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Infect Immun","volume":"14","author":"MW Eklund","year":"1976"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1099\/00221287-1-1-91","article-title":"The toxins of <italic>Clostridium oedematiens<\/italic> (<italic>Cl. novyi<\/italic>)","volume":"1","author":"CL Oakley","year":"1947","journal-title":"J Gen Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.anaerobe.2004.04.006","article-title":"Cloning and molecular characterization of the beta toxin (phospholipase C) gene of <italic>Clostridium haemolyticum<\/italic>","volume":"10","author":"PJ Hauer","year":"2004","journal-title":"Anaerobe"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1099\/0022-1317-51-11-971","article-title":"Lethal outbreak of infection with <italic>Clostridium novyi<\/italic> type A and other spore-forming organisms in Scottish injecting drug users","volume":"51","author":"CC McGuigan","year":"2002","journal-title":"J Med Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1177\/104063879700900319","article-title":"Apparent <italic>Clostridium haemolyticum\/Clostridium novyi<\/italic> infection and exotoxemia in two horses","volume":"9","author":"JL Oaks","year":"1997","journal-title":"J Vet Diagn Invest"},{"key":"ref14","first-page":"365","article-title":"Outbreak of <italic>Clostridium novyi<\/italic> infection in swine and its rapid diagnosis","volume":"40","author":"H Itoh","year":"1987","journal-title":"Nippon Juishikai Zasshi"},{"key":"ref15","first-page":"293","article-title":"Bacillary hemoglobinuria in a free-ranging elk calf","volume":"30","author":"LC Bender","year":"1999","journal-title":"J Zoo Wildl Med"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1111\/j.1751-0813.2005.tb15635.x","article-title":"Acute post-parturient haemoglobinuria in dairy cows and phosphorus status","volume":"83","author":"CR Stockdale","year":"2005","journal-title":"Aust Vet J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1292\/jvms.10-0231","article-title":"Bacillary hemoglobinuria in Japanese black cattle in Hiroshima, Japan: a case study","volume":"73","author":"Y Shinozuka","year":"2011","journal-title":"J Vet Med Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1038\/nbt1256","article-title":"The genome and transcriptomes of the anti-tumor agent <italic>Clostridium novyi-NT<\/italic>","volume":"24","author":"C Bettegowda","year":"2006","journal-title":"Nat Biotechnol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"17472","DOI":"10.1073\/pnas.0505503102","article-title":"The genome sequence of <italic>Clostridium botulinum<\/italic> type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny","volume":"102","author":"Y Sakaguchi","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref20","first-page":"3282","article-title":"Molecular analysis of an extrachromosomal element containing the C2 toxin gene discovered in <italic>Clostridium botulinum<\/italic> type C. 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Anza","year":"2014","journal-title":"Anaerobe"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"A Bankevich","year":"2012","journal-title":"J Comput Biol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2870","DOI":"10.1093\/bioinformatics\/bts563","article-title":"COPE: an accurate k-mer-based pair-end reads connection tool to facilitate genome assembly","volume":"28","author":"B Liu","year":"2012","journal-title":"Bioinformatics"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"R12","DOI":"10.1186\/gb-2004-5-2-r12","article-title":"Versatile and open software for comparing large genomes","volume":"5","author":"S Kurtz","year":"2004","journal-title":"Genome Biol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1128\/AAC.12.3.423","article-title":"Elimination of 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