyno.2005.09.038","article-title":"EGF-stimulated migration in ovarian cancer cells is associated with decreased internalization, increased surface expression, and increased shedding of the urokinase plasminogen activator receptor","volume":"101","author":"E Henic","year":"2006","journal-title":"Gynecol Oncol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.2174\/138161211796718224","article-title":"Regulation of the urokinase receptor (uPAR) by LDL receptor-related protein-1 (LRP1)","volume":"17","author":"SL Gonias","year":"2011","journal-title":"Curr Pharm Des"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.2741\/3365","article-title":"The collagen receptor uPARAP\/Endo180","volume":"14","author":"LH Engelholm","year":"2009","journal-title":"Front Biosci"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"10982","DOI":"10.1074\/jbc.M109.093492","article-title":"Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy","volume":"285","author":"L Lin","year":"2010","journal-title":"J Biol Chem"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"13933","DOI":"10.1074\/jbc.273.22.13933","article-title":"Glycosylation profile of a recombinant urokinase-type plasminogen activator receptor expressed in Chinese hamster ovary cells","volume":"273","author":"M Ploug","year":"1998","journal-title":"J Biol Chem"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"11152","DOI":"10.1016\/S0021-9258(18)82104-4","article-title":"N-linked glycosylation of the ligand-binding domain of the human urokinase receptor contributes to the affinity for its ligand","volume":"268","author":"LB Moller","year":"1993","journal-title":"J Biol Chem"},{"key":"ref57","first-page":"1315","article-title":"Differential expression of a truncated form of the urokinase-type plasminogen-activator receptor in normal and tumor thyroid cells","volume":"58","author":"P Ragno","year":"1998","journal-title":"Cancer Res"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"6453","DOI":"10.1016\/S0021-9258(19)39348-2","article-title":"The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants","volume":"265","author":"N Behrendt","year":"1990","journal-title":"J Biol Chem"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/S0014-5793(99)01314-9","article-title":"Cleavage of urokinase receptor regulates its interaction with integrins in thyroid cells","volume":"460","author":"N Montuori","year":"1999","journal-title":"FEBS Lett"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"12595","DOI":"10.1074\/jbc.M109.008581","article-title":"A cleavage-resistant urokinase plasminogen activator receptor exhibits dysregulated cell-surface clearance","volume":"285","author":"EC Nieves","year":"2010","journal-title":"J Biol Chem"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nrd1751","article-title":"Glycans in cancer and inflammation\u2013potential for therapeutics and diagnostics","volume":"4","author":"DH Dube","year":"2005","journal-title":"Nat Rev Drug Discov"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"46692","DOI":"10.1074\/jbc.M308808200","article-title":"Soluble urokinase-type plasminogen activator receptor inhibits cancer cell growth and invasion by direct urokinase-independent effects on cell signaling","volume":"278","author":"M Jo","year":"2003","journal-title":"J Biol Chem"},{"key":"ref63","first-page":"185","article-title":"suPAR, a soluble form of urokinase plasminogen activator receptor, inhibits human prostate cancer cell growth and invasion","volume":"32","author":"M Piccolella","year":"2008","journal-title":"Int J Oncol"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1038\/sj.bjc.6605642","article-title":"Guidelines for the welfare and use of animals in cancer research","volume":"102","author":"P Workman","year":"2010","journal-title":"Br J Cancer"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.joca.2012.02.010","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research","volume":"20","author":"C Kilkenny","year":"2012","journal-title":"Osteoarthritis Cartilage"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"e70925","DOI":"10.1371\/journal.pone.0070925","article-title":"Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro","volume":"8","author":"CC Bitu","year":"2013","journal-title":"PLoS One"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1034\/j.1601-0825.2003.00968.x","article-title":"Clinical and pathological features of the murine AT-84 orthotopic model of oral cancer","volume":"9","author":"E Lou","year":"2003","journal-title":"Oral Dis"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1247\/csf.13.281","article-title":"Novel purification of vitronectin from human plasma by heparin affinity chromatography","volume":"13","author":"T Yatohgo","year":"1988","journal-title":"Cell Struct Funct"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1369\/jhc.2009.954354","article-title":"Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity","volume":"58","author":"E Hadler-Olsen","year":"2010","journal-title":"J Histochem Cytochem"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels","volume":"68","author":"A Shevchenko","year":"1996","journal-title":"Anal Chem"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0105929","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T16:15:00Z","timestamp":1650039300000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0105929"}},"subtitle":[],"editor":[{"given":"Nikos K.","family":"Karamanos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,8,26]]},"references-count":71,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2014,8,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0105929","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,26]]}}}